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Gene information
General information
TC007467 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 4074968..4079671
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007467
    iBeetle screen iB_01197
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 28.57% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    legs tarsus partially colour darker - Number of animals showing the phenotype: 7


    legs tarsus partially colour lighter - Number of animals showing the phenotype: 7

    legs tarsus potentially necrotic - Number of animals showing the phenotype: 7

    remarks: later than schedule, hours 96
    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007467 RA
      MDENLEEIR KNNVLNLPQ RPAVEIEFQ DLTYTVPQG 
      RKGSKLILR SISGKFRSG QLTAILGPS GSGKSTLLN 
      ILAGYKCRE ATGTILING EPRNLKEFR EIARYIMQE 
      DLIQPLLSV DEAMMIAAN LKLGKHISV EDKSKIILD 
      ILELLRLSN TRNTHTNKL SGGERKRLS IALELLNNP 
      PVLFLDEPT TGLDDLSCS QCVSLLKQL AAGGRTVIC 
      SIHTPSAKL FSMFDKVYV ISSGQCTYQ GYGPEAVPY 
      LSTLGITCP THYNPADFI MEVCSGEYG DCQDKMVSA 
      IDNGRNEFI KDSVVPLFE STSSSQKME IFEIVSSKE 
      GKNSSWDQF KILLMRMWV QMWRNKSYL LLKVVLHII 
      LGLLIGNLY IGIGEDGSK TIFNFGFFF TCVIFFMYI 
      PMMPVLLSF PLEIQYLKR EHFNQWYSI GSYFCALTV 
      STLPVQMIL GSLYLSMVY IFSYQPLEI ERIVPFFAI 
      CFLTSIISE SFGLLISST LNLLNGMFV GPVMMVPFI 
      VFSAYGFGE GYASIPILI KIMMRFSYL RYSFEALVL 
      VMLRGRKLQ CPEEEEFCI FTDLDEFIE IMAMNNSVI 
      WVDIVALII FLVLVRSAS FYLLRQRLT PNKTFMALQ 
      YIGRFIKTR MSEGR
    CDS sequence
    • >TC007467 RA
      atggatgaa aatcttgaa gagatccgt aaaaacaac 
      gtcctgaat ctgccacaa aggccggcg gtggagatc 
      gagtttcaa gacctgacc tacactgtg ccacaaggc 
      aggaaaggt tctaagcta atcctacgc agcattagt 
      gggaaattt cgctcgggc caattgacc gcaattttg 
      gggccttcc gggtcgggg aaaagtact cttctgaat 
      attctagcc ggttacaag tgtcgagaa gccaccggc 
      actatttta atcaacgga gagccgaga aaccttaaa 
      gaatttagg gagatcgct cgatatatt atgcaagaa 
      gatcttata caaccgctg ctgagcgtg gacgaggct 
      atgatgatc gcggcgaac ctcaagctc ggcaaacac 
      atttcagtg gaagacaaa tcgaaaatt attttagat 
      attttagaa ttgttacgg ttgagtaac acgagaaat 
      acacataca aacaaactg tcaggaggg gaacggaaa 
      cggctatct atagctctg gaactgttg aacaaccca 
      cctgtactt ttcttggac gaaccgaca accggtttg 
      gacgacttg tcctgctcc caatgcgtc tccctcctc 
      aagcaactg gccgccggg ggccgaact gtgatatgc 
      tcgatccac acaccaagt gccaaactc ttctcaatg 
      ttcgacaaa gtttacgta atttcgtcg ggacaatgc 
      acgtatcaa ggctacggc ccggaggcg gtcccatat 
      ttatcaaca ctcggtatt acttgcccc acacattac 
      aacccagcg gatttcatc atggaggtg tgttcgggg 
      gaatatggc gactgtcag gacaaaatg gtgtcggca 
      atcgacaat ggccgcaat gaatttatc aaagacagt 
      gtggtaccg ctgtttgag agcacctca agtagccag 
      aaaatggaa atatttgaa attgtgtcc agtaaggag 
      gggaagaac tcgagttgg gaccaattt aagatattg 
      ctgatgcga atgtgggtg cagatgtgg cggaacaag 
      agctatttg ttgctaaaa gtggtgcta catatcatt 
      ttggggctc ctgattggg aacttgtac atcggaatt 
      ggggaagat gggtccaag acgattttt aatttcggg 
      tttttcttc acctgtgtt atcttcttt atgtatatt 
      cccatgatg ccagtcttg ttatcgttc cctctagag 
      atacagtac ctaaaacgg gaacacttc aaccaatgg 
      tacagcata ggttcttat ttttgtgcc ttaacagta 
      tcaacgcta ccagtacag atgattctg ggtagtttg 
      tacctcagt atggtatat attttctcg taccaacct 
      ctggaaatc gaacgaata gtaccattc ttcgccatt 
      tgtttttta acaagtatc atatccgaa agcttcggg 
      ttgttaatc tcatcgact ctcaactta cttaatgga 
      atgtttgtc gggcccgtc atgatggta ccatttata 
      gtattctcc gcttacggt tttggcgaa ggctacgcc 
      agtattccc atccttatc aaaataatg atgcggttc 
      agttatctg aggtactcg ttcgaggcc ctagtctta 
      gtcatgttg aggggaaga aagttgcaa tgtcccgaa 
      gaagaggaa ttttgcata tttaccgac ttggatgaa 
      tttatcgaa ataatggca atgaataat tcggttatt 
      tgggttgat attgtcgca cttatcata tttttggtg 
      cttgttaga agtgccagc ttttattta ttgaggcag 
      aggctcaca cctaacaag actttcatg gccttacag 
      tatatcggt aggtttatt aagacgcga atgagcgaa 
      ggtcgttaa
    mRNA sequence
    • >TC007467 RA
      tgtagctga gccacaaag aggggtttc aatgttgca 
      tttccaagc gagtccatc ctccttagc cagtcgatc 
      gctgagtgt ttttaagtt cgcattgat tatctcatg 
      agtgtaact ttcgccgtt tataaccgg ttagatttt 
      aaatattaa aaacgtaaa tatatgtag agtgaaagt 
      aaaaaatgg atgaaaatc ttgaagaga tccgtaaaa 
      acaacgtcc tgaatctgc cacaaaggc cggcggtgg 
      agatcgagt ttcaagacc tgacctaca ctgtgccac 
      aaggcagga aaggttcta agctaatcc tacgcagca 
      ttagtggga aatttcgct cgggccaat tgaccgcaa 
      ttttggggc cttccgggt cggggaaaa gtactcttc 
      tgaatattc tagccggtt acaagtgtc gagaagcca 
      ccggcacta ttttaatca acggagagc cgagaaacc 
      ttaaagaat ttagggaga tcgctcgat atattatgc 
      aagaagatc ttatacaac cgctgctga gcgtggacg 
      aggctatga tgatcgcgg cgaacctca agctcggca 
      aacacattt cagtggaag acaaatcga aaattattt 
      tagatattt tagaattgt tacggttga gtaacacga 
      gaaatacac atacaaaca aactgtcag gaggggaac 
      ggaaacggc tatctatag ctctggaac tgttgaaca 
      acccacctg tacttttct tggacgaac cgacaaccg 
      gtttggacg acttgtcct gctcccaat gcgtctccc 
      tcctcaagc aactggccg ccgggggcc gaactgtga 
      tatgctcga tccacacac caagtgcca aactcttct 
      caatgttcg acaaagttt acgtaattt cgtcgggac 
      aatgcacgt atcaaggct acggcccgg aggcggtcc 
      catatttat caacactcg gtattactt gccccacac 
      attacaacc cagcggatt tcatcatgg aggtgtgtt 
      cgggggaat atggcgact gtcaggaca aaatggtgt 
      cggcaatcg acaatggcc gcaatgaat ttatcaaag 
      acagtgtgg taccgctgt ttgagagca cctcaagta 
      gccagaaaa tggaaatat ttgaaattg tgtccagta 
      aggagggga agaactcga gttgggacc aatttaaga 
      tattgctga tgcgaatgt gggtgcaga tgtggcgga 
      acaagagct atttgttgc taaaagtgg tgctacata 
      tcattttgg ggctcctga ttgggaact tgtacatcg 
      gaattgggg aagatgggt ccaagacga tttttaatt 
      tcgggtttt tcttcacct gtgttatct tctttatgt 
      atattccca tgatgccag tcttgttat cgttccctc 
      tagagatac agtacctaa aacgggaac acttcaacc 
      aatggtaca gcataggtt cttattttt gtgccttaa 
      cagtatcaa cgctaccag tacagatga ttctgggta 
      gtttgtacc tcagtatgg tatatattt tctcgtacc 
      aacctctgg aaatcgaac gaatagtac cattcttcg 
      ccatttgtt ttttaacaa gtatcatat ccgaaagct 
      tcgggttgt taatctcat cgactctca acttactta 
      atggaatgt ttgtcgggc ccgtcatga tggtaccat 
      ttatagtat tctccgctt acggttttg gcgaaggct 
      acgccagta ttcccatcc ttatcaaaa taatgatgc 
      ggttcagtt atctgaggt actcgttcg aggccctag 
      tcttagtca tgttgaggg gaagaaagt tgcaatgtc 
      ccgaagaag aggaatttt gcatattta ccgacttgg 
      atgaattta tcgaaataa tggcaatga ataattcgg 
      ttatttggg ttgatattg tcgcactta tcatatttt 
      tggtgcttg ttagaagtg ccagctttt atttattga 
      ggcagaggc tcacaccta acaagactt tcatggcct 
      tacagtata tcggtaggt ttattaaga cgcgaatga 
      gcgaaggtc gttaatgac agtgatata ggttagtgt 
      ggattttgt attgttaat taaatagag ctttatttt