| General information | |||
| TC007535 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3282831..3287658 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007535 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01215 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC007535 RA
MDGVVFSIP TNRADLLNE SERKYTVKN VVVSSEILK KLEEARTGL LEEGPYFIL DNFDTYYSI LHQENKLSI DVIQKGFNS LLKAVKQLN DTILFLVED PASLSEESK SKMANVLKM LTYIFVQFV LYLEEKNKR SGDLLSKGK KEKKHDNFG VNKSEVVKA LNNIIQRPI NVFWQPPIV EHEYIELLS ELCFGLLQN PSIKQEKVV IMDIFGVFG KLVKSYDFG ETFVARMIR NVCMYEHVV HCVPEGIKM MVEQFSCQN LVHHLIIEA TEWQATETV QDVQGTRCC SIFLTELAK TVPNLVFPE VTDLSLYLA HQSVPLRTA VLNVITEII LHVLTRHDL NQEEKDSRA TCFDILTEH ILEYSPIIR AKVIQNLNR LQKENAIPL VIQNDVLSK VVRHLSDKS ATVRKCAVS CVTTFLECN PFGAKLQLS EAEEQLAQK IELLRKMEA AYQEQLVAK VQELEAGWL TIENDLLVV VTEELKTDE DEDEDERES VESEQFAEQ IQSLLHEKK YAEAFRMCR RFTNNIPES RNVANEDQA KFYMNILRS FYINTVKFL TEMRTTAGM SQEDVEKIK ALEGVIKYF EDMVKFLRT IHSVVEPMK MVLLSDTMS DTHEAIKFF VSAYHFKID NANEGIIEM LKMMRTNHE ERKSAVTEA LRTIYLVTD TKSMEEHTT TILNRLFAL LRNLTYDRL EDFQDVVSE WTAKGILDN AVINRCWQY YTQHEHVSA EDARAAAVL LRMAAMGRK TIITKNLNV VATMAFSQQ YKSDMLFLA NSFRLLSVA GLDKIDVQS EETPFRIKK TEDLFHQLS DNLIENFFN DLPYYSEAM QACTDFTFR MCSKPMEII ENIIKEIDE RFRQRPQTI VLSIRFLEM LGCIALKFL EYLDNGVHR EMKRRTYLR QEREKAKAN KKNKRKSKR QSVLDTSNA SDVSLSGSI VLGAEAEDV DTEYIIEAL ENDVVSKKG VLGQYAPFV LKFCQRPDI YSDVLLQTA AVTTLSRYM LISSKFCAE NIRLLFTIL AKTSHPDIR STISMHCVD LLTRFPNII DPWSPRLSE MLKDSVAEV RKNAFFSLS NLILRDMIR ASSRISEMA VCLIDPDPE VSQLCRSFF TKIAQKDNN NIVSLIPDI FSYLVKQEE ITEADLRYV IKFLFDLID VPKRPENLV DRLCHKYTP DENVRLNRN ITYCLSLIN YNDKALQKL RDMFPLYRH NVTDEEVYS TFKQILNEC GKAKVGRVD LKPIVTEIE NCIASVFEI HEGGPPVKP PTVAKSGRT GRKKKGPAK RARKVQSDS EEEDSGEDA LPKSTPSRN KTSRNRRNS
- >TC007535 RA
atggacggg gttgtcttt tcaatcccc acaaatcga gccgatctt ctgaacgaa agtgaaaga aaatacact gtgaagaat gtcgtggtt tcttctgaa atcctcaaa aaacttgaa gaggctcga acagggctt ctcgaagag ggcccgtac tttattttg gacaatttc gacacctac tattccatt ttgcatcaa gaaaataag ctatccatc gatgtaatt cagaaaggg ttcaacagt ttactgaag gcggtcaag cagctcaac gacacgatc ctcttcctg gtcgaagac ccggccagt ctgagtgag gaatcaaag tcgaaaatg gccaacgtt ttaaaaatg ctgacgtac atctttgtc cagttcgtc ctgtacttg gaggaaaaa aacaaacgt tctggcgac ttactaagc aagggtaaa aaggagaaa aagcacgat aacttcggc gtaaacaaa tccgaggtt gtgaaagca ttgaataac ataattcaa agaccgata aacgtcttc tggcaaccg ccgattgtg gaacacgag tacatcgag ttgttgagc gaattatgt ttcggtctt ttacaaaac ccgtccata aaacaggaa aaagtggtg attatggac atattcggc gtgtttggt aaattggta aagtcgtac gacttcggc gagactttc gttgcgcga atgatacga aacgtgtgc atgtacgaa catgtagta cactgtgtt cccgaggga atcaaaatg atggtggag cagtttagt tgccaaaac ttggttcat cacctcatc attgaggcg acggaatgg caagcgact gagacagtc caagacgtt caagggaca cggtgctgt tccattttt ctgacagag ttggccaaa acggtcccc aacttagtc ttccctgaa gtaaccgat ctcagtctc tatctagca caccaatct gttccgtta cgtacagcc gttcttaac gtcattact gaaattatt ttacacgtt ttaacaaga cacgactta aatcaggaa gagaaagac tcgcgcgct acgtgcttc gatatttta acggaacac attttggaa tattcgccc ataattcgg gcgaaagtt atacaaaac ttgaacagg ctccagaag gaaaatgcc atacctttg gtcattcag aacgatgtt ttaagtaaa gtggtgcga catttgtcg gataaatcg gcaacggtg aggaaatgt gcagtcagc tgtgttacc acctttctg gagtgtaat ccattcggg gcaaaactc caactatcc gaagctgag gaacaacta gcgcaaaaa atcgaattg ttgcgaaaa atggaagca gcttaccag gaacagttg gtggcaaaa gtgcaagaa ctggaagct gggtggtta acgattgag aacgatttg ttagttgtg gttacggaa gaattaaag acagacgag gatgaagat gaggacgaa agagagtca gtcgagagt gaacaattt gcggagcaa attcaatct ctcctgcat gaaaaaaaa tacgccgaa gcgttccga atgtgtcgc cgcttcaca aacaacatt ccggagtct cgaaatgtt gcgaacgaa gatcaagcc aaattctac atgaatatt ctgcgctcg ttttatatc aacactgtt aaattttta acagaaatg cgaaccacg gccggtatg agccaggaa gatgttgag aaaattaag gcactggaa ggggtcatt aaatatttc gaagacatg gtcaagttt ttacgcaca attcattcg gtcgtggaa ccaatgaag atggtgctg ctctcagac acgatgagt gacacccac gaagcaatc aagtttttt gtctcggcc tatcatttt aaaatcgat aacgccaac gaggggatt atagaaatg ttaaaaatg atgcgaact aaccacgaa gaacgcaaa agtgcggtc actgaggcg ctccgaacc atttatctg gtgactgat acgaagagt atggaggag cacacgacg actatttta aaccgcctg tttgcacta ttgaggaat ttaacctac gaccgcttg gaggacttt caagacgtt gtctcggaa tggaccgcc aagggcatt ctggacaac gctgttatt aacaggtgt tggcagtat tatacccag cacgagcac gtttccgcc gaagatgcc cgagcagcg gccgtgttg ctccgaatg gctgcgatg ggcagaaag acgataata acaaaaaac ctgaatgtt gttgcaaca atggcgttc agccaacaa tacaagagc gatatgttg tttttggcg aattcattt cggctcctg tcggtggca ggtcttgac aaaatcgat gttcaaagt gaggaaaca ccgttcaga atcaagaaa acggaggat ttgtttcac cagttatcg gataactta atcgagaat tttttcaat gatttgccg tactattcc gaagctatg caagcctgt accgacttt accttccga atgtgctcg aaaccgatg gaaattatc gagaatatt attaaagaa attgatgag cggttcaga cagcggccg caaacaatc gttttaagt attaggttt cttgaaatg ttggggtgt atagcgttg aaatttttg gagtatttg gacaacggc gtgcaccgg gaaatgaaa aggaggact tacctgaga caagagcgc gaaaaggcc aaagccaat aaaaagaat aagaggaaa agtaaaaga cagagtgtg cttgacacc tctaatgca agcgatgtc agtctgagt ggaagtatc gtgctgggg gctgaagct gaagacgtc gacactgaa tatataata gaggcttta gaaaacgac gtcgtctcc aagaaaggt gttttagga caatatgcc ccttttgta ctaaagttc tgtcaaaga ccggacatt tacagcgat gttttgctg caaacagct gcagtaaca acgctttcc aggtacatg ttgatttca agcaagttc tgtgccgaa aacatccgg ctgttgttc acgattttg gcaaaaacg agtcatcct gatatcaga tcgacgatt tcgatgcat tgtgtcgat ttgttgact agatttccg aatataatc gacccatgg agtccgcga ctttccgaa atgttaaaa gattcagta gccgaagtg cggaaaaat gcatttttc tcactgtcg aatttaatt ttgagagat atgatcaga gcatcgagt cgcatatcg gagatggcg gtttgtttg attgatccc gatccggaa gtcagccaa ctttgtaga tcctttttc acaaaaatt gctcaaaaa gacaacaat aacatcgtt agtctcatt cctgatatc ttcagttat ttggtgaaa caagaggag atcactgag gctgatcta agatacgtc ataaagttc ttgtttgat ttaatcgat gtgccaaaa cggccggaa aacttggtt gaccgtctg tgccacaaa tacacacca gacgaaaac gtcagactc aaccggaat ataacttat tgcctgtct ttaataaat tacaacgac aaggccttg caaaaatta cgcgatatg ttcccgctt taccggcac aatgtcaca gatgaggaa gtttactcg acctttaaa cagatattg aatgagtgt ggcaaggca aaagtcggt cgagttgat ttaaaacca attgttacg gaaatcgag aattgtatt gcgtccgtt tttgaaatt cacgaaggc gggccccct gttaaacca ccaacggtt gcaaaatcg ggaagaacg ggacgaaag aaaaagggg ccagcaaaa agggcgaga aaagtgcag tcagatagc gaggaagag gactcaggg gaggatgcg ctacctaaa agtacacct agcagaaat aagacgtca agaaacaga agaaattct taa
- >TC007535 RA
gacgaggtc gacatctgg caacgtgta tttatgttt ttcaaaccg tcagcgtca gtggttgtc attgtgttt ttagctata agtttgtgt atattttat catttttta aataattac tgacataaa aaatggacg gggttgtct tttcaatcc ccacaaatc gagccgatc ttctgaacg aaagtgaaa gaaaataca ctgtgaaga atgtcgtgg tttcttctg aaatcctca aaaaacttg aagaggctc gaacagggc ttctcgaag agggcccgt actttattt tggacaatt tcgacacct actattcca ttttgcatc aagaaaata agctatcca tcgatgtaa ttcagaaag ggttcaaca gtttactga aggcggtca agcagctca acgacacga tcctcttcc tggtcgaag acccggcca gtctgagtg aggaatcaa agtcgaaaa tggccaacg ttttaaaaa tgctgacgt acatctttg tccagttcg tcctgtact tggaggaaa aaaacaaac gttctggcg acttactaa gcaagggta aaaaggaga aaaagcacg ataacttcg gcgtaaaca aatccgagg ttgtgaaag cattgaata acataattc aaagaccga taaacgtct tctggcaac cgccgattg tggaacacg agtacatcg agttgttga gcgaattat gtttcggtc ttttacaaa acccgtcca taaaacagg aaaaagtgg tgattatgg acatattcg gcgtgtttg gtaaattgg taaagtcgt acgacttcg gcgagactt tcgttgcgc gaatgatac gaaacgtgt gcatgtacg aacatgtag tacactgtg ttcccgagg gaatcaaaa tgatggtgg agcagttta gttgccaaa acttggttc atcacctca tcattgagg cgacggaat ggcaagcga ctgagacag tccaagacg ttcaaggga cacggtgct gttccattt ttctgacag agttggcca aaacggtcc ccaacttag tcttccctg aagtaaccg atctcagtc tctatctag cacaccaat ctgttccgt tacgtacag ccgttctta acgtcatta ctgaaatta ttttacacg ttttaacaa gacacgact taaatcagg aagagaaag actcgcgcg ctacgtgct tcgatattt taacggaac acattttgg aatattcgc ccataattc gggcgaaag ttatacaaa acttgaaca ggctccaga aggaaaatg ccatacctt tggtcattc agaacgatg ttttaagta aagtggtgc gacatttgt cggataaat cggcaacgg tgaggaaat gtgcagtca gctgtgtta ccacctttc tggagtgta atccattcg gggcaaaac tccaactat ccgaagctg aggaacaac tagcgcaaa aaatcgaat tgttgcgaa aaatggaag cagcttacc aggaacagt tggtggcaa aagtgcaag aactggaag ctgggtggt taacgattg agaacgatt tgttagttg tggttacgg aagaattaa agacagacg aggatgaag atgaggacg aaagagagt cagtcgaga gtgaacaat ttgcggagc aaattcaat ctctcctgc atgaaaaaa aatacgccg aagcgttcc gaatgtgtc gccgcttca caaacaaca ttccggagt ctcgaaatg ttgcgaacg aagatcaag ccaaattct acatgaata ttctgcgct cgttttata tcaacactg ttaaatttt taacagaaa tgcgaacca cggccggta tgagccagg aagatgttg agaaaatta aggcactgg aaggggtca ttaaatatt tcgaagaca tggtcaagt ttttacgca caattcatt cggtcgtgg aaccaatga agatggtgc tgctctcag acacgatga gtgacaccc acgaagcaa tcaagtttt ttgtctcgg cctatcatt ttaaaatcg ataacgcca acgagggga ttatagaaa tgttaaaaa tgatgcgaa ctaaccacg aagaacgca aaagtgcgg tcactgagg cgctccgaa ccatttatc tggtgactg atacgaaga gtatggagg agcacacga cgactattt taaaccgcc tgtttgcac tattgagga atttaacct acgaccgct tggaggact ttcaagacg ttgtctcgg aatggaccg ccaagggca ttctggaca acgctgtta ttaacaggt gttggcagt attataccc agcacgagc acgtttccg ccgaagatg cccgagcag cggccgtgt tgctccgaa tggctgcga tgggcagaa agacgataa taacaaaaa acctgaatg ttgttgcaa caatggcgt tcagccaac aatacaaga gcgatatgt tgtttttgg cgaattcat ttcggctcc tgtcggtgg caggtcttg acaaaatcg atgttcaaa gtgaggaaa caccgttca gaatcaaga aaacggagg atttgtttc accagttat cggataact taatcgaga attttttca atgatttgc cgtactatt ccgaagcta tgcaagcct gtaccgact ttaccttcc gaatgtgct cgaaaccga tggaaatta tcgagaata ttattaaag aaattgatg agcggttca gacagcggc cgcaaacaa tcgttttaa gtattaggt ttcttgaaa tgttggggt gtatagcgt tgaaatttt tggagtatt tggacaacg gcgtgcacc gggaaatga aaaggagga cttacctga gacaagagc gcgaaaagg ccaaagcca ataaaaaga ataagagga aaagtaaaa gacagagtg tgcttgaca cctctaatg caagcgatg tcagtctga gtggaagta tcgtgctgg gggctgaag ctgaagacg tcgacactg aatatataa tagaggctt tagaaaacg acgtcgtct ccaagaaag gtgttttag gacaatatg ccccttttg tactaaagt tctgtcaaa gaccggaca tttacagcg atgttttgc tgcaaacag ctgcagtaa caacgcttt ccaggtaca tgttgattt caagcaagt tctgtgccg aaaacatcc ggctgttgt tcacgattt tggcaaaaa cgagtcatc ctgatatca gatcgacga tttcgatgc attgtgtcg atttgttga ctagatttc cgaatataa tcgacccat ggagtccgc gactttccg aaatgttaa aagattcag tagccgaag tgcggaaaa atgcatttt tctcactgt cgaatttaa ttttgagag atatgatca gagcatcga gtcgcatat cggagatgg cggtttgtt tgattgatc ccgatccgg aagtcagcc aactttgta gatcctttt tcacaaaaa ttgctcaaa aagacaaca ataacatcg ttagtctca ttcctgata tcttcagtt atttggtga aacaagagg agatcactg aggctgatc taagatacg tcataaagt tcttgtttg atttaatcg atgtgccaa aacggccgg aaaacttgg ttgaccgtc tgtgccaca aatacacac cagacgaaa acgtcagac tcaaccgga atataactt attgcctgt ctttaataa attacaacg acaaggcct tgcaaaaat tacgcgata tgttcccgc tttaccggc acaatgtca cagatgagg aagtttact cgaccttta aacagatat tgaatgagt gtggcaagg caaaagtcg gtcgagttg atttaaaac caattgtta cggaaatcg agaattgta ttgcgtccg tttttgaaa ttcacgaag gcgggcccc ctgttaaac caccaacgg ttgcaaaat cgggaagaa cgggacgaa agaaaaagg ggccagcaa aaagggcga gaaaagtgc agtcagata gcgaggaag aggactcag gggaggatg cgctaccta aaagtacac ctagcagaa ataagacgt caagaaaca gaagaaatt cttaaaaca gacagttgt attattgtt actttcaat aaagttttt
