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Gene information
General information
TC004090 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451350.1: 3473..6853
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004090
      iBeetle screen iB_01217

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

      head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      iBeetle screen iB_06661

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004090 RA
        MDALQAQVA ELEQKYADL MAQAQARVQ ASDTPSKQA 
        PTSSIVFSN SIPIPEKFS FEKEDWQVW ITHYERFRT 
        ATKLDKSTQ AEQINSLLL HMGAKVTKF MEAKQKQET 
        DFPSYKALK EFFDNEFKD TPNTIYARA KFNRRDQRE 
        GEDAQTYIA DVISLAKTC NYRDLEEEL IRDRLVVGI 
        RDEKLSENL QMNDKLTLK TAIDKITQV ERIRTENRE 
        LRVREETEN SAGAKMESN EAEHDTNVN KVLTLLSDH 
        GLTLNKSKC EFKTNKTLY LGYLISENG VQADPKSVE 
        AINNYPAPS SVTEVRTFL GMVNYVSKF IVNTSEKTQ 
        SLRELLHKD KQFVWTDKH QADFEKLKS EIASSRVLA 
        KFSTDKKSR VSADASSFG LGAVLEQLQ SDGNWKPTY 
        FCSRTLEPC EQAYAQIEK EALAITWAC ERLENFLLG 
        AHFEIHTDH KPLTVILAT KELNKLSNR LQRFRMRLL 
        KFNYTIEYV PGKTFFVPD ALSRAPIQG RIRHQDPLL 
        EDVQNLYIN FVMREVVTE ICSTEEIKS YQNKDPTYS 
        KLKEYVKNG WPQKHKSDP LVSKFFSHQ QDLSIAHDM 
        VCYKDRVVI PGPLQKKCL YVLHDGHFG INKCLDRAK 
        ATVWWPGIT RELKEVVGK CEICIKLRR PTVDPMLPS 
        EVPSRPWQT IGADLAEYH ESTYLVVQD YYSKYPEIR 
        KLRTTKAKV VIETFKEMF ARHGIPEVV RSDNGKQFD 
        CHEYRTFSR KYGFKLITS SPHYQQSNG QAESAVKLL 
        KTILRKNED PYLALLTYR NTPTKCGLS PAQLLFGRN 
        LRDRLPRLP SGLKPATID HDTVRQTLI NNQAEQKSN 
        YDKRHRVTK EEKNLKEGD RVWIINMQK EGEVQRRCE 
        EPRSYLIET DGGCVRRNR RHLQPLPDR NNEPEHDPE 
        QPDESEEPK KRPIRRPKR YQDYYCY
      CDS sequence
      • >TC004090 RA
        atggatgca cttcaggca caagtggcc gagttggag 
        caaaaatat gcggatctc atggcacaa gcacaggcc 
        cgggttcag gcgagtgac acaccgtca aagcaggct 
        ccaacgagt agcatcgtc ttcagcaac tccattccg 
        attccggag aaattttca ttcgaaaaa gaagattgg 
        caagtgtgg attacacat tatgaacgt tttagaaca 
        gcaacaaaa ctagacaaa agcacacaa gcagagcaa 
        attaatagt ttattgttg catatgggg gcgaaagtt 
        acaaagttt atggaggct aagcagaaa caggaaacc 
        gattttcct agttacaaa gcgctgaaa gaattcttt 
        gataacgag ttcaaagac actcctaat acaatctac 
        gcacgagct aagtttaac agacgtgac caaagggaa 
        ggtgaggac gcccaaacg tacatcgct gacgtcata 
        agcttggcc aaaacatgc aattacaga gatttggag 
        gaggaactc ataagagat cgattagtt gttggaatt 
        cgtgatgaa aaattgtcg gaaaatcta caaatgaat 
        gacaaattg acacttaaa acagcaatt gacaaaata 
        actcaagtg gagaggatt agaaccgaa aacagagaa 
        ctaagagtg agagaggaa acggaaaac agcgcagga 
        gcaaagatg gaaagcaac gaagctgaa catgacaca 
        aacgtgaac aaagtgtta acactctta tcggatcac 
        ggcttgact ttaaacaaa tccaaatgt gaatttaaa 
        acgaacaag accttatat ttaggatac ttaatatcc 
        gaaaatggt gttcaagcc gatcccaaa agcgttgaa 
        gccattaat aattatccc gcgccatcg tcggtaacc 
        gaagttaga actttcctc ggcatggtt aactatgtc 
        tcaaaattt atcgttaat acttcggaa aaaacccaa 
        tcattacgt gaactactg cacaaggat aaacagttt 
        gtatggaca gataaacat caagcggat tttgaaaaa 
        cttaaaagt gaaatcgca tcatcgcgc gtgttagcc 
        aagtttagc acggacaaa aaatcgcga gtgtcggca 
        gacgcatcg tcttttggt ttaggtgcc gttttagaa 
        cagttacaa agtgacgga aattggaaa cctacttat 
        ttttgttcg cgtaccctc gaaccatgc gagcaagcc 
        tatgctcaa attgaaaag gaagctctc gccattaca 
        tgggcgtgt gaaagactc gaaaatttc ttactgggc 
        gctcatttt gaaattcat accgaccat aaaccgtta 
        acagtaatt ttggccacc aaagagctc aacaaacta 
        tccaacagg ctacaaaga tttcgcatg cggttattg 
        aaatttaac tatactata gaatacgtt ccaggtaaa 
        acgtttttc gttcccgat gcattatcc agagcacca 
        atccaaggc agaattcga caccaagac ccacttcta 
        gaagacgtt caaaatctc tacatcaat ttcgtgatg 
        cgtgaggtc gttacagaa atttgttca acggaagaa 
        atcaagtct taccaaaac aaggaccca acgtacagc 
        aaactcaaa gaatacgtc aaaaatggt tggccacag 
        aaacacaaa agcgacccg ttagtcagt aaattcttc 
        tctcatcaa caagactta agcatcgct catgacatg 
        gtttgctac aaagaccga gtcgtcatt cctggtcct 
        cttcagaag aagtgcctc tatgtgctc catgacgga 
        cacttcggt attaataaa tgtttggac cgggccaaa 
        gctactgtg tggtggcca ggtatcaca agagagctt 
        aaagaagtc gtcgggaaa tgtgaaatt tgcatcaaa 
        ctacgcaga ccaacggta gatcccatg ttaccatcc 
        gaagttcca tctagaccg tggcaaaca attggtgcc 
        gacctcgca gaatatcat gagtcaact tacttagtg 
        gtacaggac tattattcc aagtatccg gaaatcaga 
        aaacttcgc accaccaaa gccaaagtt gttatcgag 
        accttcaaa gaaatgttt gctagacat ggtataccc 
        gaagttgtc cgctccgac aatggcaaa caatttgat 
        tgtcacgaa tacagaact ttcagcaga aaatatgga 
        tttaagtta attaccagt agtccccat taccaacag 
        tcaaatgga caagccgaa agtgctgtg aagctgctc 
        aaaacgata ttgaggaaa aatgaagat ccatatctt 
        gcacttctt acctaccgc aatacacct actaaatgt 
        ggattgtca cctgcccaa ctgctcttt ggcagaaac 
        cttcgagac agactgcca aggttacct tcagggtta 
        aaacctgca actatcgac catgacact gtcagacaa 
        acgttaatc aacaatcaa gcagaacag aagtccaac 
        tacgacaaa cgacaccga gtaacaaaa gaagagaaa 
        aatctaaaa gaaggtgat cgcgtatgg atcataaat 
        atgcaaaag gagggagaa gtacaacgc agatgtgaa 
        gaaccaagg tcatatttg atcgagact gacggcggt 
        tgcgtcaga cgaaatcga agacatctt cagccctta 
        ccagacaga aataatgaa cccgaacat gacccagaa 
        caaccagac gaatcagag gaaccaaag aaaagacca 
        atcagaaga cccaaaaga taccaagat tattattgt 
        tattga
      mRNA sequence
      • >TC004090 RA
        attgcactt cacgtcaca tgtaaaaat gacttgaag 
        taatttaaa atataggtc atggttctt atcttctcg 
        tcttcttgt taggttggc aactctcac gtgggcgac 
        acgggagtt ggagggggg ttttttggc agagtgagt 
        tagttaggt tatgtcgca gaaggggcc tggacgcac 
        cctcaatgc tccgggata ttagtcaat aaacagtta 
        taaggttga atggtctat ttacaacta acctaacat 
        ggtgtcagt gttttaaaa tccgaatcc cgctcgtgt 
        tacaaattg tgagtgtaa aaaccacca caactatca 
        cgatggatg cacttcagg cacaagtgg ccgagttgg 
        agcaaaaat atgcggatc tcatggcac aagcacagg 
        cccgggttc aggcgagtg acacaccgt caaagcagg 
        ctccaacga gtagcatcg tcttcagca actccattc 
        cgattccgg agaaatttt cattcgaaa aagaagatt 
        ggcaagtgt ggattacac attatgaac gttttagaa 
        cagcaacaa aactagaca aaagcacac aagcagagc 
        aaattaata gtttattgt tgcatatgg gggcgaaag 
        ttacaaagt ttatggagg ctaagcaga aacaggaaa 
        ccgattttc ctagttaca aagcgctga aagaattct 
        ttgataacg agttcaaag acactccta atacaatct 
        acgcacgag ctaagttta acagacgtg accaaaggg 
        aaggtgagg acgcccaaa cgtacatcg ctgacgtca 
        taagcttgg ccaaaacat gcaattaca gagatttgg 
        aggaggaac tcataagag atcgattag ttgttggaa 
        ttcgtgatg aaaaattgt cggaaaatc tacaaatga 
        atgacaaat tgacactta aaacagcaa ttgacaaaa 
        taactcaag tggagagga ttagaaccg aaaacagag 
        aactaagag tgagagagg aaacggaaa acagcgcag 
        gagcaaaga tggaaagca acgaagctg aacatgaca 
        caaacgtga acaaagtgt taacactct tatcggatc 
        acggcttga ctttaaaca aatccaaat gtgaattta 
        aaacgaaca agaccttat atttaggat acttaatat 
        ccgaaaatg gtgttcaag ccgatccca aaagcgttg 
        aagccatta ataattatc ccgcgccat cgtcggtaa 
        ccgaagtta gaactttcc tcggcatgg ttaactatg 
        tctcaaaat ttatcgtta atacttcgg aaaaaaccc 
        aatcattac gtgaactac tgcacaagg ataaacagt 
        ttgtatgga cagataaac atcaagcgg attttgaaa 
        aacttaaaa gtgaaatcg catcatcgc gcgtgttag 
        ccaagttta gcacggaca aaaaatcgc gagtgtcgg 
        cagacgcat cgtcttttg gtttaggtg ccgttttag 
        aacagttac aaagtgacg gaaattgga aacctactt 
        atttttgtt cgcgtaccc tcgaaccat gcgagcaag 
        cctatgctc aaattgaaa aggaagctc tcgccatta 
        catgggcgt gtgaaagac tcgaaaatt tcttactgg 
        gcgctcatt ttgaaattc ataccgacc ataaaccgt 
        taacagtaa ttttggcca ccaaagagc tcaacaaac 
        tatccaaca ggctacaaa gatttcgca tgcggttat 
        tgaaattta actatacta tagaatacg ttccaggta 
        aaacgtttt tcgttcccg atgcattat ccagagcac 
        caatccaag gcagaattc gacaccaag acccacttc 
        tagaagacg ttcaaaatc tctacatca atttcgtga 
        tgcgtgagg tcgttacag aaatttgtt caacggaag 
        aaatcaagt cttaccaaa acaaggacc caacgtaca 
        gcaaactca aagaatacg tcaaaaatg gttggccac 
        agaaacaca aaagcgacc cgttagtca gtaaattct 
        tctctcatc aacaagact taagcatcg ctcatgaca 
        tggtttgct acaaagacc gagtcgtca ttcctggtc 
        ctcttcaga agaagtgcc tctatgtgc tccatgacg 
        gacacttcg gtattaata aatgtttgg accgggcca 
        aagctactg tgtggtggc caggtatca caagagagc 
        ttaaagaag tcgtcggga aatgtgaaa tttgcatca 
        aactacgca gaccaacgg tagatccca tgttaccat 
        ccgaagttc catctagac cgtggcaaa caattggtg 
        ccgacctcg cagaatatc atgagtcaa cttacttag 
        tggtacagg actattatt ccaagtatc cggaaatca 
        gaaaacttc gcaccacca aagccaaag ttgttatcg 
        agaccttca aagaaatgt ttgctagac atggtatac 
        ccgaagttg tccgctccg acaatggca aacaatttg 
        attgtcacg aatacagaa ctttcagca gaaaatatg 
        gatttaagt taattacca gtagtcccc attaccaac 
        agtcaaatg gacaagccg aaagtgctg tgaagctgc 
        tcaaaacga tattgagga aaaatgaag atccatatc 
        ttgcacttc ttacctacc gcaatacac ctactaaat 
        gtggattgt cacctgccc aactgctct ttggcagaa 
        accttcgag acagactgc caaggttac cttcagggt 
        taaaacctg caactatcg accatgaca ctgtcagac 
        aaacgttaa tcaacaatc aagcagaac agaagtcca 
        actacgaca aacgacacc gagtaacaa aagaagaga 
        aaaatctaa aagaaggtg atcgcgtat ggatcataa 
        atatgcaaa aggagggag aagtacaac gcagatgtg 
        aagaaccaa ggtcatatt tgatcgaga ctgacggcg 
        gttgcgtca gacgaaatc gaagacatc ttcagccct 
        taccagaca gaaataatg aacccgaac atgacccag 
        aacaaccag acgaatcag aggaaccaa agaaaagac 
        caatcagaa gacccaaaa gataccaag attattatt 
        gttattgaa atatgtata ttttgatac ctcaggtag 
        ttagtttca tatttagct tagttttgt atttaaaag 
        gggggatgt taggttggc aactctcac gtgggcgac 
        acgggagtt ggagggggg ttttttggc agagtgagt 
        tagttaggt tatgtcgca gaaggggcc tggacgcac 
        cctcaatgc tccgggata ttagtcaat aaacagtca 
        ttgttttt
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