Status
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Gene information
General information
TC007422 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4746538..4750990
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007422
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01184

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007422 RA
      MAQPNQQNG MPPQMKPPI YNGVHSSHS SRDSSPHGK 
      NIPLSQLPP RSAIPPTQL PPSRSPNIT QQLSQLNIN 
      KFNTSTPNQ SPNPSGLVS SAFSPIGST QAPPRTTQA 
      PPTGYNFTQ KPPSTQGQM PVITNQQPQ QKPPTIQNQ 
      VQVPPPNQG QAPPSTQNQ MPPPVKNQS FVPPPHNFN 
      SSPINQGQA PPPAQSQMP PPIKNPNFV PPPHNFNSI 
      ANQGVAPPT TQGHTQIPP PIKSQSFVL PPSSVINQG 
      VTPPPTQGQ GQVPPPIKN QSFVPPPHN FNSSSINQG 
      MAPPPTQGQ SQMPPSIKS QAFVPPPHN FNSTPLTQG 
      QSQVSSSQN FSSMQPPPI QGQIPPPNS QIHNNFNSS 
      PSNQGYRPP PNQVQMPPP LSQGYQPPP VETNLPSNQ 
      FNNSSPRLP PPGAPKANL HSNRYPQGP PPQQSYNPQ 
      APPSFNPQT QNYPVSAQT QQQFYQQNS VTQSGFNKL 
      WGHDHFDLL QNPNVLPPE KVAPPKVYL GNELLDSAN 
      CSPEVFRCT MTKIPESNS LLQKSRLPL GILIHPFKD 
      LTHLPVIQC NVIVRCRAC RTYINPFVY FIDTKRWKC 
      NLCYRVNEL PEEFQFDPV SKTYGDPTR RPEIKSSTI 
      EYIAPAEYM LRPPQPAVY LFLFDISRL AIESGYLQS 
      ACAVLLEEL KSLPGDART QVGILAYDS ALHFFGLGE 
      GLSQPHEMT VLEIDDIFL PCPDNLLVN LKDRIDLLT 
      DLLTQLPTR YNNSYDNNS ALGAALQAA HKMMGATGG 
      RVSVFQASL PNVGPGALV PREDPSQRA STEVQHLNP 
      ANDFYKRLA LECSGQQIA VDLFVLNSQ YVDMATISG 
      ISRFSGGCI HHFPLFKAS RVLQNERFE RCFRRYLVR 
      KIGFEAVMR IRCTRGLSI HTFHGNFFV RSTDLLSLP 
      NVSPDAAFG MQVSIDETL SDVQSVCFQ AALLYTSSK 
      GERRIRVHT LCLPVCATL SDVINAADQ QCIVGLLAK 
      MAVDRSMQS SLSDAREAF INVAIDILS SYKMSLNMA 
      EGPTSLLAP PCLRLLPLY ISALLKSTA FRTGTCTRL 
      DDRVKAMID MKTKPLGHL IQMIYPDLY PVHNLEEQP 
      IVLNSEEEP IPQPPHLQL TARCLDSKG AFLMDTGEH 
      MILLVAPGV SPMFLSEAL GVRDYMSIT DEMYELPVL 
      DNLHNQRLH QFINFLNDE KPAAATLHV IRDNSPNRI 
      EFYERLIED RVENALSYH EFMQHLKTQ VK
    CDS sequence
    • >TC007422 RA
      atggctcaa ccgaatcag caaaatggt atgcccccc 
      caaatgaag cccccaatc tacaatgga gttcattcg 
      tcgcattcg tcacgcgac tcgtccccc catggcaaa 
      aatatcccc ctttcacag ctgcctcca cgctccgcc 
      atcccccca acacaactc ccgccatct agatcccct 
      aatataact caacagttg agccaactc aacatcaac 
      aaatttaac acttcaaca cctaatcaa tcaccgaat 
      ccaagtgga ctagtcagt agcgcattt tcgccaatt 
      ggaagcacc caagcgccg cccaggacc acgcaagcc 
      ccacccacc ggctacaac ttcacccaa aagccgccc 
      tcaacacaa ggacaaatg ccagtaatt accaatcaa 
      cagcctcaa caaaaacca ccaacaata caaaatcaa 
      gtccaagtc ccgccccca aatcaaggc caggccccg 
      ccctcaacg cagaaccaa atgccgcct ccagtaaaa 
      aatcagagc tttgttccg ccgccacac aactttaat 
      tcaagtcct attaatcaa ggtcaggcc ccgcctccg 
      gcgcagagt cagatgccg ccccctatc aaaaatccg 
      aattttgtt ccgcctcct cacaatttt aactctatt 
      gcaaatcaa ggtgtggcg ccgcctaca acgcaaggt 
      cacactcaa ataccgccc ccaataaaa agtcagagc 
      tttgttctg cctccaagt tctgttata aatcaaggt 
      gtgacaccg cccccaacg caaggtcag ggccaagta 
      ccgcctcca ataaaaaat cagagtttt gttccgccg 
      ccacacaac tttaattca agttctatt aatcaaggt 
      atggcaccg cccccaacg caaggtcag agccaaatg 
      ccaccttca ataaaaagt caggccttt gttccgccg 
      ccacacaat tttaattca actccttta acgcaaggc 
      caaagtcaa gtctcgtct tcacagaat tttagttca 
      atgcagccg cccccaatc caaggccaa ataccacct 
      ccgaatagc caaattcat aataatttt aattcgagt 
      cctagtaat caaggatat cggccgcct ccaaatcaa 
      gtccaaatg ccaccgcct ttaagtcaa ggctatcaa 
      ccgccacca gttgaaact aatttgcct tcaaaccaa 
      tttaacaat tcgagtcca cggctgccg cctccgggg 
      gcacctaag gccaatctg cactcaaac agataccca 
      caagggcca ccccctcag cagagttat aaccctcaa 
      gccccgcct agtttcaac ccccagaca caaaattac 
      cccgtctcg gcacagacg cagcaacag ttctatcaa 
      cagaacagc gtgactcaa tctggtttt aacaaatta 
      tggggccac gaccacttt gatctcctt caaaatccg 
      aacgttcta cctccggag aaagtggcg ccacctaaa 
      gtctactta gggaatgag cttctcgat tcggcgaat 
      tgcagtcca gaagttttc cgatgtacg atgacgaaa 
      atccccgaa agcaactct ctgttgcag aagtcgcgt 
      ctaccctta ggaattttg attcatccc tttaaagac 
      ttgactcat ttgccggta attcaatgt aacgttatc 
      gtgagatgt cgcgcctgt cgcacctac atcaacccc 
      tttgtttat ttcatcgac acgaagagg tggaaatgt 
      aatttgtgc tatcgagtg aacgaattg cccgaagaa 
      tttcagttc gaccctgtt agtaaaaca tacggggac 
      ccgacaaga agaccggag atcaagtct agtactatc 
      gagtacata gcaccagcg gaatacatg ttaaggcct 
      ccacaaccg gctgtctat ttgtttttg ttcgacatt 
      tcccggctt gccatcgag agtggctac ttgcaaagc 
      gcatgtgct gtgcttttg gaagaactg aaaagtttg 
      ccaggcgat gcccgcacg caagtcggc atattagcc 
      tacgactcg gctttacat ttctttgga ctcggcgaa 
      gggttgagc caaccccac gaaatgacg gtgcttgaa 
      atcgacgat atatttctg ccttgtccc gacaacctc 
      ctagtgaac ttgaaggac cgaatcgac ttacttacc 
      gatctgttg acccaacta ccaacgcgt tacaacaac 
      agttacgac aacaattcg gctctgggg gctgcctta 
      caagcggcg cataaaatg atgggggct accggaggc 
      cgcgttagc gttttccaa gcgtcgttg cccaacgtc 
      gggcccggg gctttggtc ccgagggag gatccctcc 
      cagagggct tcgaccgaa gtccagcac ttgaaccca 
      gccaatgac ttctacaaa cgcttggct ttggagtgc 
      agtgggcag cagatcgcc gttgatttg ttcgttctg 
      aactcgcag tatgtggac atggcgact atatcgggg 
      attagccgg tttagcggc ggttgcatc caccacttc 
      cccctgttc aaggcatca agagtgttg cagaacgag 
      cggttcgaa cggtgcttc cgccggtat ttagtgcgg 
      aagatcggg tttgaggct gtgatgcgt attcggtgc 
      actcgcggg ttatcaatt cacacgttc catggcaac 
      ttcttcgtg cgttcgacc gatttgcta tcgctgcca 
      aatgtgagt cctgatgct gcgttcggt atgcaagtc 
      tcgattgac gagaccctg tcggatgtg cagtcagtc 
      tgcttccaa gcggctctt ctgtacact tcgagcaag 
      ggagaacgg aggataagg gtgcacact ttgtgcctt 
      ccggtgtgc gccacccta tccgacgtg atcaatgcg 
      gccgatcag cagtgtatt gtgggcttg ctggccaag 
      atggcagtt gataggtcc atgcagtct agtctttcg 
      gacgcacgg gaagcgttc ataaacgtg gccatagat 
      atactttcg agctacaag atgtcgctg aatatggcc 
      gaaggcccc acctcgtta ctcgcgccc ccttgtttg 
      cggttattg cctttgtac atatcggcc ttactgaaa 
      tcgacggct ttccggacc gggacttgc acccgactg 
      gacgatcgc gtcaaagca atgatcgat atgaagacc 
      aaaccgtta ggacatttg atacaaatg atatatccc 
      gacttgtat cccgtacat aacttggag gaacagccc 
      atagtcctg aacagtgaa gaggaacca atcccgcaa 
      ccaccccac ttgcaatta accgcgagg tgtctggac 
      tccaaaggc gcgttcctt atggatacc ggggagcat 
      atgatcctc ttagtcgca ccgggagtg tccccgatg 
      ttcctgagt gaggcgcta ggtgtccgg gattacatg 
      tctatcact gacgagatg tacgaattg cccgttctg 
      gataatctt cacaaccaa agactgcat caatttata 
      aattttttg aacgacgag aaacccgct gcagctacg 
      ttgcatgtc attagagat aatagtccg aatagaata 
      gaattctac gaaaggtta atcgaagat agggtagag 
      aatgcactc agttatcac gagtttatg caacatctc 
      aaaactcaa gttaaatga
    mRNA sequence
    • >TC007422 RA
      gcggccacg taagattga caataatga caaccagaa 
      attgtctcg taaagaaat tattgttga attttgata 
      caaacgcca cgaattgaa taggaaata gccaatgaa 
      tctattaaa ttaaacaaa atggctcaa ccgaatcag 
      caaaatggt atgcccccc caaatgaag cccccaatc 
      tacaatgga gttcattcg tcgcattcg tcacgcgac 
      tcgtccccc catggcaaa aatatcccc ctttcacag 
      ctgcctcca cgctccgcc atcccccca acacaactc 
      ccgccatct agatcccct aatataact caacagttg 
      agccaactc aacatcaac aaatttaac acttcaaca 
      cctaatcaa tcaccgaat ccaagtgga ctagtcagt 
      agcgcattt tcgccaatt ggaagcacc caagcgccg 
      cccaggacc acgcaagcc ccacccacc ggctacaac 
      ttcacccaa aagccgccc tcaacacaa ggacaaatg 
      ccagtaatt accaatcaa cagcctcaa caaaaacca 
      ccaacaata caaaatcaa gtccaagtc ccgccccca 
      aatcaaggc caggccccg ccctcaacg cagaaccaa 
      atgccgcct ccagtaaaa aatcagagc tttgttccg 
      ccgccacac aactttaat tcaagtcct attaatcaa 
      ggtcaggcc ccgcctccg gcgcagagt cagatgccg 
      ccccctatc aaaaatccg aattttgtt ccgcctcct 
      cacaatttt aactctatt gcaaatcaa ggtgtggcg 
      ccgcctaca acgcaaggt cacactcaa ataccgccc 
      ccaataaaa agtcagagc tttgttctg cctccaagt 
      tctgttata aatcaaggt gtgacaccg cccccaacg 
      caaggtcag ggccaagta ccgcctcca ataaaaaat 
      cagagtttt gttccgccg ccacacaac tttaattca 
      agttctatt aatcaaggt atggcaccg cccccaacg 
      caaggtcag agccaaatg ccaccttca ataaaaagt 
      caggccttt gttccgccg ccacacaat tttaattca 
      actccttta acgcaaggc caaagtcaa gtctcgtct 
      tcacagaat tttagttca atgcagccg cccccaatc 
      caaggccaa ataccacct ccgaatagc caaattcat 
      aataatttt aattcgagt cctagtaat caaggatat 
      cggccgcct ccaaatcaa gtccaaatg ccaccgcct 
      ttaagtcaa ggctatcaa ccgccacca gttgaaact 
      aatttgcct tcaaaccaa tttaacaat tcgagtcca 
      cggctgccg cctccgggg gcacctaag gccaatctg 
      cactcaaac agataccca caagggcca ccccctcag 
      cagagttat aaccctcaa gccccgcct agtttcaac 
      ccccagaca caaaattac cccgtctcg gcacagacg 
      cagcaacag ttctatcaa cagaacagc gtgactcaa 
      tctggtttt aacaaatta tggggccac gaccacttt 
      gatctcctt caaaatccg aacgttcta cctccggag 
      aaagtggcg ccacctaaa gtctactta gggaatgag 
      cttctcgat tcggcgaat tgcagtcca gaagttttc 
      cgatgtacg atgacgaaa atccccgaa agcaactct 
      ctgttgcag aagtcgcgt ctaccctta ggaattttg 
      attcatccc tttaaagac ttgactcat ttgccggta 
      attcaatgt aacgttatc gtgagatgt cgcgcctgt 
      cgcacctac atcaacccc tttgtttat ttcatcgac 
      acgaagagg tggaaatgt aatttgtgc tatcgagtg 
      aacgaattg cccgaagaa tttcagttc gaccctgtt 
      agtaaaaca tacggggac ccgacaaga agaccggag 
      atcaagtct agtactatc gagtacata gcaccagcg 
      gaatacatg ttaaggcct ccacaaccg gctgtctat 
      ttgtttttg ttcgacatt tcccggctt gccatcgag 
      agtggctac ttgcaaagc gcatgtgct gtgcttttg 
      gaagaactg aaaagtttg ccaggcgat gcccgcacg 
      caagtcggc atattagcc tacgactcg gctttacat 
      ttctttgga ctcggcgaa gggttgagc caaccccac 
      gaaatgacg gtgcttgaa atcgacgat atatttctg 
      ccttgtccc gacaacctc ctagtgaac ttgaaggac 
      cgaatcgac ttacttacc gatctgttg acccaacta 
      ccaacgcgt tacaacaac agttacgac aacaattcg 
      gctctgggg gctgcctta caagcggcg cataaaatg 
      atgggggct accggaggc cgcgttagc gttttccaa 
      gcgtcgttg cccaacgtc gggcccggg gctttggtc 
      ccgagggag gatccctcc cagagggct tcgaccgaa 
      gtccagcac ttgaaccca gccaatgac ttctacaaa 
      cgcttggct ttggagtgc agtgggcag cagatcgcc 
      gttgatttg ttcgttctg aactcgcag tatgtggac 
      atggcgact atatcgggg attagccgg tttagcggc 
      ggttgcatc caccacttc cccctgttc aaggcatca 
      agagtgttg cagaacgag cggttcgaa cggtgcttc 
      cgccggtat ttagtgcgg aagatcggg tttgaggct 
      gtgatgcgt attcggtgc actcgcggg ttatcaatt 
      cacacgttc catggcaac ttcttcgtg cgttcgacc 
      gatttgcta tcgctgcca aatgtgagt cctgatgct 
      gcgttcggt atgcaagtc tcgattgac gagaccctg 
      tcggatgtg cagtcagtc tgcttccaa gcggctctt 
      ctgtacact tcgagcaag ggagaacgg aggataagg 
      gtgcacact ttgtgcctt ccggtgtgc gccacccta 
      tccgacgtg atcaatgcg gccgatcag cagtgtatt 
      gtgggcttg ctggccaag atggcagtt gataggtcc 
      atgcagtct agtctttcg gacgcacgg gaagcgttc 
      ataaacgtg gccatagat atactttcg agctacaag 
      atgtcgctg aatatggcc gaaggcccc acctcgtta 
      ctcgcgccc ccttgtttg cggttattg cctttgtac 
      atatcggcc ttactgaaa tcgacggct ttccggacc 
      gggacttgc acccgactg gacgatcgc gtcaaagca 
      atgatcgat atgaagacc aaaccgtta ggacatttg 
      atacaaatg atatatccc gacttgtat cccgtacat 
      aacttggag gaacagccc atagtcctg aacagtgaa 
      gaggaacca atcccgcaa ccaccccac ttgcaatta 
      accgcgagg tgtctggac tccaaaggc gcgttcctt 
      atggatacc ggggagcat atgatcctc ttagtcgca 
      ccgggagtg tccccgatg ttcctgagt gaggcgcta 
      ggtgtccgg gattacatg tctatcact gacgagatg 
      tacgaattg cccgttctg gataatctt cacaaccaa 
      agactgcat caatttata aattttttg aacgacgag 
      aaacccgct gcagctacg ttgcatgtc attagagat 
      aatagtccg aatagaata gaattctac gaaaggtta 
      atcgaagat agggtagag aatgcactc agttatcac 
      gagtttatg caacatctc aaaactcaa gttaaatga 
      aaagagggg acgggtcat gttgatgca aaattattg 
      ctcaaaaac ggggggagg agtttaacg cgtctaagg 
      ttttacttt taaggttga ctttatttt gatatttat 
      atattgtga tatgtatct ttatattca cataaaatg 
      tatagcaat atttttgca ttaatctca tgctttgtt 
      tattgtacg ccgtcagaa ctcatcgtt cattatgtt 
      ttgatgtct atcgatcat tctctgtta taggttcag 
      ccgcgttat tttgtccta caggttatg aaattgtct 
      gttggtacc aatataagt tgtaaaaat aaagaaatg 
      tatataat
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