Status
Please wait ...
Gene information
General information
TC007434 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4514033..4520630
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007434
    iBeetle screen iB_01187

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 62.5% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    dorsal thoracic muscles partially not present - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007434 RA
      MEIDEEVYA DGASASGTE LVQEWLNLF ESNSLTTNQ 
      FKQHWKTWV PKLYAPPVN ESCLNWRFD EDLAQKLLF 
      NVLEEFICK GDPSLVLAR LNEMDKPPS VCGRVFKLG 
      EPTYSCREC GMDNTCVLC VNCFKNSEH RFHKYKMGT 
      SQGGGCCDC GDVEAWKKA PFCDVHIAG TRNDAHRSL 
      PEDLKQRTR IVFEAVLWY AYCLLRTGF VSDVRFGDI 
      TDTFFDSDA YCTILYNDE IHTFEQVIS TLTRVLKCN 
      QRTAIEFVT NIDREGRAV VKCSSFQHC TELKQEIEK 
      YTSRHGNKP LKVLVSHAY VIAHQIYAS KLLTWLQNF 
      LGHGEGFRS IFAEVVLET PPNETCIIQ GILLKDSSL 
      WKSARSQWQ RLLISGMLL EYENKKELA KIFTKNYGP 
      VMKDFIKDD HDHSYSISS LSVQLFTVP TLAHHLIAC 
      DDVLYILLN TFMSECSTK CNKAGKLEF ERSPSSHAF 
      KRAHFMLYD LRYLLSSIP ENWSDELRK NFLHGLFIM 
      MNLFTKMQG MDAVTRQVG QHMEYEPEW ESAFHLHIR 
      LAYCISLAI KWCSTDRVV LIKAYRAVL KKLDENPCY 
      DPHDRWEAI ELVDHSASC IVYDVASRP VSIHLPLSR 
      FLAGLHLYL EKFDIHFNE MQCPKPTPV EIMEPVLRC 
      QVMIAQVHA GMWRRNGYA LVNQLYFYH NVKCRTEML 
      DRDIALLQM CASLIESNE FLIHVLHKF NLVNWALSN 
      YEITSLKAP EEDSMRQTI SLVEEFLQL LIVIIGERY 
      MPGISNVST EDRIKKEII QHLCIKPMP HSELNKIIT 
      DDIAHETAL DDVIDELAV FKKPTQTSG KGVYELKEQ 
      YYDEYNVFF YHYTREELS KSEEAQRKR RKTATDLEC 
      CPPPRLLKL NESFHMLVN LLQCDVMLH IMQIVLERC 
      NNLRARSFS ELQLHKVLH LIGYALQEE ESKHYPFFK 
      FIHNSAKFN IFNLLTDLL ECPRVDAHK DLLKWVLRK 
      YKEVANQKE QAEGSAQTN AEASTSGDI DSEKERRAK 
      LAAERRAKV MAQITAMQK TFMKENKEL FEESETSTR 
      FEESAMEVT ESGEEQPIA LGPKQTTRL GIEKTFVCI 
      LCQEEERVT KDGPTLVLA AFVQQSTVL CQYKNNEAL 
      TNMQDPLYL NSNMGPAPH TSTCGHVMH SECWRKYFD 
      NVMVREHRR PYRLRHPAS FDVEKQEFL CPLCECLSN 
      TVLPLIPPL SVLQPSYPK SGTTYEDYL ACIDVILRK 
      KVKVCHGMF KCNTTDCAN EHCTACLNG SNSTNLESE 
      SLAICEANC LLQPYQVFY SCIFDRVED FGVLQPKFE 
      ELFPENCPI LDTNLKDMV LLFAQVTYT RGLNVNPHP 
      SDKRLAPMA WKSLSYTTH AIEVLLRDS NKPLLGNLS 
      SRQRDCIET LVRIIGVLG TTWPNSVVI NNHALSLLA 
      ILHENGNEG PSILQWDSF GFLVPLTFA LPSLAGKEN 
      PTPIPTGGN LELYTLRTV FISHIVKIL VLMDVEKLA 
      DCMDTGTSD EDDILKVLQ IMGKSHDRI SGHVVWKHV 
      QEASIPFLR CCVLFYHYL TDVPAPSVL LEVGGDTFA 
      NMCTYLDLP QTPQTLFNS DKVLRLVSK WCQHEEVVS 
      FLQGTPLQV IHEPLSVPK LIDLPTDYS DLINTVSTF 
      TCPNSDEDA RTPSMCLVC GEILCSQSY CCQMELHNM 
      HVGACNYHA QMCGAGVGI FLRVRECEI LFLATPHRG 
      SFVSPPYLD DYGETDMGL RRGNPLRLC QERYRKLQT 
      LWLSHSIHE EIARAIESN SHVITTQWH HL
    CDS sequence
    • >TC007434 RA
      atggaaata gacgaggaa gtctacgcg gacggcgcg 
      tccgcttcg ggtaccgaa ctcgtgcaa gagtggctc 
      aaccttttc gagtcaaac agcctcacc accaaccaa 
      ttcaagcag cactggaag acatgggtg ccgaaactg 
      tacgccccc ccggtgaac gagtcgtgc ctcaattgg 
      cggttcgac gaagacctg gcccagaag ctcctcttc 
      aacgtccta gaggagttt atttgcaag ggggacccg 
      agcctggtc ctggctcgt ttaaacgag atggacaag 
      cccccttcc gtgtgtggg cgggtgttc aagctgggc 
      gaaccgacg tacagctgc cgcgaatgc gggatggac 
      aacacgtgt gtcctttgc gtcaactgt ttcaaaaac 
      tcggagcac cgcttccac aagtataaa atggggacg 
      tcgcaaggc gggggttgt tgcgactgc ggggacgtc 
      gaggcctgg aagaaggcc ccgttttgc gatgtgcac 
      atcgccggg acgcgcaac gacgcccac aggagcctc 
      cctgaggat ttgaaacag aggacgcgc attgtcttc 
      gaggcggtc ttgtggtac gcttattgt ctcctcagg 
      acggggttc gtctccgac gttcgcttc ggggacatc 
      accgacacg ttcttcgac agtgacgcc tactgtacc 
      atcctctac aacgacgaa atccatact tttgaacaa 
      gtcatttcg accttgacc cgcgttttg aaatgcaat 
      caaaggact gcgattgag ttcgtcacg aatatagac 
      cgagagggg cgggctgtt gtcaagtgt tcctcgttc 
      caacactgc acggaactg aagcaagaa atcgagaaa 
      tatacttcg agacatggc aataagccg ctgaaagtg 
      ctagtgagc catgcctac gtcatagcg caccagatc 
      tacgcttcg aagttgttg acttggcta cagaatttt 
      ctgggacat ggcgaaggt tttcgctcg atttttgcc 
      gaagtcgtc ctggaaaca ccgccaaat gagacttgc 
      attatccaa gggatactc ctgaaggac tcctcgttg 
      tggaagagc gcacggtca caatggcaa cgcctctta 
      atctcggga atgctcctc gaatacgag aacaagaaa 
      gagttggcg aaaatcttc accaagaac tacggcccg 
      gtcatgaag gacttcatc aaagatgac catgatcac 
      tcctactcg atttcctcc ctttcggtt cagttgttc 
      acagtgccg accctagcc caccatttg atcgcctgt 
      gatgacgtc ctgtacata ctcctgaat actttcatg 
      tcagagtgt tcgaccaag tgtaacaaa gcgggaaag 
      ctcgaattc gagcggagt ccctcgagt catgccttc 
      aaacgcgcc catttcatg ctgtacgac ctgcggtac 
      ctcctcagc tcgatcccg gagaactgg tccgatgaa 
      ctccgcaaa aacttcctc cacggtctt ttcatcatg 
      atgaacctc tttacgaaa atgcaaggc atggacgcg 
      gtcactcgt caagttggc caacacatg gagtacgaa 
      cccgagtgg gaatcggcc ttccacctc cacatccga 
      ctagcttat tgcatctcc ttggccatc aagtggtgc 
      agcaccgat cgagtggtc ctaatcaag gcctaccga 
      gctgtgttg aaaaaactc gacgaaaac ccatgctac 
      gacccacat gaccgctgg gaagccatc gagttggtc 
      gatcacagc gcctcctgc atcgtgtat gatgtggct 
      tcaagaccg gtctccatc catctaccc ttatcgagg 
      ttcctagca gggttacat ctctacttg gagaagttt 
      gacattcat ttcaatgag atgcagtgt ccgaagcca 
      actcctgtt gagataatg gagccggtt ttgaggtgt 
      caggtcatg atagcccaa gttcatgcc ggcatgtgg 
      cgtcgcaac ggttacgcc ttagtcaac caattgtac 
      ttctaccac aatgttaaa tgccgcacc gaaatgcta 
      gaccgcgat atagcgctc ttacaaatg tgcgcatcg 
      ttgattgaa agcaacgag tttctgatc cacgttttg 
      cacaaattt aatttggtt aattgggcc ttgagtaat 
      tacgaaatt acgagcctt aaggcaccg gaagaggat 
      agtatgagg caaacgatc agtctagtc gaggagttt 
      ttgcagttg ttgattgta atcataggg gagcggtat 
      atgccgggg attagtaac gttagtacc gaagatagg 
      attaaaaaa gagattata caacacttg tgcatcaaa 
      ccgatgccg cactccgag ttgaataag ataataacg 
      gatgatata gcacacgag actgcactt gacgatgtc 
      attgacgag ttggctgtg tttaaaaaa cccacccaa 
      acctccggc aagggtgtc tacgagttg aaggagcaa 
      tactacgat gaatacaac gtcttcttc taccattac 
      acccgagag gaattatcg aaatcggag gaagcgcaa 
      cgaaagaga cgtaaaacc gccacggac cttgaatgt 
      tgcccccct cccagatta ctaaaactg aacgaatcg 
      tttcacatg cttgtgaac ctcctccag tgcgatgtc 
      atgctccac atcatgcag atagtcctc gaacggtgt 
      aacaatcta cgcgcccgc agcttctca gagttacaa 
      ttacacaaa gtgctacac cttatcggt tacgcttta 
      caagaggaa gaatcaaaa cattatccc ttcttcaaa 
      tttatccac aattcggcc aaattcaac attttcaat 
      ctactgacc gacttgtta gaatgcccg cgagtcgac 
      gcccataag gatctcctt aagtgggta ttgcgcaaa 
      tacaaggaa gtagcaaat cagaaggaa caagccgaa 
      ggctcggcc caaaccaac gccgaagcc tcaacttcg 
      ggcgatatt gacagcgag aaggaacga agggcgaaa 
      ctggccgct gagagacgt gccaaagtc atggcacaa 
      atcaccgcc atgcagaag actttcatg aaggagaac 
      aaggagttg tttgaggag agtgaaact agtacgagg 
      tttgaagag tcggctatg gaggttaca gagagtggg 
      gaagaacaa ccgattgcg ttgggtccg aaacaaacc 
      acgcgactc ggtattgag aagacgttc gtttgtatt 
      ttgtgtcag gaggaggag agggtgacc aaagacggt 
      ccgacgctc gtattggcc gctttcgtg caacagtcg 
      actgtttta tgccaatac aaaaacaac gaagcgctg 
      actaatatg caagaccct ttgtatctc aattcgaac 
      atggggccg gccccacat accagtact tgtggccat 
      gtgatgcat agcgagtgt tggcggaag tacttcgat 
      aatgtgatg gtgagggaa caccgaagg ccgtatagg 
      ttgagacac ccggccagt ttcgatgtc gagaaacaa 
      gagtttctc tgcccgctt tgcgagtgt ttgagtaat 
      actgtgtta ccgctcatt ccccctttg agtgtccta 
      caaccgagc tatccgaag agcggtacc acttacgag 
      gattatttg gcgtgtatt gatgtcata ctgaggaag 
      aaggtcaaa gtttgccac ggaatgttc aagtgtaac 
      actaccgac tgcgccaat gagcactgc accgcttgt 
      ttaaacggc tcaaatagc acgaattta gaatccgag 
      tctcttgca atttgtgag gcgaattgt ttgctgcaa 
      ccctaccaa gttttctac tcgtgtatc ttcgaccga 
      gttgaagac ttcggggtg ttgcaaccg aaattcgag 
      gagctgttc cctgagaat tgtccaatc cttgatacg 
      aatttgaaa gatatggtt ctattattt gctcaagtc 
      acttatact agaggctta aatgttaat ccgcaccct 
      tctgataaa agattggcc ccaatggct tggaagtca 
      ctctcgtac accactcac gcaattgaa gtcttactc 
      cgagactcc aacaaacca ctgttaggg aatttgtca 
      agtcggcaa agggactgc atagagacc cttgtccgc 
      attatcgga gttctgggc actacgtgg cccaacagt 
      gtcgttatt aataatcac gctttgagt ttgttggcc 
      attttgcac gagaacggt aacgaagga ccgtcgatc 
      ctccagtgg gattcgttc gggttcttg gtaccgtta 
      acgttcgcg ttaccgagc cttgccggt aaggaaaat 
      cccactcca ataccgacc ggtggtaac ttggagttg 
      tacactctt cgaactgtt ttcatttcc cacatcgtc 
      aaaattctg gttttgatg gatgtagaa aagttggcc 
      gattgtatg gacaccggt acgagcgac gaggatgat 
      attttaaaa gtcttgcaa attatgggc aagtcgcat 
      gaccggata agtggccac gttgtctgg aagcacgtc 
      caggaagct tcaatccca tttttgcgt tgttgtgtc 
      cttttctat cactacttg accgacgta cctgccccg 
      tcagtatta ctcgaagta ggtggcgac actttcgct 
      aacatgtgt acatatttg gacctcccc caaaccccc 
      caaactcta ttcaactcg gacaaagtc ctcagactg 
      gtgtcaaag tggtgccaa catgaggaa gtcgtttcg 
      ttcttacaa ggaactccc ctgcaagtc atccacgaa 
      cctctgtcg gtaccgaaa ctgatcgat ctaccgacc 
      gattatagc gatttgatt aataccgtt tcgacgttc 
      acgtgtccg aatagcgac gaagacgcg cgaactccc 
      agcatgtgt ttggtctgt ggggagatt ttatgttcg 
      cagagttac tgttgtcaa atggagttg cacaacatg 
      catgtgggg gcgtgcaat taccacgca cagatgtgc 
      ggggccggc gttgggatt tttctgaga gtgagagaa 
      tgtgagatt ttgtttttg gcgacgccg catagagga 
      agcttcgtt tcgccgccg tatttggat gattacggc 
      gagacggat atggggttg aggaggggg aatcccctg 
      aggttgtgt caggagagg tacaggaaa ctgcaaaca 
      ctgtggttg agccatagt atacatgag gaaatcgcc 
      agggcgatc gagtcgaat agtcatgtg atcacgacg 
      cagtggcat catctttag
    mRNA sequence
    • >TC007434 RA
      cgccacttc cagcacttt gtccatcca acaatgatt 
      ttattattg tgttagtca atttatgtg caaactagt 
      gcaaaaact aaatttctt attgtttaa tagttactg 
      tgcccctta atatttaat ttatacagt tcaagcaag 
      caattaata atctgtgtt ttgtttgtc attgcaaat 
      tggcatttg cccagatca ccgaagata cggaagttt 
      gcgggtcag aacggttta agtttcttt attaaaagt 
      caaaaatat tcccgaaaa acccaccac tagtatcgt 
      tctcaaaat ccaaatcat cctgtcgaa aaatccgcc 
      gttcaaggg cttaacctt gatccatct gcaggtaat 
      tgcgtttct ttgtgttaa taataatca tctatttta 
      aattcaata gtaaagttc cgacgattc actttcacg 
      tgaaagtcg tccattccg gtcgagttc cgaaaatcg 
      cctttcata atcgccagc acctggcat gacatcatg 
      gaaatagac gaggaagtc tacgcggac ggcgcgtcc 
      gcttcgggt accgaactc gtgcaagag tggctcaac 
      cttttcgag tcaaacagc ctcaccacc aaccaattc 
      aagcagcac tggaagaca tgggtgccg aaactgtac 
      gcccccccg gtgaacgag tcgtgcctc aattggcgg 
      ttcgacgaa gacctggcc cagaagctc ctcttcaac 
      gtcctagag gagtttatt tgcaagggg gacccgagc 
      ctggtcctg gctcgttta aacgagatg gacaagccc 
      ccttccgtg tgtgggcgg gtgttcaag ctgggcgaa 
      ccgacgtac agctgccgc gaatgcggg atggacaac 
      acgtgtgtc ctttgcgtc aactgtttc aaaaactcg 
      gagcaccgc ttccacaag tataaaatg gggacgtcg 
      caaggcggg ggttgttgc gactgcggg gacgtcgag 
      gcctggaag aaggccccg ttttgcgat gtgcacatc 
      gccgggacg cgcaacgac gcccacagg agcctccct 
      gaggatttg aaacagagg acgcgcatt gtcttcgag 
      gcggtcttg tggtacgct tattgtctc ctcaggacg 
      gggttcgtc tccgacgtt cgcttcggg gacatcacc 
      gacacgttc ttcgacagt gacgcctac tgtaccatc 
      ctctacaac gacgaaatc catactttt gaacaagtc 
      atttcgacc ttgacccgc gttttgaaa tgcaatcaa 
      aggactgcg attgagttc gtcacgaat atagaccga 
      gaggggcgg gctgttgtc aagtgttcc tcgttccaa 
      cactgcacg gaactgaag caagaaatc gagaaatat 
      acttcgaga catggcaat aagccgctg aaagtgcta 
      gtgagccat gcctacgtc atagcgcac cagatctac 
      gcttcgaag ttgttgact tggctacag aattttctg 
      ggacatggc gaaggtttt cgctcgatt tttgccgaa 
      gtcgtcctg gaaacaccg ccaaatgag acttgcatt 
      atccaaggg atactcctg aaggactcc tcgttgtgg 
      aagagcgca cggtcacaa tggcaacgc ctcttaatc 
      tcgggaatg ctcctcgaa tacgagaac aagaaagag 
      ttggcgaaa atcttcacc aagaactac ggcccggtc 
      atgaaggac ttcatcaaa gatgaccat gatcactcc 
      tactcgatt tcctccctt tcggttcag ttgttcaca 
      gtgccgacc ctagcccac catttgatc gcctgtgat 
      gacgtcctg tacatactc ctgaatact ttcatgtca 
      gagtgttcg accaagtgt aacaaagcg ggaaagctc 
      gaattcgag cggagtccc tcgagtcat gccttcaaa 
      cgcgcccat ttcatgctg tacgacctg cggtacctc 
      ctcagctcg atcccggag aactggtcc gatgaactc 
      cgcaaaaac ttcctccac ggtcttttc atcatgatg 
      aacctcttt acgaaaatg caaggcatg gacgcggtc 
      actcgtcaa gttggccaa cacatggag tacgaaccc 
      gagtgggaa tcggccttc cacctccac atccgacta 
      gcttattgc atctccttg gccatcaag tggtgcagc 
      accgatcga gtggtccta atcaaggcc taccgagct 
      gtgttgaaa aaactcgac gaaaaccca tgctacgac 
      ccacatgac cgctgggaa gccatcgag ttggtcgat 
      cacagcgcc tcctgcatc gtgtatgat gtggcttca 
      agaccggtc tccatccat ctaccctta tcgaggttc 
      ctagcaggg ttacatctc tacttggag aagtttgac 
      attcatttc aatgagatg cagtgtccg aagccaact 
      cctgttgag ataatggag ccggttttg aggtgtcag 
      gtcatgata gcccaagtt catgccggc atgtggcgt 
      cgcaacggt tacgcctta gtcaaccaa ttgtacttc 
      taccacaat gttaaatgc cgcaccgaa atgctagac 
      cgcgatata gcgctctta caaatgtgc gcatcgttg 
      attgaaagc aacgagttt ctgatccac gttttgcac 
      aaatttaat ttggttaat tgggccttg agtaattac 
      gaaattacg agccttaag gcaccggaa gaggatagt 
      atgaggcaa acgatcagt ctagtcgag gagtttttg 
      cagttgttg attgtaatc ataggggag cggtatatg 
      ccggggatt agtaacgtt agtaccgaa gataggatt 
      aaaaaagag attatacaa cacttgtgc atcaaaccg 
      atgccgcac tccgagttg aataagata ataacggat 
      gatatagca cacgagact gcacttgac gatgtcatt 
      gacgagttg gctgtgttt aaaaaaccc acccaaacc 
      tccggcaag ggtgtctac gagttgaag gagcaatac 
      tacgatgaa tacaacgtc ttcttctac cattacacc 
      cgagaggaa ttatcgaaa tcggaggaa gcgcaacga 
      aagagacgt aaaaccgcc acggacctt gaatgttgc 
      ccccctccc agattacta aaactgaac gaatcgttt 
      cacatgctt gtgaacctc ctccagtgc gatgtcatg 
      ctccacatc atgcagata gtcctcgaa cggtgtaac 
      aatctacgc gcccgcagc ttctcagag ttacaatta 
      cacaaagtg ctacacctt atcggttac gctttacaa 
      gaggaagaa tcaaaacat tatcccttc ttcaaattt 
      atccacaat tcggccaaa ttcaacatt ttcaatcta 
      ctgaccgac ttgttagaa tgcccgcga gtcgacgcc 
      cataaggat ctccttaag tgggtattg cgcaaatac 
      aaggaagta gcaaatcag aaggaacaa gccgaaggc 
      tcggcccaa accaacgcc gaagcctca acttcgggc 
      gatattgac agcgagaag gaacgaagg gcgaaactg 
      gccgctgag agacgtgcc aaagtcatg gcacaaatc 
      accgccatg cagaagact ttcatgaag gagaacaag 
      gagttgttt gaggagagt gaaactagt acgaggttt 
      gaagagtcg gctatggag gttacagag agtggggaa 
      gaacaaccg attgcgttg ggtccgaaa caaaccacg 
      cgactcggt attgagaag acgttcgtt tgtattttg 
      tgtcaggag gaggagagg gtgaccaaa gacggtccg 
      acgctcgta ttggccgct ttcgtgcaa cagtcgact 
      gttttatgc caatacaaa aacaacgaa gcgctgact 
      aatatgcaa gaccctttg tatctcaat tcgaacatg 
      gggccggcc ccacatacc agtacttgt ggccatgtg 
      atgcatagc gagtgttgg cggaagtac ttcgataat 
      gtgatggtg agggaacac cgaaggccg tataggttg 
      agacacccg gccagtttc gatgtcgag aaacaagag 
      tttctctgc ccgctttgc gagtgtttg agtaatact 
      gtgttaccg ctcattccc cctttgagt gtcctacaa 
      ccgagctat ccgaagagc ggtaccact tacgaggat 
      tatttggcg tgtattgat gtcatactg aggaagaag 
      gtcaaagtt tgccacgga atgttcaag tgtaacact 
      accgactgc gccaatgag cactgcacc gcttgttta 
      aacggctca aatagcacg aatttagaa tccgagtct 
      cttgcaatt tgtgaggcg aattgtttg ctgcaaccc 
      taccaagtt ttctactcg tgtatcttc gaccgagtt 
      gaagacttc ggggtgttg caaccgaaa ttcgaggag 
      ctgttccct gagaattgt ccaatcctt gatacgaat 
      ttgaaagat atggttcta ttatttgct caagtcact 
      tatactaga ggcttaaat gttaatccg cacccttct 
      gataaaaga ttggcccca atggcttgg aagtcactc 
      tcgtacacc actcacgca attgaagtc ttactccga 
      gactccaac aaaccactg ttagggaat ttgtcaagt 
      cggcaaagg gactgcata gagaccctt gtccgcatt 
      atcggagtt ctgggcact acgtggccc aacagtgtc 
      gttattaat aatcacgct ttgagtttg ttggccatt 
      ttgcacgag aacggtaac gaaggaccg tcgatcctc 
      cagtgggat tcgttcggg ttcttggta ccgttaacg 
      ttcgcgtta ccgagcctt gccggtaag gaaaatccc 
      actccaata ccgaccggt ggtaacttg gagttgtac 
      actcttcga actgttttc atttcccac atcgtcaaa 
      attctggtt ttgatggat gtagaaaag ttggccgat 
      tgtatggac accggtacg agcgacgag gatgatatt 
      ttaaaagtc ttgcaaatt atgggcaag tcgcatgac 
      cggataagt ggccacgtt gtctggaag cacgtccag 
      gaagcttca atcccattt ttgcgttgt tgtgtcctt 
      ttctatcac tacttgacc gacgtacct gccccgtca 
      gtattactc gaagtaggt ggcgacact ttcgctaac 
      atgtgtaca tatttggac ctcccccaa accccccaa 
      actctattc aactcggac aaagtcctc agactggtg 
      tcaaagtgg tgccaacat gaggaagtc gtttcgttc 
      ttacaagga actcccctg caagtcatc cacgaacct 
      ctgtcggta ccgaaactg atcgatcta ccgaccgat 
      tatagcgat ttgattaat accgtttcg acgttcacg 
      tgtccgaat agcgacgaa gacgcgcga actcccagc 
      atgtgtttg gtctgtggg gagatttta tgttcgcag 
      agttactgt tgtcaaatg gagttgcac aacatgcat 
      gtgggggcg tgcaattac cacgcacag atgtgcggg 
      gccggcgtt gggattttt ctgagagtg agagaatgt 
      gagattttg tttttggcg acgccgcat agaggaagc 
      ttcgtttcg ccgccgtat ttggatgat tacggcgag 
      acggatatg gggttgagg agggggaat cccctgagg 
      ttgtgtcag gagaggtac aggaaactg caaacactg 
      tggttgagc catagtata catgaggaa atcgccagg 
      gcgatcgag tcgaatagt catgtgatc acgacgcag 
      tggcatcat ctttagaga ttatgaaat tgtgatttt 
      tttaaatac taacgatga aatatttgt tgccttttg 
      tctaatttt actaattta ttttgttgg aatttatta 
      cagtactta tggaaaatt acaagctct cttaaggcc 
      cacccaaca cgaaaaaca ctgttgtat aattttagt 
      aggaaattt aatatttat aataggaca gtaaaaaag 
      agttgtata atattatgt gtaatacaa attaattaa 
      aaagataca attttcatt caaaataaa tatattacc 
      cacaggttt ttcttacaa aattattta acagcaccc 
      ccatgattt tttaggctc tgcaacagt atcgattac 
      gatattgtt agtaacagg tagaaaagg cttctgatt 
      ttttagttt gtgagccag agttaaaaa aatcgcttc 
      tatgttatc acattgaga atcgagtct tgcagacgt 
      ttcaaataa ctaaaaaaa atattcttt attagctct 
      agaacggta aggttgcgt ctattaccg gcatgttgt 
      actgatctg caaacctct gcgctacgt ttgtggtaa 
      cggcagcca cgccatcac acttattcc ccactaaaa 
      ttctcggta tgtcgtgga gacaattcc tgttacatt 
      catcaaccc attttttca atgaatcaa aactagcgg 
      cattcgtga cgtcgtaaa ctagaacta tcgcgtgcg 
      tatttctat agtagtgtt tcaccattg acgatctaa 
      atctctctt gacccgcag tgtcccata attgcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY