| General information | |||
| TC007470 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 4040766..4049514 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007470 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01198 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC007470 RA
MSCTKEEIV KCCASDARD AKYALNNND FAKHRFPKK QAVDVRFEN ITYTTWSWS MTRFQKETK RILHGVSGL FKAGEISVI MGPSGAGKT TLLNILAGF TTRGSAGVV KLNDTIRDK SPRFRKLSA YIPQDEELR MALTVKEAM TFAANLKLG YSVSNDYKK QQVLELLEM LGLENSHHT LTSRLSGGQ RKRLAVALE LLSNPPILY LDEPTTGLD SSSCTQCVS LLKRLAQEG RTIICTIHQ PSALLFEMF DKLYALSAG RCIYDGNTK DLVPHLSSL GLQCPPYHN PADFMMEVA IGEYGTDMN VLAEAAKVK QFDKIGEST KEKESMSCS LFDEIKPEE KAHDKSPLP AAVVMQFLL LYKRNLLTT KRGYIYFIN RLLAHVAIG LIFGYLYKD VGFGANTIL ANYVYLYGS LLLIVYTGK MSVTISFPL EMKILTREH FNRWYKLTP YLLSVILIE IPFQVICTW TYIAISYWL TAQPVDFRL YLFILFCTL STLCAQSWG YFIGATTPI KVAVFLGPV IACLFSVFG FCIRFFDTP YFFRWLFHV SYYRAGFQG IVYSIYGLN RPELYCPEE AIYCHYSDP TNFLKEMDI VDVDLVSNI SVIVVIWCL MNAATYLTL WIKLNKR
- >TC007470 RA
atgtcctgc actaaagag gagatcgtc aagtgctgt gccagcgac gcccgcgac gccaaatac gccctgaac aacaatgac ttcgccaaa caccgcttc ccgaaaaaa caggccgtc gacgtccga ttcgaaaac atcacatat accacatgg agttggtcc atgacccgc tttcaaaaa gaaacgaaa cgcatcctt catggcgtt tccggcctt ttcaaagcc ggagagatc tcggtgatt atgggacca tcgggcgct ggcaaaacg actctactt aatatctta gccggattc acgacgcgt ggcagcgcc ggagtcgtg aaactgaac gatacgatc cgtgacaag agcccgcgt ttccggaag ttgtccgcg tacatcccc caggacgag gaacttcgc atggctttg acagtcaag gaggcaatg accttcgcc gcaaatctg aaactagga tattccgtt tcgaatgat tacaaaaaa cagcaggtg ttggagctt ttggaaatg ctgggtttg gagaacagc caccacacg ctgacttcg cgcctctcc gggggccag cggaaacgc ttagcggtg gctttggag ctgttgagc aatccaccg attttgtat ctcgatgaa ccgacgacc ggtttggat agctcgtcg tgcacgcaa tgtgtttca cttttgaaa cggctggcg caggaagga aggaccatc atttgcacc atccaccag ccgtcggcg ctgcttttc gaaatgttc gacaagctg tacgccctg tcggcgggg cggtgcatc tacgacggc aacacgaag gacctggtg ccccatttg tccagtctg gggctccag tgccccccg taccacaac ccggccgat ttcatgatg gaagtcgcc attggagaa tacggaact gacatgaac gtcttagcc gaggcggcg aaagttaaa cagttcgat aagatcggc gaaagtacc aaagaaaaa gaatccatg tcgtgcagc ctatttgac gaaatcaag ccggaagag aaggctcac gacaagtcg ccactaccg gccgctgta gtaatgcag ttcttatta ttatacaaa cggaatttg ttgacgacg aaacggggt tatatttac ttcattaat cggttattg gcccacgtg gccattggg ttaattttc ggctatctc tacaaggac gttggtttt ggggccaac acaattcta gccaactac gtttatctc tacggatcg ttacttctg attgtgtac accggaaaa atgtcagtc acaatatca ttccccctt gaaatgaaa atcttgacg cgggagcac ttcaatcgg tggtacaaa ctgaccccc tacttactc tctgtgata ttaatcgaa atcccgttt caagtgatt tgcacttgg acgtacatc gcgatcagt tactggctg acggcacaa cccgtagat ttccgtttg tatttattc atcctgttt tgcaccctt tccacgctc tgcgcccag tcctggggc tacttcatc ggggccacg acccccatc aaagttgca gtatttctc ggcccagtg atcgcctgt cttttctcc gtgttcggc ttctgtatt cgatttttc gacactccg tactttttc cggtggttg ttccacgtg agctactac agggcgggc tttcaaggg attgtttac tcgatctat ggcctgaat cggcccgaa ttgtactgt cccgaggag gcgatttac tgtcactat tccgacccc acaaacttt ttgaaagag atggatatt gtcgatgtg gacctggtg tccaacatt tctgtgatt gtcgtgatc tggtgcttg atgaatgcg gcgacgtac ctcaccttg tggattaag ctgaataag cgctaa
- >TC007470 RA
acccagcac ttgcatcga taacaaact caaactcgc aaactgttc catttcatt agtagtgcc ttagaggtg cgatgatct actttttaa atgttttaa gaacggtgg gatgttatc ttgagtgaa agcatgtcc tgcactaaa gaggagatc gtcaagtgc tgtgccagc gacgcccgc gacgccaaa tacgccctg aacaacaat gacttcgcc aaacaccgc ttcccgaaa aaacaggcc gtcgacgtc cgattcgaa aacatcaca tataccaca tggagttgg tccatgacc cgctttcaa aaagaaacg aaacgcatc cttcatggc gtttccggc cttttcaaa gccggagag atctcggtg attatggga ccatcgggc gctggcaaa acgactcta cttaatatc ttagccgga ttcacgacg cgtggcagc gccggagtc gtgaaactg aacgatacg atccgtgac aagagcccg cgtttccgg aagttgtcc gcgtacatc ccccaggac gaggaactt cgcatggct ttgacagtc aaggaggca atgaccttc gccgcaaat ctgaaacta ggatattcc gtttcgaat gattacaaa aaacagcag gtgttggag cttttggaa atgctgggt ttggagaac agccaccac acgctgact tcgcgcctc tccgggggc cagcggaaa cgcttagcg gtggctttg gagctgttg agcaatcca ccgattttg tatctcgat gaaccgacg accggtttg gatagctcg tcgtgcacg caatgtgtt tcacttttg aaacggctg gcgcaggaa ggaaggacc atcatttgc accatccac cagccgtcg gcgctgctt ttcgaaatg ttcgacaag ctgtacgcc ctgtcggcg gggcggtgc atctacgac ggcaacacg aaggacctg gtgccccat ttgtccagt ctggggctc cagtgcccc ccgtaccac aacccggcc gatttcatg atggaagtc gccattgga gaatacgga actgacatg aacgtctta gccgaggcg gcgaaagtt aaacagttc gataagatc ggcgaaagt accaaagaa aaagaatcc atgtcgtgc agcctattt gacgaaatc aagccggaa gagaaggct cacgacaag tcgccacta ccggccgct gtagtaatg cagttctta ttattatac aaacggaat ttgttgacg acgaaacgg ggttatatt tacttcatt aatcggtta ttggcccac gtggccatt gggttaatt ttcggctat ctctacaag gacgttggt tttggggcc aacacaatt ctagccaac tacgtttat ctctacgga tcgttactt ctgattgtg tacaccgga aaaatgtca gtcacaata tcattcccc cttgaaatg aaaatcttg acgcgggag cacttcaat cggtggtac aaactgacc ccctactta ctctctgtg atattaatc gaaatcccg tttcaagtg atttgcact tggacgtac atcgcgatc agttactgg ctgacggca caacccgta gatttccgt ttgtattta ttcatcctg ttttgcacc ctttccacg ctctgcgcc cagtcctgg ggctacttc atcggggcc acgaccccc atcaaagtt gcagtattt ctcggccca gtgatcgcc tgtcttttc tccgtgttc ggcttctgt attcgattt ttcgacact ccgtacttt ttccggtgg ttgttccac gtgagctac tacagggcg ggctttcaa gggattgtt tactcgatc tatggcctg aatcggccc gaattgtac tgtcccgag gaggcgatt tactgtcac tattccgac cccacaaac tttttgaaa gagatggat attgtcgat gtggacctg gtgtccaac atttctgtg attgtcgtg atctggtgc ttgatgaat gcggcgacg tacctcacc ttgtggatt aagctgaat aagcgctaa ctaatgaaa tttttattt atttgaaga ccaaagact tgtaacgtg gagtaaatg tgttcgcca ccgacgttg gtattaatt aatttcaag tgtattatt ttacgttta gaacgtggt tttagttat ttaactcgt ttctgggtt aaaataagt ttctcaact agtcacttt agattgtag attggtgga atatttaaa aggaaactt gccattagg cttattttt ataatgtag agtcgatgt gatagcttt ttgtatttt aatcaaata aatgacatt ttttcca
