Status
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Gene information
General information
TC007500 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3695743..3702203
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007500
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01204

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007500 RA
      MDTVHEENS PAPQVPQSS LAMETLDEE DASRSSSRR 
      PSAIIAALR RPSQAIALS AAHAVMNQR RYLLGLFHN 
      QSDRSISEK DATDHKEEF LKKQNRRMG DDALTVILS 
      ALYAKLLVV LGMAFPITE IISKDVRPF FYQGFYLYL 
      YLGSITFVA YMYATFVRE KAVNNIIKS YHKNDKPTF 
      PFPRMSTSV HNKPAKYGS FYLRLGAIA FGIGSMVYS 
      GLEFGRYFE LKNNHECFS TMLQAITPA TRMLLTLVQ 
      VQFIFLNTK NVDLNRHKI IARFGLMHM VAANLCEWL 
      YVLVEETKH EIIHLGEQG HNSTNSSLQ AKFCQEGQI 
      MGSLVRNAS PFLFPCTIE YSLICAVIL FEMWKRVKS 
      TEVKAETEK AGHRDDKVA THYTFNPFG GSPVNSNHH 
      FSVDCSNAH RGLFAGIMI IVLTIISLI MFFVLANEP 
      SGSEEDNLS MAEFEVNIV ELVLYILTT IVVIVAMVQ 
      MRSLKYDRK IGVEGQAGI GLDNTLLVV AQTGMFIYC 
      MFSIIGCYF TMSGSLPTG LLAEIFSFI QTCLQTMFV 
      LDGWWRRCR TLEHIKNKP GKELITFLI VANMAMWTI 
      NTLEKNRAE FRPTHLLFF GEWAWTIIT HISMPMAIF 
      YRFHSTICL FEIWKTAYK VKTQYKTPM FSVISNQ
    CDS sequence
    • >TC007500 RA
      atggacacc gtgcacgag gaaaacagc cccgcgccc 
      caagtgccc caaagcagc ctcgcaatg gagaccctg 
      gacgaggag gacgcctcg cggtcgtcg tccaggagg 
      ccgtccgcc atcatcgcg gccctgcgg cggccctcg 
      caggcgatt gccctgtcg gccgcccac gccgtcatg 
      aaccagagg cgctacctc ctggggctg ttccacaac 
      cagagcgac cgctcgatt tcggagaaa gacgccact 
      gatcacaag gaggagttt ctaaagaag cagaatcgc 
      aggatggga gacgacgct ttgacggta attctctcg 
      gcgctgtac gcaaagctg ctggtcgtc ttgggaatg 
      gcttttccg attaccgaa atcatatcg aaggacgtt 
      cggccgttt ttctaccag ggattttat ttgtatttg 
      tatttgggg agcattacc ttcgttgcg tacatgtat 
      gcgactttt gtgagagag aaggcggtt aataacatc 
      atcaagtcg tatcataaa aacgacaaa cctacgttc 
      ccgtttccc cgcatgtcc acatcggtg cacaacaag 
      ccggccaaa tacggaagc ttctattta cgccttggt 
      gcaatcgct ttcggtatc ggcagcatg gtctattcc 
      ggactagaa ttcggccga tatttcgag ctaaagaac 
      aatcacgag tgcttttcc accatgctc caggccatc 
      acgccggca accagaatg cttctgacg ctcgttcaa 
      gtgcaattc atattccta aacacgaag aacgtcgat 
      ctgaacaga cataagatt attgcccgc tttgggctt 
      atgcacatg gtggcggcc aatctgtgc gagtggctg 
      tacgtcctg gtcgaggag accaagcac gagatcata 
      cacctgggg gagcaagga cacaactcc accaattcg 
      agtctacaa gccaagttc tgccaagag gggcaaatc 
      atgggctcg ctagtgcgg aacgccagc ccgttccta 
      ttcccctgc accatagaa tacagtctg atatgtgcg 
      gtgattctt ttcgagatg tggaaacgg gtcaagtcg 
      acggaagtc aaggcggag accgagaag gcgggccac 
      cgggacgat aaggtcgcc actcattac actttcaat 
      cccttcggc ggcagcccc gtcaactct aatcaccat 
      ttcagcgtg gactgttct aacgctcac cgcggctta 
      tttgccggg attatgatc atcgttctg accataatt 
      tctttgata atgttcttc gtgttggct aacgaaccg 
      agcggcagt gaagaggat aatctgagc atggccgag 
      ttcgaagtg aacatagtc gagttggtc ctttacata 
      ttaaccacg atcgtggtc atcgtagcg atggtgcag 
      atgcgctcg ctcaaatac gaccgaaaa atcggagtt 
      gaaggccaa gcggggatc ggcctggac aacaccctc 
      ctggtcgtt gcccaaacc gggatgttc atctactgc 
      atgttttcc atcatcggg tgctacttc accatgtcc 
      gggagtctt cccacggga ctcctggcc gagatcttt 
      tcgttcatc cagacctgc ctccagacc atgttcgtg 
      ctcgatggg tggtggcgg cgctgccgt accctggag 
      catatcaag aacaagccc ggcaaggaa ctcatcacg 
      ttcctcatt gtggcaaac atggccatg tggactatc 
      aatacgttg gagaaaaac cgcgcagag tttcgacct 
      acacatttg ctcttcttc ggagagtgg gcgtggaca 
      atcatcacg cacatttcc atgcccatg gccatcttc 
      tacaggttt cattccacc atttgtttg ttcgaaata 
      tggaaaaca gcgtacaaa gttaagacg cagtataaa 
      acgccgatg ttttctgtc atatccaat caataa
    mRNA sequence
    • >TC007500 RA
      tatttcaaa aatgtgcga ataataaaa taaaaaatt 
      aactgtaac gataataaa tctagcccc acgtcactg 
      tccaaacaa tagcaatga ccaattata accagtctc 
      tcaatcttg atgtgctta ttctcatcc caaaccttt 
      gaaattccg tgatgctcc aaggccgac acctttgtt 
      aattaaatt gtttgaaca aaagtgttt gtacttcag 
      ccagcaata gtcataact cgtcggcgc gaagcacaa 
      gacgcaaaa attccctct tatcggaat ctttcagcc 
      ccgctgcag ctagaatag gtgcatgtt ttctgagtt 
      atttccaca ataactacg acttttcca actttaaat 
      ccgcgagat tataggcca ccggggagc cgtaattag 
      atggacacc gtgcacgag gaaaacagc cccgcgccc 
      caagtgccc caaagcagc ctcgcaatg gagaccctg 
      gacgaggag gacgcctcg cggtcgtcg tccaggagg 
      ccgtccgcc atcatcgcg gccctgcgg cggccctcg 
      caggcgatt gccctgtcg gccgcccac gccgtcatg 
      aaccagagg cgctacctc ctggggctg ttccacaac 
      cagagcgac cgctcgatt tcggagaaa gacgccact 
      gatcacaag gaggagttt ctaaagaag cagaatcgc 
      aggatggga gacgacgct ttgacggta attctctcg 
      gcgctgtac gcaaagctg ctggtcgtc ttgggaatg 
      gcttttccg attaccgaa atcatatcg aaggacgtt 
      cggccgttt ttctaccag ggattttat ttgtatttg 
      tatttgggg agcattacc ttcgttgcg tacatgtat 
      gcgactttt gtgagagag aaggcggtt aataacatc 
      atcaagtcg tatcataaa aacgacaaa cctacgttc 
      ccgtttccc cgcatgtcc acatcggtg cacaacaag 
      ccggccaaa tacggaagc ttctattta cgccttggt 
      gcaatcgct ttcggtatc ggcagcatg gtctattcc 
      ggactagaa ttcggccga tatttcgag ctaaagaac 
      aatcacgag tgcttttcc accatgctc caggccatc 
      acgccggca accagaatg cttctgacg ctcgttcaa 
      gtgcaattc atattccta aacacgaag aacgtcgat 
      ctgaacaga cataagatt attgcccgc tttgggctt 
      atgcacatg gtggcggcc aatctgtgc gagtggctg 
      tacgtcctg gtcgaggag accaagcac gagatcata 
      cacctgggg gagcaagga cacaactcc accaattcg 
      agtctacaa gccaagttc tgccaagag gggcaaatc 
      atgggctcg ctagtgcgg aacgccagc ccgttccta 
      ttcccctgc accatagaa tacagtctg atatgtgcg 
      gtgattctt ttcgagatg tggaaacgg gtcaagtcg 
      acggaagtc aaggcggag accgagaag gcgggccac 
      cgggacgat aaggtcgcc actcattac actttcaat 
      cccttcggc ggcagcccc gtcaactct aatcaccat 
      ttcagcgtg gactgttct aacgctcac cgcggctta 
      tttgccggg attatgatc atcgttctg accataatt 
      tctttgata atgttcttc gtgttggct aacgaaccg 
      agcggcagt gaagaggat aatctgagc atggccgag 
      ttcgaagtg aacatagtc gagttggtc ctttacata 
      ttaaccacg atcgtggtc atcgtagcg atggtgcag 
      atgcgctcg ctcaaatac gaccgaaaa atcggagtt 
      gaaggccaa gcggggatc ggcctggac aacaccctc 
      ctggtcgtt gcccaaacc gggatgttc atctactgc 
      atgttttcc atcatcggg tgctacttc accatgtcc 
      gggagtctt cccacggga ctcctggcc gagatcttt 
      tcgttcatc cagacctgc ctccagacc atgttcgtg 
      ctcgatggg tggtggcgg cgctgccgt accctggag 
      catatcaag aacaagccc ggcaaggaa ctcatcacg 
      ttcctcatt gtggcaaac atggccatg tggactatc 
      aatacgttg gagaaaaac cgcgcagag tttcgacct 
      acacatttg ctcttcttc ggagagtgg gcgtggaca 
      atcatcacg cacatttcc atgcccatg gccatcttc 
      tacaggttt cattccacc atttgtttg ttcgaaata 
      tggaaaaca gcgtacaaa gttaagacg cagtataaa 
      acgccgatg ttttctgtc atatccaat caataaggg 
      tgatgtaaa taaagatga gaaaaagaa
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