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Gene information
General information
TC007497 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 3714306..3716513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007497
    iBeetle screen iB_01203
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007497 RA
      MDEEYDAIV LGTGLKECI LSGMLSVSG KKVLHVDRN 
      KYYGGESAS ICPLEELFS KFGAPAPDE SYGRGRDWN 
      VDLIPKFLM ANGQLVKLL IHTGVTRYL EFKSVEGSY 
      VYKGGKISK VPVDQKEAL ASDLMGMFE KRRFRNFLI 
      YVQDFQQED PKTWKDFDP NTADMQALY DKFGLDKNT 
      QDFTGHALA LFRDDDYLK RPALETINR IKLYSDSLA 
      RYGKSPYLY PMYGLGELP QGFARLSAI YGGTYMLDK 
      PIDEIVLGE GGRVVGVRS GTEVAKCKQ VYCDPTYVP 
      DRVRKKGRV VRCICLMDH PIANTKDAL STQIIIPQK 
      QVGRNSDIY VSLVSYTHQ VAAKGWFVA MVSTTVETD 
      NPEIEIKPG LDLLGSIRQ KFISVSDYY EPTDDGLQS 
      QIFISQSYD ATTHFETTC LDVLDIFKR GTGEEFDFS 
      KIKHELGDE EQ
    CDS sequence
    • >TC007497 RA
      atggatgaa gaatacgat gccatcgtc ctgggcacc 
      ggcctaaaa gagtgtatt ttgtcgggt atgttgtcc 
      gtgtccggc aagaaggtg ctccacgta gaccggaac 
      aagtactac gggggtgag tcggcatcc atctgcccc 
      ttggaggag ctgttctcc aagttcggg gcgccggcc 
      cctgacgag tcctacggc cggggccgc gactggaac 
      gtggatctc atcccgaag ttcctgatg gccaacggg 
      cagttggtc aagttgctc atccacacg ggcgttact 
      cgctatctg gagttcaag tcggtggaa gggagctac 
      gtgtacaaa gggggcaag atctcgaag gtcccggtg 
      gaccagaag gaggcgctg gcgtcggac ctcatgggg 
      atgttcgag aagaggcgg ttcaggaac ttcctgatc 
      tacgtgcag gatttccag caggaggac ccgaagact 
      tggaaggat ttcgatccc aatacggcc gatatgcaa 
      gcgctgtat gataagttc gggttagat aagaatacg 
      caggatttt acagggcac gcgttggct ctgttcagg 
      gatgacgat tacttgaag aggcctgcg ttggagacc 
      atcaacagg attaagctg tattcggat tccctggcc 
      agatacggc aagtcgccg tatttgtac cccatgtac 
      gggcttgga gaactgcca caaggattc gcgaggctg 
      tccgccatc tatgggggc acctacatg ctggacaag 
      cccatcgac gagatcgtc ctgggtgag gggggcaga 
      gtcgtgggg gtgcgttcg ggcaccgag gtggccaaa 
      tgcaagcag gtctactgc gaccccact tacgtgccc 
      gaccgagtg cgcaagaag gggcgcgtg gtccgctgc 
      atctgtctc atggaccac ccgattgcc aacacgaaa 
      gacgctctc tccacccaa atcatcatt ccacagaag 
      caagtcggc cgaaactcc gacatctac gtgtcgttg 
      gtcagctac acgcaccag gtggctgcc aaaggctgg 
      tttgtggcg atggtgtcc accaccgtc gagaccgac 
      aatcccgaa atcgagatt aagcccggc ttggacctt 
      ttgggttcg atccgtcag aagttcatc tcggtttcg 
      gattattac gagccgact gatgacggg ctgcagtcg 
      cagattttc atttcgcag tcgtatgat gcaacaact 
      catttcgag acgacttgt ttggatgtt ttggatatt 
      ttcaagcgt ggcacgggc gaggagttt gatttttcg 
      aagattaag cacgagttg ggcgatgag gaacaataa
    mRNA sequence
    • >TC007497 RA
      tgcggtttt gttggcgcc cgtctgtca tagcccatt 
      gacgtgacg tatcacttc gttaattct tctctccgt 
      attatcaat aaaccagtg aaaaatgga tgaagaata 
      cgatgccat cgtcctggg caccggcct aaaagagtg 
      tattttgtc gggtatgtt gtccgtgtc cggcaagaa 
      ggtgctcca cgtagaccg gaacaagta ctacggggg 
      tgagtcggc atccatctg ccccttgga ggagctgtt 
      ctccaagtt cggggcgcc ggcccctga cgagtccta 
      cggccgggg ccgcgactg gaacgtgga tctcatccc 
      gaagttcct gatggccaa cgggcagtt ggtcaagtt 
      gctcatcca cacgggcgt tactcgcta tctggagtt 
      caagtcggt ggaagggag ctacgtgta caaaggggg 
      caagatctc gaaggtccc ggtggacca gaaggaggc 
      gctggcgtc ggacctcat ggggatgtt cgagaagag 
      gcggttcag gaacttcct gatctacgt gcaggattt 
      ccagcagga ggacccgaa gacttggaa ggatttcga 
      tcccaatac ggccgatat gcaagcgct gtatgataa 
      gttcgggtt agataagaa tacgcagga ttttacagg 
      gcacgcgtt ggctctgtt cagggatga cgattactt 
      gaagaggcc tgcgttgga gaccatcaa caggattaa 
      gctgtattc ggattccct ggccagata cggcaagtc 
      gccgtattt gtaccccat gtacgggct tggagaact 
      gccacaagg attcgcgag gctgtccgc catctatgg 
      gggcaccta catgctgga caagcccat cgacgagat 
      cgtcctggg tgagggggg cagagtcgt gggggtgcg 
      ttcgggcac cgaggtggc caaatgcaa gcaggtcta 
      ctgcgaccc cacttacgt gcccgaccg agtgcgcaa 
      gaaggggcg cgtggtccg ctgcatctg tctcatgga 
      ccacccgat tgccaacac gaaagacgc tctctccac 
      ccaaatcat cattccaca gaagcaagt cggccgaaa 
      ctccgacat ctacgtgtc gttggtcag ctacacgca 
      ccaggtggc tgccaaagg ctggtttgt ggcgatggt 
      gtccaccac cgtcgagac cgacaatcc cgaaatcga 
      gattaagcc cggcttgga ccttttggg ttcgatccg 
      tcagaagtt catctcggt ttcggatta ttacgagcc 
      gactgatga cgggctgca gtcgcagat tttcatttc 
      gcagtcgta tgatgcaac aactcattt cgagacgac 
      ttgtttgga tgttttgga tattttcaa gcgtggcac 
      gggcgagga gtttgattt ttcgaagat taagcacga 
      gttgggcga tgaggaaca ataatggat ttggttgag 
      tttggttaa gagtaggta ttctatgtg caagttttt 
      tttatatgg ttctgtttt gtttacacc gctgcattg 
      atactctac ttgctatac aaaataatt ccgtggctt 
      gggcccaag cacaattaa tttgtatat acagtaaaa 
      atttatacc ttagtgaat aaaataatt gcttaattt 
      taaggatcc tgatgtgca aactaaagc attagcttg 
      actataaaa acgagaatt gttagtaat gaatttttt 
      tatttgcaa aatggggtt ttattgtaa ctcattatg 
      tcgaaaagt gtatttata gctttatgt tctattaga 
      tatgcatgt ctgacataa tctttggtg atatggcac 
      attgatatg tattatgtg atggatcaa atataccaa 
      aaatacgga aattttgtg tattgaatc actgagctt 
      cgcattcca tattatcat aggttttaa tgttcacga 
      aatgttaat agatcgtgt tttgtgaaa attatcgtc 
      gcagtattt ctgctattc gtcctcatc acattacgc 
      tatccagtc cctatctcg tagtaggaa tactgtgaa 
      ctcttgttg taaaatatt tacaaaaag aaataaaaa 
      tcttcaaaa gggaaacac ggacttttc ctacacctc 
      acaaac