| General information | |||
| TC007560 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3021282..3024515 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007560 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01218 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC007560 RA
MSVIFLSKQ TKEEFKKFF SSFDTVLFD CDGVLWLDN EPISGSVPV VNRLRELGK RIFFVTNNS TKMRNEFAV KAKRMNFNI ETDEIISTA YLAAAYLKN MDFKQSVYV VGSRGITQE LDAVGIKHY GVGPDVLQN ALVHVIENF QMESDVGAV IVGYDEHFS YVKMMKAAS YLNNPNCLF IATNTDERF PMSTDLVIP GTGAIVSAV ETCAQRSPI VVGKPNPYI VDDLIKKYG IVPKRTLMI GDRVNTDIL LGTRCGFQT LLVLSGVTT LKEAVALKN SHKKEDKEM VADFYLEKL GDILPLLD
- >TC007560 RA
atgtctgtg atttttctt tcaaagcaa accaaagag gagtttaag aagtttttc agttctttc gacactgtt cttttcgac tgtgatggg gttttatgg cttgacaat gaaccgatt tctggttca gtcccggtt gtgaacaga ctccgcgaa ttgggtaaa agaatattc ttcgttaca aacaactcg acaaaaatg cgaaatgaa tttgccgtt aaggcgaaa cgtatgaat tttaacatt gaaacggac gaaattata tcaacggca tacttggca gcggcctat ttgaaaaac atggatttt aaacaatcc gtttacgtg gtgggctct cggggaatc acccaagag ctggacgct gtcggtatc aagcattac ggagttggg ccagacgtt ttgcaaaac gctctggtc catgtcatc gagaatttc caaatggaa agtgacgta ggcgccgtt atcgtcggc tatgacgag cacttctca tacgtgaaa atgatgaaa gcggcctct tatctaaac aatcctaac tgccttttt attgccact aacacagac gagcgattt ccgatgagt acggactta gtaatccca ggaactggg gccattgtc tcagctgtg gagacctgc gcccagcgc agccccatt gtcgtgggc aagcccaac ccatacatt gtggatgac ctcataaaa aaatacgga attgtgccc aaaagaaca ctcatgata ggcgatcgc gtcaacacc gatattttg ctgggaacg cggtgcggc tttcaaacc ttgttggtg ctatctgga gttacgacg ctcaaagaa gcagtcgct ttgaaaaat tcgcacaaa aaggaggac aaggaaatg gtcgctgat ttttacctg gagaagctg ggagatata ctgccactt ttagattga
- >TC007560 RA
aagcaaaga tgacaaaat ctgaaaatt ttattttat tcaaattga attgatgtt tgtgattcc cagggcatg agcagctgt ctttttgac aggtcacct aacctaaaa gtatttcaa aaatcctaa ccaaaaaaa gacaacgtg tgatctttc tttaatttt ttattaggc gtttttata ctaaaaata cagttgtga aaacagaat tatgttcca ctggattta ttgtacgtt tggatatgt atttatttt cgtcacatt aacatctaa ctaaaccac ttatttttc tcaaaattg ataaaaatc aaataaaat ccgagattc cgaatagca ataacatgt tctagtggt aattattta cttttagca aattattcc tcctcaagt tacaatctt ccgctttct attacctac ttttctcca gttgttcag tgactgtac aactgcaac aacatgcac ctacttttc aacataaat ctctgcaaa ctgtttttt gtgataata acatcgtct ttggtgata ctcggtgtt ttgataaat ttttttgag ccacacttg ccggctttt atcaaaaaa accttcaga tatggaaaa tttgcatct gatgaaaaa tcacttcta gtgcaaagt gtggaagtc ccaaaatcg tatttcagg catttttat gttgctgca gcattcatt ttttgccca atttttctg aaagaggtt gtggctgtt tatgtgttt aataaggga gttgatgat tggattgag actttgctc aatttttct tgtggatat ggcagacta tcaacagtt ccagcccat cgtgtccta attgcgtcc atattattg agcctacaa atatttcgc cgattttac gacacacat tttgtgtcg gtttttagc gaaaatgcg atgattaat ttaagccat tacctcatc ggaatggct ttttaccca gctgtgatg ttgattatc ctaagtgaa gccccgaag tttgcccaa aacggtata atagctccc ttcacgcga cacagtttt tatacctct aaattagta gaaaccaac actactact ttcaattta agcgagatt atgtttaat ttctttata attgttgtc tttttgtgg gcttggtat caccagcat gttgccact gtgatacta gccaatctg aggaaaaac aaaaaaggt gatttgaga acaaaacaa aaaatattt aatagttat gatgggttc taggtgacg ttgttgatc agggttaca gactccctg agggtgact ggttcaatt acttatctt cacctcact caacagcag aaataataa tgtatacag ccctgactt tgttattgg caaaaaata caagttggt tgtacgtct atgcttgga ttttaagca atcaagtaa ttataattt gttttatga ctgaaacta attcccaat ttgcagatt gaaacaatt cttttaagc cattggtgg tatttggat cgtttcaaa aattttccc ccacaaagg aaggccctc attccattt atttattaa ttattagtc aaggtgtat ttcatttgt tgcaaataa attattttt tatttaccc gtgtttgca tttaccgat acataaaaa tttaaaacg aggtcaata attacgtct tttgaaata atctcctaa tggcgggag aacagtggc attggattg gtcaaacaa cttatgttg ccatagaaa catttttcc atttttttg attgacgtt tacgttttc aggttaggt taaaagtca ccttcacgc aataatttg gtacaaatt cctagataa cagtgaatt ttttgcccc acattattt aaaaaaatt gtcggcttg aaagcccgt tcagtgaaa ctaatttca aacacacct gccaaaatg tctgtgatt tttctttca aagcaaacc aaagaggag tttaagaag tttttcagt tctttcgac actgttctt ttcgactgt gatggggtt ttatggctt gacaatgaa ccgatttct ggttcagtc ccggttgtg aacagactc cgcgaattg ggtaaaaga atattcttc gttacaaac aactcgaca aaaatgcga aatgaattt gccgttaag gcgaaacgt atgaatttt aacattgaa acggacgaa attatatca acggcatac ttggcagcg gcctatttg aaaaacatg gattttaaa caatccgtt tacgtggtg ggctctcgg ggaatcacc caagagctg gacgctgtc ggtatcaag cattacgga gttgggcca gacgttttg caaaacgct ctggtccat gtcatcgag aatttccaa atggaaagt gacgtaggc gccgttatc gtcggctat gacgagcac ttctcatac gtgaaaatg atgaaagcg gcctcttat ctaaacaat cctaactgc ctttttatt gccactaac acagacgag cgatttccg atgagtacg gacttagta atcccagga actggggcc attgtctca gctgtggag acctgcgcc cagcgcagc cccattgtc gtgggcaag cccaaccca tacattgtg gatgacctc ataaaaaaa tacggaatt gtgcccaaa agaacactc atgataggc gatcgcgtc aacaccgat attttgctg ggaacgcgg tgcggcttt caaaccttg ttggtgcta tctggagtt acgacgctc aaagaagca gtcgctttg aaaaattcg cacaaaaag gaggacaag gaaatggtc gctgatttt tacctggag aagctggga gatatactg ccactttta gattgaaat tttaggaca ttagaccaa ttcttaaat tatttattg ttagtaggt accgcatat ctacctcca tgcaacagt agataagaa aatgtctgt taactcaga ataaaaagt gtcttatgc c
