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Gene information
General information
TC007530 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3307930..3311308
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007530
    iBeetle screen iB_01213

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007530 RA
      MTSLRQKQI NALKQMLNL NQPQSKLSA SEPKWKVLV 
      YDNVGQDII SVLISVKEL RELGVTLFV QLHSDRDPI 
      PEVPAVYFC APTEENLGR IAQDFQKGV YDIYHLNFI 
      SAISRQKLE DLASAALQA NCVANIHKV YDQYVNFIS 
      LDDDMFILK NQNSDALSY YAINRGDTK DTEMDEIMD 
      KIVDCLFSV FVTLGTVPI IRSPKGNAA EMVARKLDK 
      KLRENLADA RNNLLHSDA QAGNFNFQR PLLIVLDRN 
      VDMATPLHH TWTYQALAH DLLNLNLNR VVIEESVPS 
      GGVRSKNRV CELDTKDKF WSTHKGSPF PTVAEAIQE 
      ELEQYKSSE EEVKKLKSS MGIDGESDI ALSLVSDNT 
      AKITSAVNS LPQLLEKKR LIDMHTTLA TALLNGIKS 
      RKLDNFFEL EEKIMSKTQ ALEKTIYDL IVDPETGVP 
      EDKIRLFII FYICSPHMS ESELKRYET ALSEAGCDL 
      SPIHYIKRW KSYAKMASG SNQYEGAGT KTVNMFSKL 
      VSHGSNFVM EGVKNLVIK RHNLPVTRI VDHLMEFKN 
      SPEMDEYHY LDPKQLKVT EIPRNRSPF QDAIVFIVG 
      GGNYIEYQN LVDYARQKT AAGSTKRIT YGSSTLANA 
      QQFLKQLSL LGQEIV
    CDS sequence
    • >TC007530 RA
      atgacttca ctgcgtcaa aaacaaatc aatgccctc 
      aaacaaatg ttaaacttg aatcaaccc caaagtaag 
      ttaagcgcc tcggaacca aaatggaaa gtattagta 
      tacgacaac gtaggacag gacattatt tcggtatta 
      atatcagtc aaggaattg agggaacta ggggtcacg 
      ttatttgtc cagttgcac tcagaccgg gaccctata 
      cctgaagtc cccgcggtt tatttctgt gcccccact 
      gaggaaaat ctcggccgg atagcacaa gattttcaa 
      aagggggtc tacgatatt tatcatttg aattttatt 
      tctgccatt tcacggcaa aaactggaa gatcttgcc 
      tcggcggcc ttgcaagcg aactgcgtg gccaacata 
      cacaaagtt tacgatcag tatgttaat tttatttcg 
      ctagatgat gacatgttt atactaaaa aaccaaaac 
      agtgacgcc ttatcatat tacgcgata aatcggggc 
      gacaccaaa gacacggaa atggacgaa atcatggac 
      aagatcgtc gactgttta ttctccgtg tttgtcact 
      ttgggcaca gtccccata atccggagc cccaaaggc 
      aacgccgct gaaatggtc gcgaggaaa ttagacaaa 
      aaactgcgc gaaaatctg gccgatgct cgaaataac 
      ttgctccat tcggacgcc caagcgggg aattttaac 
      ttccaaagg cccctttta atcgtcttg gaccgaaat 
      gtggacatg gccacaccc cttcaccac acttggacc 
      tatcaagcc ctagcgcac gacctactc aacctaaac 
      ctaaaccgc gtcgttatc gaagaaagt gtgccaagc 
      ggaggcgtc cgctcgaaa aaccgggtg tgtgagttg 
      gacacgaaa gacaagttc tggtcaaca cataaaggc 
      tcgccgttt cccacagtt gcggaagcg atccaggaa 
      gagctagag cagtacaag tcgtcggaa gaggaagtg 
      aagaagttg aaaagttcg atggggatt gacggagag 
      agcgacatc gctctgtcg ctagtttca gataacacg 
      gcaaaaatt acctcagct gttaactcg cttccgcaa 
      ctgttggag aagaaacgg ctaattgac atgcacacg 
      actctcgcg acggcactt ctcaacgga ataaaatcg 
      cggaaattg gacaacttc ttcgaactg gaggagaaa 
      atcatgagt aaaacccaa gcgttggag aagacgatc 
      tacgacttg atcgtggac ccggaaacg ggcgttcca 
      gaggacaag atccgctta tttatcatt ttttacatt 
      tgcagccca catatgagc gaaagtgag ttaaaacgc 
      tacgaaacg gccttatca gaggccggg tgcgacctc 
      tcacccatc cattacata aaaaggtgg aagagttac 
      gcaaaaatg gcgtccggt tcgaaccaa tacgaaggc 
      gccggtaca aaaacagta aacatgttc tcaaaactt 
      gtctcacac ggctccaat ttcgtgatg gaaggggtg 
      aaaaatttg gttattaag cgacataat ttaccggtg 
      acacgtatt gtggatcat ttgatggag tttaagaac 
      agtccggag atggacgag taccactac cttgaccct 
      aaacagtta aaagtgact gaaattccg cgtaaccgc 
      tcgccgttc caggacgct atcgttttc attgtcggt 
      gggggcaat tacattgag tatcagaat ttggtggac 
      tatgctagg caaaaaacg gcggcaggg agcaccaaa 
      cggatcacc tacgggtcg tccacgctc gccaacgcg 
      caacagttt ttaaagcaa ctttcgttg ttagggcag 
      gaaattgtg taa
    mRNA sequence
    • >TC007530 RA
      cactttgta aaaaatgaa gattttggc ccaaaaatg 
      acccaattt tgccacgat ttcaatttt ttatgttgg 
      caagtgggg tagttgtca aatgaacac gtcagctgt 
      ttatcgcgt aattttcaa aaaaaatct ttctccgaa 
      aataagaca gcactaaaa tcacccctt tgtgtcaaa 
      ttcgcccca caatgactt cactgcgtc aaaaacaaa 
      tcaatgccc tcaaacaaa tgttaaact tgaatcaac 
      cccaaagta agttaagcg cctcggaac caaaatgga 
      aagtattag tatacgaca acgtaggac aggacatta 
      tttcggtat taatatcag tcaaggaat tgagggaac 
      taggggtca cgttatttg tccagttgc actcagacc 
      gggacccta tacctgaag tccccgcgg tttatttct 
      gtgccccca ctgaggaaa atctcggcc ggatagcac 
      aagattttc aaaaggggg tctacgata tttatcatt 
      tgaatttta tttctgcca tttcacggc aaaaactgg 
      aagatcttg cctcggcgg ccttgcaag cgaactgcg 
      tggccaaca tacacaaag tttacgatc agtatgtta 
      attttattt cgctagatg atgacatgt ttatactaa 
      aaaaccaaa acagtgacg ccttatcat attacgcga 
      taaatcggg gcgacacca aagacacgg aaatggacg 
      aaatcatgg acaagatcg tcgactgtt tattctccg 
      tgtttgtca ctttgggca cagtcccca taatccgga 
      gccccaaag gcaacgccg ctgaaatgg tcgcgagga 
      aattagaca aaaaactgc gcgaaaatc tggccgatg 
      ctcgaaata acttgctcc attcggacg cccaagcgg 
      ggaatttta acttccaaa ggccccttt taatcgtct 
      tggaccgaa atgtggaca tggccacac cccttcacc 
      acacttgga cctatcaag ccctagcgc acgacctac 
      tcaacctaa acctaaacc gcgtcgtta tcgaagaaa 
      gtgtgccaa gcggaggcg tccgctcga aaaaccggg 
      tgtgtgagt tggacacga aagacaagt tctggtcaa 
      cacataaag gctcgccgt ttcccacag ttgcggaag 
      cgatccagg aagagctag agcagtaca agtcgtcgg 
      aagaggaag tgaagaagt tgaaaagtt cgatgggga 
      ttgacggag agagcgaca tcgctctgt cgctagttt 
      cagataaca cggcaaaaa ttacctcag ctgttaact 
      cgcttccgc aactgttgg agaagaaac ggctaattg 
      acatgcaca cgactctcg cgacggcac ttctcaacg 
      gaataaaat cgcggaaat tggacaact tcttcgaac 
      tggaggaga aaatcatga gtaaaaccc aagcgttgg 
      agaagacga tctacgact tgatcgtgg acccggaaa 
      cgggcgttc cagaggaca agatccgct tatttatca 
      ttttttaca tttgcagcc cacatatga gcgaaagtg 
      agttaaaac gctacgaaa cggccttat cagaggccg 
      ggtgcgacc tctcaccca tccattaca taaaaaggt 
      ggaagagtt acgcaaaaa tggcgtccg gttcgaacc 
      aatacgaag gcgccggta caaaaacag taaacatgt 
      tctcaaaac ttgtctcac acggctcca atttcgtga 
      tggaagggg tgaaaaatt tggttatta agcgacata 
      atttaccgg tgacacgta ttgtggatc atttgatgg 
      agtttaaga acagtccgg agatggacg agtaccact 
      accttgacc ctaaacagt taaaagtga ctgaaattc 
      cgcgtaacc gctcgccgt tccaggacg ctatcgttt 
      tcattgtcg gtgggggca attacattg agtatcaga 
      atttggtgg actatgcta ggcaaaaaa cggcggcag 
      ggagcacca aacggatca cctacgggt cgtccacgc 
      tcgccaacg cgcaacagt ttttaaagc aactttcgt 
      tgttagggc aggaaattg tgtaatttt atatttaat 
      aaagtattg ttattagtt ggtgtttta ttgttatta 
      cacggaaag gtacttttt tccgtacag ttgggcctt 
      tttctcgaa cgcggccga cttgatcac ggtattttc 
      ctcgtaaaa cgggttgag ggagtgttt gatgtacgc 
      ctcgtatat ttcggagaa gaagttctt aatgccgtc 
      atcattcct attatcgtg gaccataac gaaccttat 
      caggctcgc cgttacaaa agcggagac aaaccattg 
      gttgaattt gtccactga ttttaaatt catattatg 
      ggttttcca tttgtgttc gtcgattaa gtccaaagc 
      ggaatgggc gataaattg gtttaaatg tctatggtc 
      ctccttcta caaccctcc aacctcagc agtctcaaa 
      ggggagtcc ccgaaccta ccttagggt ctttactat 
      tcgtaaact ccatttcaa acaaaggca aatccttat 
      gacccacga tcacaaaat aataacttc cgatcattt 
      tattcgcct tcattaacg ataatttcg cccaaattt 
      agccaaaaa acataactt gataatttg gaggttacg 
      tcgattgcc agcactgac gcgccacat tcaaagaat 
      tgggttaag cgcgcacct ggtccgtga ccgtgaact 
      taattactc aaatgtgaa ctttcactg ctttttact 
      ctctcgcat gtaattagt ttttcgtaa ttagaatta 
      gagtgtttc ctagtcgaa atttctgct aaaaggagt 
      aacacacaa aaagcgtga ttcttgatt catttggtc 
      gttattgac cacaaggag tggaggggc gacacgcaa 
      aaacaacaa acacaacat acaggtgtg tggcgtcgt 
      ccaacgtga ctcttcttc gactccaac cgcccaaaa 
      cccgaaaac atgtgagtg cccaattca ataaatttg 
      atttttatt c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY