Status
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Gene information
General information
TC007431 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4611999..4628810
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007431
    iBeetle screen iB_01186

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: entire repetition development delayed, later than schedule, hours 72
    Pupal morphology
    remarks: entire repetition development delayed, later than schedule, hours 72
    Adult morphology
    remarks: entire repetition development delayed, later than schedule, hours 72
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007431 RA
      MGHKAMGAI VALTCVLCY YNSCYCGFV FDDISAIKD 
      NRDLRPHSP LLNIFFNDF WGTPMHKEQ SHKSYRPLC 
      VLTFRWNYL LWQLEPMGY HLVNMLLHS VVCLMYFRM 
      CSMFLPELS SFVAAMLFA VHPIHTEAV TGVVGRAET 
      LSSVFFLAA FMLYAKASK SKKSTGWKY LSFSMLSIA 
      TAMLCKEQG ITVAGVCAA YEIFVTQKV RLPEIKHVV 
      KAAITAKSS YHLPWSSEA TKRLLVLSA TTMCLLLAR 
      LQIMGSQLP VFTRFDNPA SVAPTPARQ LTYHYLISV 
      NLWLLLFPC NLCCDWTMG TIPLVETFA DGRNLATIA 
      AYTFFTALL VTAYTTENR QQRTVILMS LALMALPFM 
      PASNLFFPV GFVIAERVL YMPSMGFCM LVAYGWTVL 
      AEKKGKKLA FFLLSILVV THGTKTYLR NWDWEDEYS 
      IFMSGLRVN QKNAKLFNN VGHALESQG KYEEALKFF 
      KTAVSVQED DVGAHINVG RTYNHLKMF KEAEEAYLR 
      AKSLLPKAK PGESYQARI APNHLNVFL NLANLISKN 
      STRLEEADM LYRQAISMR ADYTQAYIN RGDILIKLN 
      RTKEAQEVY ERALLYDSN NPDIYYNLG VVFLEQGKA 
      SQALAYLDK ALEFDPEHE QALLNSAIL LQEFNRPEL 
      RKIARERLL KLLDKDSNN ERVHFNLGM LAMDEKNIE 
      EAEHWFRRA VHLKADFRS SLFNLALLL ADDQRPLEA 
      APFLNQLVK YHPDHVKGL ILLGDIYIN NIKDLDAAE 
      NCYRRILEL DPENIQGLH NLCVVHVER GKLLEAQAC 
      LEKAHQLAP GEDYVLKHL QIVKNRIAK MKLNPDEVE 
      TEKSRSQVV SNKSIDESD RKTRPVVTG DSEEQRYSS 
      RVVNTEPMF VKNVDNIDY VDEDLGEVE FEEGMEQNV 
      SNKWRHGKV GTDNDDPSS GMS
    CDS sequence
    • >TC007431 RA
      atggggcac aaggcgatg ggggccatc gtggccctc 
      acctgcgtc ctctgctac tacaacagc tgctactgc 
      gggttcgtc ttcgacgac atcagtgcc atcaaagac 
      aacagagat ctgaggccg cattcgccg ctgcttaac 
      atttttttc aacgacttc tggggcacc cccatgcac 
      aaggagcag agccataag tcgtacagg cccttgtgt 
      gttttgact ttccgatgg aactatttg ctttggcag 
      ctggagccc atggggtat catctcgtg aatatgctc 
      ctccattcc gtcgtttgc ctcatgtac ttcaggatg 
      tgctccatg tttctaccg gagctgtca agtttcgtc 
      gcggccatg ttgttcgcc gttcatccc atccacacg 
      gaggcggtg acgggtgtc gtcggaagg gccgagacg 
      ctctcgtcg gtcttcttt ctggccgct tttatgctc 
      tatgccaaa gcgtccaaa agcaaaaaa tccacaggc 
      tggaaatac ctatcattt tccatgctg tcgatcgca 
      acggcgatg ctgtgcaaa gagcaggga atcacagtc 
      gctggcgta tgtgccgct tatgaaata ttcgtgacc 
      cagaaggtg cgtcttcca gagataaaa cacgtcgta 
      aaggcggcc atcacggcc aagtcctcc taccacctg 
      ccttggtcg agcgaggcc accaagagg ctcctggtg 
      ctttcggcg accactatg tgtcttttg ctggccagg 
      ctccagatc atgggctcc cagctgccg gtcttcacc 
      aggtttgat aacccggcc tcggtggca ccaacgccg 
      gccagacaa ctcacctac cactacctg ataagtgtc 
      aatctttgg ctgcttctg ttcccctgc aatctgtgc 
      tgcgactgg accatgggc accatcccc ttggtcgaa 
      accttcgca gatggacgg aatctggcc actattgcc 
      gcctacacc ttcttcacg gcgctgctg gtgaccgcc 
      tacacgacg gaaaaccgc caacagcgc accgtcata 
      cttatgagc ttggcttta atggccctc cccttcatg 
      cctgcatcc aacctcttt ttccccgtc ggctttgta 
      atcgccgaa cgagtgtta tacatgcct tcgatgggg 
      ttctgcatg ctggtggcc tacggatgg accgtcctc 
      gccgagaaa aagggcaaa aagctcgcc ttttttctc 
      ctaagcatc ctggttgtg acccacggt acaaaaacg 
      tacctccgg aactgggac tgggaggac gagtactcc 
      attttcatg tcggggttg cgagtcaat cagaaaaac 
      gccaagctt tttaataac gtggggcac gccctcgag 
      agccaaggc aagtacgag gaggcgtta aagttcttc 
      aaaacggcg gtgagtgtc caggaggac gacgtcgga 
      gcgcatatt aacgtcggg aggacttac aatcatctc 
      aagatgttc aaagaggct gaagaggcc tacctgcgg 
      gccaagtcg ctcttgccc aaagccaag cctggagag 
      agttaccag gcgaggatt gcgcctaat catctcaac 
      gttttcctc aacctggcg aatttaatt tcgaagaat 
      tcgacgaga ctggaagag gccgatatg ctctaccgg 
      caggccatc agtatgagg gctgattac actcaagct 
      tatattaat cgaggcgat attcttatc aagttgaac 
      aggacgaag gaggcgcaa gaggtctac gagagggcc 
      ctgctctac gacagcaac aaccctgat atttactac 
      aacctcggc gtggtgttc ctggagcag ggcaaggcg 
      tcccaagcc ctcgcttac ctggacaaa gctttagaa 
      ttcgatccg gagcacgag caggctctt ttgaattca 
      gcgatcctc cttcaagaa ttcaaccgg cccgaattg 
      cgtaaaatc gcccgcgaa cgtctattg aaacttcta 
      gacaaagac agcaacaac gagcgggtc catttcaat 
      ctcggaatg ctcgcaatg gacgagaaa aatatcgag 
      gaagccgaa cattggttc cggcgggct gtccattta 
      aaagccgat ttcaggagt tcgctcttc aatttagct 
      ttgttactg gccgacgat cagcggccc ctggaagcc 
      gccccgttc ctcaaccag ctcgtcaag tatcatccc 
      gatcacgtg aaaggactc atcctcctg ggggacatt 
      tatatcaat aacatcaag gacttggac gcggccgaa 
      aattgctat cgcaggatt ctcgagctc gacccggag 
      aacatccag ggtttgcac aacttgtgc gtggttcac 
      gtggagcga gggaagctt ctagaggcg caagcgtgt 
      ctggagaag gcgcaccaa ctggctcct ggggaggat 
      tacgtcttg aaacatctc caaatcgtc aagaatcgc 
      atcgcgaaa atgaaactt aatccagac gaagtcgag 
      acggagaaa agccggagt caggttgtt tcaaataaa 
      agtatagac gagagtgat cggaagacg cggcccgtg 
      gtcacggga gactccgag gagcagagg tatagcagt 
      agagtggta aacactgaa cccatgttt gtgaaaaat 
      gttgacaat attgactat gttgatgaa gatttggga 
      gaggtggag ttcgaggaa gggatggag caaaatgtt 
      agtaataag tggaggcat ggtaaggtc ggtactgac 
      aatgatgac ccgtcttca ggcatgtct tag
    mRNA sequence
    • >TC007431 RA
      gactccgga ggaatccgg tgcgcatat tccagaggg 
      tcacgtgag gcttcgacg cagtatggc agccatcac 
      accagttgg gttcttgcg acttagtgc tttcgaact 
      gttggctgg gtgtggacg tcccctcgt gcgtctgca 
      atgctcaca caactgtta cgtgcaagt ggtgcgagg 
      tgcagtgac ccggagtga tgctccact agcccccag 
      agtgcgaaa gacatgggg cacaaggcg atgggggcc 
      atcgtggcc ctcacctgc gtcctctgc tactacaac 
      agctgctac tgcgggttc gtcttcgac gacatcagt 
      gccatcaaa gacaacaga gatctgagg ccgcattcg 
      ccgctgctt aacattttt ttcaacgac ttctggggc 
      acccccatg cacaaggag cagagccat aagtcgtac 
      aggcccttg tgtgttttg actttccga tggaactat 
      ttgctttgg cagctggag cccatgggg tatcatctc 
      gtgaatatg ctcctccat tccgtcgtt tgcctcatg 
      tacttcagg atgtgctcc atgtttcta ccggagctg 
      tcaagtttc gtcgcggcc atgttgttc gccgttcat 
      cccatccac acggaggcg gtgacgggt gtcgtcgga 
      agggccgag acgctctcg tcggtcttc tttctggcc 
      gcttttatg ctctatgcc aaagcgtcc aaaagcaaa 
      aaatccaca ggctggaaa tacctatca ttttccatg 
      ctgtcgatc gcaacggcg atgctgtgc aaagagcag 
      ggaatcaca gtcgctggc gtatgtgcc gcttatgaa 
      atattcgtg acccagaag gtgcgtctt ccagagata 
      aaacacgtc gtaaaggcg gccatcacg gccaagtcc 
      tcctaccac ctgccttgg tcgagcgag gccaccaag 
      aggctcctg gtgctttcg gcgaccact atgtgtctt 
      ttgctggcc aggctccag atcatgggc tcccagctg 
      ccggtcttc accaggttt gataacccg gcctcggtg 
      gcaccaacg ccggccaga caactcacc taccactac 
      ctgataagt gtcaatctt tggctgctt ctgttcccc 
      tgcaatctg tgctgcgac tggaccatg ggcaccatc 
      cccttggtc gaaaccttc gcagatgga cggaatctg 
      gccactatt gccgcctac accttcttc acggcgctg 
      ctggtgacc gcctacacg acggaaaac cgccaacag 
      cgcaccgtc atacttatg agcttggct ttaatggcc 
      ctccccttc atgcctgca tccaacctc tttttcccc 
      gtcggcttt gtaatcgcc gaacgagtg ttatacatg 
      ccttcgatg gggttctgc atgctggtg gcctacgga 
      tggaccgtc ctcgccgag aaaaagggc aaaaagctc 
      gcctttttt ctcctaagc atcctggtt gtgacccac 
      ggtacaaaa acgtacctc cggaactgg gactgggag 
      gacgagtac tccattttc atgtcgggg ttgcgagtc 
      aatcagaaa aacgccaag ctttttaat aacgtgggg 
      cacgccctc gagagccaa ggcaagtac gaggaggcg 
      ttaaagttc ttcaaaacg gcggtgagt gtccaggag 
      gacgacgtc ggagcgcat attaacgtc gggaggact 
      tacaatcat ctcaagatg ttcaaagag gctgaagag 
      gcctacctg cgggccaag tcgctcttg cccaaagcc 
      aagcctgga gagagttac caggcgagg attgcgcct 
      aatcatctc aacgttttc ctcaacctg gcgaattta 
      atttcgaag aattcgacg agactggaa gaggccgat 
      atgctctac cggcaggcc atcagtatg agggctgat 
      tacactcaa gcttatatt aatcgaggc gatattctt 
      atcaagttg aacaggacg aaggaggcg caagaggtc 
      tacgagagg gccctgctc tacgacagc aacaaccct 
      gatatttac tacaacctc ggcgtggtg ttcctggag 
      cagggcaag gcgtcccaa gccctcgct tacctggac 
      aaagcttta gaattcgat ccggagcac gagcaggct 
      cttttgaat tcagcgatc ctccttcaa gaattcaac 
      cggcccgaa ttgcgtaaa atcgcccgc gaacgtcta 
      ttgaaactt ctagacaaa gacagcaac aacgagcgg 
      gtccatttc aatctcgga atgctcgca atggacgag 
      aaaaatatc gaggaagcc gaacattgg ttccggcgg 
      gctgtccat ttaaaagcc gatttcagg agttcgctc 
      ttcaattta gctttgtta ctggccgac gatcagcgg 
      cccctggaa gccgccccg ttcctcaac cagctcgtc 
      aagtatcat cccgatcac gtgaaagga ctcatcctc 
      ctgggggac atttatatc aataacatc aaggacttg 
      gacgcggcc gaaaattgc tatcgcagg attctcgag 
      ctcgacccg gagaacatc cagggtttg cacaacttg 
      tgcgtggtt cacgtggag cgagggaag cttctagag 
      gcgcaagcg tgtctggag aaggcgcac caactggct 
      cctggggag gattacgtc ttgaaacat ctccaaatc 
      gtcaagaat cgcatcgcg aaaatgaaa cttaatcca 
      gacgaagtc gagacggag aaaagccgg agtcaggtt 
      gtttcaaat aaaagtata gacgagagt gatcggaag 
      acgcggccc gtggtcacg ggagactcc gaggagcag 
      aggtatagc agtagagtg gtaaacact gaacccatg 
      tttgtgaaa aatgttgac aatattgac tatgttgat 
      gaagatttg ggagaggtg gagttcgag gaagggatg 
      gagcaaaat gttagtaat aagtggagg catggtaag 
      gtcggtact gacaatgat gacccgtct tcaggcatg 
      tcttagcat taaaagagt attttcgaa tcaaattaa 
      tgtaatttt aatgagccc ccgtaaggg gttttactc 
      tatctaagg actttttta tacgagcca ttagatgta 
      gtgtccatt ctgtgatac tcagaataa tcgtttttg 
      aatctattt ttgatctgg ttttgttcg atctaagac 
      ctatgcaga tgtgactta atgtataca agattttcg 
      cacttgttt tttgtgatt tttcattgt gattcattt 
      tgtgattag ctttatgta tcgaggtgt tttgtcgct 
      tgttttgct taaatcaaa tgttactta atgtgatta 
      tttgattcc tgtcgattg tttgtcggt aagtggcat 
      atctgtaat atgttaaat tagtaaata ttgctacta 
      atataatca ataaaaata aatgtattg atgtaaggc 
      ttgttagcc gctagtttt agacgacat ccacgtggc 
      caaatattc agtagaaat ccttagcac ttctcaatc 
      atttgtcta ttttttcgg gtgaaaaaa ttgttattt 
      ttgctatcg tggatctta atttatttc gtattttgt 
      aaaggtgcc ttggaatcg ttaattaag gcaaatgat 
      tttgtaata tagtataat aaatatttt tctatgttg 
      tcaaactgt tcatagatt ataaacata agtctatat 
      tgtgtaaaa ctaatgtga gaggtatat ttatcctat 
      gactgataa gttttcgtg tattttctt tcgaaattt 
      tctaaaagt tgttaccct aatatacac ttttttaat 
      aaaaccagt tgtggtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY