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Gene information
General information
TC007423 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 4744843..4745820
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007423
    iBeetle screen iB_01185
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen dorsoventral-axis shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 5


    elytra slightly blistered - Number of animals showing the phenotype: 5


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007423 RA
      MSLTIQQII TDAKRLAGR LKDRDTIAD 
      TLLNETNAI TKKIDAMKQ FQEEVDVLN 
      EVANQKPHS QLIANIQKE NRHLREIQQ 
      ENRELRAAL EDYQTTLEH IMTKYREHT 
      TEEIYKTRI DFKALQDKQ YDEIIKHQA 
      EKIHEMAAV MEQAALIDE DRMVCNEEI 
      IARLKLENQ GLREMLGIA FKHGNLINK 
      MEDKTIQTE TST
    CDS sequence
    • >TC007423 RA
      atgtccctc acaattcag caaataatc actgacgcg 
      aaacgccta gcaggtcgt ttgaaagac cgcgataca 
      attgctgat actttgttg aatgagacg aacgcaata 
      acgaaaaaa attgatgca atgaaacag tttcaagaa 
      gaagtcgat gtgttgaac gaggtggcg aaccaaaag 
      ccgcattcc caattaatt gcgaatata caaaaggag 
      aatagacac ttgagggag attcagcag gagaatagg 
      gagctgagg gcagcgtta gaagattat caaaccaca 
      cttgaacat attatgacc aagtatcgg gagcacacc 
      acggaggaa atctacaag actaggata gacttcaag 
      gcgttgcaa gacaagcaa tacgacgag ataatcaag 
      caccaggcc gagaaaata cacgaaatg gccgcagtt 
      atggagcag gctgccctg attgacgag gacaggatg 
      gtgtgcaac gaggaaata atcgcaagg ctcaaattg 
      gaaaaccag ggcctaagg gagatgtta ggtattgca 
      tttaaacac ggcaattta attaataaa atggaagac 
      aagaccatc caaactgag acctcaact tga
    mRNA sequence
    • >TC007423 RA
      ggccttgtg tgtttcctt caacctaaa ttatttatt 
      tgtccgtct gtcaattac aattgtcca tattttaaa 
      caaatgctg agaattcct caatgaaat aagcgaaaa 
      caaaatgcc tacagtgta gttatgtgt ttttaaagt 
      gattttttt accccggtg gccactaag ttacctcat 
      aaaaaatgt ccctcacaa ttcagcaaa taatcactg 
      acgcgaaac gcctagcag gtcgtttga aagaccgcg 
      atacaattg ctgatactt tgttgaatg agacgaacg 
      caataacga aaaaaattg atgcaatga aacagtttc 
      aagaagaag tcgatgtgt tgaacgagg tggcgaacc 
      aaaagccgc attcccaat taattgcga atatacaaa 
      aggagaata gacacttga gggagattc agcaggaga 
      atagggagc tgagggcag cgttagaag attatcaaa 
      ccacacttg aacatatta tgaccaagt atcgggagc 
      acaccacgg aggaaatct acaagacta ggatagact 
      tcaaggcgt tgcaagaca agcaatacg acgagataa 
      tcaagcacc aggccgaga aaatacacg aaatggccg 
      cagttatgg agcaggctg ccctgattg acgaggaca 
      ggatggtgt gcaacgagg aaataatcg caaggctca 
      aattggaaa accagggcc taagggaga tgttaggta 
      ttgcattta aacacggca atttaatta ataaaatgg 
      aagacaaga ccatccaaa ctgagacct caacttgaa 
      tttcccctc acgatttta tccatgttt tgttctgtt 
      ttacttatg tttatatac ttttgcact gttaataaa 
      att