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Gene information
General information
TC007435 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4508603..4513970
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007435
    iBeetle screen iB_01188

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber complete not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007435 RA
      MVRKKIDNR IRVLIENGV KLGHRTLFA IVGDKGRDQ 
      VVILHHMLA KAEVKARPS VLWCYKKEL GFSSHRKKR 
      MKQLQHKIQ TGKLSVNED DPFELFLSS TKIRYCYYS 
      ETHKILGNT FGMCVLQDF EALTPNLLA RTIETVEGG 
      GLVVILLRS VASLKQLYT MSMDVHQRF RTEAHQDIV 
      CRFNERFLL SLASCKRCL VVDDQLSVL PISSHNLHV 
      TPVEPSQEP LPSESELTD LKNQLRDTQ PVGNLINCC 
      KTLDQGKAL LKFIEAISE KTLRSTVSL TAARGRGKS 
      AALGLSVAA AVAFGYSNI FVTSPSPEN LNTFFEFVF 
      KGFDALEYQ EHIDYSLVQ STNPEFNKA IVRVNIFRD 
      HRQTIQYIQ PTDSQRLSQ AELLVIDEA AAIPLPYVK 
      SMLGPYLVF LASTINGYE GTGRSLSLK LLQQLRIQA 
      VPVGANTNA ANKKDSGAA TGRTLHEVT LDESIRYKP 
      GDDVEAWLT KLLCLDATS VAPILSGCP PPDSCDLYY 
      INRDTLFCY HKASEEFLQ RLVALYVAS HYKNTPNDL 
      QMMSDAPAH HLFCLLGPV DPNRKTLPE VLVIIQVCL 
      EGEISRSSV VEGFSRGKR ASGDLIPWT VGQQYQDPD 
      FPRLSGARI VRIATHPDY QGMGYGAKA LNLLKQYYE 
      FKIPSTDEF EDVVEEISN VREEEVGLL EETIEPRKS 
      LPPLLIKLS DRKPEKLDY LGVSYGLTE QLLKFWKRA 
      GYVPVYLRQ TTNDLTGEH SCIMLNVLN DEDGSNDWL 
      MAYWSDFRR RFVNLLGYQ FSKFSPSLA LGVLTNKTR 
      KITSKSLSR AELEIHLTS YDIKRLEMY SNNLVDYHL 
      IIDLLPTLA KLYFLNQMG DVQMSAVQS AILLGVGLQ 
      YKTVDTLAA ELDLPSSQL LGLFNRMIR RIVQYLNSV 
      LEQDIEKSL APRKDVELT PVAKSMQQE LDEAAQELQ 
      KKQKKELEK LKNESLTQF AIKGSEEEW GKVLTGKSK 
      KNIITIKSG EKRLHESNT DEVKEDPIK KKKNKNKKR 
      KNN
    CDS sequence
    • >TC007435 RA
      atggtgcgt aaaaaaatt gataatagg attcgagtg 
      ttgatcgaa aatggtgtc aagctgggg caccggact 
      ttgtttgcc atagttggt gataaaggc cgggatcag 
      gtggtgatt ctccaccac atgcttgca aaagccgaa 
      gttaaggcc cgcccatct gttctgtgg tgttacaaa 
      aaagagtta ggcttcagt agccaccga aaaaagcgc 
      atgaaacag ctccaacat aaaatacaa acgggcaaa 
      cttagcgtc aatgaggac gaccccttt gaacttttc 
      ctctcctct acgaaaatc cgctactgt tactactcc 
      gaaacccac aaaattttg ggcaacacg ttcggcatg 
      tgcgtcctg caggacttc gaagccttg acccccaac 
      ctcctagcc cggactatt gaaacggta gaagggggc 
      ggcttggtt gttatttta ctgcgctcg gtggcgtct 
      ttaaaacag ttgtacacc atgagcatg gacgtccac 
      cagcgcttc cggacggaa gcccaccaa gacatagtc 
      tgccgcttc aacgaacgt ttcctcctg tctctagcc 
      tcctgcaaa cgctgtctt gtggtcgac gaccaactt 
      tccgtcctc ccaatctcc tcacacaac ctccacgtg 
      accccggtc gagccctcg caagagccc ttgccttcg 
      gaaagcgaa ctaaccgac ctcaaaaac cagctcaga 
      gacacccaa ccagtcggt aatttaatc aactgttgc 
      aaaaccctg gaccagggt aaggcgctt ttgaagttc 
      attgaagcc atttcggaa aagacactt cgctcgacg 
      gtgtctctg actgcggca agggggcgc gggaaatcg 
      gccgcgttg gggctgtcg gtggccgca gccgtggcc 
      ttcggatac tcgaatatt ttcgtaacg agtccgagt 
      ccggagaat ttaaacacg tttttcgaa tttgttttt 
      aaaggattt gacgcgctt gaatatcag gaacatata 
      gactatagt ctagtgcaa agcacgaac cccgagttt 
      aataaggcc atagttcgg gtgaatatt ttcagggac 
      cacaggcaa actattcaa tacattcag ccgactgat 
      agtcaaaga ttgagtcaa gccgaactt ttagtaatt 
      gacgaagca gctgcgatt cctctcccg tatgtgaaa 
      tcaatgcta gggccgtat ttagtgttt ttagcctca 
      actatcaat ggttacgag ggcaccggt cgctcctta 
      tctttaaaa ttattgcaa cagttgcga attcaggcg 
      gttccagtg ggtgccaac accaatgcc gcaaataag 
      aaggactcg ggggctgcc actggtcgc acccttcac 
      gaggtcact ttggacgaa tcaattcgg tacaaaccc 
      ggcgatgat gtggaggcg tggctcact aagctctta 
      tgtctcgac gccacttcc gtggccccc atcttgtca 
      gggtgccct ccgcccgac tcctgcgat ttgtattac 
      ataaaccgc gacaccctt ttttgttat cacaaagca 
      tcagaggag tttttgcaa cgtctggtc gccttatac 
      gtcgcctcg cattataaa aacactccg aatgacctt 
      caaatgatg tcggacgcc cccgcacat catttgttt 
      tgtctcttg ggccccgtt gaccccaac cggaagacc 
      ctcccggag gtgctagtc ataatccag gtgtgcctt 
      gaaggcgaa atatcaaga tcctccgtt gttgagggt 
      ttttcccgg gggaagaga gcctccggg gatttgatc 
      ccgtggacg gtgggccag cagtaccaa gacccggac 
      tttccgcgc ctttccggc gcccggatt gtccggatc 
      gccacgcat ccggactac caggggatg ggttatggg 
      gcgaaagcc ctcaattta ctcaaacaa tactacgag 
      tttaaaatc ccgagtacc gacgaattt gaggatgtg 
      gtggaggag attagtaat gtccgggag gaggaggtc 
      ggccttttg gaggaaaca attgaacca aggaaaagc 
      ctcccccct ctgttaatc aaattgagt gaccgcaaa 
      ccagaaaaa ctggattat ctgggggtt tcctacggt 
      ttaaccgaa cagttgctg aagttttgg aagcgggct 
      gggtacgtc cctgtttat ttgagacaa acgacgaac 
      gatttaaca ggggagcat tcgtgtatt atgttgaac 
      gttcttaac gatgaggat gggagtaac gattggttg 
      atggcgtat tggagcgat ttcaggaga cgtttcgtt 
      aatctttta ggctaccag tttagcaaa ttttcgcca 
      agtctagcc ttgggcgtt ttgacaaat aagacgcga 
      aaaatcacg tctaaaagt ttgtccagg gccgaattg 
      gaaatacat ttgaccagt tacgacata aaacggctg 
      gaaatgtac agtaataat ttagttgat taccattta 
      atcatcgac ttgttgccg actttggca aaactgtat 
      ttcctgaac cagatgggc gatgttcaa atgtcggca 
      gtgcaatca gcaattttg ttgggtgtg gggctccag 
      tacaagaca gttgacacg ctcgcagcc gagctcgat 
      ttgccctca tctcagctt ttgggcctt tttaaccga 
      atgattaga cgaatcgtt cagtattta aattcagtc 
      cttgagcaa gacattgaa aagtctttg gccccccga 
      aaggacgtc gaactgact ccagtcgcg aaatcaatg 
      caacaggaa ctagacgaa gcggcgcaa gaattacaa 
      aaaaagcaa aaaaaggag ttagagaaa ttgaaaaat 
      gagtcttta acccaattc gcgattaag gggtctgag 
      gaagagtgg ggcaaagtc ttgacggga aaaagcaaa 
      aaaaatatt atcacaatt aaaagtggc gagaaacgg 
      ctacatgag agtaatacc gacgaagtg aaagaggac 
      ccaatcaag aagaagaag aataaaaac aagaagcgg 
      aaaaataac tga
    mRNA sequence
    • >TC007435 RA
      cgtcttaaa aaacaaatt tttcagatg aatggagcc 
      aatccggtt gtatcttgt caaaaatta tcagctcaa 
      gtgttctcc cagcttcta ggttcacac ttggaaata 
      caaggtctt gttgccttc cagcaacat ttgcgaatg 
      gagttgacc agttaaagt ctccatcta tcatccgcc 
      ccaaatatt gggcacact cgtgtgctg cgagtgtta 
      ctgcatcag ctttaaagg ccctattcg cttgtttgt 
      ttttgggca aacagctaa atttgtaag gttctgact 
      tgtttttta agacgccaa atgttctgg cccagaagt 
      aacatttta tactctact caactaata attgaaata 
      ttaaaaaat atttttccg gaatgattc ttacttatt 
      aacacttcc ccatctaat gttaagttt ctgctccta 
      aaatctact ccgattgtg gcctccgac tgttccaaa 
      aaagcccct tcacagaat ctataagtc agtgaggtt 
      ttacccaca tttgagtcc ccaatgaca ataatctta 
      aatatcttc ttgtagttt gttgttttg ttaatcgtg 
      ggggcttca ttagcattc gttggtgca gtgttttcc 
      atttttact ccattcaga taattcttt aatcgctat 
      atgataaaa aattacgta gttgaaaca acaataata 
      aaaattgat ttacctcaa atgagtcac ttgattaca 
      tcaaatgta cttttccag gtcattaga aagtataaa 
      tcatgttga ttaattgtt ttttgtgta aaaaatagg 
      gtttaaaaa aatattctg acgtctact tgacaatca 
      gctgcttga cattacagt tacgaaacc caaattccc 
      taaaaaact gctaaccac acttttgtt gtggttgct 
      taacctcaa aaaaacgtg ttaatttga aaaatggtg 
      cgtaaaaaa attgataat aggattcga gtgttgatc 
      gaaaatggt gtcaagctg gggcaccgg actttgttt 
      gccatagtt ggtgataaa ggccgggat caggtggtg 
      attctccac cacatgctt gcaaaagcc gaagttaag 
      gcccgccca tctgttctg tggtgttac aaaaaagag 
      ttaggcttc agtagccac cgaaaaaag cgcatgaaa 
      cagctccaa cataaaata caaacgggc aaacttagc 
      gtcaatgag gacgacccc tttgaactt ttcctctcc 
      tctacgaaa atccgctac tgttactac tccgaaacc 
      cacaaaatt ttgggcaac acgttcggc atgtgcgtc 
      ctgcaggac ttcgaagcc ttgaccccc aacctccta 
      gcccggact attgaaacg gtagaaggg ggcggcttg 
      gttgttatt ttactgcgc tcggtggcg tctttaaaa 
      cagttgtac accatgagc atggacgtc caccagcgc 
      ttccggacg gaagcccac caagacata gtctgccgc 
      ttcaacgaa cgtttcctc ctgtctcta gcctcctgc 
      aaacgctgt cttgtggtc gacgaccaa ctttccgtc 
      ctcccaatc tcctcacac aacctccac gtgaccccg 
      gtcgagccc tcgcaagag cccttgcct tcggaaagc 
      gaactaacc gacctcaaa aaccagctc agagacacc 
      caaccagtc ggtaattta atcaactgt tgcaaaacc 
      ctggaccag ggtaaggcg cttttgaag ttcattgaa 
      gccatttcg gaaaagaca cttcgctcg acggtgtct 
      ctgactgcg gcaaggggg cgcgggaaa tcggccgcg 
      ttggggctg tcggtggcc gcagccgtg gccttcgga 
      tactcgaat attttcgta acgagtccg agtccggag 
      aatttaaac acgtttttc gaatttgtt tttaaagga 
      tttgacgcg cttgaatat caggaacat atagactat 
      agtctagtg caaagcacg aaccccgag tttaataag 
      gccatagtt cgggtgaat attttcagg gaccacagg 
      caaactatt caatacatt cagccgact gatagtcaa 
      agattgagt caagccgaa cttttagta attgacgaa 
      gcagctgcg attcctctc ccgtatgtg aaatcaatg 
      ctagggccg tatttagtg tttttagcc tcaactatc 
      aatggttac gagggcacc ggtcgctcc ttatcttta 
      aaattattg caacagttg cgaattcag gcggttcca 
      gtgggtgcc aacaccaat gccgcaaat aagaaggac 
      tcgggggct gccactggt cgcaccctt cacgaggtc 
      actttggac gaatcaatt cggtacaaa cccggcgat 
      gatgtggag gcgtggctc actaagctc ttatgtctc 
      gacgccact tccgtggcc cccatcttg tcagggtgc 
      cctccgccc gactcctgc gatttgtat tacataaac 
      cgcgacacc cttttttgt tatcacaaa gcatcagag 
      gagtttttg caacgtctg gtcgcctta tacgtcgcc 
      tcgcattat aaaaacact ccgaatgac cttcaaatg 
      atgtcggac gcccccgca catcatttg ttttgtctc 
      ttgggcccc gttgacccc aaccggaag accctcccg 
      gaggtgcta gtcataatc caggtgtgc cttgaaggc 
      gaaatatca agatcctcc gttgttgag ggtttttcc 
      cgggggaag agagcctcc ggggatttg atcccgtgg 
      acggtgggc cagcagtac caagacccg gactttccg 
      cgcctttcc ggcgcccgg attgtccgg atcgccacg 
      catccggac taccagggg atgggttat ggggcgaaa 
      gccctcaat ttactcaaa caatactac gagtttaaa 
      atcccgagt accgacgaa tttgaggat gtggtggag 
      gagattagt aatgtccgg gaggaggag gtcggcctt 
      ttggaggaa acaattgaa ccaaggaaa agcctcccc 
      cctctgtta atcaaattg agtgaccgc aaaccagaa 
      aaactggat tatctgggg gtttcctac ggtttaacc 
      gaacagttg ctgaagttt tggaagcgg gctgggtac 
      gtccctgtt tatttgaga caaacgacg aacgattta 
      acaggggag cattcgtgt attatgttg aacgttctt 
      aacgatgag gatgggagt aacgattgg ttgatggcg 
      tattggagc gatttcagg agacgtttc gttaatctt 
      ttaggctac cagtttagc aaattttcg ccaagtcta 
      gccttgggc gttttgaca aataagacg cgaaaaatc 
      acgtctaaa agtttgtcc agggccgaa ttggaaata 
      catttgacc agttacgac ataaaacgg ctggaaatg 
      tacagtaat aatttagtt gattaccat ttaatcatc 
      gacttgttg ccgactttg gcaaaactg tatttcctg 
      aaccagatg ggcgatgtt caaatgtcg gcagtgcaa 
      tcagcaatt ttgttgggt gtggggctc cagtacaag 
      acagttgac acgctcgca gccgagctc gatttgccc 
      tcatctcag cttttgggc ctttttaac cgaatgatt 
      agacgaatc gttcagtat ttaaattca gtccttgag 
      caagacatt gaaaagtct ttggccccc cgaaaggac 
      gtcgaactg actccagtc gcgaaatca atgcaacag 
      gaactagac gaagcggcg caagaatta caaaaaaag 
      caaaaaaag gagttagag aaattgaaa aatgagtct 
      ttaacccaa ttcgcgatt aaggggtct gaggaagag 
      tggggcaaa gtcttgacg ggaaaaagc aaaaaaaat 
      attatcaca attaaaagt ggcgagaaa cggctacat 
      gagagtaat accgacgaa gtgaaagag gacccaatc 
      aagaagaag aagaataaa aacaagaag cggaaaaat 
      aactgattt tattaaaaa ataaaaatg tgttatact 
      tgtgtaact gaatacact tcgttatct aatgtatcg 
      tacaagaca aaataaacg ttgtaacac gagagaaac 
      agtccgttt tgtgttact ttcacaatt cctttgtaa 
      gtccctgat aaaagcgac gttggcagc actgaaacg 
      tcaagtgac ggcaaaatc gtgtaattc cccttgaca 
      atcgggcat ctacatggc acactattc tttatgaaa 
      acttccggg caagtttgg ccaaccgga aatccgtaa 
      tcacacaaa ttgttataa gtacaggac cctaaacca 
      ttctcacaa gggattggt atccacacc ccccaaata 
      tcacgttct atccataag gacgcctaa aataatgtg 
      tcaaagatg gacgcctaa ctgggggaa gtaccttaa 
      tcgggccta caaggtcaa ttgtggaac tcaggctcc 
      cagtgacct taattttgg attttgaac ccatttcaa 
      gtgtgacat tagctacgg tcacagggt aatctctac 
      ttgaacatt ttgaaattt tcaagtctt ttatcagca 
      tggaattct aaaaaatca tttattgat ttatgactg 
      cagataaat acgtacata actcgtttc tgtccttat 
      taaaaatgg ttgtagcca taataatac aaagatcaa 
      gtttaaatg gcccataat tatcacaaa attcacaga 
      actgcgcaa attaatgca ctggaaaaa attaactcg 
      tcggtctta agaagtagt cat
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