General information | |||
TC007439 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 4487263..4492410 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC007439 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01189 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC007439 RA
MTDNHEVPN PKDFLAQMG PIETLLGSL SYNDIDNVD GKTQQLRTS ARVFKKMKF DLSSVEKKV EKKEEVKPE EKQEVKKPA NPFWSSEEK NLFFEALNE YGKDFESIH GYISTRLKK KGVPEDLIK NKLQVRHFY YKTFHMLLG PLKFNEKIP KNVQELYTL INFGEMRRK LGSISKKNC SKLNDLIAN GSVPLRLKG KTVRVKTPM CRALRRLNQ LDEKYDDLK LPTRVLVEL RPKNMASFL RVQSVAQNP RIKVALSLQ KRLSSLITC INTRWKTSE AVVYDKAVI STNPVTKNC VPDTKTIDD STLLLNPLL RLSPPPEAH IELPSIDIS EYFNGQSIS LNAFELRKG VKKEEPEAP PKTEKSPNK PDEEEIPKS VTSVVDEAV NTILSLQVQ NKTDTKTAT TTNPKDIPN EEKISRIEQ IKQGWTLEN CASLTIGEI YLMYGCDSK MVLEYSWDC LIKSNDETK QENDLTNSL KKLLSLAKI QSEKPVMKC TCGHVCGKP STQQFKKPV APKVVKQKQ VSHETNHVQ EKVEVSEPK PAPEPKKAP PEEPFHDTS SSVVHLFSQ RDKFRIKGR TRFKHLVVE RKLVPNKSE NKHEIVSMD IVSQDKPDD MPLVNSSGL PSMLMGDND TRMSEKSFS GFLGDNQSN SAMSTGLSK ILKENENQW INADVADYS LSSLLGHLD CPSKTNQSM VSLSHDDTQ LSQDVDAQL RTLLIETSV DYTAKFADL AAQVVENKK
- >TC007439 RA
atgaccgac aaccatgag gtccccaac cctaaagac ttcctggcc cagatgggc cccatcgag acccttctg ggttcgctg tcttacaac gacatcgac aatgtcgat ggcaaaacc cagcaattg cgcaccagc gctcgcgtt ttcaaaaaa atgaaattc gacctctcc tccgtcgag aaaaaagtc gaaaagaag gaggaagtc aagccggag gagaagcaa gaagtgaag aaaccggcc aatccgttc tggtcgagc gaagagaag aatcttttc ttcgaagcg ttgaacgaa tacgggaag gattttgaa agcatccac gggtacatc agcacccgc ttgaagaag aaaggggtc ccggaggac ctcatcaag aacaagctg caagtcagg cacttttat tacaaaacc ttccacatg ttgttgggg ccgttgaaa tttaacgag aaaattccg aaaaacgtg caagagctg tacacgttg atcaatttt ggcgaaatg aggagaaaa cttggcagt atctcgaaa aaaaattgc tcgaaactg aacgatttg attgcaaac ggttcagtt cctttgagg cttaagggg aaaaccgtc agggttaaa actccaatg tgtagggct ttgagacgc ttgaaccaa ttagatgaa aaatacgat gatcttaaa ttgccaacg agggttttg gtcgagctc agaccaaaa aacatggct tcgtttctc agagtgcaa agtgttgct caaaatccg cgaatcaaa gtcgcactc tcgctacaa aagcggttg agctcgctt atcacgtgc ataaacacc cgctggaag acctccgag gccgttgtt tacgataaa gctgtgatt tcgacaaac ccggtaaca aaaaattgc gttcctgat acgaaaaca attgatgat agtacgttg ttactgaat ccgctctta aggctgtct cctccgccg gaggcccac attgagttg ccttcaatc gatatcagc gaatatttc aacggtcaa agcatatca cttaatgca ttcgaattg agaaaaggg gttaaaaag gaggagcct gaagcgccc cccaagact gaaaaatcg ccgaacaag cctgacgaa gaggaaatc cccaaatcg gtcaccagc gtcgtggac gaggcagtc aacacaatt ctctcgctg caagtccag aataaaacc gacaccaag actgccacc acaaccaac cctaaagat atccccaat gaggagaaa atatcaagg atcgagcaa atcaagcag gggtggact ttggagaat tgcgccagt ttgaccatt ggcgagatc tacttgatg tacgggtgt gactcaaaa atggtgctc gagtacagc tgggactgt ctgatcaaa tcaaacgac gaaacgaaa caagaaaac gacttaaca aattcgctc aaaaaactt ctatctttg gcaaaaata caaagcgaa aagcctgtg atgaagtgc acctgcggc cacgtttgc ggcaagccc agcacgcaa cagttcaaa aagccggtg gcccccaaa gtcgtgaaa caaaaacaa gtgagccat gagaccaat cacgttcaa gagaaagtg gaggtgtct gaacccaaa ccggcgccc gaaccgaag aaagcgccc ccggaggag cccttccat gatacgagc agttcggtg gttcacttg ttcagccaa cgcgataag ttcaggata aaggggcgc acccgcttc aagcacttg gtggtcgaa cgtaaacta gttcctaat aaatccgag aacaagcac gagatcgtg tccatggat attgtgtct caggacaag cctgacgat atgcctctt gtcaattct agcgggctt ccgtcgatg ttaatgggt gataacgac acgcgtatg agtgagaag agcttcagt ggttttttg ggtgataat cagtcgaat tcggccatg tcgaccgga ttgtcgaaa atactgaaa gaaaatgag aaccaatgg atcaatgcc gacgttgcc gactattcg ctcagcagt cttttgggc catttggac tgtccgtcg aaaacgaac caatcaatg gtcagtctt tcgcacgac gacacgcaa ttgtcccaa gatgtggat gcccagtta cggactctg ttgattgaa acgagtgtc gactacacg gccaaattc gctgatctg gcagctcaa gttgtggaa aataagaag taa
- >TC007439 RA
cagagaaaa cagttatgt gtactttgt tgagtgtat aatggacca attttttgc tttattttt tcccagact ttgaatttg taacaatga ccgacaacc atgaggtcc ccaacccta aagacttcc tggcccaga tgggcccca tcgagaccc ttctgggtt cgctgtctt acaacgaca tcgacaatg tcgatggca aaacccagc aattgcgca ccagcgctc gcgttttca aaaaaatga aattcgacc tctcctccg tcgagaaaa aagtcgaaa agaaggagg aagtcaagc cggaggaga agcaagaag tgaagaaac cggccaatc cgttctggt cgagcgaag agaagaatc ttttcttcg aagcgttga acgaatacg ggaaggatt ttgaaagca tccacgggt acatcagca cccgcttga agaagaaag gggtcccgg aggacctca tcaagaaca agctgcaag tcaggcact tttattaca aaaccttcc acatgttgt tggggccgt tgaaattta acgagaaaa ttccgaaaa acgtgcaag agctgtaca cgttgatca attttggcg aaatgagga gaaaacttg gcagtatct cgaaaaaaa attgctcga aactgaacg atttgattg caaacggtt cagttcctt tgaggctta aggggaaaa ccgtcaggg ttaaaactc caatgtgta gggctttga gacgcttga accaattag atgaaaaat acgatgatc ttaaattgc caacgaggg ttttggtcg agctcagac caaaaaaca tggcttcgt ttctcagag tgcaaagtg ttgctcaaa atccgcgaa tcaaagtcg cactctcgc tacaaaagc ggttgagct cgcttatca cgtgcataa acacccgct ggaagacct ccgaggccg ttgtttacg ataaagctg tgatttcga caaacccgg taacaaaaa attgcgttc ctgatacga aaacaattg atgatagta cgttgttac tgaatccgc tcttaaggc tgtctcctc cgccggagg cccacattg agttgcctt caatcgata tcagcgaat atttcaacg gtcaaagca tatcactta atgcattcg aattgagaa aaggggtta aaaaggagg agcctgaag cgcccccca agactgaaa aatcgccga acaagcctg acgaagagg aaatcccca aatcggtca ccagcgtcg tggacgagg cagtcaaca caattctct cgctgcaag tccagaata aaaccgaca ccaagactg ccaccacaa ccaacccta aagatatcc ccaatgagg agaaaatat caaggatcg agcaaatca agcaggggt ggactttgg agaattgcg ccagtttga ccattggcg agatctact tgatgtacg ggtgtgact caaaaatgg tgctcgagt acagctggg actgtctga tcaaatcaa acgacgaaa cgaaacaag aaaacgact taacaaatt cgctcaaaa aacttctat ctttggcaa aaatacaaa gcgaaaagc ctgtgatga agtgcacct gcggccacg tttgcggca agcccagca cgcaacagt tcaaaaagc cggtggccc ccaaagtcg tgaaacaaa aacaagtga gccatgaga ccaatcacg ttcaagaga aagtggagg tgtctgaac ccaaaccgg cgcccgaac cgaagaaag cgcccccgg aggagccct tccatgata cgagcagtt cggtggttc acttgttca gccaacgcg ataagttca ggataaagg ggcgcaccc gcttcaagc acttggtgg tcgaacgta aactagttc ctaataaat ccgagaaca agcacgaga tcgtgtcca tggatattg tgtctcagg acaagcctg acgatatgc ctcttgtca attctagcg ggcttccgt cgatgttaa tgggtgata acgacacgc gtatgagtg agaagagct tcagtggtt ttttgggtg ataatcagt cgaattcgg ccatgtcga ccggattgt cgaaaatac tgaaagaaa atgagaacc aatggatca atgccgacg ttgccgact attcgctca gcagtcttt tgggccatt tggactgtc cgtcgaaaa cgaaccaat caatggtca gtctttcgc acgacgaca cgcaattgt cccaagatg tggatgccc agttacgga ctctgttga ttgaaacga gtgtcgact acacggcca aattcgctg atctggcag ctcaagttg tggaaaata agaagtaaa atagaccaa ttggcacaa acttaatct ttgtacttt tatgtttgt taataattt atgtatagc gaaagatat tttagcgcg aaaaattta tatttcata aggttgtat tatattgca ataaattta acatttgtg a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
