Status
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Gene information
General information
TC007480 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3893459..3895153
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007480
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01201

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    pupal molt not completely molted - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007480 RA
      MDHLAQDLS VALEESESC GPITFAHET PGKWGMRRR 
TRSAGNLLV YMNNSPDNQ AEDSSSSNS ETRTEKTSS 
SFEWRLKHA LQSYIRYSR RCKLGVNHS LESDSVNEN 
SPARPNLRR KRKLKRMSI DEAPVPPCG KRKRPQRFD 
IVENGRSLR HPTRIRPLH QTVVDKIEK MFQNSSNLD 
DTNTMETQS IDDCDFTSE SSISWSGGE GHDGDDELT 
DWAPTMDNP SSMEQPFNE ADPREIRAG CRRLGDERP 
GFSISTGAN ERVAKFLQD TTKSELRVL GGEREKLGQ 
LAALYSLDL WFESPTSSL LRKTNKTPC MQPPQKHHS 
GMSAGHKRI RTHGRFVGV E
    CDS sequence
    • >TC007480 RA
      atggatcat ctcgcccaa gatttgagc gtggctcta 
gaagaatcc gaaagttgt ggtcccata actttcgct 
catgaaacc cccggaaaa tggggcatg agacgtcgc 
actcgctcc gcggggaac ttactagtt tacatgaac 
aattccccc gataatcaa gcagaagac agttccagc 
agtaattcc gagactcga actgagaag acttcaagt 
agcttcgaa tggcggtta aaacacgcc ttacagagt 
tatatcagg tacagtagg cggtgtaag ttgggggtg 
aaccacagt ttagagagc gactcagtg aatgaaaat 
tcgccagca aggccaaat ttgcgccga aaacgcaaa 
cttaaacgc atgagtatt gacgaagcg ccggtgccc 
ccgtgtggc aagcgcaag cgcccccag agattcgac 
attgtcgaa aatgggcgc tctttgcgg cacccgact 
cgaatccgc cctttgcac caaactgtc gtcgacaaa 
attgagaaa atgttccag aattcctca aatctggac 
gacaccaac accatggag acgcagagc atcgacgac 
tgcgacttc accagcgaa agcagcata agttggagc 
gggggcgaa ggccacgac ggtgacgat gaactcaca 
gactgggcc cccacgatg gacaacccc tcttccatg 
gagcagccg ttcaatgag gccgacccg cgtgaaatt 
cgtgccggt tgcaggcgg ttaggcgac gaaaggccg 
ggctttagt atcagcacg ggggcgaac gaacgcgtc 
gctaagttt ctccaagac accaccaag tccgagttg 
agggtctta gggggcgaa cgcgagaaa ttgggacag 
ttggcagct ctctactcg ttggatttg tggtttgag 
agccccacc tcttcacta ttgaggaag actaacaag 
actccttgt atgcagccc ccacagaag caccacagc 
ggcatgagc gccgggcat aagaggatt cggacgcat 
gggagattc gtaggagtg gagtag
    mRNA sequence
    • >TC007480 RA
      tttgacagt gtggtcgtg actcgttta ttgtgttta 
ttttcgtta aaatttggg caaaaactt ggcaatttt 
aaacacaaa gacgatgta gtgggccct tgggtaaaa 
tctcgtgtg tgaatcaaa tcaaaaggc tgcttcaaa 
gttatggat catctcgcc caagatttg agcgtggct 
ctagaagaa tccgaaagt tgtggtccc ataactttc 
gctcatgaa acccccgga aaatggggc atgagacgt 
cgcactcgc tccgcgggg aacttacta gtttacatg 
aacaattcc cccgataat caagcagaa gacagttcc 
agcagtaat tccgagact cgaactgag aagacttca 
agtagcttc gaatggcgg ttaaaacac gccttacag 
agttatatc aggtacagt aggcggtgt aagttgggg 
gtgaaccac agtttagag agcgactca gtgaatgaa 
aattcgcca gcaaggcca aatttgcgc cgaaaacgc 
aaacttaaa cgcatgagt attgacgaa gcgccggtg 
cccccgtgt ggcaagcgc aagcgcccc cagagattc 
gacattgtc gaaaatggg cgctctttg cggcacccg 
actcgaatc cgccctttg caccaaact gtcgtcgac 
aaaattgag aaaatgttc cagaattcc tcaaatctg 
gacgacacc aacaccatg gagacgcag agcatcgac 
gactgcgac ttcaccagc gaaagcagc ataagttgg 
agcgggggc gaaggccac gacggtgac gatgaactc 
acagactgg gcccccacg atggacaac ccctcttcc 
atggagcag ccgttcaat gaggccgac ccgcgtgaa 
attcgtgcc ggttgcagg cggttaggc gacgaaagg 
ccgggcttt agtatcagc acgggggcg aacgaacgc 
gtcgctaag tttctccaa gacaccacc aagtccgag 
ttgagggtc ttagggggc gaacgcgag aaattggga 
cagttggca gctctctac tcgttggat ttgtggttt 
gagagcccc acctcttca ctattgagg aagactaac 
aagactcct tgtatgcag cccccacag aagcaccac 
agcggcatg agcgccggg cataagagg attcggacg 
catgggaga ttcgtagga gtggagtag tgccttgga 
ggcttgatg tgaccccac tttttttag gataatttt 
aattttgtt gccgctttt tattaggga attcaagta 
attgtaggt aatgtgggg ttgtgtcac aatatcgga 
gtttgtgtg cgtttttat cattccata tcagtagat 
tcagaaaac tgtccacaa atttcgtca ttttatttt 
aattagaag ctcaatttc gacatgtta aactagtac 
aaagtatac taagtaata atgattgta cagtattta 
tttttactg taataatta ttgtatagg attaatata 
aaaaaaagt tacatattt gtcgcctta attttgagg 
attgaaata aaattcgat aatttta

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY