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Gene information
General information
TC007466 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4082376..4091666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007466
    iBeetle screen iB_01196

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007466 RA
      MGRNFLLNN NRSSDYKVQ ITPTQQKTL THLPQRPKV 
DLAFSNLKY TVKQGKAEK TILKSVSGR LRSGELTAI 
MGPSGAGKS TLLNILTGY RTDGVQGQI LMNDSERDL 
SQFRKLSAY IMQDNQLHL NLTVDEAMN VAAKLKIGE 
KSKSEREDI ITEILDTLG LLDHRKTMT SGLSGGQKK 
RLSIALELV SNPPIMFFD EPTSGLDSS SCFQCISLL 
KTLARGGRT IICTIHQPS ARLFEMFDH LYTLADGQC 
VYQGSTKQL VPFLATLGL QCPSYHNPA SYIIEVACG 
EYGDHTRKL VEAIDNGKN DIREGKPFP EIKITNGLN 
NSTVITNDI SKGYANHAT ENKNLMLQN AVNDIAKEN 
LPAPPPITT NYEKANADT ALLENAIPA RQPRYGNSE 
FQQFFIILS RALLFSRRD WTLMYLRLF AHILVGFLI 
GALYFKIGN DGAKVLSNL GFLFFNMLF LMYTSMTIT 
ILSFPLEMP VLLKEHFNR WYSLRSYYL AITISDIPF 
QTIFCVLYV TIVYFMTSQ PPELNRYLM FLGACLLVS 
FVAQSVGLV VGAAMNVQN GVFLAPVMS VPFLLFSGF 
FVSFDAIPI YLRWITYLS YIRYGFEGT ALATYGFDR 
PKLQCFVSY CHFKSPTTT LEELDMIHS NYMIDIVAL 
VAIFFILRI AAYMFLKWK LKSTR
    CDS sequence
    • >TC007466 RA
      atggggcgc aatttcctt ctcaacaac aatcggagc 
tccgactac aaagtgcaa atcacaccg acacaacag 
aagacgctg acacatctt ccacaaagg cctaaagtc 
gatctagct ttttccaac ctcaaatac accgtcaaa 
caaggcaaa gccgaaaag actattctc aagtctgtg 
agcgggcgg ctgcgatct ggcgaactc acggcgatc 
atgggcccc tcgggggcc ggcaagtcc acccttctc 
aacatcctc acggggtac agaacggac ggagtgcag 
ggccaaatc ctgatgaac gacagcgag cgtgatttg 
agccaattt cgcaaattg tccgcttac ataatgcaa 
gacaatcaa ctgcacctc aacctaact gtggacgaa 
gcgatgaac gtcgctgcc aaactcaaa atcggcgaa 
aaatcaaaa tccgagcgt gaagacatc atcaccgaa 
atcctcgac actctcggc ctgctggat catcgcaaa 
acgatgacc agtggtctg tcgggtggt cagaaaaaa 
cgtctctcg atcgcctta gagctggtc agcaatccc 
cccatcatg ttctttgat gaacccacc agcggcctt 
gacagctcc tcgtgcttc cagtgtatt tcactattg 
aagactctc gcacgagga gggaggacc atcatttgc 
accatccac cagccttcg gcgaggcta ttcgagatg 
ttcgaccat ttgtatacc cttgccgat gggcagtgc 
gtgtaccaa ggctccacg aagcagttg gtgccgttc 
ttggccacg ctgggactc caatgccca tcgtaccac 
aacccagcg tcgtacatc atcgaagtg gcctgtggc 
gagtacggt gaccacact aggaagctg gtcgaggcg 
attgataac gggaaaaat gatatcaga gaagggaaa 
cctttccca gagataaaa atcacaaac ggcttgaat 
aactcaact gttattacc aatgacata agcaaagga 
tatgcgaac cacgccacc gaaaacaaa aatcttatg 
ctgcaaaat gccgttaac gacatcgcc aaagagaat 
ttgcctgcg cctccgccc atcaccaca aactacgaa 
aaggccaac gctgatacg gccctttta gagaatgcc 
attccggcc cgtcagccc cgctacggt aattccgaa 
tttcagcag tttttcatc attttgagt cgtgccttg 
ctgttcagc agacgtgat tggactttg atgtatctg 
cgcttgttt gcgcacatc ctggtcggt ttcttgatc 
ggtgctttg tatttcaaa attggcaat gacggtgct 
aaggtcctc agtaattta ggatttttg ttcttcaat 
atgttgttc ttaatgtac acttcaatg accatcacg 
atcttatca ttccctcta gaaatgccc gttttgctt 
aaagaacac ttcaatcgg tggtattcg ctcagatcg 
tactatcta gccattacg atatcagat ataccattc 
caaacaatt ttctgcgta ctgtacgta acaatagtc 
tactttatg acgtcccag ccgcctgaa ttaaaccgc 
tatttgatg tttttgggc gcttgttta ctcgtttct 
ttcgtagca caaagcgtt ggactcgtc gtaggggct 
gcaatgaat gtacagaac ggcgttttc ttggcaccg 
gtcatgtcg gtccctttc ttgctcttc tccgggttc 
tttgtctcc ttcgacgcc attccgatt tacctgcgc 
tggatcacc tacctgtcc tacatccgc tacgggttt 
gagggcaca gccctggcc acttacggc ttcgacagg 
ccgaagttg caatgtttc gtgtcttac tgtcacttt 
aaatcgccc accaccact ctggaagaa ctggacatg 
attcactca aactacatg attgatatt gtggccctt 
gtggcgatt ttcttcatt ctgagaata gccgcttac 
atgttcttg aagtggaag ctgaagtcg actcgttga
    mRNA sequence
    • >TC007466 RA
      ctttcatcg ggctataat ttcacgcta actcagtcg 
agttaaact cttcgccac gatatgaaa taagtgcca 
gtgcatacg gatttttac atttgggca acatggggc 
gcaatttcc ttctcaaca acaatcgga gctccgact 
acaaagtgc aaatcacac cgacacaac agaagacgc 
tgacacatc ttccacaaa ggcctaaag tcgatctag 
ctttttcca acctcaaat acaccgtca aacaaggca 
aagccgaaa agactattc tcaagtctg tgagcgggc 
ggctgcgat ctggcgaac tcacggcga tcatgggcc 
cctcggggg ccggcaagt ccacccttc tcaacatcc 
tcacggggt acagaacgg acggagtgc agggccaaa 
tcctgatga acgacagcg agcgtgatt tgagccaat 
ttcgcaaat tgtccgctt acataatgc aagacaatc 
aactgcacc tcaacctaa ctgtggacg aagcgatga 
acgtcgctg ccaaactca aaatcggcg aaaaatcaa 
aatccgagc gtgaagaca tcatcaccg aaatcctcg 
acactctcg gcctgctgg atcatcgca aaacgatga 
ccagtggtc tgtcgggtg gtcagaaaa aacgtctct 
cgatcgcct tagagctgg tcagcaatc cccccatca 
tgttctttg atgaaccca ccagcggcc ttgacagct 
cctcgtgct tccagtgta tttcactat tgaagactc 
tcgcacgag gagggagga ccatcattt gcaccatcc 
accagcctt cggcgaggc tattcgaga tgttcgacc 
atttgtata cccttgccg atgggcagt gcgtgtacc 
aaggctcca cgaagcagt tggtgccgt tcttggcca 
cgctgggac tccaatgcc catcgtacc acaacccag 
cgtcgtaca tcatcgaag tggcctgtg gcgagtacg 
gtgaccaca ctaggaagc tggtcgagg cgattgata 
acgggaaaa atgatatca gagaaggga aacctttcc 
cagagataa aaatcacaa acggcttga ataactcaa 
ctgttatta ccaatgaca taagcaaag gatatgcga 
accacgcca ccgaaaaca aaaatctta tgctgcaaa 
atgccgtta acgacatcg ccaaagaga atttgcctg 
cgcctccgc ccatcacca caaactacg aaaaggcca 
acgctgata cggcccttt tagagaatg ccattccgg 
cccgtcagc cccgctacg gtaattccg aatttcagc 
agtttttca tcattttga gtcgtgcct tgctgttca 
gcagacgtg attggactt tgatgtatc tgcgcttgt 
ttgcgcaca tcctggtcg gtttcttga tcggtgctt 
tgtatttca aaattggca atgacggtg ctaaggtcc 
tcagtaatt taggatttt tgttcttca atatgttgt 
tcttaatgt acacttcaa tgaccatca cgatcttat 
cattccctc tagaaatgc ccgttttgc ttaaagaac 
acttcaatc ggtggtatt cgctcagat cgtactatc 
tagccatta cgatatcag atataccat tccaaacaa 
ttttctgcg tactgtacg taacaatag tctacttta 
tgacgtccc agccgcctg aattaaacc gctatttga 
tgtttttgg gcgcttgtt tactcgttt ctttcgtag 
cacaaagcg ttggactcg tcgtagggg ctgcaatga 
atgtacaga acggcgttt tcttggcac cggtcatgt 
cggtccctt tcttgctct tctccgggt tctttgtct 
ccttcgacg ccattccga tttacctgc gctggatca 
cctacctgt cctacatcc gctacgggt ttgagggca 
cagccctgg ccacttacg gcttcgaca ggccgaagt 
tgcaatgtt tcgtgtctt actgtcact ttaaatcgc 
ccaccacca ctctggaag aactggaca tgattcact 
caaactaca tgattgata ttgtggccc ttgtggcga 
ttttcttca ttctgagaa tagccgctt acatgttct 
tgaagtgga agctgaagt cgactcgtt gaatagtta 
attaagtgg tgatgcagc ggttcgtag cgtagtcat 
attgtgtcc aagttattg ttatttcat atctaacaa 
tttatttaa cacgtgtga tagtagata ggtagggct 
gtgattgtc tgttattta taaaaagaa atttttgaa 
taggagaaa cttgccaga atttgccca tgactggtt 
cccttcgat aaaggaaaa ttgtttagg tactcttgg 
accatcata cgatttata ttttttata cgctaatta 
tagagatat tttaatact tatataccg tatcaatta 
tgtacaaaa aatgtttaa taaattgtt taaaagtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY