General information | |||
TC007467 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 4074968..4079671 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC007467 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01197 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 28.57% (includes death as larva, prepupa, pupa, adult)
legs tarsus partially colour lighter - Number of animals showing the phenotype: 7
legs tarsus potentially necrotic - Number of animals showing the phenotype: 7
remarks: later than schedule, hours 96
ovary without detectable defects - Number of animals showing the phenotype: 4
- >TC007467 RA
MDENLEEIR KNNVLNLPQ RPAVEIEFQ DLTYTVPQG RKGSKLILR SISGKFRSG QLTAILGPS GSGKSTLLN ILAGYKCRE ATGTILING EPRNLKEFR EIARYIMQE DLIQPLLSV DEAMMIAAN LKLGKHISV EDKSKIILD ILELLRLSN TRNTHTNKL SGGERKRLS IALELLNNP PVLFLDEPT TGLDDLSCS QCVSLLKQL AAGGRTVIC SIHTPSAKL FSMFDKVYV ISSGQCTYQ GYGPEAVPY LSTLGITCP THYNPADFI MEVCSGEYG DCQDKMVSA IDNGRNEFI KDSVVPLFE STSSSQKME IFEIVSSKE GKNSSWDQF KILLMRMWV QMWRNKSYL LLKVVLHII LGLLIGNLY IGIGEDGSK TIFNFGFFF TCVIFFMYI PMMPVLLSF PLEIQYLKR EHFNQWYSI GSYFCALTV STLPVQMIL GSLYLSMVY IFSYQPLEI ERIVPFFAI CFLTSIISE SFGLLISST LNLLNGMFV GPVMMVPFI VFSAYGFGE GYASIPILI KIMMRFSYL RYSFEALVL VMLRGRKLQ CPEEEEFCI FTDLDEFIE IMAMNNSVI WVDIVALII FLVLVRSAS FYLLRQRLT PNKTFMALQ YIGRFIKTR MSEGR
- >TC007467 RA
atggatgaa aatcttgaa gagatccgt aaaaacaac gtcctgaat ctgccacaa aggccggcg gtggagatc gagtttcaa gacctgacc tacactgtg ccacaaggc aggaaaggt tctaagcta atcctacgc agcattagt gggaaattt cgctcgggc caattgacc gcaattttg gggccttcc gggtcgggg aaaagtact cttctgaat attctagcc ggttacaag tgtcgagaa gccaccggc actatttta atcaacgga gagccgaga aaccttaaa gaatttagg gagatcgct cgatatatt atgcaagaa gatcttata caaccgctg ctgagcgtg gacgaggct atgatgatc gcggcgaac ctcaagctc ggcaaacac atttcagtg gaagacaaa tcgaaaatt attttagat attttagaa ttgttacgg ttgagtaac acgagaaat acacataca aacaaactg tcaggaggg gaacggaaa cggctatct atagctctg gaactgttg aacaaccca cctgtactt ttcttggac gaaccgaca accggtttg gacgacttg tcctgctcc caatgcgtc tccctcctc aagcaactg gccgccggg ggccgaact gtgatatgc tcgatccac acaccaagt gccaaactc ttctcaatg ttcgacaaa gtttacgta atttcgtcg ggacaatgc acgtatcaa ggctacggc ccggaggcg gtcccatat ttatcaaca ctcggtatt acttgcccc acacattac aacccagcg gatttcatc atggaggtg tgttcgggg gaatatggc gactgtcag gacaaaatg gtgtcggca atcgacaat ggccgcaat gaatttatc aaagacagt gtggtaccg ctgtttgag agcacctca agtagccag aaaatggaa atatttgaa attgtgtcc agtaaggag gggaagaac tcgagttgg gaccaattt aagatattg ctgatgcga atgtgggtg cagatgtgg cggaacaag agctatttg ttgctaaaa gtggtgcta catatcatt ttggggctc ctgattggg aacttgtac atcggaatt ggggaagat gggtccaag acgattttt aatttcggg tttttcttc acctgtgtt atcttcttt atgtatatt cccatgatg ccagtcttg ttatcgttc cctctagag atacagtac ctaaaacgg gaacacttc aaccaatgg tacagcata ggttcttat ttttgtgcc ttaacagta tcaacgcta ccagtacag atgattctg ggtagtttg tacctcagt atggtatat attttctcg taccaacct ctggaaatc gaacgaata gtaccattc ttcgccatt tgtttttta acaagtatc atatccgaa agcttcggg ttgttaatc tcatcgact ctcaactta cttaatgga atgtttgtc gggcccgtc atgatggta ccatttata gtattctcc gcttacggt tttggcgaa ggctacgcc agtattccc atccttatc aaaataatg atgcggttc agttatctg aggtactcg ttcgaggcc ctagtctta gtcatgttg aggggaaga aagttgcaa tgtcccgaa gaagaggaa ttttgcata tttaccgac ttggatgaa tttatcgaa ataatggca atgaataat tcggttatt tgggttgat attgtcgca cttatcata tttttggtg cttgttaga agtgccagc ttttattta ttgaggcag aggctcaca cctaacaag actttcatg gccttacag tatatcggt aggtttatt aagacgcga atgagcgaa ggtcgttaa
- >TC007467 RA
tgtagctga gccacaaag aggggtttc aatgttgca tttccaagc gagtccatc ctccttagc cagtcgatc gctgagtgt ttttaagtt cgcattgat tatctcatg agtgtaact ttcgccgtt tataaccgg ttagatttt aaatattaa aaacgtaaa tatatgtag agtgaaagt aaaaaatgg atgaaaatc ttgaagaga tccgtaaaa acaacgtcc tgaatctgc cacaaaggc cggcggtgg agatcgagt ttcaagacc tgacctaca ctgtgccac aaggcagga aaggttcta agctaatcc tacgcagca ttagtggga aatttcgct cgggccaat tgaccgcaa ttttggggc cttccgggt cggggaaaa gtactcttc tgaatattc tagccggtt acaagtgtc gagaagcca ccggcacta ttttaatca acggagagc cgagaaacc ttaaagaat ttagggaga tcgctcgat atattatgc aagaagatc ttatacaac cgctgctga gcgtggacg aggctatga tgatcgcgg cgaacctca agctcggca aacacattt cagtggaag acaaatcga aaattattt tagatattt tagaattgt tacggttga gtaacacga gaaatacac atacaaaca aactgtcag gaggggaac ggaaacggc tatctatag ctctggaac tgttgaaca acccacctg tacttttct tggacgaac cgacaaccg gtttggacg acttgtcct gctcccaat gcgtctccc tcctcaagc aactggccg ccgggggcc gaactgtga tatgctcga tccacacac caagtgcca aactcttct caatgttcg acaaagttt acgtaattt cgtcgggac aatgcacgt atcaaggct acggcccgg aggcggtcc catatttat caacactcg gtattactt gccccacac attacaacc cagcggatt tcatcatgg aggtgtgtt cgggggaat atggcgact gtcaggaca aaatggtgt cggcaatcg acaatggcc gcaatgaat ttatcaaag acagtgtgg taccgctgt ttgagagca cctcaagta gccagaaaa tggaaatat ttgaaattg tgtccagta aggagggga agaactcga gttgggacc aatttaaga tattgctga tgcgaatgt gggtgcaga tgtggcgga acaagagct atttgttgc taaaagtgg tgctacata tcattttgg ggctcctga ttgggaact tgtacatcg gaattgggg aagatgggt ccaagacga tttttaatt tcgggtttt tcttcacct gtgttatct tctttatgt atattccca tgatgccag tcttgttat cgttccctc tagagatac agtacctaa aacgggaac acttcaacc aatggtaca gcataggtt cttattttt gtgccttaa cagtatcaa cgctaccag tacagatga ttctgggta gtttgtacc tcagtatgg tatatattt tctcgtacc aacctctgg aaatcgaac gaatagtac cattcttcg ccatttgtt ttttaacaa gtatcatat ccgaaagct tcgggttgt taatctcat cgactctca acttactta atggaatgt ttgtcgggc ccgtcatga tggtaccat ttatagtat tctccgctt acggttttg gcgaaggct acgccagta ttcccatcc ttatcaaaa taatgatgc ggttcagtt atctgaggt actcgttcg aggccctag tcttagtca tgttgaggg gaagaaagt tgcaatgtc ccgaagaag aggaatttt gcatattta ccgacttgg atgaattta tcgaaataa tggcaatga ataattcgg ttatttggg ttgatattg tcgcactta tcatatttt tggtgcttg ttagaagtg ccagctttt atttattga ggcagaggc tcacaccta acaagactt tcatggcct tacagtata tcggtaggt ttattaaga cgcgaatga gcgaaggtc gttaatgac agtgatata ggttagtgt ggattttgt attgttaat taaatagag ctttatttt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
