Status
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Gene information
General information
TC007467 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4074968..4079671
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007467
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01197

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 28.57% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    legs tarsus partially colour darker - Number of animals showing the phenotype: 7


    legs tarsus partially colour lighter - Number of animals showing the phenotype: 7

    legs tarsus potentially necrotic - Number of animals showing the phenotype: 7

    remarks: later than schedule, hours 96
    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007467 RA
      MDENLEEIR KNNVLNLPQ RPAVEIEFQ DLTYTVPQG 
RKGSKLILR SISGKFRSG QLTAILGPS GSGKSTLLN 
ILAGYKCRE ATGTILING EPRNLKEFR EIARYIMQE 
DLIQPLLSV DEAMMIAAN LKLGKHISV EDKSKIILD 
ILELLRLSN TRNTHTNKL SGGERKRLS IALELLNNP 
PVLFLDEPT TGLDDLSCS QCVSLLKQL AAGGRTVIC 
SIHTPSAKL FSMFDKVYV ISSGQCTYQ GYGPEAVPY 
LSTLGITCP THYNPADFI MEVCSGEYG DCQDKMVSA 
IDNGRNEFI KDSVVPLFE STSSSQKME IFEIVSSKE 
GKNSSWDQF KILLMRMWV QMWRNKSYL LLKVVLHII 
LGLLIGNLY IGIGEDGSK TIFNFGFFF TCVIFFMYI 
PMMPVLLSF PLEIQYLKR EHFNQWYSI GSYFCALTV 
STLPVQMIL GSLYLSMVY IFSYQPLEI ERIVPFFAI 
CFLTSIISE SFGLLISST LNLLNGMFV GPVMMVPFI 
VFSAYGFGE GYASIPILI KIMMRFSYL RYSFEALVL 
VMLRGRKLQ CPEEEEFCI FTDLDEFIE IMAMNNSVI 
WVDIVALII FLVLVRSAS FYLLRQRLT PNKTFMALQ 
YIGRFIKTR MSEGR
    CDS sequence
    • >TC007467 RA
      atggatgaa aatcttgaa gagatccgt aaaaacaac 
gtcctgaat ctgccacaa aggccggcg gtggagatc 
gagtttcaa gacctgacc tacactgtg ccacaaggc 
aggaaaggt tctaagcta atcctacgc agcattagt 
gggaaattt cgctcgggc caattgacc gcaattttg 
gggccttcc gggtcgggg aaaagtact cttctgaat 
attctagcc ggttacaag tgtcgagaa gccaccggc 
actatttta atcaacgga gagccgaga aaccttaaa 
gaatttagg gagatcgct cgatatatt atgcaagaa 
gatcttata caaccgctg ctgagcgtg gacgaggct 
atgatgatc gcggcgaac ctcaagctc ggcaaacac 
atttcagtg gaagacaaa tcgaaaatt attttagat 
attttagaa ttgttacgg ttgagtaac acgagaaat 
acacataca aacaaactg tcaggaggg gaacggaaa 
cggctatct atagctctg gaactgttg aacaaccca 
cctgtactt ttcttggac gaaccgaca accggtttg 
gacgacttg tcctgctcc caatgcgtc tccctcctc 
aagcaactg gccgccggg ggccgaact gtgatatgc 
tcgatccac acaccaagt gccaaactc ttctcaatg 
ttcgacaaa gtttacgta atttcgtcg ggacaatgc 
acgtatcaa ggctacggc ccggaggcg gtcccatat 
ttatcaaca ctcggtatt acttgcccc acacattac 
aacccagcg gatttcatc atggaggtg tgttcgggg 
gaatatggc gactgtcag gacaaaatg gtgtcggca 
atcgacaat ggccgcaat gaatttatc aaagacagt 
gtggtaccg ctgtttgag agcacctca agtagccag 
aaaatggaa atatttgaa attgtgtcc agtaaggag 
gggaagaac tcgagttgg gaccaattt aagatattg 
ctgatgcga atgtgggtg cagatgtgg cggaacaag 
agctatttg ttgctaaaa gtggtgcta catatcatt 
ttggggctc ctgattggg aacttgtac atcggaatt 
ggggaagat gggtccaag acgattttt aatttcggg 
tttttcttc acctgtgtt atcttcttt atgtatatt 
cccatgatg ccagtcttg ttatcgttc cctctagag 
atacagtac ctaaaacgg gaacacttc aaccaatgg 
tacagcata ggttcttat ttttgtgcc ttaacagta 
tcaacgcta ccagtacag atgattctg ggtagtttg 
tacctcagt atggtatat attttctcg taccaacct 
ctggaaatc gaacgaata gtaccattc ttcgccatt 
tgtttttta acaagtatc atatccgaa agcttcggg 
ttgttaatc tcatcgact ctcaactta cttaatgga 
atgtttgtc gggcccgtc atgatggta ccatttata 
gtattctcc gcttacggt tttggcgaa ggctacgcc 
agtattccc atccttatc aaaataatg atgcggttc 
agttatctg aggtactcg ttcgaggcc ctagtctta 
gtcatgttg aggggaaga aagttgcaa tgtcccgaa 
gaagaggaa ttttgcata tttaccgac ttggatgaa 
tttatcgaa ataatggca atgaataat tcggttatt 
tgggttgat attgtcgca cttatcata tttttggtg 
cttgttaga agtgccagc ttttattta ttgaggcag 
aggctcaca cctaacaag actttcatg gccttacag 
tatatcggt aggtttatt aagacgcga atgagcgaa 
ggtcgttaa
    mRNA sequence
    • >TC007467 RA
      tgtagctga gccacaaag aggggtttc aatgttgca 
tttccaagc gagtccatc ctccttagc cagtcgatc 
gctgagtgt ttttaagtt cgcattgat tatctcatg 
agtgtaact ttcgccgtt tataaccgg ttagatttt 
aaatattaa aaacgtaaa tatatgtag agtgaaagt 
aaaaaatgg atgaaaatc ttgaagaga tccgtaaaa 
acaacgtcc tgaatctgc cacaaaggc cggcggtgg 
agatcgagt ttcaagacc tgacctaca ctgtgccac 
aaggcagga aaggttcta agctaatcc tacgcagca 
ttagtggga aatttcgct cgggccaat tgaccgcaa 
ttttggggc cttccgggt cggggaaaa gtactcttc 
tgaatattc tagccggtt acaagtgtc gagaagcca 
ccggcacta ttttaatca acggagagc cgagaaacc 
ttaaagaat ttagggaga tcgctcgat atattatgc 
aagaagatc ttatacaac cgctgctga gcgtggacg 
aggctatga tgatcgcgg cgaacctca agctcggca 
aacacattt cagtggaag acaaatcga aaattattt 
tagatattt tagaattgt tacggttga gtaacacga 
gaaatacac atacaaaca aactgtcag gaggggaac 
ggaaacggc tatctatag ctctggaac tgttgaaca 
acccacctg tacttttct tggacgaac cgacaaccg 
gtttggacg acttgtcct gctcccaat gcgtctccc 
tcctcaagc aactggccg ccgggggcc gaactgtga 
tatgctcga tccacacac caagtgcca aactcttct 
caatgttcg acaaagttt acgtaattt cgtcgggac 
aatgcacgt atcaaggct acggcccgg aggcggtcc 
catatttat caacactcg gtattactt gccccacac 
attacaacc cagcggatt tcatcatgg aggtgtgtt 
cgggggaat atggcgact gtcaggaca aaatggtgt 
cggcaatcg acaatggcc gcaatgaat ttatcaaag 
acagtgtgg taccgctgt ttgagagca cctcaagta 
gccagaaaa tggaaatat ttgaaattg tgtccagta 
aggagggga agaactcga gttgggacc aatttaaga 
tattgctga tgcgaatgt gggtgcaga tgtggcgga 
acaagagct atttgttgc taaaagtgg tgctacata 
tcattttgg ggctcctga ttgggaact tgtacatcg 
gaattgggg aagatgggt ccaagacga tttttaatt 
tcgggtttt tcttcacct gtgttatct tctttatgt 
atattccca tgatgccag tcttgttat cgttccctc 
tagagatac agtacctaa aacgggaac acttcaacc 
aatggtaca gcataggtt cttattttt gtgccttaa 
cagtatcaa cgctaccag tacagatga ttctgggta 
gtttgtacc tcagtatgg tatatattt tctcgtacc 
aacctctgg aaatcgaac gaatagtac cattcttcg 
ccatttgtt ttttaacaa gtatcatat ccgaaagct 
tcgggttgt taatctcat cgactctca acttactta 
atggaatgt ttgtcgggc ccgtcatga tggtaccat 
ttatagtat tctccgctt acggttttg gcgaaggct 
acgccagta ttcccatcc ttatcaaaa taatgatgc 
ggttcagtt atctgaggt actcgttcg aggccctag 
tcttagtca tgttgaggg gaagaaagt tgcaatgtc 
ccgaagaag aggaatttt gcatattta ccgacttgg 
atgaattta tcgaaataa tggcaatga ataattcgg 
ttatttggg ttgatattg tcgcactta tcatatttt 
tggtgcttg ttagaagtg ccagctttt atttattga 
ggcagaggc tcacaccta acaagactt tcatggcct 
tacagtata tcggtaggt ttattaaga cgcgaatga 
gcgaaggtc gttaatgac agtgatata ggttagtgt 
ggattttgt attgttaat taaatagag ctttatttt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY