Status
Please wait ...
Gene information
General information
TC007439 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4487263..4492410
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007439
    iBeetle screen iB_01189

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007439 RA
      MTDNHEVPN PKDFLAQMG PIETLLGSL SYNDIDNVD 
      GKTQQLRTS ARVFKKMKF DLSSVEKKV EKKEEVKPE 
      EKQEVKKPA NPFWSSEEK NLFFEALNE YGKDFESIH 
      GYISTRLKK KGVPEDLIK NKLQVRHFY YKTFHMLLG 
      PLKFNEKIP KNVQELYTL INFGEMRRK LGSISKKNC 
      SKLNDLIAN GSVPLRLKG KTVRVKTPM CRALRRLNQ 
      LDEKYDDLK LPTRVLVEL RPKNMASFL RVQSVAQNP 
      RIKVALSLQ KRLSSLITC INTRWKTSE AVVYDKAVI 
      STNPVTKNC VPDTKTIDD STLLLNPLL RLSPPPEAH 
      IELPSIDIS EYFNGQSIS LNAFELRKG VKKEEPEAP 
      PKTEKSPNK PDEEEIPKS VTSVVDEAV NTILSLQVQ 
      NKTDTKTAT TTNPKDIPN EEKISRIEQ IKQGWTLEN 
      CASLTIGEI YLMYGCDSK MVLEYSWDC LIKSNDETK 
      QENDLTNSL KKLLSLAKI QSEKPVMKC TCGHVCGKP 
      STQQFKKPV APKVVKQKQ VSHETNHVQ EKVEVSEPK 
      PAPEPKKAP PEEPFHDTS SSVVHLFSQ RDKFRIKGR 
      TRFKHLVVE RKLVPNKSE NKHEIVSMD IVSQDKPDD 
      MPLVNSSGL PSMLMGDND TRMSEKSFS GFLGDNQSN 
      SAMSTGLSK ILKENENQW INADVADYS LSSLLGHLD 
      CPSKTNQSM VSLSHDDTQ LSQDVDAQL RTLLIETSV 
      DYTAKFADL AAQVVENKK
    CDS sequence
    • >TC007439 RA
      atgaccgac aaccatgag gtccccaac cctaaagac 
      ttcctggcc cagatgggc cccatcgag acccttctg 
      ggttcgctg tcttacaac gacatcgac aatgtcgat 
      ggcaaaacc cagcaattg cgcaccagc gctcgcgtt 
      ttcaaaaaa atgaaattc gacctctcc tccgtcgag 
      aaaaaagtc gaaaagaag gaggaagtc aagccggag 
      gagaagcaa gaagtgaag aaaccggcc aatccgttc 
      tggtcgagc gaagagaag aatcttttc ttcgaagcg 
      ttgaacgaa tacgggaag gattttgaa agcatccac 
      gggtacatc agcacccgc ttgaagaag aaaggggtc 
      ccggaggac ctcatcaag aacaagctg caagtcagg 
      cacttttat tacaaaacc ttccacatg ttgttgggg 
      ccgttgaaa tttaacgag aaaattccg aaaaacgtg 
      caagagctg tacacgttg atcaatttt ggcgaaatg 
      aggagaaaa cttggcagt atctcgaaa aaaaattgc 
      tcgaaactg aacgatttg attgcaaac ggttcagtt 
      cctttgagg cttaagggg aaaaccgtc agggttaaa 
      actccaatg tgtagggct ttgagacgc ttgaaccaa 
      ttagatgaa aaatacgat gatcttaaa ttgccaacg 
      agggttttg gtcgagctc agaccaaaa aacatggct 
      tcgtttctc agagtgcaa agtgttgct caaaatccg 
      cgaatcaaa gtcgcactc tcgctacaa aagcggttg 
      agctcgctt atcacgtgc ataaacacc cgctggaag 
      acctccgag gccgttgtt tacgataaa gctgtgatt 
      tcgacaaac ccggtaaca aaaaattgc gttcctgat 
      acgaaaaca attgatgat agtacgttg ttactgaat 
      ccgctctta aggctgtct cctccgccg gaggcccac 
      attgagttg ccttcaatc gatatcagc gaatatttc 
      aacggtcaa agcatatca cttaatgca ttcgaattg 
      agaaaaggg gttaaaaag gaggagcct gaagcgccc 
      cccaagact gaaaaatcg ccgaacaag cctgacgaa 
      gaggaaatc cccaaatcg gtcaccagc gtcgtggac 
      gaggcagtc aacacaatt ctctcgctg caagtccag 
      aataaaacc gacaccaag actgccacc acaaccaac 
      cctaaagat atccccaat gaggagaaa atatcaagg 
      atcgagcaa atcaagcag gggtggact ttggagaat 
      tgcgccagt ttgaccatt ggcgagatc tacttgatg 
      tacgggtgt gactcaaaa atggtgctc gagtacagc 
      tgggactgt ctgatcaaa tcaaacgac gaaacgaaa 
      caagaaaac gacttaaca aattcgctc aaaaaactt 
      ctatctttg gcaaaaata caaagcgaa aagcctgtg 
      atgaagtgc acctgcggc cacgtttgc ggcaagccc 
      agcacgcaa cagttcaaa aagccggtg gcccccaaa 
      gtcgtgaaa caaaaacaa gtgagccat gagaccaat 
      cacgttcaa gagaaagtg gaggtgtct gaacccaaa 
      ccggcgccc gaaccgaag aaagcgccc ccggaggag 
      cccttccat gatacgagc agttcggtg gttcacttg 
      ttcagccaa cgcgataag ttcaggata aaggggcgc 
      acccgcttc aagcacttg gtggtcgaa cgtaaacta 
      gttcctaat aaatccgag aacaagcac gagatcgtg 
      tccatggat attgtgtct caggacaag cctgacgat 
      atgcctctt gtcaattct agcgggctt ccgtcgatg 
      ttaatgggt gataacgac acgcgtatg agtgagaag 
      agcttcagt ggttttttg ggtgataat cagtcgaat 
      tcggccatg tcgaccgga ttgtcgaaa atactgaaa 
      gaaaatgag aaccaatgg atcaatgcc gacgttgcc 
      gactattcg ctcagcagt cttttgggc catttggac 
      tgtccgtcg aaaacgaac caatcaatg gtcagtctt 
      tcgcacgac gacacgcaa ttgtcccaa gatgtggat 
      gcccagtta cggactctg ttgattgaa acgagtgtc 
      gactacacg gccaaattc gctgatctg gcagctcaa 
      gttgtggaa aataagaag taa
    mRNA sequence
    • >TC007439 RA
      cagagaaaa cagttatgt gtactttgt tgagtgtat 
      aatggacca attttttgc tttattttt tcccagact 
      ttgaatttg taacaatga ccgacaacc atgaggtcc 
      ccaacccta aagacttcc tggcccaga tgggcccca 
      tcgagaccc ttctgggtt cgctgtctt acaacgaca 
      tcgacaatg tcgatggca aaacccagc aattgcgca 
      ccagcgctc gcgttttca aaaaaatga aattcgacc 
      tctcctccg tcgagaaaa aagtcgaaa agaaggagg 
      aagtcaagc cggaggaga agcaagaag tgaagaaac 
      cggccaatc cgttctggt cgagcgaag agaagaatc 
      ttttcttcg aagcgttga acgaatacg ggaaggatt 
      ttgaaagca tccacgggt acatcagca cccgcttga 
      agaagaaag gggtcccgg aggacctca tcaagaaca 
      agctgcaag tcaggcact tttattaca aaaccttcc 
      acatgttgt tggggccgt tgaaattta acgagaaaa 
      ttccgaaaa acgtgcaag agctgtaca cgttgatca 
      attttggcg aaatgagga gaaaacttg gcagtatct 
      cgaaaaaaa attgctcga aactgaacg atttgattg 
      caaacggtt cagttcctt tgaggctta aggggaaaa 
      ccgtcaggg ttaaaactc caatgtgta gggctttga 
      gacgcttga accaattag atgaaaaat acgatgatc 
      ttaaattgc caacgaggg ttttggtcg agctcagac 
      caaaaaaca tggcttcgt ttctcagag tgcaaagtg 
      ttgctcaaa atccgcgaa tcaaagtcg cactctcgc 
      tacaaaagc ggttgagct cgcttatca cgtgcataa 
      acacccgct ggaagacct ccgaggccg ttgtttacg 
      ataaagctg tgatttcga caaacccgg taacaaaaa 
      attgcgttc ctgatacga aaacaattg atgatagta 
      cgttgttac tgaatccgc tcttaaggc tgtctcctc 
      cgccggagg cccacattg agttgcctt caatcgata 
      tcagcgaat atttcaacg gtcaaagca tatcactta 
      atgcattcg aattgagaa aaggggtta aaaaggagg 
      agcctgaag cgcccccca agactgaaa aatcgccga 
      acaagcctg acgaagagg aaatcccca aatcggtca 
      ccagcgtcg tggacgagg cagtcaaca caattctct 
      cgctgcaag tccagaata aaaccgaca ccaagactg 
      ccaccacaa ccaacccta aagatatcc ccaatgagg 
      agaaaatat caaggatcg agcaaatca agcaggggt 
      ggactttgg agaattgcg ccagtttga ccattggcg 
      agatctact tgatgtacg ggtgtgact caaaaatgg 
      tgctcgagt acagctggg actgtctga tcaaatcaa 
      acgacgaaa cgaaacaag aaaacgact taacaaatt 
      cgctcaaaa aacttctat ctttggcaa aaatacaaa 
      gcgaaaagc ctgtgatga agtgcacct gcggccacg 
      tttgcggca agcccagca cgcaacagt tcaaaaagc 
      cggtggccc ccaaagtcg tgaaacaaa aacaagtga 
      gccatgaga ccaatcacg ttcaagaga aagtggagg 
      tgtctgaac ccaaaccgg cgcccgaac cgaagaaag 
      cgcccccgg aggagccct tccatgata cgagcagtt 
      cggtggttc acttgttca gccaacgcg ataagttca 
      ggataaagg ggcgcaccc gcttcaagc acttggtgg 
      tcgaacgta aactagttc ctaataaat ccgagaaca 
      agcacgaga tcgtgtcca tggatattg tgtctcagg 
      acaagcctg acgatatgc ctcttgtca attctagcg 
      ggcttccgt cgatgttaa tgggtgata acgacacgc 
      gtatgagtg agaagagct tcagtggtt ttttgggtg 
      ataatcagt cgaattcgg ccatgtcga ccggattgt 
      cgaaaatac tgaaagaaa atgagaacc aatggatca 
      atgccgacg ttgccgact attcgctca gcagtcttt 
      tgggccatt tggactgtc cgtcgaaaa cgaaccaat 
      caatggtca gtctttcgc acgacgaca cgcaattgt 
      cccaagatg tggatgccc agttacgga ctctgttga 
      ttgaaacga gtgtcgact acacggcca aattcgctg 
      atctggcag ctcaagttg tggaaaata agaagtaaa 
      atagaccaa ttggcacaa acttaatct ttgtacttt 
      tatgtttgt taataattt atgtatagc gaaagatat 
      tttagcgcg aaaaattta tatttcata aggttgtat 
      tatattgca ataaattta acatttgtg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY