Status
Please wait ...
Gene information
General information
TC007510 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 3537860..3539728
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007510
    iBeetle screen iB_01207
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007510 RA
      MGSVNVNRS VSDVFYRYK MPRLMAKVE GKGNGIKTV 
      IVNMAEVAK ALGRPPTYP TKYFGCELG AQTQFDFKN 
      ERFIVNGSH DAGKLQDLL DGFIRKFVL CPECENPET 
      ELLVSTKRS TISQGCKAC GYHGPLESN HKLVTFILK 
      NPPNLNPAV QGSSLTEGK RSKRSKRAN GDTNGDTSQ 
      VDDQNESLE ASVNENSKN GDDDDAHEW QADVSEEAV 
      RARMQDLTE GAKNMTIND DLEKSEKDR MDIFYELVK 
      SKRDAGQLD NPQVYKELY NEADRLEIK TKAPLVLAE 
      VLFDQNMKN QLSDYRLLL LRFCHDDKK AQRYLMGGF 
      EQVIALHRD TLLNKVPVL LKTLYDLDV LSEATIIAW 
      AEKVSKKYV SKEVSQEIH DKAAPFVKW LKEAETEES 
      ESEEESDDD VEIEYNDRA QVVPLKPTS TTNNKTERV 
      EDDGEDDVD IDAI
    CDS sequence
    • >TC007510 RA
      atgggcagc gtcaacgtc aatcgcagc gtcagcgat 
      gtcttttat cgctacaag atgccccgg ctgatggcc 
      aaagtggag ggcaaaggt aacggcatc aaaacggtc 
      attgttaat atggccgaa gtggccaag gccctaggc 
      cgacccccc acctatccg acgaagtac tttggctgc 
      gagttgggc gctcagact caattcgat ttcaaaaac 
      gaaagattt atcgtcaat ggatcgcac gatgcgggc 
      aaactacaa gacctactc gatggcttc atccggaaa 
      ttcgttttg tgtcccgaa tgtgagaac cctgaaacc 
      gagctgctt gtctccacc aaacgcagc accatttct 
      caaggttgc aaggcatgt ggctaccac ggcccgcta 
      gaatccaac cataaattg gtcactttt attttgaaa 
      aatccgcca aatttaaat cctgccgtt cagggctcg 
      tcattgact gaaggtaag agatcaaag aggagcaaa 
      cgcgccaac ggagacacc aatggagac accagtcaa 
      gtcgacgat caaaacgaa agcttggag gcatcagtt 
      aatgaaaat tccaagaac ggcgatgat gacgacgca 
      cacgaatgg caagctgac gtgtcggaa gaagccgtt 
      cgcgcccga atgcaggat ttgaccgaa ggtgcaaaa 
      aatatgacc atcaatgat gatttagaa aagagcgaa 
      aaggaccga atggatatt ttctacgag ttggtcaag 
      tcgaagcgc gacgctggt cagttagac aacccacag 
      gtgtataag gagttgtac aatgaagcc gatcgtttg 
      gaaatcaag actaaggcc cctctggtc ttggccgag 
      gttctcttc gatcagaat atgaaaaac cagttgtcc 
      gattatagg ttgttgctt ttgcgtttc tgccatgat 
      gataaaaag gcacagaga tatttaatg ggagggttc 
      gagcaagtg attgccttg catcgcgat actttgctc 
      aataaggta ccggttttg ctcaaaacg ctctacgat 
      ttggatgtt ttgagcgaa gctacgatt atcgcatgg 
      gcggagaag gtgtcgaag aagtatgtg tcaaaggaa 
      gtcagtcag gagattcat gataaggcc gcgccgttt 
      gtcaagtgg ttgaaggaa gccgaaacg gaagagtcc 
      gaaagcgaa gaagaaagt gatgatgat gtcgaaatt 
      gaatacaac gaccgagct caagtcgtt ccattgaaa 
      ccgacatcc actacaaac aataagacg gaacgtgtc 
      gaagatgat ggagaggat gacgtcgac attgatgcc 
      atttaa
    mRNA sequence
    • >TC007510 RA
      catgggcag cgtcaacgt caatcgcag cgtcagcga 
      tgtctttta tcgctacaa gatgccccg gctgatggc 
      caaagtgga gggcaaagg taacggcat caaaacggt 
      cattgttaa tatggccga agtggccaa ggccctagg 
      ccgaccccc cacctatcc gacgaagta ctttggctg 
      cgagttggg cgctcagac tcaattcga tttcaaaaa 
      cgaaagatt tatcgtcaa tggatcgca cgatgcggg 
      caaactaca agacctact cgatggctt catccggaa 
      attcgtttt gtgtcccga atgtgagaa ccctgaaac 
      cgagctgct tgtctccac caaacgcag caccatttc 
      tcaaggttg caaggcatg tggctacca cggcccgct 
      agaatccaa ccataaatt ggtcacttt tattttgaa 
      aaatccgcc aaatttaaa tcctgccgt tcagggctc 
      gtcattgac tgaaggtaa gagatcaaa gaggagcaa 
      acgcgccaa cggagacac caatggaga caccagtca 
      agtcgacga tcaaaacga aagcttgga ggcatcagt 
      taatgaaaa ttccaagaa cggcgatga tgacgacgc 
      acacgaatg gcaagctga cgtgtcgga agaagccgt 
      tcgcgcccg aatgcagga tttgaccga aggtgcaaa 
      aaatatgac catcaatga tgatttaga aaagagcga 
      aaaggaccg aatggatat tttctacga gttggtcaa 
      gtcgaagcg cgacgctgg tcagttaga caacccaca 
      ggtgtataa ggagttgta caatgaagc cgatcgttt 
      ggaaatcaa gactaaggc ccctctggt cttggccga 
      ggttctctt cgatcagaa tatgaaaaa ccagttgtc 
      cgattatag gttgttgct tttgcgttt ctgccatga 
      tgataaaaa ggcacagag atatttaat gggagggtt 
      cgagcaagt gattgcctt gcatcgcga tactttgct 
      caataaggt accggtttt gctcaaaac gctctacga 
      tttggatgt tttgagcga agctacgat tatcgcatg 
      ggcggagaa ggtgtcgaa gaagtatgt gtcaaagga 
      agtcagtca ggagattca tgataaggc cgcgccgtt 
      tgtcaagtg gttgaagga agccgaaac ggaagagtc 
      cgaaagcga agaagaaag tgatgatga tgtcgaaat 
      tgaatacaa cgaccgagc tcaagtcgt tccattgaa 
      accgacatc cactacaaa caataagac ggaacgtgt 
      cgaagatga tggagagga tgacgtcga cattgatgc 
      catttaaag agttcttat cccgataac agggtgccg 
      agtgtaatt ttttttggt tcgggttca cgcactcgg 
      cttttttgt ctcaaaaat gattcgcat tatttttat 
      catcaaatg gttgtatgt ttagcaagt aaagtttga 
      acacatttt gttatcaga tagttacga aactgtttt 
      ctggtaccc gacattaat gtaacaaaa cttccattc 
      acgaataaa ttgattgat tgtatcatt tctaaggtt 
      tcattcaag tgtttttct tttcacaat attatatga 
      gatgttata taaaggatt ttattataa ttactatta 
      tagttatat agataggtt tccatattt cccatgtca 
      aatatcgag ctgtatccg tgggtttta cgattttat 
      tattgttta aatgtgaat aaaatttaa agcatcac