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Gene information
General information
TC007515 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3463481..3471568
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007515
    iBeetle screen iB_01209

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 28.57% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    antenna potentially robustness decreased - Number of animals showing the phenotype: 5


    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007515 RA
      MSHHSDDNM LIATSDSFW EPGNYKKTT KRIEDGYKL 
CTELMTLVN ERAEIEKAY AKSLKTWSK KWNDSIEKG 
PEYGTTEAA WKGVLVEAD RRCDLHTKI RDNLTNDVI 
NKIKVWQKD AYHKSMMNI KEKKDMDDA FKKAQKPWA 
KLLQKVEKA KSDYHTACK TEKSAANQE RNAAGDSSF 
SADQVKKMQ DRVQKSKEE VQKCKEKYE LALQEINQY 
NPKYMEDMT VVFDKCQEM EAQRLNFIK GTLFDIHKY 
LNVSQDPTL QQIYEELYH TINNADYEK DLKWWSNNH 
GVNMAMNWP QFEDYTEEF RDIHKGKSK DSATSGITL 
INQRPVGED LHEYSSNNK ALKAKTTVT AVKVNNTPP 
VQKKDVVKP ATVEKVESP ANRASTITN GSSTKDANP 
FDEEEWDES DALVDNGEP GVPVKALYD YEGAENDEL 
SFKTGDVFE KLEDEDEQG WCKGRKDGR VGLYPANYV 
EVVPH
    CDS sequence
    • >TC007515 RA
      atgtcgcat cacagcgat gacaacatg ctgatcgcg 
accagcgac tccttctgg gaaccgggc aactataag 
aaaaccacc aagaggatc gaagacggg tacaagctg 
tgcaccgaa ctgatgacc ttagtgaac gaacgcgca 
gagatcgaa aaggcgtac gcgaagagc ctgaagacg 
tggtcgaag aagtggaac gactccatc gaaaagggg 
cccgagtac gggacgacg gaagccgcc tggaagggg 
gtcctggtc gaggccgac agacgatgc gatctccat 
acgaaaatc agggacaat ctcaccaac gatgtgata 
aataaaatt aaagtgtgg cagaaagat gcatatcat 
aaatcgatg atgaatatt aaagagaag aaagacatg 
gacgatgcg ttcaaaaaa gcgcagaaa ccatgggcg 
aagttgctg caaaaggtc gaaaaggcc aagagtgat 
tatcacacg gcgtgcaag acggaaaag tcggcggcc 
aatcaggag aggaatgcg gccggcgat agctccttt 
tcggccgac caggtgaaa aagatgcaa gaccgcgtg 
cagaagtcg aaggaagag gtgcaaaag tgcaaggaa 
aagtacgag ctggccctg caagagatc aaccaatac 
aatccgaaa tacatggaa gatatgacc gtagtgttc 
gacaaatgc caagaaatg gaagctcaa aggttgaat 
tttatcaaa gggacgctt ttcgacatc cacaaatac 
ctaaacgtt tcccaagac ccgacgttg caacaaatc 
tacgaggag ttgtaccat acgattaac aacgccgac 
tatgagaag gacctgaaa tggtggtcg aacaaccac 
ggggtgaac atggcaatg aattggccg cagttcgag 
gattatacc gaggaattc cgcgatata cacaagggc 
aagtcgaag gattcggcc acgtccggc atcacgcta 
ataaaccag aggcccgtc ggggaagac ttgcatgaa 
tattccagc aataataaa gcattgaaa gctaaaact 
accgtgacg gctgtcaaa gtcaacaat acgccgccg 
gtgcagaag aaggacgtc gtcaaaccg gcgacggtt 
gaaaaggtc gagagccct gcgaatcga gcgtcgacc 
ataacgaac ggctcgagt acaaaagac gcgaacccc 
ttcgatgag gaggagtgg gacgagagt gatgccctt 
gtggacaat ggggagccg ggggttccg gtcaaggcg 
ttgtacgac tatgagggg gcagagaac gacgaactg 
agtttcaag acaggtgac gtatttgag aaactggaa 
gatgaagac gagcaaggg tggtgtaag gggcgcaaa 
gacggacga gtggggctg tacccagcc aattatgtt 
gaagtggtg ccacattga
    mRNA sequence
    • >TC007515 RA
      tggcgccgc ggcggcagg ttcggcaac taaaagaat 
tgaagtgga aatagttta gtgacatta gtggccgtt 
tagaagtcg gttgtgtgg tgaatttgt aatttttat 
ttgtcccaa gcgtttaat gtttgggag aggttgagg 
cagttcccg tgtgcatgt tttagcccc agctcgcgg 
tccggatac gttcttttg tttttctca tcaacctga 
tccaggcgc ggaggccga accatgtcg catcacagc 
gatgacaac atgctgatc gcgaccagc gactccttc 
tgggaaccg ggcaactat aagaaaacc accaagagg 
atcgaagac gggtacaag ctgtgcacc gaactgatg 
accttagtg aacgaacgc gcagagatc gaaaaggcg 
tacgcgaag agcctgaag acgtggtcg aagaagtgg 
aacgactcc atcgaaaag gggcccgag tacgggacg 
acggaagcc gcctggaag ggggtcctg gtcgaggcc 
gacagacga tgcgatctc catacgaaa atcagggac 
aatctcacc aacgatgtg ataaataaa attaaagtg 
tggcagaaa gatgcatat cataaatcg atgatgaat 
attaaagag aagaaagac atggacgat gcgttcaaa 
aaagcgcag aaaccatgg gcgaagttg ctgcaaaag 
gtcgaaaag gccaagagt gattatcac acggcgtgc 
aagacggaa aagtcggcg gccaatcag gagaggaat 
gcggccggc gatagctcc ttttcggcc gaccaggtg 
aaaaagatg caagaccgc gtgcagaag tcgaaggaa 
gaggtgcaa aagtgcaag gaaaagtac gagctggcc 
ctgcaagag atcaaccaa tacaatccg aaatacatg 
gaagatatg accgtagtg ttcgacaaa tgccaagaa 
atggaagct caaaggttg aattttatc aaagggacg 
cttttcgac atccacaaa tacctaaac gtttcccaa 
gacccgacg ttgcaacaa atctacgag gagttgtac 
catacgatt aacaacgcc gactatgag aaggacctg 
aaatggtgg tcgaacaac cacggggtg aacatggca 
atgaattgg ccgcagttc gaggattat accgaggaa 
ttccgcgat atacacaag ggcaagtcg aaggattcg 
gccacgtcc ggcatcacg ctaataaac cagaggccc 
gtcggggaa gacttgcat gaatattcc agcaataat 
aaagcattg aaagctaaa actaccgtg acggctgtc 
aaagtcaac aatacgccg ccggtgcag aagaaggac 
gtcgtcaaa ccggcgacg gttgaaaag gtcgagagc 
cctgcgaat cgagcgtcg accataacg aacggctcg 
agtacaaaa gacgcgaac cccttcgat gaggaggag 
tgggacgag agtgatgcc cttgtggac aatggggag 
ccgggggtt ccggtcaag gcgttgtac gactatgag 
ggggcagag aacgacgaa ctgagtttc aagacaggt 
gacgtattt gagaaactg gaagatgaa gacgagcaa 
gggtggtgt aaggggcgc aaagacgga cgagtgggg 
ctgtaccca gccaattat gttgaagtg gtgccacat 
tgatacgtg tcaaatgtt aattgttgt tattttttg 
aaatatata gagctattg ctcgttatg aaatataag 
tttttgttg actggcata ggtattgcc ttacagtat 
tgtacaaac tgatgggaa aaacagatt ataatttat 
gatattatc aagcatttt ttcagtttg gttgtaaca 
tttttagag gtattataa cgtaatagc gctaggcct 
actcattac attaatgta tatacattt gattaaatc 
agaacactt ccagtgggt acatatgat catttagtt 
taggtatct gtaaattat tacctagaa tagagtgat 
tataaattg tatataacg taacagtaa taaattata 
atctatttt aaagcgact tcttgcttg acctgttaa 
aaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY