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Gene information
General information
TC007521 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3422222..3424488
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007521
    iBeetle screen iB_01211

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007521 RA
      MGLIDETVS FYDKEASCA EDQIKCLKK QLLQLQQNI 
      KNKTVDTSQ NPELQKLVT ENTKLKHRL TILKRAIAS 
      LSHSQTSRD STRMQNIQE ILDEIFGAA ISSACPDIT 
      DAPVVITLS NNAKFGDYQ CNSAMQISN IYKQLGKKV 
      SPRDIANKI VENVSPHEL VDKLEVAGP GFINVFVSK 
      AFGLRNLST IFTHGVRPP PYKKLRVVV DLSSPNIAK 
      EMHVGHLRS TIIGDTICR LLEFLGHDV LRINHIGDW 
      GTQFGMLIV HLQEKFPDY LSKAPPISD LQAFYKESK 
      KRFDEDEDF KKRAYAGVV KLQSGDPNY IKAWQLICD 
      VSRKEFQVI YDRLDVKLI ERGESFYQS RMNDIVKEL 
      EAGGYLEDD EGRKVMWGD KNDGNIPFT VVKSDGGYT 
      YDTSDLATI KQRIVEEKG DWLIYVTDA GQGTHFYIL 
      FQIARKIGL LTDNIRVDH VPFGVVLGE DKKKFKTRS 
      GDTVRLIDL LEEGLKRAL DKLKEKGRD QVLTPEELK 
      QAQESIAYG CIKYADLSH NRHHEYVFS FDKMLEDKG 
      NTAVYLLYA YTRIRSIAR TANFDDKKI EDVAKSHTI 
      SLDHEKEWK LAKVLLKFP EELDKVTKD LCPHHLCEY 
      VYEIATTFT EFYDSCYCV EKDSSGDIV KVNHGRILL 
      AEATARVMA KCLDILGLK PVAKM
    CDS sequence
    • >TC007521 RA
      atgggttta atagacgaa acggtctct ttttatgat 
      aaagaagca agttgtgcg gaagaccaa ataaaatgc 
      ctaaaaaag cagctctta caattgcaa caaaacatc 
      aagaataaa acggttgac acgtcacaa aaccctgaa 
      ctccagaaa ttggtgacg gaaaatacg aagctaaaa 
      caccgtctg accatattg aagagggct attgccagt 
      ttgagtcac tcgcaaaca tcccgtgac tccaccaga 
      atgcaaaac attcaagaa atccttgat gagattttt 
      ggggctgcc atctcgtcc gcctgccct gacatcacg 
      gacgcccct gttgtcatc accttaagt aataacgca 
      aaatttggt gattatcaa tgcaattct gctatgcaa 
      atttccaat atttacaag caattgggt aaaaaagtc 
      tcccctcga gatatcgcc aataaaatc gttgaaaac 
      gtaagtcca cacgaattg gtcgataaa ctggaagtt 
      gctgggcca gggttcatt aatgttttt gtgagtaaa 
      gcgttcggt ttgcgtaat ttatcgacg attttcacg 
      catggggtc aggccgccc ccttacaag aaattgcgc 
      gttgttgtt gatttatcg tcgccaaat atcgcgaaa 
      gaaatgcac gtggggcat ttaaggtcg acgataatc 
      ggagacacg atttgtcgc ttgttggag tttttagga 
      cacgatgtt ttgcgaatt aatcatatt ggtgattgg 
      ggaacgcag tttgggatg ttgatcgtc catttgcag 
      gagaaattt cccgattat ttgagcaaa gcgccccca 
      atttctgac ctgcaagcg ttttataaa gaatcgaaa 
      aaacggttt gacgaagat gaagacttc aagaagcgg 
      gcttatgcc ggtgttgtt aagttacag tcgggggac 
      cccaattac attaaggct tggcagttg atttgtgac 
      gtttctcgt aaggaattt caagtaatt tatgatcgt 
      ttggatgtt aagttaatt gagcgaggg gagtcgttt 
      tatcagtct cgaatgaac gacattgtt aaagaattg 
      gaagctggg ggttatttg gaagatgat gagggacgt 
      aaagtaatg tggggtgat aaaaacgat gggaatata 
      ccgtttacg gttgttaaa tcggacggt ggctacact 
      tacgacacc tctgatttg gctacgatt aaacaaaga 
      atagttgag gagaaaggc gattggttg atttatgta 
      acggatgcc gggcaaggg actcacttc tacattctg 
      ttccaaatt gctcgaaaa attggcctg ttgactgat 
      aacatccgg gttgatcat gtcccgttt ggagttgtt 
      ttgggtgaa gataaaaag aaatttaaa acccggtcc 
      ggtgacaca gttcgatta atagacctt ttagaagaa 
      ggtttaaaa cgtgctttg gataaactc aaagagaag 
      ggtcgtgat caggttttg actcctgaa gaattgaaa 
      caagcacag gagtccata gcttacggg tgtattaag 
      tacgccgat ttgagccac aaccgtcac cacgagtac 
      gtctttagt ttcgataaa atgctagag gataaggga 
      aatacggcc gtttatttg ctgtatgct tatacacgt 
      ataagatca attgcgcgt actgcaaac ttcgacgac 
      aagaaaatc gaagacgtg gcgaaaagt cacacgatt 
      tctttagac cacgagaag gagtggaaa ttggcgaaa 
      gtgttgttg aagtttcca gaggaactc gacaaagtt 
      acgaaggat ttgtgtccg caccatttg tgcgaatac 
      gtctacgaa atagcaact acctttaca gagttttac 
      gattcttgc tattgtgta gagaaggat tcatcggga 
      gatattgtc aaagtgaac catgggagg attctgctg 
      gcggaagct acagcccgt gtcatggca aagtgtctc 
      gatattttg ggccttaaa cccgtagcg aaaatgtag
    mRNA sequence
    • >TC007521 RA
      ttggcaacg gcggccatt taagttatt tttgctgca 
      cacgtgaac ttttgaatc gtttttgtt gttattttg 
      tgcaataat tcgcaattt aacgacgtg ttatgggtt 
      taatagacg aaacggtct ctttttatg ataaagaag 
      caagttgtg cggaagacc aaataaaat gcctaaaaa 
      agcagctct tacaattgc aacaaaaca tcaagaata 
      aaacggttg acacgtcac aaaaccctg aactccaga 
      aattggtga cggaaaata cgaagctaa aacaccgtc 
      tgaccatat tgaagaggg ctattgcca gtttgagtc 
      actcgcaaa catcccgtg actccacca gaatgcaaa 
      acattcaag aaatccttg atgagattt ttggggctg 
      ccatctcgt ccgcctgcc ctgacatca cggacgccc 
      ctgttgtca tcaccttaa gtaataacg caaaatttg 
      gtgattatc aatgcaatt ctgctatgc aaatttcca 
      atatttaca agcaattgg gtaaaaaag tctcccctc 
      gagatatcg ccaataaaa tcgttgaaa acgtaagtc 
      cacacgaat tggtcgata aactggaag ttgctgggc 
      cagggttca ttaatgttt ttgtgagta aagcgttcg 
      gtttgcgta atttatcga cgattttca cgcatgggg 
      tcaggccgc ccccttaca agaaattgc gcgttgttg 
      ttgatttat cgtcgccaa atatcgcga aagaaatgc 
      acgtggggc atttaaggt cgacgataa tcggagaca 
      cgatttgtc gcttgttgg agtttttag gacacgatg 
      ttttgcgaa ttaatcata ttggtgatt ggggaacgc 
      agtttggga tgttgatcg tccatttgc aggagaaat 
      ttcccgatt atttgagca aagcgcccc caatttctg 
      acctgcaag cgttttata aagaatcga aaaaacggt 
      ttgacgaag atgaagact tcaagaagc gggcttatg 
      ccggtgttg ttaagttac agtcggggg accccaatt 
      acattaagg cttggcagt tgatttgtg acgtttctc 
      gtaaggaat ttcaagtaa tttatgatc gtttggatg 
      ttaagttaa ttgagcgag gggagtcgt tttatcagt 
      ctcgaatga acgacattg ttaaagaat tggaagctg 
      ggggttatt tggaagatg atgagggac gtaaagtaa 
      tgtggggtg ataaaaacg atgggaata taccgttta 
      cggttgtta aatcggacg gtggctaca cttacgaca 
      cctctgatt tggctacga ttaaacaaa gaatagttg 
      aggagaaag gcgattggt tgatttatg taacggatg 
      ccgggcaag ggactcact tctacattc tgttccaaa 
      ttgctcgaa aaattggcc tgttgactg ataacatcc 
      gggttgatc atgtcccgt ttggagttg ttttgggtg 
      aagataaaa agaaattta aaacccggt ccggtgaca 
      cagttcgat taatagacc ttttagaag aaggtttaa 
      aacgtgctt tggataaac tcaaagaga agggtcgtg 
      atcaggttt tgactcctg aagaattga aacaagcac 
      aggagtcca tagcttacg ggtgtatta agtacgccg 
      atttgagcc acaaccgtc accacgagt acgtcttta 
      gtttcgata aaatgctag aggataagg gaaatacgg 
      ccgtttatt tgctgtatg cttatacac gtataagat 
      caattgcgc gtactgcaa acttcgacg acaagaaaa 
      tcgaagacg tggcgaaaa gtcacacga tttctttag 
      accacgaga aggagtgga aattggcga aagtgttgt 
      tgaagtttc cagaggaac tcgacaaag ttacgaagg 
      atttgtgtc cgcaccatt tgtgcgaat acgtctacg 
      aaatagcaa ctaccttta cagagtttt acgattctt 
      gctattgtg tagagaagg attcatcgg gagatattg 
      tcaaagtga accatggga ggattctgc tggcggaag 
      ctacagccc gtgtcatgg caaagtgtc tcgatattt 
      tgggcctta aacccgtag cgaaaatgt agtttcaat 
      aaatttgtt caaaatcac taaaatata ttgtccaaa 
      ccctg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY