Status
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Gene information
General information
TC007535 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3282831..3287658
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007535
    iBeetle screen iB_01215

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007535 RA
      MDGVVFSIP TNRADLLNE SERKYTVKN VVVSSEILK 
      KLEEARTGL LEEGPYFIL DNFDTYYSI LHQENKLSI 
      DVIQKGFNS LLKAVKQLN DTILFLVED PASLSEESK 
      SKMANVLKM LTYIFVQFV LYLEEKNKR SGDLLSKGK 
      KEKKHDNFG VNKSEVVKA LNNIIQRPI NVFWQPPIV 
      EHEYIELLS ELCFGLLQN PSIKQEKVV IMDIFGVFG 
      KLVKSYDFG ETFVARMIR NVCMYEHVV HCVPEGIKM 
      MVEQFSCQN LVHHLIIEA TEWQATETV QDVQGTRCC 
      SIFLTELAK TVPNLVFPE VTDLSLYLA HQSVPLRTA 
      VLNVITEII LHVLTRHDL NQEEKDSRA TCFDILTEH 
      ILEYSPIIR AKVIQNLNR LQKENAIPL VIQNDVLSK 
      VVRHLSDKS ATVRKCAVS CVTTFLECN PFGAKLQLS 
      EAEEQLAQK IELLRKMEA AYQEQLVAK VQELEAGWL 
      TIENDLLVV VTEELKTDE DEDEDERES VESEQFAEQ 
      IQSLLHEKK YAEAFRMCR RFTNNIPES RNVANEDQA 
      KFYMNILRS FYINTVKFL TEMRTTAGM SQEDVEKIK 
      ALEGVIKYF EDMVKFLRT IHSVVEPMK MVLLSDTMS 
      DTHEAIKFF VSAYHFKID NANEGIIEM LKMMRTNHE 
      ERKSAVTEA LRTIYLVTD TKSMEEHTT TILNRLFAL 
      LRNLTYDRL EDFQDVVSE WTAKGILDN AVINRCWQY 
      YTQHEHVSA EDARAAAVL LRMAAMGRK TIITKNLNV 
      VATMAFSQQ YKSDMLFLA NSFRLLSVA GLDKIDVQS 
      EETPFRIKK TEDLFHQLS DNLIENFFN DLPYYSEAM 
      QACTDFTFR MCSKPMEII ENIIKEIDE RFRQRPQTI 
      VLSIRFLEM LGCIALKFL EYLDNGVHR EMKRRTYLR 
      QEREKAKAN KKNKRKSKR QSVLDTSNA SDVSLSGSI 
      VLGAEAEDV DTEYIIEAL ENDVVSKKG VLGQYAPFV 
      LKFCQRPDI YSDVLLQTA AVTTLSRYM LISSKFCAE 
      NIRLLFTIL AKTSHPDIR STISMHCVD LLTRFPNII 
      DPWSPRLSE MLKDSVAEV RKNAFFSLS NLILRDMIR 
      ASSRISEMA VCLIDPDPE VSQLCRSFF TKIAQKDNN 
      NIVSLIPDI FSYLVKQEE ITEADLRYV IKFLFDLID 
      VPKRPENLV DRLCHKYTP DENVRLNRN ITYCLSLIN 
      YNDKALQKL RDMFPLYRH NVTDEEVYS TFKQILNEC 
      GKAKVGRVD LKPIVTEIE NCIASVFEI HEGGPPVKP 
      PTVAKSGRT GRKKKGPAK RARKVQSDS EEEDSGEDA 
      LPKSTPSRN KTSRNRRNS
    CDS sequence
    • >TC007535 RA
      atggacggg gttgtcttt tcaatcccc acaaatcga 
      gccgatctt ctgaacgaa agtgaaaga aaatacact 
      gtgaagaat gtcgtggtt tcttctgaa atcctcaaa 
      aaacttgaa gaggctcga acagggctt ctcgaagag 
      ggcccgtac tttattttg gacaatttc gacacctac 
      tattccatt ttgcatcaa gaaaataag ctatccatc 
      gatgtaatt cagaaaggg ttcaacagt ttactgaag 
      gcggtcaag cagctcaac gacacgatc ctcttcctg 
      gtcgaagac ccggccagt ctgagtgag gaatcaaag 
      tcgaaaatg gccaacgtt ttaaaaatg ctgacgtac 
      atctttgtc cagttcgtc ctgtacttg gaggaaaaa 
      aacaaacgt tctggcgac ttactaagc aagggtaaa 
      aaggagaaa aagcacgat aacttcggc gtaaacaaa 
      tccgaggtt gtgaaagca ttgaataac ataattcaa 
      agaccgata aacgtcttc tggcaaccg ccgattgtg 
      gaacacgag tacatcgag ttgttgagc gaattatgt 
      ttcggtctt ttacaaaac ccgtccata aaacaggaa 
      aaagtggtg attatggac atattcggc gtgtttggt 
      aaattggta aagtcgtac gacttcggc gagactttc 
      gttgcgcga atgatacga aacgtgtgc atgtacgaa 
      catgtagta cactgtgtt cccgaggga atcaaaatg 
      atggtggag cagtttagt tgccaaaac ttggttcat 
      cacctcatc attgaggcg acggaatgg caagcgact 
      gagacagtc caagacgtt caagggaca cggtgctgt 
      tccattttt ctgacagag ttggccaaa acggtcccc 
      aacttagtc ttccctgaa gtaaccgat ctcagtctc 
      tatctagca caccaatct gttccgtta cgtacagcc 
      gttcttaac gtcattact gaaattatt ttacacgtt 
      ttaacaaga cacgactta aatcaggaa gagaaagac 
      tcgcgcgct acgtgcttc gatatttta acggaacac 
      attttggaa tattcgccc ataattcgg gcgaaagtt 
      atacaaaac ttgaacagg ctccagaag gaaaatgcc 
      atacctttg gtcattcag aacgatgtt ttaagtaaa 
      gtggtgcga catttgtcg gataaatcg gcaacggtg 
      aggaaatgt gcagtcagc tgtgttacc acctttctg 
      gagtgtaat ccattcggg gcaaaactc caactatcc 
      gaagctgag gaacaacta gcgcaaaaa atcgaattg 
      ttgcgaaaa atggaagca gcttaccag gaacagttg 
      gtggcaaaa gtgcaagaa ctggaagct gggtggtta 
      acgattgag aacgatttg ttagttgtg gttacggaa 
      gaattaaag acagacgag gatgaagat gaggacgaa 
      agagagtca gtcgagagt gaacaattt gcggagcaa 
      attcaatct ctcctgcat gaaaaaaaa tacgccgaa 
      gcgttccga atgtgtcgc cgcttcaca aacaacatt 
      ccggagtct cgaaatgtt gcgaacgaa gatcaagcc 
      aaattctac atgaatatt ctgcgctcg ttttatatc 
      aacactgtt aaattttta acagaaatg cgaaccacg 
      gccggtatg agccaggaa gatgttgag aaaattaag 
      gcactggaa ggggtcatt aaatatttc gaagacatg 
      gtcaagttt ttacgcaca attcattcg gtcgtggaa 
      ccaatgaag atggtgctg ctctcagac acgatgagt 
      gacacccac gaagcaatc aagtttttt gtctcggcc 
      tatcatttt aaaatcgat aacgccaac gaggggatt 
      atagaaatg ttaaaaatg atgcgaact aaccacgaa 
      gaacgcaaa agtgcggtc actgaggcg ctccgaacc 
      atttatctg gtgactgat acgaagagt atggaggag 
      cacacgacg actatttta aaccgcctg tttgcacta 
      ttgaggaat ttaacctac gaccgcttg gaggacttt 
      caagacgtt gtctcggaa tggaccgcc aagggcatt 
      ctggacaac gctgttatt aacaggtgt tggcagtat 
      tatacccag cacgagcac gtttccgcc gaagatgcc 
      cgagcagcg gccgtgttg ctccgaatg gctgcgatg 
      ggcagaaag acgataata acaaaaaac ctgaatgtt 
      gttgcaaca atggcgttc agccaacaa tacaagagc 
      gatatgttg tttttggcg aattcattt cggctcctg 
      tcggtggca ggtcttgac aaaatcgat gttcaaagt 
      gaggaaaca ccgttcaga atcaagaaa acggaggat 
      ttgtttcac cagttatcg gataactta atcgagaat 
      tttttcaat gatttgccg tactattcc gaagctatg 
      caagcctgt accgacttt accttccga atgtgctcg 
      aaaccgatg gaaattatc gagaatatt attaaagaa 
      attgatgag cggttcaga cagcggccg caaacaatc 
      gttttaagt attaggttt cttgaaatg ttggggtgt 
      atagcgttg aaatttttg gagtatttg gacaacggc 
      gtgcaccgg gaaatgaaa aggaggact tacctgaga 
      caagagcgc gaaaaggcc aaagccaat aaaaagaat 
      aagaggaaa agtaaaaga cagagtgtg cttgacacc 
      tctaatgca agcgatgtc agtctgagt ggaagtatc 
      gtgctgggg gctgaagct gaagacgtc gacactgaa 
      tatataata gaggcttta gaaaacgac gtcgtctcc 
      aagaaaggt gttttagga caatatgcc ccttttgta 
      ctaaagttc tgtcaaaga ccggacatt tacagcgat 
      gttttgctg caaacagct gcagtaaca acgctttcc 
      aggtacatg ttgatttca agcaagttc tgtgccgaa 
      aacatccgg ctgttgttc acgattttg gcaaaaacg 
      agtcatcct gatatcaga tcgacgatt tcgatgcat 
      tgtgtcgat ttgttgact agatttccg aatataatc 
      gacccatgg agtccgcga ctttccgaa atgttaaaa 
      gattcagta gccgaagtg cggaaaaat gcatttttc 
      tcactgtcg aatttaatt ttgagagat atgatcaga 
      gcatcgagt cgcatatcg gagatggcg gtttgtttg 
      attgatccc gatccggaa gtcagccaa ctttgtaga 
      tcctttttc acaaaaatt gctcaaaaa gacaacaat 
      aacatcgtt agtctcatt cctgatatc ttcagttat 
      ttggtgaaa caagaggag atcactgag gctgatcta 
      agatacgtc ataaagttc ttgtttgat ttaatcgat 
      gtgccaaaa cggccggaa aacttggtt gaccgtctg 
      tgccacaaa tacacacca gacgaaaac gtcagactc 
      aaccggaat ataacttat tgcctgtct ttaataaat 
      tacaacgac aaggccttg caaaaatta cgcgatatg 
      ttcccgctt taccggcac aatgtcaca gatgaggaa 
      gtttactcg acctttaaa cagatattg aatgagtgt 
      ggcaaggca aaagtcggt cgagttgat ttaaaacca 
      attgttacg gaaatcgag aattgtatt gcgtccgtt 
      tttgaaatt cacgaaggc gggccccct gttaaacca 
      ccaacggtt gcaaaatcg ggaagaacg ggacgaaag 
      aaaaagggg ccagcaaaa agggcgaga aaagtgcag 
      tcagatagc gaggaagag gactcaggg gaggatgcg 
      ctacctaaa agtacacct agcagaaat aagacgtca 
      agaaacaga agaaattct taa
    mRNA sequence
    • >TC007535 RA
      gacgaggtc gacatctgg caacgtgta tttatgttt 
      ttcaaaccg tcagcgtca gtggttgtc attgtgttt 
      ttagctata agtttgtgt atattttat catttttta 
      aataattac tgacataaa aaatggacg gggttgtct 
      tttcaatcc ccacaaatc gagccgatc ttctgaacg 
      aaagtgaaa gaaaataca ctgtgaaga atgtcgtgg 
      tttcttctg aaatcctca aaaaacttg aagaggctc 
      gaacagggc ttctcgaag agggcccgt actttattt 
      tggacaatt tcgacacct actattcca ttttgcatc 
      aagaaaata agctatcca tcgatgtaa ttcagaaag 
      ggttcaaca gtttactga aggcggtca agcagctca 
      acgacacga tcctcttcc tggtcgaag acccggcca 
      gtctgagtg aggaatcaa agtcgaaaa tggccaacg 
      ttttaaaaa tgctgacgt acatctttg tccagttcg 
      tcctgtact tggaggaaa aaaacaaac gttctggcg 
      acttactaa gcaagggta aaaaggaga aaaagcacg 
      ataacttcg gcgtaaaca aatccgagg ttgtgaaag 
      cattgaata acataattc aaagaccga taaacgtct 
      tctggcaac cgccgattg tggaacacg agtacatcg 
      agttgttga gcgaattat gtttcggtc ttttacaaa 
      acccgtcca taaaacagg aaaaagtgg tgattatgg 
      acatattcg gcgtgtttg gtaaattgg taaagtcgt 
      acgacttcg gcgagactt tcgttgcgc gaatgatac 
      gaaacgtgt gcatgtacg aacatgtag tacactgtg 
      ttcccgagg gaatcaaaa tgatggtgg agcagttta 
      gttgccaaa acttggttc atcacctca tcattgagg 
      cgacggaat ggcaagcga ctgagacag tccaagacg 
      ttcaaggga cacggtgct gttccattt ttctgacag 
      agttggcca aaacggtcc ccaacttag tcttccctg 
      aagtaaccg atctcagtc tctatctag cacaccaat 
      ctgttccgt tacgtacag ccgttctta acgtcatta 
      ctgaaatta ttttacacg ttttaacaa gacacgact 
      taaatcagg aagagaaag actcgcgcg ctacgtgct 
      tcgatattt taacggaac acattttgg aatattcgc 
      ccataattc gggcgaaag ttatacaaa acttgaaca 
      ggctccaga aggaaaatg ccatacctt tggtcattc 
      agaacgatg ttttaagta aagtggtgc gacatttgt 
      cggataaat cggcaacgg tgaggaaat gtgcagtca 
      gctgtgtta ccacctttc tggagtgta atccattcg 
      gggcaaaac tccaactat ccgaagctg aggaacaac 
      tagcgcaaa aaatcgaat tgttgcgaa aaatggaag 
      cagcttacc aggaacagt tggtggcaa aagtgcaag 
      aactggaag ctgggtggt taacgattg agaacgatt 
      tgttagttg tggttacgg aagaattaa agacagacg 
      aggatgaag atgaggacg aaagagagt cagtcgaga 
      gtgaacaat ttgcggagc aaattcaat ctctcctgc 
      atgaaaaaa aatacgccg aagcgttcc gaatgtgtc 
      gccgcttca caaacaaca ttccggagt ctcgaaatg 
      ttgcgaacg aagatcaag ccaaattct acatgaata 
      ttctgcgct cgttttata tcaacactg ttaaatttt 
      taacagaaa tgcgaacca cggccggta tgagccagg 
      aagatgttg agaaaatta aggcactgg aaggggtca 
      ttaaatatt tcgaagaca tggtcaagt ttttacgca 
      caattcatt cggtcgtgg aaccaatga agatggtgc 
      tgctctcag acacgatga gtgacaccc acgaagcaa 
      tcaagtttt ttgtctcgg cctatcatt ttaaaatcg 
      ataacgcca acgagggga ttatagaaa tgttaaaaa 
      tgatgcgaa ctaaccacg aagaacgca aaagtgcgg 
      tcactgagg cgctccgaa ccatttatc tggtgactg 
      atacgaaga gtatggagg agcacacga cgactattt 
      taaaccgcc tgtttgcac tattgagga atttaacct 
      acgaccgct tggaggact ttcaagacg ttgtctcgg 
      aatggaccg ccaagggca ttctggaca acgctgtta 
      ttaacaggt gttggcagt attataccc agcacgagc 
      acgtttccg ccgaagatg cccgagcag cggccgtgt 
      tgctccgaa tggctgcga tgggcagaa agacgataa 
      taacaaaaa acctgaatg ttgttgcaa caatggcgt 
      tcagccaac aatacaaga gcgatatgt tgtttttgg 
      cgaattcat ttcggctcc tgtcggtgg caggtcttg 
      acaaaatcg atgttcaaa gtgaggaaa caccgttca 
      gaatcaaga aaacggagg atttgtttc accagttat 
      cggataact taatcgaga attttttca atgatttgc 
      cgtactatt ccgaagcta tgcaagcct gtaccgact 
      ttaccttcc gaatgtgct cgaaaccga tggaaatta 
      tcgagaata ttattaaag aaattgatg agcggttca 
      gacagcggc cgcaaacaa tcgttttaa gtattaggt 
      ttcttgaaa tgttggggt gtatagcgt tgaaatttt 
      tggagtatt tggacaacg gcgtgcacc gggaaatga 
      aaaggagga cttacctga gacaagagc gcgaaaagg 
      ccaaagcca ataaaaaga ataagagga aaagtaaaa 
      gacagagtg tgcttgaca cctctaatg caagcgatg 
      tcagtctga gtggaagta tcgtgctgg gggctgaag 
      ctgaagacg tcgacactg aatatataa tagaggctt 
      tagaaaacg acgtcgtct ccaagaaag gtgttttag 
      gacaatatg ccccttttg tactaaagt tctgtcaaa 
      gaccggaca tttacagcg atgttttgc tgcaaacag 
      ctgcagtaa caacgcttt ccaggtaca tgttgattt 
      caagcaagt tctgtgccg aaaacatcc ggctgttgt 
      tcacgattt tggcaaaaa cgagtcatc ctgatatca 
      gatcgacga tttcgatgc attgtgtcg atttgttga 
      ctagatttc cgaatataa tcgacccat ggagtccgc 
      gactttccg aaatgttaa aagattcag tagccgaag 
      tgcggaaaa atgcatttt tctcactgt cgaatttaa 
      ttttgagag atatgatca gagcatcga gtcgcatat 
      cggagatgg cggtttgtt tgattgatc ccgatccgg 
      aagtcagcc aactttgta gatcctttt tcacaaaaa 
      ttgctcaaa aagacaaca ataacatcg ttagtctca 
      ttcctgata tcttcagtt atttggtga aacaagagg 
      agatcactg aggctgatc taagatacg tcataaagt 
      tcttgtttg atttaatcg atgtgccaa aacggccgg 
      aaaacttgg ttgaccgtc tgtgccaca aatacacac 
      cagacgaaa acgtcagac tcaaccgga atataactt 
      attgcctgt ctttaataa attacaacg acaaggcct 
      tgcaaaaat tacgcgata tgttcccgc tttaccggc 
      acaatgtca cagatgagg aagtttact cgaccttta 
      aacagatat tgaatgagt gtggcaagg caaaagtcg 
      gtcgagttg atttaaaac caattgtta cggaaatcg 
      agaattgta ttgcgtccg tttttgaaa ttcacgaag 
      gcgggcccc ctgttaaac caccaacgg ttgcaaaat 
      cgggaagaa cgggacgaa agaaaaagg ggccagcaa 
      aaagggcga gaaaagtgc agtcagata gcgaggaag 
      aggactcag gggaggatg cgctaccta aaagtacac 
      ctagcagaa ataagacgt caagaaaca gaagaaatt 
      cttaaaaca gacagttgt attattgtt actttcaat 
      aaagttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY