| General information | |||
| TC007466 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 4082376..4091666 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007466 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01196 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
remarks: later than schedule, hours 96
- >TC007466 RA
MGRNFLLNN NRSSDYKVQ ITPTQQKTL THLPQRPKV DLAFSNLKY TVKQGKAEK TILKSVSGR LRSGELTAI MGPSGAGKS TLLNILTGY RTDGVQGQI LMNDSERDL SQFRKLSAY IMQDNQLHL NLTVDEAMN VAAKLKIGE KSKSEREDI ITEILDTLG LLDHRKTMT SGLSGGQKK RLSIALELV SNPPIMFFD EPTSGLDSS SCFQCISLL KTLARGGRT IICTIHQPS ARLFEMFDH LYTLADGQC VYQGSTKQL VPFLATLGL QCPSYHNPA SYIIEVACG EYGDHTRKL VEAIDNGKN DIREGKPFP EIKITNGLN NSTVITNDI SKGYANHAT ENKNLMLQN AVNDIAKEN LPAPPPITT NYEKANADT ALLENAIPA RQPRYGNSE FQQFFIILS RALLFSRRD WTLMYLRLF AHILVGFLI GALYFKIGN DGAKVLSNL GFLFFNMLF LMYTSMTIT ILSFPLEMP VLLKEHFNR WYSLRSYYL AITISDIPF QTIFCVLYV TIVYFMTSQ PPELNRYLM FLGACLLVS FVAQSVGLV VGAAMNVQN GVFLAPVMS VPFLLFSGF FVSFDAIPI YLRWITYLS YIRYGFEGT ALATYGFDR PKLQCFVSY CHFKSPTTT LEELDMIHS NYMIDIVAL VAIFFILRI AAYMFLKWK LKSTR
- >TC007466 RA
atggggcgc aatttcctt ctcaacaac aatcggagc tccgactac aaagtgcaa atcacaccg acacaacag aagacgctg acacatctt ccacaaagg cctaaagtc gatctagct ttttccaac ctcaaatac accgtcaaa caaggcaaa gccgaaaag actattctc aagtctgtg agcgggcgg ctgcgatct ggcgaactc acggcgatc atgggcccc tcgggggcc ggcaagtcc acccttctc aacatcctc acggggtac agaacggac ggagtgcag ggccaaatc ctgatgaac gacagcgag cgtgatttg agccaattt cgcaaattg tccgcttac ataatgcaa gacaatcaa ctgcacctc aacctaact gtggacgaa gcgatgaac gtcgctgcc aaactcaaa atcggcgaa aaatcaaaa tccgagcgt gaagacatc atcaccgaa atcctcgac actctcggc ctgctggat catcgcaaa acgatgacc agtggtctg tcgggtggt cagaaaaaa cgtctctcg atcgcctta gagctggtc agcaatccc cccatcatg ttctttgat gaacccacc agcggcctt gacagctcc tcgtgcttc cagtgtatt tcactattg aagactctc gcacgagga gggaggacc atcatttgc accatccac cagccttcg gcgaggcta ttcgagatg ttcgaccat ttgtatacc cttgccgat gggcagtgc gtgtaccaa ggctccacg aagcagttg gtgccgttc ttggccacg ctgggactc caatgccca tcgtaccac aacccagcg tcgtacatc atcgaagtg gcctgtggc gagtacggt gaccacact aggaagctg gtcgaggcg attgataac gggaaaaat gatatcaga gaagggaaa cctttccca gagataaaa atcacaaac ggcttgaat aactcaact gttattacc aatgacata agcaaagga tatgcgaac cacgccacc gaaaacaaa aatcttatg ctgcaaaat gccgttaac gacatcgcc aaagagaat ttgcctgcg cctccgccc atcaccaca aactacgaa aaggccaac gctgatacg gccctttta gagaatgcc attccggcc cgtcagccc cgctacggt aattccgaa tttcagcag tttttcatc attttgagt cgtgccttg ctgttcagc agacgtgat tggactttg atgtatctg cgcttgttt gcgcacatc ctggtcggt ttcttgatc ggtgctttg tatttcaaa attggcaat gacggtgct aaggtcctc agtaattta ggatttttg ttcttcaat atgttgttc ttaatgtac acttcaatg accatcacg atcttatca ttccctcta gaaatgccc gttttgctt aaagaacac ttcaatcgg tggtattcg ctcagatcg tactatcta gccattacg atatcagat ataccattc caaacaatt ttctgcgta ctgtacgta acaatagtc tactttatg acgtcccag ccgcctgaa ttaaaccgc tatttgatg tttttgggc gcttgttta ctcgtttct ttcgtagca caaagcgtt ggactcgtc gtaggggct gcaatgaat gtacagaac ggcgttttc ttggcaccg gtcatgtcg gtccctttc ttgctcttc tccgggttc tttgtctcc ttcgacgcc attccgatt tacctgcgc tggatcacc tacctgtcc tacatccgc tacgggttt gagggcaca gccctggcc acttacggc ttcgacagg ccgaagttg caatgtttc gtgtcttac tgtcacttt aaatcgccc accaccact ctggaagaa ctggacatg attcactca aactacatg attgatatt gtggccctt gtggcgatt ttcttcatt ctgagaata gccgcttac atgttcttg aagtggaag ctgaagtcg actcgttga
- >TC007466 RA
ctttcatcg ggctataat ttcacgcta actcagtcg agttaaact cttcgccac gatatgaaa taagtgcca gtgcatacg gatttttac atttgggca acatggggc gcaatttcc ttctcaaca acaatcgga gctccgact acaaagtgc aaatcacac cgacacaac agaagacgc tgacacatc ttccacaaa ggcctaaag tcgatctag ctttttcca acctcaaat acaccgtca aacaaggca aagccgaaa agactattc tcaagtctg tgagcgggc ggctgcgat ctggcgaac tcacggcga tcatgggcc cctcggggg ccggcaagt ccacccttc tcaacatcc tcacggggt acagaacgg acggagtgc agggccaaa tcctgatga acgacagcg agcgtgatt tgagccaat ttcgcaaat tgtccgctt acataatgc aagacaatc aactgcacc tcaacctaa ctgtggacg aagcgatga acgtcgctg ccaaactca aaatcggcg aaaaatcaa aatccgagc gtgaagaca tcatcaccg aaatcctcg acactctcg gcctgctgg atcatcgca aaacgatga ccagtggtc tgtcgggtg gtcagaaaa aacgtctct cgatcgcct tagagctgg tcagcaatc cccccatca tgttctttg atgaaccca ccagcggcc ttgacagct cctcgtgct tccagtgta tttcactat tgaagactc tcgcacgag gagggagga ccatcattt gcaccatcc accagcctt cggcgaggc tattcgaga tgttcgacc atttgtata cccttgccg atgggcagt gcgtgtacc aaggctcca cgaagcagt tggtgccgt tcttggcca cgctgggac tccaatgcc catcgtacc acaacccag cgtcgtaca tcatcgaag tggcctgtg gcgagtacg gtgaccaca ctaggaagc tggtcgagg cgattgata acgggaaaa atgatatca gagaaggga aacctttcc cagagataa aaatcacaa acggcttga ataactcaa ctgttatta ccaatgaca taagcaaag gatatgcga accacgcca ccgaaaaca aaaatctta tgctgcaaa atgccgtta acgacatcg ccaaagaga atttgcctg cgcctccgc ccatcacca caaactacg aaaaggcca acgctgata cggcccttt tagagaatg ccattccgg cccgtcagc cccgctacg gtaattccg aatttcagc agtttttca tcattttga gtcgtgcct tgctgttca gcagacgtg attggactt tgatgtatc tgcgcttgt ttgcgcaca tcctggtcg gtttcttga tcggtgctt tgtatttca aaattggca atgacggtg ctaaggtcc tcagtaatt taggatttt tgttcttca atatgttgt tcttaatgt acacttcaa tgaccatca cgatcttat cattccctc tagaaatgc ccgttttgc ttaaagaac acttcaatc ggtggtatt cgctcagat cgtactatc tagccatta cgatatcag atataccat tccaaacaa ttttctgcg tactgtacg taacaatag tctacttta tgacgtccc agccgcctg aattaaacc gctatttga tgtttttgg gcgcttgtt tactcgttt ctttcgtag cacaaagcg ttggactcg tcgtagggg ctgcaatga atgtacaga acggcgttt tcttggcac cggtcatgt cggtccctt tcttgctct tctccgggt tctttgtct ccttcgacg ccattccga tttacctgc gctggatca cctacctgt cctacatcc gctacgggt ttgagggca cagccctgg ccacttacg gcttcgaca ggccgaagt tgcaatgtt tcgtgtctt actgtcact ttaaatcgc ccaccacca ctctggaag aactggaca tgattcact caaactaca tgattgata ttgtggccc ttgtggcga ttttcttca ttctgagaa tagccgctt acatgttct tgaagtgga agctgaagt cgactcgtt gaatagtta attaagtgg tgatgcagc ggttcgtag cgtagtcat attgtgtcc aagttattg ttatttcat atctaacaa tttatttaa cacgtgtga tagtagata ggtagggct gtgattgtc tgttattta taaaaagaa atttttgaa taggagaaa cttgccaga atttgccca tgactggtt cccttcgat aaaggaaaa ttgtttagg tactcttgg accatcata cgatttata ttttttata cgctaatta tagagatat tttaatact tatataccg tatcaatta tgtacaaaa aatgtttaa taaattgtt taaaagtta
