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Gene information
General information
TC007529 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3315752..3319998
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007529
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01212

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    remarks: later than schedule, hours 96 pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007529 RA
      MASILRSSK FVRYIAGFA RNVVLNTPR EFDGNLVNT 
      TQCLCGAVP RSFASQIPA PHDQNLETS LRRLDQDVR 
      KVGRISRRD IEDVLDEIR SSRSATSSQ SLLVIRCCG 
      NLVPEELPE VRTKLVQEI WATLNKLNV PMDISHYNA 
      LLRVYLENE HQFSPAEFL ADLESKGIE PNRVTYQRL 
      IARYCQVGD IEGATKILE FMKEKQLPV NENVFNALI 
      VGHSQTGDM DSAQGILGV MTQAGLEPS ADTYTTLLC 
      GFGKKGDID AINKLFEEC ESKEIYLLD KDYLDIVYA 
      LATNGHSEH IPQILSKVR RAIGYNQDA INLILRLIN 
      KGLEDAAFP VLETVVRST NPDGTHNPV GNFFVKQMV 
      KANRPVEKI IEMCNKLES SGMYTRGLS LAAETSLEF 
      GNDQLAYSL LTELSKKDI PVRQHYFWP LIVAKSKDS 
      SGQGILDVL LSMHKFNIT PSNETLREY VIPNLKAPS 
      SEILSILRS ANVPLGTSG ACLVHHLLN KSDIKEAAI 
      ISTQVSAFY NPDLLKRPL TNSFYKNKD LESYIRIVR 
      NIYDNLDRL KRSEEDEPT QVADKNEVV GNLVLDLSW 
      NREFLNVVE DVLKGLISE GLSISTTAA EKLQEKLGE 
      KMTEEISSL LGKLTSGHL TPVPVPKKL PSYTPSHQM 
      NVEQLERLI HNLEVKNQD TKGLKRQLL TLYYREKDL 
      EKTESLLAE LEKTDFVYS AGIYAQLSD LYAHHDKLD 
      KAMEYYNKL KELEGNEFK MDETKIVSI AHLLVRNGK 
      FDQAIEIIE KTPRENLRE ERTYNYAAQ VWRLLNSLA 
      EEGRVDEVN KLFDTLVKN DFIEVNNIL LGPLVKVHL 
      VNKDIDKAM EKFEWCVNQ FRATPWKNE LACQLIQKE 
      DAEKLQKLT DLSTSIHGE INSLYDLVF AFVECGRVR 
      QARKILETP GLQTRPQRI NIRCERYRQ EGQVKPLES 
      LKDATKDLD HINRSDIYY QLLLSYIKQ EEVEKALGL 
      WTQMQEEDL LPSDEFLQS LGKFLQDKG VSVPFQIPQ 
      SVTPPQVVE KSHIQQFRR KLKEGDVQS ALQFYNPND 
      HSFTVTDTS LLIEKLIQN NRIGEATKV TLNLLETGA 
      TPLARVFKF LLNKLANAG DLDSLAIID KKVSQEVKK 
      TISFDNRVC HANLIAGKA PQYLDQLEN EIDNAKDED 
      LKAIEEKFP RGGAYGILE RHPQLLEKY ENLAVKYAQ 
      RGIVAPMNV LWTNYFIVG NEPKAEQIW NTYLKGAPR 
      IMFQRIVHA ARETQDENL IKKLIKHLK NSNVTEGAV 
      GNAYSCLLD VLVAKNNTE EVVLAFELA LKDAGLNHL 
      NRTAILRVK EVYEKLGKP FDYAIPPKN PRIATSTSS 
      GEEDAKK
    CDS sequence
    • >TC007529 RA
      atggcttcg attttacgc tcttcgaaa ttcgtccgc 
      tatatcgcc ggttttgca aggaacgta gtgttgaac 
      actcctcgg gagttcgat gggaacctc gtcaacacc 
      acacaatgt ctttgcggc gcagtcccg cgctccttc 
      gcctcgcaa ataccggca ccacacgac cagaacctc 
      gaaaccagc ctcagaaga ctcgaccaa gacgtgagg 
      aaagtcggg agaatctcg cggcgtgac attgaagac 
      gtcttagac gagatcaga agcagcaga tcggccacc 
      agctcccag tcactcctc gtgatacga tgttgcggg 
      aacttggtc ccggaagaa ctccccgaa gtgcgcacc 
      aaactggtc caggagatc tgggcgacg ttaaacaaa 
      ctgaacgtc cccatggac atttcgcat tacaacgca 
      cttttgcgg gtctatttg gaaaatgag caccaattt 
      tcgccggcc gagtttttg gccgacttg gaaagcaaa 
      ggaattgag ccaaaccga gtcacttat caacgctta 
      atcgcacgc tattgccaa gtgggggat atcgaaggt 
      gcgaccaaa atcctcgaa tttatgaaa gaaaagcaa 
      cttccggtc aacgaaaac gtcttcaat gcgcttatc 
      gtgggacac tcgcagacg ggtgacatg gactcagct 
      cagggtatc ttgggggtg atgacacag gccggtctc 
      gagcccagc gcagacacc tacactacc cttctatgc 
      ggtttcggc aaaaagggc gacattgac gcgataaat 
      aaattgttc gaggagtgc gaaagcaag gaaatttac 
      ctcctggat aaagattat ctcgatatt gtgtacgcc 
      ttagccacg aatggccac agcgaacac atcccgcaa 
      attttgagc aaagtcaga cgcgcaatc ggttacaac 
      caagacgct attaacttg attttgcgt ctgattaac 
      aaaggacta gaggacgca gccttccct gtccttgag 
      acagtagta agatcaaca aaccccgac ggaacacat 
      aaccccgtt ggtaatttc tttgtaaaa caaatggtc 
      aaagccaac cgcccggtt gaaaaaatc atcgaaatg 
      tgcaataaa cttgaaagt agtgggatg tacacacgg 
      gggttgtcc ctcgctgct gagacaagt ctcgagttt 
      ggaaacgac caactcgcc tattccctg ttaaccgaa 
      ctgagtaag aaagacatt ccggtcaga caacactat 
      ttctggcct ttaatagtc gcgaaaagt aaagattca 
      tctggacag gggatttta gacgtcctc ttatcaatg 
      cacaagttt aatattacg ccaagcaac gagacactg 
      agagagtac gttattccg aatttgaaa gccccaagt 
      agcgaaatt ttgtcgatt ttgcgctcg gctaacgtg 
      ccattaggg acgtcaggg gcttgtctc gtccatcat 
      ttattaaac aaaagcgac ataaaggaa gccgcgatt 
      atttcaacc caagtatcg gctttctac aatccggat 
      ttattgaaa cgtcctttg actaactcg ttttacaaa 
      aacaaagat ttggagagt tatatcaga atcgtgcgc 
      aacatttac gacaacttg gaccgactt aaacgcagc 
      gaagaggac gaaccgact caggtcgct gataaaaac 
      gaagttgtg gggaaccta gtgcttgat ttatcctgg 
      aatcgggag tttttgaac gttgtcgaa gatgtgctt 
      aagggtttg atttctgaa ggtttgagt atttccaca 
      actgcggct gagaagctt caagagaaa ctaggtgag 
      aagatgacc gaagaaata tccagtctt ctcgggaaa 
      ttgacttct ggtcattta actccggtt ccggttccg 
      aaaaaacta ccatcgtac acaccgtcg catcagatg 
      aatgtcgag caattagag cgtctcatt cataacttg 
      gaagttaaa aaccaggat actaaaggc ttgaaacgc 
      cagttgtta actttgtat tatcgggag aaagatttg 
      gaaaaaacc gagtcactt ttggccgag ttggaaaaa 
      accgatttc gtgtactca gccgggatt tatgcccaa 
      cttagcgac ttgtacgcc caccacgac aagttggac 
      aaagctatg gaatattac aacaaactg aaagaactg 
      gaaggcaac gaattcaaa atggacgag acgaaaatc 
      gtgtcaatt gcccacctt ttggtcaga aacggcaaa 
      ttcgaccaa gcgatcgaa atcatcgaa aaaacccca 
      cgtgagaat ctaagggag gaacgcact tacaattac 
      gcggcccaa gtgtggcga ctattgaat tcgttggcc 
      gaagaaggc cgagtcgac gaagtgaac aagctattc 
      gatactttg gtcaagaac gacttcatc gaagtgaat 
      aacattctc ttggggccc ctagttaag gtccatttg 
      gtcaataaa gacattgac aaggctatg gaaaaattc 
      gaatggtgt gtgaaccaa ttccgggca acaccgtgg 
      aaaaacgaa ttggcgtgc caactaatc cagaaggaa 
      gatgcggaa aaattacaa aaactgacc gacttatcg 
      acctccatt cacggcgaa atcaacagt ctctacgac 
      ctggttttc gctttcgtc gagtgcgga cgagtgaga 
      caagccagg aaaattctg gaaactccc ggcttgcaa 
      acgcgcccc cagcgaatc aacatccgg tgtgagaga 
      taccgacag gagggtcaa gtcaagccg cttgaaagc 
      ttaaaagac gccactaaa gacttggac catatcaac 
      agaagcgac atttattac caactgttg ttgagttac 
      atcaagcaa gaggaggtg gagaaggcg ctagggctt 
      tggacgcaa atgcaggaa gaggatttg ttaccgagc 
      gacgagttt ttgcaaagc ttagggaaa tttttacaa 
      gataagggt gtttccgtc ccgttccaa atcccgcaa 
      tcggtgacc ccaccacaa gttgtagaa aagtcacat 
      attcaacag tttcgccgc aaattgaaa gaaggcgac 
      gtacaaagc gccttgcaa ttctataat cccaatgat 
      cacagtttc acagtcact gatacgtcg ttacttatc 
      gaaaagttg atccaaaat aaccgaatt ggggaagcg 
      accaaagta acgttgaat ttactggag acgggagcg 
      accccactt gcccgagtt ttcaaattt ttgttaaac 
      aagttggca aatgccggc gatttggat tctcttgca 
      atcatagat aagaaagtt tcacaagaa gttaagaaa 
      acaatctcg tttgataac cgcgtttgc catgcgaat 
      ttaatcgcc gggaaagca ccacaatac ttggatcaa 
      ttagaaaac gaaatcgac aatgccaaa gatgaggac 
      ttgaaggcc attgaggag aagttcccc cgcgggggg 
      gcttacggt atcttggaa cgccatccc caattgttg 
      gaaaaatat gaaaatctg gcggttaaa tacgcgcaa 
      agggggata gtggcgcca atgaacgtc ctctggacc 
      aactacttc attgtgggg aatgagccg aaggcggag 
      caaatctgg aatacttac ctcaagggc gcccccagg 
      attatgttc caaaggata gtgcacgcc gcccgcgag 
      acacaagac gaaaatctc atcaaaaaa ctaatcaaa 
      cacttgaaa aacagtaac gtgactgaa ggagccgtc 
      ggtaatgcg tacagttgt ttgcttgat gtcttggtg 
      gcgaaaaac aacactgaa gaagtggtt ctggcgttc 
      gaattagcc ctcaaagac gccggtttg aaccacttg 
      aaccgaacc gcgatactg cgagtcaaa gaagtgtac 
      gaaaaatta ggcaaacct ttcgattac gcaattccg 
      ccaaagaat cctcgaatc gccacgagt acgtcgtcc 
      ggtgaagag gacgcaaaa aagtag
    mRNA sequence
    • >TC007529 RA
      ctaaaacca cgagaagca tggcttcga ttttacgct 
      cttcgaaat tcgtccgct atatcgccg gttttgcaa 
      ggaacgtag tgttgaaca ctcctcggg agttcgatg 
      ggaacctcg tcaacacca cacaatgtc tttgcggcg 
      cagtcccgc gctccttcg cctcgcaaa taccggcac 
      cacacgacc agaacctcg aaaccagcc tcagaagac 
      tcgaccaag acgtgagga aagtcggga gaatctcgc 
      ggcgtgaca ttgaagacg tcttagacg agatcagaa 
      gcagcagat cggccacca gctcccagt cactcctcg 
      tgatacgat gttgcggga acttggtcc cggaagaac 
      tccccgaag tgcgcacca aactggtcc aggagatct 
      gggcgacgt taaacaaac tgaacgtcc ccatggaca 
      tttcgcatt acaacgcac ttttgcggg tctatttgg 
      aaaatgagc accaatttt cgccggccg agtttttgg 
      ccgacttgg aaagcaaag gaattgagc caaaccgag 
      tcacttatc aacgcttaa tcgcacgct attgccaag 
      tgggggata tcgaaggtg cgaccaaaa tcctcgaat 
      ttatgaaag aaaagcaac ttccggtca acgaaaacg 
      tcttcaatg cgcttatcg tgggacact cgcagacgg 
      gtgacatgg actcagctc agggtatct tgggggtga 
      tgacacagg ccggtctcg agcccagcg cagacacct 
      acactaccc ttctatgcg gtttcggca aaaagggcg 
      acattgacg cgataaata aattgttcg aggagtgcg 
      aaagcaagg aaatttacc tcctggata aagattatc 
      tcgatattg tgtacgcct tagccacga atggccaca 
      gcgaacaca tcccgcaaa ttttgagca aagtcagac 
      gcgcaatcg gttacaacc aagacgcta ttaacttga 
      ttttgcgtc tgattaaca aaggactag aggacgcag 
      ccttccctg tccttgaga cagtagtaa gatcaacaa 
      accccgacg gaacacata accccgttg gtaatttct 
      ttgtaaaac aaatggtca aagccaacc gcccggttg 
      aaaaaatca tcgaaatgt gcaataaac ttgaaagta 
      gtgggatgt acacacggg ggttgtccc tcgctgctg 
      agacaagtc tcgagtttg gaaacgacc aactcgcct 
      attccctgt taaccgaac tgagtaaga aagacattc 
      cggtcagac aacactatt tctggcctt taatagtcg 
      cgaaaagta aagattcat ctggacagg ggattttag 
      acgtcctct tatcaatgc acaagttta atattacgc 
      caagcaacg agacactga gagagtacg ttattccga 
      atttgaaag ccccaagta gcgaaattt tgtcgattt 
      tgcgctcgg ctaacgtgc cattaggga cgtcagggg 
      cttgtctcg tccatcatt tattaaaca aaagcgaca 
      taaaggaag ccgcgatta tttcaaccc aagtatcgg 
      ctttctaca atccggatt tattgaaac gtcctttga 
      ctaactcgt tttacaaaa acaaagatt tggagagtt 
      atatcagaa tcgtgcgca acatttacg acaacttgg 
      accgactta aacgcagcg aagaggacg aaccgactc 
      aggtcgctg ataaaaacg aagttgtgg ggaacctag 
      tgcttgatt tatcctgga atcgggagt ttttgaacg 
      ttgtcgaag atgtgctta agggtttga tttctgaag 
      gtttgagta tttccacaa ctgcggctg agaagcttc 
      aagagaaac taggtgaga agatgaccg aagaaatat 
      ccagtcttc tcgggaaat tgacttctg gtcatttaa 
      ctccggttc cggttccga aaaaactac catcgtaca 
      caccgtcgc atcagatga atgtcgagc aattagagc 
      gtctcattc ataacttgg aagttaaaa accaggata 
      ctaaaggct tgaaacgcc agttgttaa ctttgtatt 
      atcgggaga aagatttgg aaaaaaccg agtcacttt 
      tggccgagt tggaaaaaa ccgatttcg tgtactcag 
      ccgggattt atgcccaac ttagcgact tgtacgccc 
      accacgaca agttggaca aagctatgg aatattaca 
      acaaactga aagaactgg aaggcaacg aattcaaaa 
      tggacgaga cgaaaatcg tgtcaattg cccaccttt 
      tggtcagaa acggcaaat tcgaccaag cgatcgaaa 
      tcatcgaaa aaaccccac gtgagaatc taagggagg 
      aacgcactt acaattacg cggcccaag tgtggcgac 
      tattgaatt cgttggccg aagaaggcc gagtcgacg 
      aagtgaaca agctattcg atactttgg tcaagaacg 
      acttcatcg aagtgaata acattctct tggggcccc 
      tagttaagg tccatttgg tcaataaag acattgaca 
      aggctatgg aaaaattcg aatggtgtg tgaaccaat 
      tccgggcaa caccgtgga aaaacgaat tggcgtgcc 
      aactaatcc agaaggaag atgcggaaa aattacaaa 
      aactgaccg acttatcga cctccattc acggcgaaa 
      tcaacagtc tctacgacc tggttttcg ctttcgtcg 
      agtgcggac gagtgagac aagccagga aaattctgg 
      aaactcccg gcttgcaaa cgcgccccc agcgaatca 
      acatccggt gtgagagat accgacagg agggtcaag 
      tcaagccgc ttgaaagct taaaagacg ccactaaag 
      acttggacc atatcaaca gaagcgaca tttattacc 
      aactgttgt tgagttaca tcaagcaag aggaggtgg 
      agaaggcgc tagggcttt ggacgcaaa tgcaggaag 
      aggatttgt taccgagcg acgagtttt tgcaaagct 
      tagggaaat ttttacaag ataagggtg tttccgtcc 
      cgttccaaa tcccgcaat cggtgaccc caccacaag 
      ttgtagaaa agtcacata ttcaacagt ttcgccgca 
      aattgaaag aaggcgacg tacaaagcg ccttgcaat 
      tctataatc ccaatgatc acagtttca cagtcactg 
      atacgtcgt tacttatcg aaaagttga tccaaaata 
      accgaattg gggaagcga ccaaagtaa cgttgaatt 
      tactggaga cgggagcga ccccacttg cccgagttt 
      tcaaatttt tgttaaaca agttggcaa atgccggcg 
      atttggatt ctcttgcaa tcatagata agaaagttt 
      cacaagaag ttaagaaaa caatctcgt ttgataacc 
      gcgtttgcc atgcgaatt taatcgccg ggaaagcac 
      cacaatact tggatcaat tagaaaacg aaatcgaca 
      atgccaaag atgaggact tgaaggcca ttgaggaga 
      agttccccc gcggggggg cttacggta tcttggaac 
      gccatcccc aattgttgg aaaaatatg aaaatctgg 
      cggttaaat acgcgcaaa gggggatag tggcgccaa 
      tgaacgtcc tctggacca actacttca ttgtgggga 
      atgagccga aggcggagc aaatctgga atacttacc 
      tcaagggcg cccccagga ttatgttcc aaaggatag 
      tgcacgccg cccgcgaga cacaagacg aaaatctca 
      tcaaaaaac taatcaaac acttgaaaa acagtaacg 
      tgactgaag gagccgtcg gtaatgcgt acagttgtt 
      tgcttgatg tcttggtgg cgaaaaaca acactgaag 
      aagtggttc tggcgttcg aattagccc tcaaagacg 
      ccggtttga accacttga accgaaccg cgatactgc 
      gagtcaaag aagtgtacg aaaaattag gcaaacctt 
      tcgattacg caattccgc caaagaatc ctcgaatcg 
      ccacgagta cgtcgtccg gtgaagagg acgcaaaaa 
      agtagtgta attccagtt ataatttat attaaattg 
      tttttttta ataaaagtg tagttgatc a
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