Status
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Gene information
General information
TC007470 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4040766..4049514
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007470
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01198

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007470 RA
      MSCTKEEIV KCCASDARD AKYALNNND FAKHRFPKK 
      QAVDVRFEN ITYTTWSWS MTRFQKETK RILHGVSGL 
      FKAGEISVI MGPSGAGKT TLLNILAGF TTRGSAGVV 
      KLNDTIRDK SPRFRKLSA YIPQDEELR MALTVKEAM 
      TFAANLKLG YSVSNDYKK QQVLELLEM LGLENSHHT 
      LTSRLSGGQ RKRLAVALE LLSNPPILY LDEPTTGLD 
      SSSCTQCVS LLKRLAQEG RTIICTIHQ PSALLFEMF 
      DKLYALSAG RCIYDGNTK DLVPHLSSL GLQCPPYHN 
      PADFMMEVA IGEYGTDMN VLAEAAKVK QFDKIGEST 
      KEKESMSCS LFDEIKPEE KAHDKSPLP AAVVMQFLL 
      LYKRNLLTT KRGYIYFIN RLLAHVAIG LIFGYLYKD 
      VGFGANTIL ANYVYLYGS LLLIVYTGK MSVTISFPL 
      EMKILTREH FNRWYKLTP YLLSVILIE IPFQVICTW 
      TYIAISYWL TAQPVDFRL YLFILFCTL STLCAQSWG 
      YFIGATTPI KVAVFLGPV IACLFSVFG FCIRFFDTP 
      YFFRWLFHV SYYRAGFQG IVYSIYGLN RPELYCPEE 
      AIYCHYSDP TNFLKEMDI VDVDLVSNI SVIVVIWCL 
      MNAATYLTL WIKLNKR
    CDS sequence
    • >TC007470 RA
      atgtcctgc actaaagag gagatcgtc aagtgctgt 
      gccagcgac gcccgcgac gccaaatac gccctgaac 
      aacaatgac ttcgccaaa caccgcttc ccgaaaaaa 
      caggccgtc gacgtccga ttcgaaaac atcacatat 
      accacatgg agttggtcc atgacccgc tttcaaaaa 
      gaaacgaaa cgcatcctt catggcgtt tccggcctt 
      ttcaaagcc ggagagatc tcggtgatt atgggacca 
      tcgggcgct ggcaaaacg actctactt aatatctta 
      gccggattc acgacgcgt ggcagcgcc ggagtcgtg 
      aaactgaac gatacgatc cgtgacaag agcccgcgt 
      ttccggaag ttgtccgcg tacatcccc caggacgag 
      gaacttcgc atggctttg acagtcaag gaggcaatg 
      accttcgcc gcaaatctg aaactagga tattccgtt 
      tcgaatgat tacaaaaaa cagcaggtg ttggagctt 
      ttggaaatg ctgggtttg gagaacagc caccacacg 
      ctgacttcg cgcctctcc gggggccag cggaaacgc 
      ttagcggtg gctttggag ctgttgagc aatccaccg 
      attttgtat ctcgatgaa ccgacgacc ggtttggat 
      agctcgtcg tgcacgcaa tgtgtttca cttttgaaa 
      cggctggcg caggaagga aggaccatc atttgcacc 
      atccaccag ccgtcggcg ctgcttttc gaaatgttc 
      gacaagctg tacgccctg tcggcgggg cggtgcatc 
      tacgacggc aacacgaag gacctggtg ccccatttg 
      tccagtctg gggctccag tgccccccg taccacaac 
      ccggccgat ttcatgatg gaagtcgcc attggagaa 
      tacggaact gacatgaac gtcttagcc gaggcggcg 
      aaagttaaa cagttcgat aagatcggc gaaagtacc 
      aaagaaaaa gaatccatg tcgtgcagc ctatttgac 
      gaaatcaag ccggaagag aaggctcac gacaagtcg 
      ccactaccg gccgctgta gtaatgcag ttcttatta 
      ttatacaaa cggaatttg ttgacgacg aaacggggt 
      tatatttac ttcattaat cggttattg gcccacgtg 
      gccattggg ttaattttc ggctatctc tacaaggac 
      gttggtttt ggggccaac acaattcta gccaactac 
      gtttatctc tacggatcg ttacttctg attgtgtac 
      accggaaaa atgtcagtc acaatatca ttccccctt 
      gaaatgaaa atcttgacg cgggagcac ttcaatcgg 
      tggtacaaa ctgaccccc tacttactc tctgtgata 
      ttaatcgaa atcccgttt caagtgatt tgcacttgg 
      acgtacatc gcgatcagt tactggctg acggcacaa 
      cccgtagat ttccgtttg tatttattc atcctgttt 
      tgcaccctt tccacgctc tgcgcccag tcctggggc 
      tacttcatc ggggccacg acccccatc aaagttgca 
      gtatttctc ggcccagtg atcgcctgt cttttctcc 
      gtgttcggc ttctgtatt cgatttttc gacactccg 
      tactttttc cggtggttg ttccacgtg agctactac 
      agggcgggc tttcaaggg attgtttac tcgatctat 
      ggcctgaat cggcccgaa ttgtactgt cccgaggag 
      gcgatttac tgtcactat tccgacccc acaaacttt 
      ttgaaagag atggatatt gtcgatgtg gacctggtg 
      tccaacatt tctgtgatt gtcgtgatc tggtgcttg 
      atgaatgcg gcgacgtac ctcaccttg tggattaag 
      ctgaataag cgctaa
    mRNA sequence
    • >TC007470 RA
      acccagcac ttgcatcga taacaaact caaactcgc 
      aaactgttc catttcatt agtagtgcc ttagaggtg 
      cgatgatct actttttaa atgttttaa gaacggtgg 
      gatgttatc ttgagtgaa agcatgtcc tgcactaaa 
      gaggagatc gtcaagtgc tgtgccagc gacgcccgc 
      gacgccaaa tacgccctg aacaacaat gacttcgcc 
      aaacaccgc ttcccgaaa aaacaggcc gtcgacgtc 
      cgattcgaa aacatcaca tataccaca tggagttgg 
      tccatgacc cgctttcaa aaagaaacg aaacgcatc 
      cttcatggc gtttccggc cttttcaaa gccggagag 
      atctcggtg attatggga ccatcgggc gctggcaaa 
      acgactcta cttaatatc ttagccgga ttcacgacg 
      cgtggcagc gccggagtc gtgaaactg aacgatacg 
      atccgtgac aagagcccg cgtttccgg aagttgtcc 
      gcgtacatc ccccaggac gaggaactt cgcatggct 
      ttgacagtc aaggaggca atgaccttc gccgcaaat 
      ctgaaacta ggatattcc gtttcgaat gattacaaa 
      aaacagcag gtgttggag cttttggaa atgctgggt 
      ttggagaac agccaccac acgctgact tcgcgcctc 
      tccgggggc cagcggaaa cgcttagcg gtggctttg 
      gagctgttg agcaatcca ccgattttg tatctcgat 
      gaaccgacg accggtttg gatagctcg tcgtgcacg 
      caatgtgtt tcacttttg aaacggctg gcgcaggaa 
      ggaaggacc atcatttgc accatccac cagccgtcg 
      gcgctgctt ttcgaaatg ttcgacaag ctgtacgcc 
      ctgtcggcg gggcggtgc atctacgac ggcaacacg 
      aaggacctg gtgccccat ttgtccagt ctggggctc 
      cagtgcccc ccgtaccac aacccggcc gatttcatg 
      atggaagtc gccattgga gaatacgga actgacatg 
      aacgtctta gccgaggcg gcgaaagtt aaacagttc 
      gataagatc ggcgaaagt accaaagaa aaagaatcc 
      atgtcgtgc agcctattt gacgaaatc aagccggaa 
      gagaaggct cacgacaag tcgccacta ccggccgct 
      gtagtaatg cagttctta ttattatac aaacggaat 
      ttgttgacg acgaaacgg ggttatatt tacttcatt 
      aatcggtta ttggcccac gtggccatt gggttaatt 
      ttcggctat ctctacaag gacgttggt tttggggcc 
      aacacaatt ctagccaac tacgtttat ctctacgga 
      tcgttactt ctgattgtg tacaccgga aaaatgtca 
      gtcacaata tcattcccc cttgaaatg aaaatcttg 
      acgcgggag cacttcaat cggtggtac aaactgacc 
      ccctactta ctctctgtg atattaatc gaaatcccg 
      tttcaagtg atttgcact tggacgtac atcgcgatc 
      agttactgg ctgacggca caacccgta gatttccgt 
      ttgtattta ttcatcctg ttttgcacc ctttccacg 
      ctctgcgcc cagtcctgg ggctacttc atcggggcc 
      acgaccccc atcaaagtt gcagtattt ctcggccca 
      gtgatcgcc tgtcttttc tccgtgttc ggcttctgt 
      attcgattt ttcgacact ccgtacttt ttccggtgg 
      ttgttccac gtgagctac tacagggcg ggctttcaa 
      gggattgtt tactcgatc tatggcctg aatcggccc 
      gaattgtac tgtcccgag gaggcgatt tactgtcac 
      tattccgac cccacaaac tttttgaaa gagatggat 
      attgtcgat gtggacctg gtgtccaac atttctgtg 
      attgtcgtg atctggtgc ttgatgaat gcggcgacg 
      tacctcacc ttgtggatt aagctgaat aagcgctaa 
      ctaatgaaa tttttattt atttgaaga ccaaagact 
      tgtaacgtg gagtaaatg tgttcgcca ccgacgttg 
      gtattaatt aatttcaag tgtattatt ttacgttta 
      gaacgtggt tttagttat ttaactcgt ttctgggtt 
      aaaataagt ttctcaact agtcacttt agattgtag 
      attggtgga atatttaaa aggaaactt gccattagg 
      cttattttt ataatgtag agtcgatgt gatagcttt 
      ttgtatttt aatcaaata aatgacatt ttttcca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY