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Gene information
General information
TC007447 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4348153..4350530
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007447
    iBeetle screen iB_01192

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007447 RA
      MLFNGKKVF YLFIITALN LASGIRTYT KNDLIKLRE 
      EVREMFYHA YDSYLKYAY PYDELRPIS CDGIDTYGS 
      YSLTLIDAL GTLAIMGNH SEFQRVVQI LTAKPDFDA 
      NINVSVFET NIRIVGGLL SAHLLSHRA GVKLEPGWP 
      CNGPLLRLA EDVAKRLIV AFDTKTGMP YGTINLRKG 
      VPKGETAVT CTAGIGTFI LEFGTLSRL TGDPLYEEV 
      ALNALYALY NHKSPLGLY GNHIDVETG RWTYQDAGI 
      GSGVDSFFE YLVKGSILL QRPELLEIF HEARKSIDK 
      YLKKDDWYM WVSMTKGQV TIPVFQSLE AYWPGVLSL 
      IGDTSSAMK TLHNYHQVW LQYGFTPEF YYIPQSEAG 
      TNRESYPLR PELIESIMY LYRATEDPF LLQAGEDIL 
      RSIQHSART PCGYATIKD VRDHRKEDR MESFFLSET 
      TKYLYLLFD TENFIHNQG QHGTVISTP NGECLIETG 
      GYIFNTEAH PLDPAALHC CHDVPKLNF FNFDDFEEK 
      KSLFRGDSI TERRNYINK NDCPESEDD DKLVLSNKT 
      EEIAVEEIT TESSNSYVK LEDNDNVSQ KINFEENVT 
      LKNEEYRKK SSKKFDEQE MLERFRAES KNNRNATWE 
      TNYKLLTCK AQPFLQKLS ILGEFFKYI
    CDS sequence
    • >TC007447 RA
      atgttattt aacggcaaa aaagtgttt tacttattc 
      ataatcacg gctcttaat ttggcaagc ggcatccgg 
      acctacaca aaaaatgat ttaatcaaa ttgagagag 
      gaagtaaga gaaatgttt tatcacgcc tacgacagc 
      tatttaaaa tatgcgtac ccctacgac gagcttagg 
      cccataagt tgcgacgga atcgatact tatggaagt 
      tattcgctc acgttgatt gacgctttg gggacactg 
      gcaataatg gggaaccac tcagagttt caaagagtg 
      gtccaaatt ttaacggcc aagcctgac tttgatgca 
      aacatcaac gtttcagtt tttgagacg aatattaga 
      atagttggg gggttacta agtgcgcat ttattgtcc 
      catagggca ggtgttaag ctggagcct ggttggcca 
      tgcaatggc cctttactc agactagct gaagacgtg 
      gccaaacgt ttaatcgta gcctttgac actaaaaca 
      gggatgcct tacggcacc attaacttg cgaaagggc 
      gtccctaag ggcgagacc gcagtcacg tgcactgcc 
      ggcataggg acttttatt ttggagttt ggtactttg 
      agtagactc acgggggat cccctttac gaagaagtt 
      gcactgaac gctctctac gccctttac aatcacaag 
      tcaccgtta ggcctttac ggcaatcac attgacgta 
      gagacgggg cgttggacc tatcaggac gcagggata 
      ggttctggg gtcgactca tttttcgag tatttggtc 
      aagggttcg attttgttg caacgaccg gaattgtta 
      gaaatattt cacgaagcc aggaaatcg attgataaa 
      tatttgaaa aaggacgac tggtacatg tgggtttct 
      atgaccaaa gggcaggtc acgatcccc gtatttcaa 
      tcattggag gcctactgg cctggagtt ttgagtctt 
      attggtgac acaagttct gcaatgaag acgctccac 
      aattaccac caagtgtgg ctccagtat ggtttcacg 
      cccgaattt tattacatt ccgcagtca gaagccggg 
      actaatagg gagagctac cctcttagg cccgagttg 
      attgaatca attatgtac ctgtatagg gccacagaa 
      gacccgttt ttgctccaa gctggtgaa gatatcctg 
      cgaagtatt caacacagc gcaaggaca ccttgtggc 
      tatgccact attaaggac gtccgggat catagaaag 
      gaagacagg atggaatca ttcttcctt tctgagacc 
      acgaaatat ttgtatctt ctttttgac accgagaat 
      tttattcac aatcaaggc caacacggc actgtgatt 
      agcacccca aacggggaa tgtctcata gagacaggg 
      ggctatatt ttcaacact gaggcccac ccacttgac 
      cccgccgct ttgcattgt tgccacgac gtccccaaa 
      ttaaacttc ttcaatttc gatgatttt gaggagaag 
      aaaagtctt ttccgtggt gatagtatc acagaacgc 
      aggaattac ataaataaa aatgactgt cctgaatcg 
      gaagacgat gacaaactt gtcctaagt aataagact 
      gaggaaatt gccgtcgag gaaatcaca actgaatcg 
      agtaatagc tacgtcaag ttggaagac aacgataac 
      gtcagccag aaaatcaat ttcgaagaa aatgtgact 
      cttaaaaat gaagaatat agaaagaag tcgagcaaa 
      aaattcgac gagcaagaa atgttggag cgcttccgc 
      gccgaaagt aagaacaat cggaacgcc acgtgggaa 
      accaactac aagctgctc acatgcaag gcccagccg 
      ttcctccag aaactctca atcctcggc gaatttttc 
      aaatatatt taa
    mRNA sequence
    • >TC007447 RA
      tgtcaaaat tccaacctc aaccaaaat gtcaaaatt 
      caaccttac acgtaataa atggattta atcagattt 
      ttaaccctc tcaaaatgt tatttaacg gcaaaaaag 
      tgttttact tattcataa tcacggctc ttaatttgg 
      caagcggca tccggacct acacaaaaa atgatttaa 
      tcaaattga gagaggaag taagagaaa tgttttatc 
      acgcctacg acagctatt taaaatatg cgtacccct 
      acgacgagc ttaggccca taagttgcg acggaatcg 
      atacttatg gaagttatt cgctcacgt tgattgacg 
      ctttgggga cactggcaa taatgggga accactcag 
      agtttcaaa gagtggtcc aaattttaa cggccaagc 
      ctgactttg atgcaaaca tcaacgttt cagtttttg 
      agacgaata ttagaatag ttggggggt tactaagtg 
      cgcatttat tgtcccata gggcaggtg ttaagctgg 
      agcctggtt ggccatgca atggccctt tactcagac 
      tagctgaag acgtggcca aacgtttaa tcgtagcct 
      ttgacacta aaacaggga tgccttacg gcaccatta 
      acttgcgaa agggcgtcc ctaagggcg agaccgcag 
      tcacgtgca ctgccggca tagggactt ttattttgg 
      agtttggta ctttgagta gactcacgg gggatcccc 
      tttacgaag aagttgcac tgaacgctc tctacgccc 
      tttacaatc acaagtcac cgttaggcc tttacggca 
      atcacattg acgtagaga cggggcgtt ggacctatc 
      aggacgcag ggataggtt ctggggtcg actcatttt 
      tcgagtatt tggtcaagg gttcgattt tgttgcaac 
      gaccggaat tgttagaaa tatttcacg aagccagga 
      aatcgattg ataaatatt tgaaaaagg acgactggt 
      acatgtggg tttctatga ccaaagggc aggtcacga 
      tccccgtat ttcaatcat tggaggcct actggcctg 
      gagttttga gtcttattg gtgacacaa gttctgcaa 
      tgaagacgc tccacaatt accaccaag tgtggctcc 
      agtatggtt tcacgcccg aattttatt acattccgc 
      agtcagaag ccgggacta atagggaga gctaccctc 
      ttaggcccg agttgattg aatcaatta tgtacctgt 
      atagggcca cagaagacc cgtttttgc tccaagctg 
      gtgaagata tcctgcgaa gtattcaac acagcgcaa 
      ggacacctt gtggctatg ccactatta aggacgtcc 
      gggatcata gaaaggaag acaggatgg aatcattct 
      tcctttctg agaccacga aatatttgt atcttcttt 
      ttgacaccg agaatttta ttcacaatc aaggccaac 
      acggcactg tgattagca ccccaaacg gggaatgtc 
      tcatagaga cagggggct atattttca acactgagg 
      cccacccac ttgaccccg ccgctttgc attgttgcc 
      acgacgtcc ccaaattaa acttcttca atttcgatg 
      attttgagg agaagaaaa gtcttttcc gtggtgata 
      gtatcacag aacgcagga attacataa ataaaaatg 
      actgtcctg aatcggaag acgatgaca aacttgtcc 
      taagtaata agactgagg aaattgccg tcgaggaaa 
      tcacaactg aatcgagta atagctacg tcaagttgg 
      aagacaacg ataacgtca gccagaaaa tcaatttcg 
      aagaaaatg tgactctta aaaatgaag aatatagaa 
      agaagtcga gcaaaaaat tcgacgagc aagaaatgt 
      tggagcgct tccgcgccg aaagtaaga acaatcgga 
      acgccacgt gggaaacca actacaagc tgctcacat 
      gcaaggccc agccgttcc tccagaaac tctcaatcc 
      tcggcgaat ttttcaaat atatttaaa tttgttacg 
      ctgctaaat aacaaatca aataaataa acaacacta 
      cttcct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY