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Gene information
General information
TC007515 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3463481..3471568
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007515
    iBeetle screen iB_01209

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 28.57% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    antenna potentially robustness decreased - Number of animals showing the phenotype: 5


    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007515 RA
      MSHHSDDNM LIATSDSFW EPGNYKKTT KRIEDGYKL 
      CTELMTLVN ERAEIEKAY AKSLKTWSK KWNDSIEKG 
      PEYGTTEAA WKGVLVEAD RRCDLHTKI RDNLTNDVI 
      NKIKVWQKD AYHKSMMNI KEKKDMDDA FKKAQKPWA 
      KLLQKVEKA KSDYHTACK TEKSAANQE RNAAGDSSF 
      SADQVKKMQ DRVQKSKEE VQKCKEKYE LALQEINQY 
      NPKYMEDMT VVFDKCQEM EAQRLNFIK GTLFDIHKY 
      LNVSQDPTL QQIYEELYH TINNADYEK DLKWWSNNH 
      GVNMAMNWP QFEDYTEEF RDIHKGKSK DSATSGITL 
      INQRPVGED LHEYSSNNK ALKAKTTVT AVKVNNTPP 
      VQKKDVVKP ATVEKVESP ANRASTITN GSSTKDANP 
      FDEEEWDES DALVDNGEP GVPVKALYD YEGAENDEL 
      SFKTGDVFE KLEDEDEQG WCKGRKDGR VGLYPANYV 
      EVVPH
    CDS sequence
    • >TC007515 RA
      atgtcgcat cacagcgat gacaacatg ctgatcgcg 
      accagcgac tccttctgg gaaccgggc aactataag 
      aaaaccacc aagaggatc gaagacggg tacaagctg 
      tgcaccgaa ctgatgacc ttagtgaac gaacgcgca 
      gagatcgaa aaggcgtac gcgaagagc ctgaagacg 
      tggtcgaag aagtggaac gactccatc gaaaagggg 
      cccgagtac gggacgacg gaagccgcc tggaagggg 
      gtcctggtc gaggccgac agacgatgc gatctccat 
      acgaaaatc agggacaat ctcaccaac gatgtgata 
      aataaaatt aaagtgtgg cagaaagat gcatatcat 
      aaatcgatg atgaatatt aaagagaag aaagacatg 
      gacgatgcg ttcaaaaaa gcgcagaaa ccatgggcg 
      aagttgctg caaaaggtc gaaaaggcc aagagtgat 
      tatcacacg gcgtgcaag acggaaaag tcggcggcc 
      aatcaggag aggaatgcg gccggcgat agctccttt 
      tcggccgac caggtgaaa aagatgcaa gaccgcgtg 
      cagaagtcg aaggaagag gtgcaaaag tgcaaggaa 
      aagtacgag ctggccctg caagagatc aaccaatac 
      aatccgaaa tacatggaa gatatgacc gtagtgttc 
      gacaaatgc caagaaatg gaagctcaa aggttgaat 
      tttatcaaa gggacgctt ttcgacatc cacaaatac 
      ctaaacgtt tcccaagac ccgacgttg caacaaatc 
      tacgaggag ttgtaccat acgattaac aacgccgac 
      tatgagaag gacctgaaa tggtggtcg aacaaccac 
      ggggtgaac atggcaatg aattggccg cagttcgag 
      gattatacc gaggaattc cgcgatata cacaagggc 
      aagtcgaag gattcggcc acgtccggc atcacgcta 
      ataaaccag aggcccgtc ggggaagac ttgcatgaa 
      tattccagc aataataaa gcattgaaa gctaaaact 
      accgtgacg gctgtcaaa gtcaacaat acgccgccg 
      gtgcagaag aaggacgtc gtcaaaccg gcgacggtt 
      gaaaaggtc gagagccct gcgaatcga gcgtcgacc 
      ataacgaac ggctcgagt acaaaagac gcgaacccc 
      ttcgatgag gaggagtgg gacgagagt gatgccctt 
      gtggacaat ggggagccg ggggttccg gtcaaggcg 
      ttgtacgac tatgagggg gcagagaac gacgaactg 
      agtttcaag acaggtgac gtatttgag aaactggaa 
      gatgaagac gagcaaggg tggtgtaag gggcgcaaa 
      gacggacga gtggggctg tacccagcc aattatgtt 
      gaagtggtg ccacattga
    mRNA sequence
    • >TC007515 RA
      tggcgccgc ggcggcagg ttcggcaac taaaagaat 
      tgaagtgga aatagttta gtgacatta gtggccgtt 
      tagaagtcg gttgtgtgg tgaatttgt aatttttat 
      ttgtcccaa gcgtttaat gtttgggag aggttgagg 
      cagttcccg tgtgcatgt tttagcccc agctcgcgg 
      tccggatac gttcttttg tttttctca tcaacctga 
      tccaggcgc ggaggccga accatgtcg catcacagc 
      gatgacaac atgctgatc gcgaccagc gactccttc 
      tgggaaccg ggcaactat aagaaaacc accaagagg 
      atcgaagac gggtacaag ctgtgcacc gaactgatg 
      accttagtg aacgaacgc gcagagatc gaaaaggcg 
      tacgcgaag agcctgaag acgtggtcg aagaagtgg 
      aacgactcc atcgaaaag gggcccgag tacgggacg 
      acggaagcc gcctggaag ggggtcctg gtcgaggcc 
      gacagacga tgcgatctc catacgaaa atcagggac 
      aatctcacc aacgatgtg ataaataaa attaaagtg 
      tggcagaaa gatgcatat cataaatcg atgatgaat 
      attaaagag aagaaagac atggacgat gcgttcaaa 
      aaagcgcag aaaccatgg gcgaagttg ctgcaaaag 
      gtcgaaaag gccaagagt gattatcac acggcgtgc 
      aagacggaa aagtcggcg gccaatcag gagaggaat 
      gcggccggc gatagctcc ttttcggcc gaccaggtg 
      aaaaagatg caagaccgc gtgcagaag tcgaaggaa 
      gaggtgcaa aagtgcaag gaaaagtac gagctggcc 
      ctgcaagag atcaaccaa tacaatccg aaatacatg 
      gaagatatg accgtagtg ttcgacaaa tgccaagaa 
      atggaagct caaaggttg aattttatc aaagggacg 
      cttttcgac atccacaaa tacctaaac gtttcccaa 
      gacccgacg ttgcaacaa atctacgag gagttgtac 
      catacgatt aacaacgcc gactatgag aaggacctg 
      aaatggtgg tcgaacaac cacggggtg aacatggca 
      atgaattgg ccgcagttc gaggattat accgaggaa 
      ttccgcgat atacacaag ggcaagtcg aaggattcg 
      gccacgtcc ggcatcacg ctaataaac cagaggccc 
      gtcggggaa gacttgcat gaatattcc agcaataat 
      aaagcattg aaagctaaa actaccgtg acggctgtc 
      aaagtcaac aatacgccg ccggtgcag aagaaggac 
      gtcgtcaaa ccggcgacg gttgaaaag gtcgagagc 
      cctgcgaat cgagcgtcg accataacg aacggctcg 
      agtacaaaa gacgcgaac cccttcgat gaggaggag 
      tgggacgag agtgatgcc cttgtggac aatggggag 
      ccgggggtt ccggtcaag gcgttgtac gactatgag 
      ggggcagag aacgacgaa ctgagtttc aagacaggt 
      gacgtattt gagaaactg gaagatgaa gacgagcaa 
      gggtggtgt aaggggcgc aaagacgga cgagtgggg 
      ctgtaccca gccaattat gttgaagtg gtgccacat 
      tgatacgtg tcaaatgtt aattgttgt tattttttg 
      aaatatata gagctattg ctcgttatg aaatataag 
      tttttgttg actggcata ggtattgcc ttacagtat 
      tgtacaaac tgatgggaa aaacagatt ataatttat 
      gatattatc aagcatttt ttcagtttg gttgtaaca 
      tttttagag gtattataa cgtaatagc gctaggcct 
      actcattac attaatgta tatacattt gattaaatc 
      agaacactt ccagtgggt acatatgat catttagtt 
      taggtatct gtaaattat tacctagaa tagagtgat 
      tataaattg tatataacg taacagtaa taaattata 
      atctatttt aaagcgact tcttgcttg acctgttaa 
      aaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY