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Gene information
General information
TC032798
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007471
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 4015155..4016744
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032798
    iBeetle screen iB_01199
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    elytra proximal blistered - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032798 RA
      MANTKPEQV DESLEDSSP EEEEAEQKT PNKFLDFTN 
      VLATLQSQR NTDKLGQLP PAVKRRIKA LKKLQLETT 
      NIEAKFFAE VHALECKYH KLYVPMYEK RNTIVTGAY 
      EPTEEESQW PSDDEDETL GNQLKNKAK IEEEKTPEI 
      IPKNPETND VPDPTGIPN FWLTIFQNV NLLSEMIQP 
      PDEPILSHL QDIKVDCSE SPMSFTLSF HFSSNPYFT 
      NSVLTKEYF MKCEPEEDD PFNFEGPEI YKCKGCTIN 
      WNKGMNVTL KTVKKKQKH KSRGVVRTV TKTVQNDSF 
      FNFFAPPEL PENAKGEEI DDELRQLLT TDFEIGHYI 
      RERIVPRAV LFYTGEAIE DEDEDYEDD DDLEDTTEE 
      SDSDKEEND KAARDANCK QQ
    CDS sequence
    • >TC032798 RA
      atggcaaat acaaaaccg gagcaagtg gacgagtcg 
      ctggaggac tcctcgccc gaggaggag gaggccgag 
      cagaagacc cccaacaag tttctcgac ttcaccaac 
      gtcttggcc acgctgcag agccagcgg aacaccgac 
      aagctgggg cagctgccc ccggccgtc aagcggcgg 
      atcaaagcc ctgaagaag ctccagctg gagacgacc 
      aacatcgag gccaagttc ttcgctgag gtgcacgcg 
      ctcgaatgt aaataccac aaattgtac gtgcccatg 
      tacgaaaag aggaacact atcgtgacg ggggcttac 
      gagcccacg gaggaggaa tctcaatgg ccttcggac 
      gacgaagac gaaacgctg gggaaccag ctcaagaac 
      aaggccaag atcgaggag gagaaaacg ccggagatt 
      atacccaaa aaccccgaa actaacgat gtgcccgac 
      cccacggga atacccaac ttctggttg accatcttc 
      cagaacgtt aaccttcta tccgagatg attcagccc 
      ccagacgaa cccatcctt agccatctg caagacatc 
      aaagtcgac tgttccgaa agcccaatg agcttcact 
      ctatcattc cacttctcc tccaacccc tatttcacg 
      aattcggtc ctcacgaaa gaatacttc atgaagtgc 
      gagccggaa gaggacgat ccgtttaat ttcgagggc 
      ccggaaatt tacaagtgc aagggttgc accattaac 
      tggaacaag ggtatgaac gtgacgctg aaaactgtg 
      aagaagaag cagaagcat aagagccgc ggtgtggtc 
      cgtactgtg accaagacc gttcagaac gactcgttc 
      ttcaatttc tttgcgccg cctgaactc cccgagaac 
      gccaaaggg gaggaaatc gacgacgag ttgcgccag 
      ttactcact actgacttt gagattggg cattacatt 
      agggagaga attgttcca agggccgtg ttgttctac 
      acaggggaa gcgattgaa gatgaggat gaggattac 
      gaggatgac gatgatctg gaggacacc actgaagaa 
      agcgattcc gataaagag gaaaacgat aaggcggcc 
      agagacgcg aattgcaaa caacaataa
    mRNA sequence
    • >TC032798 RA
      gccggtcca aattcaaac gctgcgcag ctgcaggaa 
      atcgtcttt gtagcgacg tagctggtg tcaaatttt 
      ctctttctc gtcgttgcc gttgcctgt gtcccccgt 
      cttcgtatt ccagcatca aacagccgg caaaagtaa 
      aatggcaaa tacaaaacc ggagcaagt ggacgagtc 
      gctggagga ctcctcgcc cgaggagga ggaggccga 
      gcagaagac ccccaacaa gtttctcga cttcaccaa 
      cgtcttggc cacgctgca gagccagcg gaacaccga 
      caagctggg gcagctgcc cccggccgt caagcggcg 
      gatcaaagc cctgaagaa gctccagct ggagacgac 
      caacatcga ggccaagtt cttcgctga ggtgcacgc 
      gctcgaatg taaatacca caaattgta cgtgcccat 
      gtacgaaaa gaggaacac tatcgtgac gggggctta 
      cgagcccac ggaggagga atctcaatg gccttcgga 
      cgacgaaga cgaaacgct ggggaacca gctcaagaa 
      caaggccaa gatcgagga ggagaaaac gccggagat 
      tatacccaa aaaccccga aactaacga tgtgcccga 
      ccccacggg aatacccaa cttctggtt gaccatctt 
      ccagaacgt taaccttct atccgagat gattcagcc 
      cccagacga acccatcct tagccatct gcaagacat 
      caaagtcga ctgttccga aagcccaat gagcttcac 
      tctatcatt ccacttctc ctccaaccc ctatttcac 
      gaattcggt cctcacgaa agaatactt catgaagtg 
      cgagccgga agaggacga tccgtttaa tttcgaggg 
      cccggaaat ttacaagtg caagggttg caccattaa 
      ctggaacaa gggtatgaa cgtgacgct gaaaactgt 
      gaagaagaa gcagaagca taagagccg cggtgtggt 
      ccgtactgt gaccaagac cgttcagaa cgactcgtt 
      cttcaattt ctttgcgcc gcctgaact ccccgagaa 
      cgccaaagg ggaggaaat cgacgacga gttgcgcca 
      gttactcac tactgactt tgagattgg gcattacat 
      tagggagag aattgttcc aagggccgt gttgttcta 
      cacagggga agcgattga agatgagga tgaggatta 
      cgaggatga cgatgatct ggaggacac cactgaaga 
      aagcgattc cgataaaga ggaaaacga taaggcggc 
      cagagacgc gaattgcaa acaacaata agtgggtta 
      ttttcttcc tattctgcg cttataatt gtaacttat 
      ttaatttct tttgtgact aaattgtta gatttggt