Status
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Gene information
General information
TC032792
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007503,TC007505
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3557586..3642343
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032792
    iBeetle screen iB_01205

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    procephalic head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032792 RA
      MPGEEELHL NGVGRTQIY QPSTGEKHV LFYPTSPAP 
      SVNSDERRS WSQASSSLA TQTRSNHRN SNNNADTGA 
      RMKTSEAAP QPDAGAHPS DPNDGFRAR YNFRIGIYG 
      WRKRCLYIL ILVLLVMVI VNLALTLWV LKVMEFSSE 
      GMGQLKIVS GGLRLEGKA FVLDTLIAS SIKSRTGQP 
      IIVESSRNL TLASRSKNG LLSNMIFLG DDRFECLTS 
      TFKVMDDRG VVLFAADPR EVVVGAESL RVTGEGGAS 
      FSGSVQTTL VRAEPGNDL RLESPTRSL EIHGPQGVH 
      MESRGGKMK IVSLNDISF KSEVGSIHL DASSILMPM 
      LPTAIPTSK PAPNKNQIF QVCVCNNGR LFLVPSHLH 
      CAAEEDDRI CR
    CDS sequence
    • >TC032792 RA
      atgccgggc gaagaagag ctgcacctc aacggcgtc 
      ggccggacc caaatctac cagccctcc acgggggaa 
      aagcacgtc ttgttctac cccacaagt ccggcgccg 
      tcggtcaat tcggacgag aggaggagc tggtcgcag 
      gcctcgtcc tccctagcc actcaaacg cggagcaat 
      cacagaaat tccaataat aacgcagat accggcgct 
      agaatgaag acaagtgaa gcggcgcca caacccgac 
      gcgggggcg cacccttcg gaccccaac gacggcttc 
      cgagccaga tataacttc aggattgga atttatgga 
      tggaggaag cgatgtctt tatatcctc atccttgtg 
      ctgctcgtg atggtgatt gtcaatttg gccctcacg 
      ctgtgggtc ctcaaagtt atggaattc tcctcggaa 
      ggcatgggt caactcaaa atcgtaagc ggaggatta 
      cgactcgag ggcaaagct ttcgtcttg gacactctg 
      atcgccagc agcatcaaa tccaggacc ggacagccg 
      ataattgtc gagtcgagc agaaacctg acgctggcg 
      tccaggagc aagaacgga ctgctcagc aacatgata 
      ttcctaggg gacgaccgc ttcgagtgc ctgacgagc 
      accttcaag gtgatggac gaccgagga gtggtgctg 
      tttgcggcg gacccgcgc gaagtggtc gtgggggcg 
      gaaagtctg cgggtgacg ggcgaaggg ggcgcctct 
      ttcagcggc tcggtgcag acgacgctg gtgcgggcc 
      gagcccggc aacgacttg cgcttggag tcgccgacc 
      cgctcgctg gagatccac ggcccccag ggggtgcac 
      atggagtcc cgcggcggc aaaatgaag atcgtgtcc 
      ttgaacgac atctcgttc aaatcggag gtgggctcc 
      atccacctg gacgccagc tcgatcctt atgccgatg 
      ctgcccacc gccatcccc acctccaag cccgccccc 
      aacaaaaac caaattttc caagtgtgt gtgtgcaac 
      aacggccgc ctgttcctc gtcccgtct cacctgcac 
      tgtgccgca gaggaagac gacagaatc tgccggtaa
    mRNA sequence
    • >TC032792 RA
      aatcgcgct cagtccgac gcggacgtg gagagcgtc 
      gccggcgtc gtctccaga cgcaatgtc gcgatcgca 
      ataacagtc gcgcgatgc cgggcgaag aagagctgc 
      acctcaacg gcgtcggcc ggacccaaa tctaccagc 
      cctccacgg gggaaaagc acgtcttgt tctacccca 
      caagtccgg cgccgtcgg tcaattcgg acgagagga 
      ggagctggt cgcaggcct cgtcctccc tagccactc 
      aaacgcgga gcaatcaca gaaattcca ataataacg 
      cagataccg gcgctagaa tgaagacaa gtgaagcgg 
      cgccacaac ccgacgcgg gggcgcacc cttcggacc 
      ccaacgacg gcttccgag ccagatata acttcagga 
      ttggaattt atggatgga ggaagcgat gtctttata 
      tcctcatcc ttgtgctgc tcgtgatgg tgattgtca 
      atttggccc tcacgctgt gggtcctca aagttatgg 
      aattctcct cggaaggca tgggtcaac tcaaaatcg 
      taagcggag gattacgac tcgagggca aagctttcg 
      tcttggaca ctctgatcg ccagcagca tcaaatcca 
      ggaccggac agccgataa ttgtcgagt cgagcagaa 
      acctgacgc tggcgtcca ggagcaaga acggactgc 
      tcagcaaca tgatattcc taggggacg accgcttcg 
      agtgcctga cgagcacct tcaaggtga tggacgacc 
      gaggagtgg tgctgtttg cggcggacc cgcgcgaag 
      tggtcgtgg gggcggaaa gtctgcggg tgacgggcg 
      aagggggcg cctctttca gcggctcgg tgcagacga 
      cgctggtgc gggccgagc ccggcaacg acttgcgct 
      tggagtcgc cgacccgct cgctggaga tccacggcc 
      cccaggggg tgcacatgg agtcccgcg gcggcaaaa 
      tgaagatcg tgtccttga acgacatct cgttcaaat 
      cggaggtgg gctccatcc acctggacg ccagctcga 
      tccttatgc cgatgctgc ccaccgcca tccccacct 
      ccaagcccg cccccaaca aaaaccaaa ttttccaag 
      tgtgtgtgt gcaacaacg gccgcctgt tcctcgtcc 
      cgtctcacc tgcactgtg ccgcagagg aagacgaca 
      gaatctgcc ggtaatcgg gccgacaca ccgtgtaca 
      tagactgta taaaaagcg ccaaattgt tcatattgt 
      acatatttt gtatatgaa tagggtctg ggtatagga 
      cggttcggt taagttttc tatttattt acagctaga 
      tcgagccta atgtgaaat gtttagaaa ataataaat 
      tatttctgt ttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY