Status
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Gene information
General information
TC007561 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3010468..3016805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007561
    iBeetle screen iB_01219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007561 RA
      MGGRSKKMD LVRTNSICP PPETVLLES VKKNDTESM 
      KTVIKANPS ILQHLYPYT SQTILSLAC SEPGVAPDT 
      VNQLIELGA DIEGTPQWK PLHLSADNP NILILEVVI 
      KHLKPGQIN EKWNGNTAL HTLIKGEKI KKDEENFCR 
      HVELLLQSG IDVNQGDGK NLTAIFWAA KYGYKKIVK 
      VILEESVLH VDLDTCSLR DKTARDLIN EKGLYEGPL 
      PERILYQVP KDKIFGLIK EGKEDEFIS YFETLSLNN 
      PSDFVNADD GASTLLQYS CERGLAKVV EYLLDKGAN 
      VNLVTKNQR KPIDIVADI GYFEIFELL FKCPDLELS 
      IDTLCNLLK HSNSPKFGK INHEKCCKL LLDKLGNRR 
      PPIDINEVD HLNNIPLHY SLRYCDTTT TQKLLQLGA 
      SLAYKNEFG SMPIQDIKP EVLENHLDN CVTFDLKNA 
      EKRDFEVVF DYQTLLPPR KRKRFKYEE VDPEFLATN 
      NVKPETEVI AYMSKAPEF RALLKHPLI VSFLFMKWH 
      QIRLLFYTN LVFYICFVL SLVVYIFTH YANFDRTQS 
      DYCLIFGKF SWFTLNLTF WVLVLREVF QVAVAPRKY 
      FCNFENLVE IILIVVTGM ILYIDSPTS HTRRQLASV 
      AILLAAFEL VLMVGQHPK LSTNVVMLK TVSVNFFKL 
      LLWYSLLII AFALSFYIL FAKTEMAQS VNGTETGDE 
      DDVFKGPGK SLFKTIVML TGEFDAGSI NFHTYPVTS 
      KIIFSLFVF MITIILLNL LNGLAVSDT QTIKNDAEL 
      VGHISRAQH IYYVESMLL GNILPTSFI QAVQRLFCC 
      CPCDSDTTY TFFKPLSRK VCLFTQNCQ LTVLPNEYG 
      KISCELNSS AKKRPDPLV TCTRNCSEA YLDKNTIRR 
      IADIVKARR EREEYPTVT NLKQLYGEI VSIKAKLDQ 
      ILGSLSTNQ NAL
    CDS sequence
    • >TC007561 RA
      atgggtggc agaagtaag aagatggat ttggtgcgg 
      actaacagt atttgtcct ccaccggaa accgttctt 
      ctagaaagc gtgaagaag aacgacaca gaatctatg 
      aaaacagta ataaaagca aacccatct attttgcaa 
      catctgtac ccctacaca tctcaaacc atcttatca 
      ctggcttgt tccgaacct ggcgttgct cctgacacc 
      gtcaaccaa ctgattgaa ttaggagca gacatcgag 
      ggaacccca cagtggaaa ccactgcac ctatcagcc 
      gacaatccc aacatccta attctagaa gtcgtcatc 
      aaacacctc aaaccaggc caaatcaat gaaaaatgg 
      aacggcaac accgcccta catacgctt atcaaagga 
      gaaaaaatc aaaaaggac gaagaaaat ttctgtcgg 
      cacgtggag cttctcttg cagtctgga attgatgtt 
      aatcagggt gacggcaag aatttaacc gcgattttt 
      tgggccgcc aagtacggt tataaaaaa attgtaaaa 
      gttatactt gaggagagt gtgcttcat gtcgatctg 
      gacacttgc agtttgcgc gataaaact gcaagggat 
      ttaataaat gagaaaggg ttgtacgag gggcctttg 
      ccggaacgt atcctgtat caagtcccc aaagacaaa 
      attttcggg ttgattaag gaaggaaaa gaggacgag 
      tttatctct tattttgag acattgtcc ttgaataat 
      ccctccgac tttgttaat gcagacgac ggtgcctca 
      acactgtta cagtacagt tgcgaaagg gggttagca 
      aaagtcgta gaatatctt ttagacaag ggggccaac 
      gtcaatctt gtcacaaaa aatcaaagg aagccgatt 
      gatattgtc gcagatatt ggctacttt gaaattttt 
      gaattgcta ttcaaatgc cctgactta gaattatcc 
      atagacaca ctttgtaac ctactaaaa cactcaaac 
      tctccaaaa tttggcaaa atcaatcac gaaaagtgt 
      tgcaaattg ctattagat aaactgggt aatagacgt 
      cctcctatt gatatcaat gaagtggac catttaaac 
      aacatccct cttcactac tccctccgg tattgcgat 
      acaacaacc acacaaaaa ttactgcaa cttggagct 
      tctctggct tataaaaac gaattcggt tctatgcca 
      atccaagac atcaaaccg gaagtgctc gagaaccat 
      ctcgacaac tgtgtcaca tttgatctc aaaaacgcc 
      gagaaacgc gattttgaa gtagtcttc gactaccag 
      acgctgcta cccccgaga aaacgcaag cgattcaaa 
      tacgaagag gtggaccct gaatttttg gccacaaac 
      aatgtaaaa cctgaaact gaagttata gcctacatg 
      agcaaagct ccagaattt cgagccttg ctcaagcac 
      ccccttatt gtcagtttc ctctttatg aaatggcac 
      caaatccgc ttactgttt tacacaaat ctggtgttc 
      tatatttgc tttgtgctg tctttagtt gtctacatt 
      tttacgcat tatgctaat ttcgaccgg acgcagtcg 
      gattattgt ctcattttt ggcaaattt tcgtggttc 
      actctaaat ttaacattt tgggttttg gtgttacgt 
      gaggttttc caagtcgcg gtggcgcct cgaaaatat 
      ttttgcaat ttcgaaaat ttggttgaa attatttta 
      attgtggtg actgggatg attttgtat attgactcg 
      cccactagc cacacaagg cgacagttg gcgtcagtg 
      gcgatatta ttggcagcg tttgagttg gttttgatg 
      gttggccaa catccgaaa ttatccacc aatgttgta 
      atgttgaaa acggtttcg gtcaatttt ttcaagttg 
      cttttgtgg tattccttg ctaattata gcgtttgct 
      ttgagtttt tatatctta tttgccaaa acagagatg 
      gctcaaagt gtgaatggg actgagacg ggggatgaa 
      gacgatgtc ttcaaaggg cccggaaag tcgctgttt 
      aaaactata gttatgttg accggtgag ttcgatgcc 
      gggtcaatc aatttccat acttaccct gtcactagt 
      aagataatt ttttcactt tttgtcttt atgatcact 
      attattttg ctcaatctg ttgaatggt ttagctgtc 
      agtgataca caaacgatt aagaacgac gcagagtta 
      gtgggccat atctcaagg gcccagcat atttattac 
      gtggaaagt atgttactt ggcaatatt ttaccgact 
      tctttcatt caagcagtg caaagattg ttctgttgc 
      tgtccctgt gactcggat acgacttac acgtttttt 
      aagccttta tcacgaaaa gtttgcctt tttacacaa 
      aattgccag ctgactgtt ttacctaac gagtacggt 
      aaaatatct tgtgaattg aattcatcc gctaaaaaa 
      cgaccagac ccattggta acttgcacc cgaaattgt 
      tctgaagct tatttagat aaaaacact attaggaga 
      atcgctgat atcgttaaa gcccgaaga gaacgtgaa 
      gagtatcct acagttact aatctaaag cagttatat 
      ggagaaatt gtgagcata aaagcgaag ctcgatcaa 
      attttggga agtctctcg acaaatcaa aacgcttta 
      tga
    mRNA sequence
    • >TC007561 RA
      tgacttccc acttgttgt gaaggtttt gtggtgtga 
      caactgcgg aaaatgttc tgaaatgtg attgtgaca 
      cgtaaataa gttggtttt cgtaaagaa aaaaaggag 
      acgagttgt gagcaatcg tgtgttcaa atttggcgg 
      aggaatgag tgcttcaac actccctaa ttgattgag 
      ggaaaaccc tccgaatta tcaaacatt aatattagt 
      tatcttcgg gtgtgctat tatattgtt gcatgtttt 
      tagaacgaa ctgtactca ttattgtgc tgatctaat 
      aaaacaagt ggttcattt agagatccg gtccagcga 
      ccaccgcct ctgtacatt taaaacatg ggtggcaga 
      agtaagaag atggatttg gtgcggact aacagtatt 
      tgtcctcca ccggaaacc gttcttcta gaaagcgtg 
      aagaagaac gacacagaa tctatgaaa acagtaata 
      aaagcaaac ccatctatt ttgcaacat ctgtacccc 
      tacacatct caaaccatc ttatcactg gcttgttcc 
      gaacctggc gttgctcct gacaccgtc aaccaactg 
      attgaatta ggagcagac atcgaggga accccacag 
      tggaaacca ctgcaccta tcagccgac aatcccaac 
      atcctaatt ctagaagtc gtcatcaaa cacctcaaa 
      ccaggccaa atcaatgaa aaatggaac ggcaacacc 
      gccctacat acgcttatc aaaggagaa aaaatcaaa 
      aaggacgaa gaaaatttc tgtcggcac gtggagctt 
      ctcttgcag tctggaatt gatgttaat cagggtgac 
      ggcaagaat ttaaccgcg attttttgg gccgccaag 
      tacggttat aaaaaaatt gtaaaagtt atacttgag 
      gagagtgtg cttcatgtc gatctggac acttgcagt 
      ttgcgcgat aaaactgca agggattta ataaatgag 
      aaagggttg tacgagggg cctttgccg gaacgtatc 
      ctgtatcaa gtccccaaa gacaaaatt ttcgggttg 
      attaaggaa ggaaaagag gacgagttt atctcttat 
      tttgagaca ttgtccttg aataatccc tccgacttt 
      gttaatgca gacgacggt gcctcaaca ctgttacag 
      tacagttgc gaaaggggg ttagcaaaa gtcgtagaa 
      tatctttta gacaagggg gccaacgtc aatcttgtc 
      acaaaaaat caaaggaag ccgattgat attgtcgca 
      gatattggc tactttgaa atttttgaa ttgctattc 
      aaatgccct gacttagaa ttatccata gacacactt 
      tgtaaccta ctaaaacac tcaaactct ccaaaattt 
      ggcaaaatc aatcacgaa aagtgttgc aaattgcta 
      ttagataaa ctgggtaat agacgtcct cctattgat 
      atcaatgaa gtggaccat ttaaacaac atccctctt 
      cactactcc ctccggtat tgcgataca acaaccaca 
      caaaaatta ctgcaactt ggagcttct ctggcttat 
      aaaaacgaa ttcggttct atgccaatc caagacatc 
      aaaccggaa gtgctcgag aaccatctc gacaactgt 
      gtcacattt gatctcaaa aacgccgag aaacgcgat 
      tttgaagta gtcttcgac taccagacg ctgctaccc 
      ccgagaaaa cgcaagcga ttcaaatac gaagaggtg 
      gaccctgaa tttttggcc acaaacaat gtaaaacct 
      gaaactgaa gttatagcc tacatgagc aaagctcca 
      gaatttcga gccttgctc aagcacccc cttattgtc 
      agtttcctc tttatgaaa tggcaccaa atccgctta 
      ctgttttac acaaatctg gtgttctat atttgcttt 
      gtgctgtct ttagttgtc tacattttt acgcattat 
      gctaatttc gaccggacg cagtcggat tattgtctc 
      atttttggc aaattttcg tggttcact ctaaattta 
      acattttgg gttttggtg ttacgtgag gttttccaa 
      gtcgcggtg gcgcctcga aaatatttt tgcaatttc 
      gaaaatttg gttgaaatt attttaatt gtggtgact 
      gggatgatt ttgtatatt gactcgccc actagccac 
      acaaggcga cagttggcg tcagtggcg atattattg 
      gcagcgttt gagttggtt ttgatggtt ggccaacat 
      ccgaaatta tccaccaat gttgtaatg ttgaaaacg 
      gtttcggtc aattttttc aagttgctt ttgtggtat 
      tccttgcta attatagcg tttgctttg agtttttat 
      atcttattt gccaaaaca gagatggct caaagtgtg 
      aatgggact gagacgggg gatgaagac gatgtcttc 
      aaagggccc ggaaagtcg ctgtttaaa actatagtt 
      atgttgacc ggtgagttc gatgccggg tcaatcaat 
      ttccatact taccctgtc actagtaag ataattttt 
      tcacttttt gtctttatg atcactatt attttgctc 
      aatctgttg aatggttta gctgtcagt gatacacaa 
      acgattaag aacgacgca gagttagtg ggccatatc 
      tcaagggcc cagcatatt tattacgtg gaaagtatg 
      ttacttggc aatatttta ccgacttct ttcattcaa 
      gcagtgcaa agattgttc tgttgctgt ccctgtgac 
      tcggatacg acttacacg ttttttaag cctttatca 
      cgaaaagtt tgccttttt acacaaaat tgccagctg 
      actgtttta cctaacgag tacggtaaa atatcttgt 
      gaattgaat tcatccgct aaaaaacga ccagaccca 
      ttggtaact tgcacccga aattgttct gaagcttat 
      ttagataaa aacactatt aggagaatc gctgatatc 
      gttaaagcc cgaagagaa cgtgaagag tatcctaca 
      gttactaat ctaaagcag ttatatgga gaaattgtg 
      agcataaaa gcgaagctc gatcaaatt ttgggaagt 
      ctctcgaca aatcaaaac gctttatga tttaatgtt 
      acgtatatt ttgttgtac tattgttgc catacttaa 
      tagagtaga tgtaattaa ccaacaggg tggtggaag 
      agcttctgg tgtgttata gatttctac cagtatttt 
      atgttttat agagtttac taacacgga aaagactat 
      tttatggtc cttgattta ttagtgcat attgtgtac 
      atatcttgt atttatttt taagccatt aattatgta 
      ttaaaattt ttacgtaaa taaatcatt atctataaa 
      ttcaagtct ttataattg tgtatttcg tacgtgttt 
      ctcaaattg aatgcgtaa caaaggtaa aaatgagaa 
      tacgtagtt tgctttatc tatatttta atcaaacgg 
      acaattctt tacaagaca ttcactgcg acatctccc 
      taattccct atcaaataa tttaaataa gtcacaaat 
      tttaatctc tttaacaat cacaccatt aacgatttt 
      acattcgta atatttctt gcaactggt ttcaaacgg 
      aaaccggcc aacttgcat acaattgaa ggaaccgtg 
      gtcaggtct acattgttt cgaaggaac acaaaactt 
      tctctttgc atcaaatgc gtgtcctgg aggacatct 
      gtattgttt caattcaaa atcgaattt aataaacct 
      ccaaataca cttgtaaaa cctgttaca gtcggttgg 
      atgaggaaa tatgcctgg tttatcaca aactaattg 
      tatttcgtt agggcactg gactgggtt tatagtgca 
      atgcttgtt gtaagaatc atactttgt gttaagtgg 
      gcaggaaat agggatgtc tataatttg gaaaatagc 
      atttatgca catgtgata tttactgca atcgaaggg 
      atcggggta ttttccaat acctggaca agtgaaaac 
      tggagatga ggttgtgat ttcactcga a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY