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Gene information
General information
TC007466 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 4082376..4091666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007466
    iBeetle screen iB_01196
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007466 RA
      MGRNFLLNN NRSSDYKVQ ITPTQQKTL THLPQRPKV 
      DLAFSNLKY TVKQGKAEK TILKSVSGR LRSGELTAI 
      MGPSGAGKS TLLNILTGY RTDGVQGQI LMNDSERDL 
      SQFRKLSAY IMQDNQLHL NLTVDEAMN VAAKLKIGE 
      KSKSEREDI ITEILDTLG LLDHRKTMT SGLSGGQKK 
      RLSIALELV SNPPIMFFD EPTSGLDSS SCFQCISLL 
      KTLARGGRT IICTIHQPS ARLFEMFDH LYTLADGQC 
      VYQGSTKQL VPFLATLGL QCPSYHNPA SYIIEVACG 
      EYGDHTRKL VEAIDNGKN DIREGKPFP EIKITNGLN 
      NSTVITNDI SKGYANHAT ENKNLMLQN AVNDIAKEN 
      LPAPPPITT NYEKANADT ALLENAIPA RQPRYGNSE 
      FQQFFIILS RALLFSRRD WTLMYLRLF AHILVGFLI 
      GALYFKIGN DGAKVLSNL GFLFFNMLF LMYTSMTIT 
      ILSFPLEMP VLLKEHFNR WYSLRSYYL AITISDIPF 
      QTIFCVLYV TIVYFMTSQ PPELNRYLM FLGACLLVS 
      FVAQSVGLV VGAAMNVQN GVFLAPVMS VPFLLFSGF 
      FVSFDAIPI YLRWITYLS YIRYGFEGT ALATYGFDR 
      PKLQCFVSY CHFKSPTTT LEELDMIHS NYMIDIVAL 
      VAIFFILRI AAYMFLKWK LKSTR
    CDS sequence
    • >TC007466 RA
      atggggcgc aatttcctt ctcaacaac aatcggagc 
      tccgactac aaagtgcaa atcacaccg acacaacag 
      aagacgctg acacatctt ccacaaagg cctaaagtc 
      gatctagct ttttccaac ctcaaatac accgtcaaa 
      caaggcaaa gccgaaaag actattctc aagtctgtg 
      agcgggcgg ctgcgatct ggcgaactc acggcgatc 
      atgggcccc tcgggggcc ggcaagtcc acccttctc 
      aacatcctc acggggtac agaacggac ggagtgcag 
      ggccaaatc ctgatgaac gacagcgag cgtgatttg 
      agccaattt cgcaaattg tccgcttac ataatgcaa 
      gacaatcaa ctgcacctc aacctaact gtggacgaa 
      gcgatgaac gtcgctgcc aaactcaaa atcggcgaa 
      aaatcaaaa tccgagcgt gaagacatc atcaccgaa 
      atcctcgac actctcggc ctgctggat catcgcaaa 
      acgatgacc agtggtctg tcgggtggt cagaaaaaa 
      cgtctctcg atcgcctta gagctggtc agcaatccc 
      cccatcatg ttctttgat gaacccacc agcggcctt 
      gacagctcc tcgtgcttc cagtgtatt tcactattg 
      aagactctc gcacgagga gggaggacc atcatttgc 
      accatccac cagccttcg gcgaggcta ttcgagatg 
      ttcgaccat ttgtatacc cttgccgat gggcagtgc 
      gtgtaccaa ggctccacg aagcagttg gtgccgttc 
      ttggccacg ctgggactc caatgccca tcgtaccac 
      aacccagcg tcgtacatc atcgaagtg gcctgtggc 
      gagtacggt gaccacact aggaagctg gtcgaggcg 
      attgataac gggaaaaat gatatcaga gaagggaaa 
      cctttccca gagataaaa atcacaaac ggcttgaat 
      aactcaact gttattacc aatgacata agcaaagga 
      tatgcgaac cacgccacc gaaaacaaa aatcttatg 
      ctgcaaaat gccgttaac gacatcgcc aaagagaat 
      ttgcctgcg cctccgccc atcaccaca aactacgaa 
      aaggccaac gctgatacg gccctttta gagaatgcc 
      attccggcc cgtcagccc cgctacggt aattccgaa 
      tttcagcag tttttcatc attttgagt cgtgccttg 
      ctgttcagc agacgtgat tggactttg atgtatctg 
      cgcttgttt gcgcacatc ctggtcggt ttcttgatc 
      ggtgctttg tatttcaaa attggcaat gacggtgct 
      aaggtcctc agtaattta ggatttttg ttcttcaat 
      atgttgttc ttaatgtac acttcaatg accatcacg 
      atcttatca ttccctcta gaaatgccc gttttgctt 
      aaagaacac ttcaatcgg tggtattcg ctcagatcg 
      tactatcta gccattacg atatcagat ataccattc 
      caaacaatt ttctgcgta ctgtacgta acaatagtc 
      tactttatg acgtcccag ccgcctgaa ttaaaccgc 
      tatttgatg tttttgggc gcttgttta ctcgtttct 
      ttcgtagca caaagcgtt ggactcgtc gtaggggct 
      gcaatgaat gtacagaac ggcgttttc ttggcaccg 
      gtcatgtcg gtccctttc ttgctcttc tccgggttc 
      tttgtctcc ttcgacgcc attccgatt tacctgcgc 
      tggatcacc tacctgtcc tacatccgc tacgggttt 
      gagggcaca gccctggcc acttacggc ttcgacagg 
      ccgaagttg caatgtttc gtgtcttac tgtcacttt 
      aaatcgccc accaccact ctggaagaa ctggacatg 
      attcactca aactacatg attgatatt gtggccctt 
      gtggcgatt ttcttcatt ctgagaata gccgcttac 
      atgttcttg aagtggaag ctgaagtcg actcgttga
    mRNA sequence
    • >TC007466 RA
      ctttcatcg ggctataat ttcacgcta actcagtcg 
      agttaaact cttcgccac gatatgaaa taagtgcca 
      gtgcatacg gatttttac atttgggca acatggggc 
      gcaatttcc ttctcaaca acaatcgga gctccgact 
      acaaagtgc aaatcacac cgacacaac agaagacgc 
      tgacacatc ttccacaaa ggcctaaag tcgatctag 
      ctttttcca acctcaaat acaccgtca aacaaggca 
      aagccgaaa agactattc tcaagtctg tgagcgggc 
      ggctgcgat ctggcgaac tcacggcga tcatgggcc 
      cctcggggg ccggcaagt ccacccttc tcaacatcc 
      tcacggggt acagaacgg acggagtgc agggccaaa 
      tcctgatga acgacagcg agcgtgatt tgagccaat 
      ttcgcaaat tgtccgctt acataatgc aagacaatc 
      aactgcacc tcaacctaa ctgtggacg aagcgatga 
      acgtcgctg ccaaactca aaatcggcg aaaaatcaa 
      aatccgagc gtgaagaca tcatcaccg aaatcctcg 
      acactctcg gcctgctgg atcatcgca aaacgatga 
      ccagtggtc tgtcgggtg gtcagaaaa aacgtctct 
      cgatcgcct tagagctgg tcagcaatc cccccatca 
      tgttctttg atgaaccca ccagcggcc ttgacagct 
      cctcgtgct tccagtgta tttcactat tgaagactc 
      tcgcacgag gagggagga ccatcattt gcaccatcc 
      accagcctt cggcgaggc tattcgaga tgttcgacc 
      atttgtata cccttgccg atgggcagt gcgtgtacc 
      aaggctcca cgaagcagt tggtgccgt tcttggcca 
      cgctgggac tccaatgcc catcgtacc acaacccag 
      cgtcgtaca tcatcgaag tggcctgtg gcgagtacg 
      gtgaccaca ctaggaagc tggtcgagg cgattgata 
      acgggaaaa atgatatca gagaaggga aacctttcc 
      cagagataa aaatcacaa acggcttga ataactcaa 
      ctgttatta ccaatgaca taagcaaag gatatgcga 
      accacgcca ccgaaaaca aaaatctta tgctgcaaa 
      atgccgtta acgacatcg ccaaagaga atttgcctg 
      cgcctccgc ccatcacca caaactacg aaaaggcca 
      acgctgata cggcccttt tagagaatg ccattccgg 
      cccgtcagc cccgctacg gtaattccg aatttcagc 
      agtttttca tcattttga gtcgtgcct tgctgttca 
      gcagacgtg attggactt tgatgtatc tgcgcttgt 
      ttgcgcaca tcctggtcg gtttcttga tcggtgctt 
      tgtatttca aaattggca atgacggtg ctaaggtcc 
      tcagtaatt taggatttt tgttcttca atatgttgt 
      tcttaatgt acacttcaa tgaccatca cgatcttat 
      cattccctc tagaaatgc ccgttttgc ttaaagaac 
      acttcaatc ggtggtatt cgctcagat cgtactatc 
      tagccatta cgatatcag atataccat tccaaacaa 
      ttttctgcg tactgtacg taacaatag tctacttta 
      tgacgtccc agccgcctg aattaaacc gctatttga 
      tgtttttgg gcgcttgtt tactcgttt ctttcgtag 
      cacaaagcg ttggactcg tcgtagggg ctgcaatga 
      atgtacaga acggcgttt tcttggcac cggtcatgt 
      cggtccctt tcttgctct tctccgggt tctttgtct 
      ccttcgacg ccattccga tttacctgc gctggatca 
      cctacctgt cctacatcc gctacgggt ttgagggca 
      cagccctgg ccacttacg gcttcgaca ggccgaagt 
      tgcaatgtt tcgtgtctt actgtcact ttaaatcgc 
      ccaccacca ctctggaag aactggaca tgattcact 
      caaactaca tgattgata ttgtggccc ttgtggcga 
      ttttcttca ttctgagaa tagccgctt acatgttct 
      tgaagtgga agctgaagt cgactcgtt gaatagtta 
      attaagtgg tgatgcagc ggttcgtag cgtagtcat 
      attgtgtcc aagttattg ttatttcat atctaacaa 
      tttatttaa cacgtgtga tagtagata ggtagggct 
      gtgattgtc tgttattta taaaaagaa atttttgaa 
      taggagaaa cttgccaga atttgccca tgactggtt 
      cccttcgat aaaggaaaa ttgtttagg tactcttgg 
      accatcata cgatttata ttttttata cgctaatta 
      tagagatat tttaatact tatataccg tatcaatta 
      tgtacaaaa aatgtttaa taaattgtt taaaagtta