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Gene information
General information
TC032798
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007471 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4015155..4016744
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032798
    iBeetle screen iB_01199

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Pupal morphology
    elytra proximal blistered - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032798 RA
      MANTKPEQV DESLEDSSP EEEEAEQKT PNKFLDFTN 
VLATLQSQR NTDKLGQLP PAVKRRIKA LKKLQLETT 
NIEAKFFAE VHALECKYH KLYVPMYEK RNTIVTGAY 
EPTEEESQW PSDDEDETL GNQLKNKAK IEEEKTPEI 
IPKNPETND VPDPTGIPN FWLTIFQNV NLLSEMIQP 
PDEPILSHL QDIKVDCSE SPMSFTLSF HFSSNPYFT 
NSVLTKEYF MKCEPEEDD PFNFEGPEI YKCKGCTIN 
WNKGMNVTL KTVKKKQKH KSRGVVRTV TKTVQNDSF 
FNFFAPPEL PENAKGEEI DDELRQLLT TDFEIGHYI 
RERIVPRAV LFYTGEAIE DEDEDYEDD DDLEDTTEE 
SDSDKEEND KAARDANCK QQ
    CDS sequence
    • >TC032798 RA
      atggcaaat acaaaaccg gagcaagtg gacgagtcg 
ctggaggac tcctcgccc gaggaggag gaggccgag 
cagaagacc cccaacaag tttctcgac ttcaccaac 
gtcttggcc acgctgcag agccagcgg aacaccgac 
aagctgggg cagctgccc ccggccgtc aagcggcgg 
atcaaagcc ctgaagaag ctccagctg gagacgacc 
aacatcgag gccaagttc ttcgctgag gtgcacgcg 
ctcgaatgt aaataccac aaattgtac gtgcccatg 
tacgaaaag aggaacact atcgtgacg ggggcttac 
gagcccacg gaggaggaa tctcaatgg ccttcggac 
gacgaagac gaaacgctg gggaaccag ctcaagaac 
aaggccaag atcgaggag gagaaaacg ccggagatt 
atacccaaa aaccccgaa actaacgat gtgcccgac 
cccacggga atacccaac ttctggttg accatcttc 
cagaacgtt aaccttcta tccgagatg attcagccc 
ccagacgaa cccatcctt agccatctg caagacatc 
aaagtcgac tgttccgaa agcccaatg agcttcact 
ctatcattc cacttctcc tccaacccc tatttcacg 
aattcggtc ctcacgaaa gaatacttc atgaagtgc 
gagccggaa gaggacgat ccgtttaat ttcgagggc 
ccggaaatt tacaagtgc aagggttgc accattaac 
tggaacaag ggtatgaac gtgacgctg aaaactgtg 
aagaagaag cagaagcat aagagccgc ggtgtggtc 
cgtactgtg accaagacc gttcagaac gactcgttc 
ttcaatttc tttgcgccg cctgaactc cccgagaac 
gccaaaggg gaggaaatc gacgacgag ttgcgccag 
ttactcact actgacttt gagattggg cattacatt 
agggagaga attgttcca agggccgtg ttgttctac 
acaggggaa gcgattgaa gatgaggat gaggattac 
gaggatgac gatgatctg gaggacacc actgaagaa 
agcgattcc gataaagag gaaaacgat aaggcggcc 
agagacgcg aattgcaaa caacaataa
    mRNA sequence
    • >TC032798 RA
      gccggtcca aattcaaac gctgcgcag ctgcaggaa 
atcgtcttt gtagcgacg tagctggtg tcaaatttt 
ctctttctc gtcgttgcc gttgcctgt gtcccccgt 
cttcgtatt ccagcatca aacagccgg caaaagtaa 
aatggcaaa tacaaaacc ggagcaagt ggacgagtc 
gctggagga ctcctcgcc cgaggagga ggaggccga 
gcagaagac ccccaacaa gtttctcga cttcaccaa 
cgtcttggc cacgctgca gagccagcg gaacaccga 
caagctggg gcagctgcc cccggccgt caagcggcg 
gatcaaagc cctgaagaa gctccagct ggagacgac 
caacatcga ggccaagtt cttcgctga ggtgcacgc 
gctcgaatg taaatacca caaattgta cgtgcccat 
gtacgaaaa gaggaacac tatcgtgac gggggctta 
cgagcccac ggaggagga atctcaatg gccttcgga 
cgacgaaga cgaaacgct ggggaacca gctcaagaa 
caaggccaa gatcgagga ggagaaaac gccggagat 
tatacccaa aaaccccga aactaacga tgtgcccga 
ccccacggg aatacccaa cttctggtt gaccatctt 
ccagaacgt taaccttct atccgagat gattcagcc 
cccagacga acccatcct tagccatct gcaagacat 
caaagtcga ctgttccga aagcccaat gagcttcac 
tctatcatt ccacttctc ctccaaccc ctatttcac 
gaattcggt cctcacgaa agaatactt catgaagtg 
cgagccgga agaggacga tccgtttaa tttcgaggg 
cccggaaat ttacaagtg caagggttg caccattaa 
ctggaacaa gggtatgaa cgtgacgct gaaaactgt 
gaagaagaa gcagaagca taagagccg cggtgtggt 
ccgtactgt gaccaagac cgttcagaa cgactcgtt 
cttcaattt ctttgcgcc gcctgaact ccccgagaa 
cgccaaagg ggaggaaat cgacgacga gttgcgcca 
gttactcac tactgactt tgagattgg gcattacat 
tagggagag aattgttcc aagggccgt gttgttcta 
cacagggga agcgattga agatgagga tgaggatta 
cgaggatga cgatgatct ggaggacac cactgaaga 
aagcgattc cgataaaga ggaaaacga taaggcggc 
cagagacgc gaattgcaa acaacaata agtgggtta 
ttttcttcc tattctgcg cttataatt gtaacttat 
ttaatttct tttgtgact aaattgtta gatttggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY