Status
Please wait ...
Gene information
General information
TC007531 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3306888..3308208
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007531
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01214

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 72
    Pupal morphology
    remarks: later than schedule, hours 72
    Adult morphology
    remarks: later than schedule, hours 72
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007531 RA
      MAFGRRMDG CGNFMKYSL FIVNIIILL 
      GGLIVAGLG LWTVIDKSF ANELLGTNL 
      YSGAAYVLV ITGLLVSFI SCFGCFGAI 
      REVRCMLVT YSIALFLIF VTMLIGGVL 
      GYVFREKVD TTVRNGMLG SLKSYGNYR 
      PVTEAWDET QSRLMCCGV NDWKDWRDN 
      IPDSCCKPV LGRRQRCNL LVENQNSFT 
      LYTRGCLEI TKEYVKDHA VVIGSAGIV 
      VACLMIFGM IFSCVLFKL LE
    CDS sequence
    • >TC007531 RA
      atggctttc ggccgccga atggacggc tgcggcaac 
      ttcatgaag tatagcctc ttcatcgtc aacataatt 
      atactattg ggtggcttg attgtggcc ggtctaggt 
      ctatggacc gtcatcgac aagtccttc gccaacgaa 
      ctcctaggc acgaatctc tactccggt gccgcttac 
      gttctcgtg atcaccggc cttttggtc tccttcatc 
      tcctgcttc ggctgtttt ggcgcgatt agagaagtc 
      cgctgcatg ctagtcacg tactccatc gccttgttc 
      cttattttc gtcacgatg ctgatcggg ggcgtcctc 
      ggttacgtt ttccgggag aaagtcgat acgacggtc 
      aggaatggc atgctgggc tccctgaaa tcctacggg 
      aactacaga cccgtgacg gaggcctgg gatgagacc 
      cagagccga ctgatgtgc tgcggtgtc aacgactgg 
      aaggactgg agggacaac atcccggat tcgtgctgc 
      aaaccggtg ctggggcga aggcagaga tgcaatttg 
      ttggtcgag aatcaaaat tcgttcacg ttgtacacg 
      agaggttgt ttggaaatc accaaggag tatgtgaag 
      gaccatgcg gttgtcatt ggatcggcg gggatcgta 
      gtggcttgt cttatgatt ttcggaatg atcttttcg 
      tgcgtcctt ttcaaattg ctcgaatga
    mRNA sequence
    • >TC007531 RA
      tttcactgc tttttactc tctcgcatg taattagtt 
      tttcgtaat tagaattag agtgtttcc tagtcgaaa 
      tttctgcta aaaggagta acacacaaa aagcgtgat 
      tcttgattc atttggtcg ttattgacc acaaggagt 
      ggaggggcg acacgcaaa aacaacaaa cacaacata 
      caggtgtgt ggcgtcgtc caacgtgac tcttcttcg 
      actccaacc gcccaaaac ccgaaaaca tccccgaaa 
      tggctttcg gccgccgaa tggacggct gcggcaact 
      tcatgaagt atagcctct tcatcgtca acataatta 
      tactattgg gtggcttga ttgtggccg gtctaggtc 
      tatggaccg tcatcgaca agtccttcg ccaacgaac 
      tcctaggca cgaatctct actccggtg ccgcttacg 
      ttctcgtga tcaccggcc ttttggtct ccttcatct 
      cctgcttcg gctgttttg gcgcgatta gagaagtcc 
      gctgcatgc tagtcacgt actccatcg ccttgttcc 
      ttattttcg tcacgatgc tgatcgggg gcgtcctcg 
      gttacgttt tccgggaga aagtcgata cgacggtca 
      ggaatggca tgctgggct ccctgaaat cctacggga 
      actacagac ccgtgacgg aggcctggg atgagaccc 
      agagccgac tgatgtgct gcggtgtca acgactgga 
      aggactgga gggacaaca tcccggatt cgtgctgca 
      aaccggtgc tggggcgaa ggcagagat gcaatttgt 
      tggtcgaga atcaaaatt cgttcacgt tgtacacga 
      gaggttgtt tggaaatca ccaaggagt atgtgaagg 
      accatgcgg ttgtcattg gatcggcgg ggatcgtag 
      tggcttgtc ttatgattt tcggaatga tcttttcgt 
      gcgtccttt tcaaattgc tcgaatgag cacaagtct 
      ctcggtgtt ttactttct taaatgtat ttatctaac 
      ttcatttat aagatactt attaaaata aatgttttt 
      ttataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY