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Gene information
General information
TC007450 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 4310811..4316048
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007450
      iBeetle screen iB_01193
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Fertility
      previtellogenic egg chamber complete not present - Number of animals showing the phenotype: 4
      vitellogenic egg chamber size decreased - Number of animals showing the phenotype: 4
      vitellogenic egg chamber shape rounded - Number of animals showing the phenotype: 4
      tropharium irregular - Number of animals showing the phenotype: 4

      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      Fertility
      egg production: number <50% of expected -
      ovariole shape irregular - Number of animals showing the phenotype: 3

      vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

      previtellogenic egg chamber potentially number increased - Number of animals showing the phenotype: 3

      tropharium structure irregular - Number of animals showing the phenotype: 3

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007450 RA
        MGHIFRCSH AKTQSCVFK PFGSFLNPD VNDENMRTL 
        SLHFGAIAL ASSATMQPI NNNKRNEIA NIDLAILRA 
        PLTGDLCLF RDLDFGAFC RTGGWFNGL EVVSGSFDN 
        FHDFAFAVD DHRTGRTPN NVTLGVQRS LFAEIEVPF 
        GAILGHVVV EVILDEGLV VLGVDLPEE TAFHFSDGA 
        LAPHEFVAH FDVFANAHT HDRQPTIHE SKPRFTLPN 
        QKTLNSGVE NRFSRRRGT GECPYGTRG ESHSGFVQV 
        SARKSQRRF EQTHTSFRL GRLYATDAR KRRYGIVWM 
        CWTVPPVVD SPIHTKITI FALPKP
      CDS sequence
      • >TC007450 RA
        atgggtcac atatttcgg tgttcacac gcaaaaacc 
        cagtcatgt gtcttcaag cctttcggc agtttcctc 
        aacccggat gtcaacgac gaaaacatg agaactctt 
        tcgctgcat ttcggggcc attgctctc gccagctcg 
        gcaacaatg caaccaata aacaataat aaacgcaac 
        gaaatcgca aatattgac ttagccatt ttgagagcg 
        ccactcacc ggtgatttg tgtctgttt cgggacctt 
        gattttggg gctttctgt cgaacgggc ggctggttc 
        aatgggctg gaagtagta agcgggagc ttcgacaat 
        ttccacgat tttgcgttt gcggttgac gatcaccgg 
        actggcaga acgccaaat aatgtcacc ctcggggtc 
        cagcgtagt ctcttcgcg gaaattgaa gttccattt 
        ggcgcaatt cttggacat gtcgtagtt gaggtgatt 
        ctggatgaa ggccttgtt gtcctcggg gtcgacctt 
        cccgaagag actgcgttt cactttagc gatggggct 
        cttgcgccc catgaattt gtagctcat tttgacgtt 
        ttcgcaaac gctcatact catgatagg cagccaaca 
        atacacgag tccaagcca cgcttcact ttaccaaac 
        cagaaaaca ctcaattcc ggtgtggaa aaccgattt 
        tcccggagg agaggcacg ggtgagtgt ccgtatggc 
        acacggggt gagagtcac agcgggttt gtgcaagtt 
        tccgcgaga aaatcccaa cgaagattc gaacaaacg 
        cacacaagc tttcgttta ggacgactt tacgcgact 
        gacgcgagg aaaagacgc tatggaatc gtgtggatg 
        tgttggacc gttccaccg gtagttgac tctcctatt 
        cacacaaaa attaccatt ttcgccctg ccaaaacct 
        tga
      mRNA sequence
      • >TC007450 RA
        atgggtcac atatttcgg tgttcacac gcaaaaacc 
        cagtcatgt gtcttcaag cctttcggc agtttcctc 
        aacccggat gtcaacgac gaaaacatg agaactctt 
        tcgctgcat ttcggggcc attgctctc gccagctcg 
        gcaacaatg caaccaata aacaataat aaacgcaac 
        gaaatcgca aatattgac ttagccatt ttgagagcg 
        ccactcacc ggtgatttg tgtctgttt cgggacctt 
        gattttggg gctttctgt cgaacgggc ggctggttc 
        aatgggctg gaagtagta agcgggagc ttcgacaat 
        ttccacgat tttgcgttt gcggttgac gatcaccgg 
        actggcaga acgccaaat aatgtcacc ctcggggtc 
        cagcgtagt ctcttcgcg gaaattgaa gttccattt 
        ggcgcaatt cttggacat gtcgtagtt gaggtgatt 
        ctggatgaa ggccttgtt gtcctcggg gtcgacctt 
        cccgaagag actgcgttt cactttagc gatggggct 
        cttgcgccc catgaattt gtagctcat tttgacgtt 
        ttcgcaaac gctcatact catgatagg cagccaaca 
        atacacgag tccaagcca cgcttcact ttaccaaac 
        cagaaaaca ctcaattcc ggtgtggaa aaccgattt 
        tcccggagg agaggcacg ggtgagtgt ccgtatggc 
        acacggggt gagagtcac agcgggttt gtgcaagtt 
        tccgcgaga aaatcccaa cgaagattc gaacaaacg 
        cacacaagc tttcgttta ggacgactt tacgcgact 
        gacgcgagg aaaagacgc tatggaatc gtgtggatg 
        tgttggacc gttccaccg gtagttgac tctcctatt 
        cacacaaaa attaccatt ttcgccctg ccaaaacct 
        tga