Status
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Gene information
General information
TC007403 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5018342..5021011
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007403
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01180

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007403 RA
      MGRKVTVAS STLNQWALD FDGNRERIL ESILEAKDL 
      GATFRTGPE LEICGYSCE DHFYESDTF LHCWEVLLE 
      LLTAPLCKD MIIDVGMPV MHKNVAYNC RVVFLNQKI 
      LLIRPKLKM CDDGNYRES RWFAPWKKI RQTEDYFLP 
      RMISKFTGQ STVPIGDAV IATRDTCLG FEICEELWN 
      PASSHINMA LDGVEIISN SSGSYTELR KAFVSVDLV 
      KSATFKAGG CYIFSNLRG CDGQRVYFG GCSCVALNG 
      NIISRAKQF ALQDVEVTV ATLDLEDIR SYRNQIRSL 
      AHLAAESPS YPRVVVDFS LSQEQDATL PIASPIEWV 
      YLRPEEEIA QGPACWLWD YLRRSGQGG FFLPLSGGV 
      DSSSVALIV FSMCKMLVE AVQRGDNRV LSDLRRVLG 
      DPEYTPRTP SELCNRILV TCYMGTENS SKETKQRAA 
      TLAASIGSY HMHIMIDKA ITAIIEIFS GVTGLFPKF 
      ASRGGCPRQ NLALQNIQA RLRMVLSYL FAQLMLWAR 
      NRPGGLLVL GSANVDEAL RGYMTKYDC SSADINPIG 
      GISKTDLRR FLNYAKSKF EIPVIGEIV DAPPTAELE 
      PLQDGKLAQ TDEEDMGMT YAELSQFGR LRKIEKCGP 
      FSMYCKLVN TWGGNCTPR EVAEKVKHF FRCYAINRH 
      KMTVLTPAY HAEQYSPDD NRFDHRPFL YRANWSWQF 
      RAIDKQLEY QGTSKRSIT NVGQQANKK VDNTARIRT 
      GIAV
    CDS sequence
    • >TC007403 RA
      atgggccga aaagtgact gtggccagc tccaccctc 
      aaccaatgg gccttagat ttcgacgga aatcgcgaa 
      cgaatcctc gaatccatt ctcgaggcc aaagacctg 
      ggggccacc ttccgcaca ggccccgaa ttggaaatt 
      tgcggctac agctgcgag gatcatttc tacgaaagc 
      gatacgttt ttgcactgc tgggaagtc ctccttgaa 
      ctgctaaca gccccctta tgcaaagac atgataatc 
      gatgtcggt atgcccgtc atgcacaaa aacgtggct 
      tacaattgt cgcgttgtt tttctcaac caaaaaata 
      ctcttaatt cgtccgaaa ctaaaaatg tgtgatgat 
      gggaattac agggagtcg cggtggttc gccccgtgg 
      aaaaaaatc cgacaaacg gaggattat ttcctccct 
      aggatgatt tcaaaattt acagggcaa agcactgtg 
      ccgattggt gacgcggtg attgcgacg cgtgacact 
      tgtctcggg tttgaaatt tgcgaggag ttgtggaac 
      ccggccagt agtcacatt aatatggct ctggacggg 
      gttgaaata atttcaaat agttcaggg agttacact 
      gagcttcgc aaagctttt gtgtcggtt gatttggtc 
      aaaagcgca acttttaaa gccggcggt tgttacatt 
      tttagtaat ttgcgcggt tgcgatggg caaagagtt 
      tatttcggg ggctgttcg tgcgtagcc ctcaacgga 
      aatatcata agtcgggcc aaacaattc gcactgcaa 
      gatgttgaa gtcactgtt gcgactctt gatttggaa 
      gatataaga tcgtaccgg aaccaaatc cggtctttg 
      gctcacttg gccgctgag agtccgtcg taccctcga 
      gtggtagtt gatttctcc ttgtcgcaa gaacaggac 
      gcaactctt ccgattgct agccccatt gaatgggtg 
      tacttgcga ccggaagag gagatagcg caaggacca 
      gcttgttgg ttgtgggat tatttgaga aggtcggga 
      caagggggc tttttcttg cctttgagt ggcggcgtg 
      gactcgtcg agtgtcgcc ctcattgtt ttctcaatg 
      tgcaaaatg ctagtagag gcagtacag cgcggagac 
      aatcgtgtc ttatccgat ttgcgtcgc gtcctgggg 
      gaccctgaa tacacccct cgcacccct tcagagcta 
      tgcaatcgc atcctcgtc acttgttac atgggcact 
      gagaactcc tccaaagag acaaaacaa cgcgctgca 
      actttagcc gcctcaatc ggcagttac cacatgcac 
      ataatgata gataaggcc ataacggct attatcgag 
      attttcagt ggggttaca ggcctattc ccgaaattc 
      gcctctcgt gggggctgc cctcggcaa aacctcgcc 
      ctccaaaac atccaagcg cgcctccgc atggtcctg 
      tcttacctg tttgcgcaa ctgatgttg tgggcccgc 
      aatcggcct ggggggctc ctggtgcta ggctccgcg 
      aacgtggat gaggcgcta aggggctac atgacgaaa 
      tatgactgc tcgagcgct gatattaac ccgattggt 
      ggcatatct aagactgac ttaaggcgg tttttgaat 
      tatgccaaa tcaaaattt gagattcct gtcattggg 
      gagattgtg gatgcgccc ccgacggcc gaattggag 
      cccctgcag gacgggaag ttggcccaa accgacgag 
      gaggacatg gggatgacg tacgccgaa ctgagccag 
      ttcggccga ttgcgcaaa attgaaaag tgtggccct 
      ttttccatg tattgcaaa ttagtcaac acgtggggt 
      gggaattgt accccgcgt gaagtggcc gagaaagtc 
      aagcatttt ttcagatgc tatgcaatt aataggcac 
      aaaatgacg gtgttaaca ccggcttat cacgcggaa 
      cagtacagt cccgatgat aataggttt gatcatagg 
      ccgtttttg tatcgggcg aattggtcg tggcagttt 
      cgggctatt gacaaacaa ttggaatat caaggtacg 
      tccaaaagg tcgataacg aatgtggga caacaggcg 
      aacaagaag gtggacaat acggcaagg attagaacc 
      ggaattgcc gtttga
    mRNA sequence
    • >TC007403 RA
      tgtgggtaa accccccaa aaacttttt tttttgaaa 
      aatttgaaa aaacgtgcg ctctctggt ggccgacac 
      cctcatcat ttctaacct cagaatcct ctaaatcca 
      taaagactg aaaatgtct acataaaca gctatcgaa 
      caaacagtc ttatcgcga tatcgcccc ctactccat 
      tcatttcat tccaaatcc gattttagc tctttaaaa 
      aaatgggcc gaaaagtga ctgtggcca gctccaccc 
      tcaaccaat gggccttag atttcgacg gaaatcgcg 
      aacgaatcc tcgaatcca ttctcgagg ccaaagacc 
      tgggggcca ccttccgca caggccccg aattggaaa 
      tttgcggct acagctgcg aggatcatt tctacgaaa 
      gcgatacgt ttttgcact gctgggaag tcctccttg 
      aactgctaa cagccccct tatgcaaag acatgataa 
      tcgatgtcg gtatgcccg tcatgcaca aaaacgtgg 
      cttacaatt gtcgcgttg tttttctca accaaaaaa 
      tactcttaa ttcgtccga aactaaaaa tgtgtgatg 
      atgggaatt acagggagt cgcggtggt tcgccccgt 
      ggaaaaaaa tccgacaaa cggaggatt atttcctcc 
      ctaggatga tttcaaaat ttacagggc aaagcactg 
      tgccgattg gtgacgcgg tgattgcga cgcgtgaca 
      cttgtctcg ggtttgaaa tttgcgagg agttgtgga 
      acccggcca gtagtcaca ttaatatgg ctctggacg 
      gggttgaaa taatttcaa atagttcag ggagttaca 
      ctgagcttc gcaaagctt ttgtgtcgg ttgatttgg 
      tcaaaagcg caactttta aagccggcg gttgttaca 
      tttttagta atttgcgcg gttgcgatg ggcaaagag 
      tttatttcg ggggctgtt cgtgcgtag ccctcaacg 
      gaaatatca taagtcggg ccaaacaat tcgcactgc 
      aagatgttg aagtcactg ttgcgactc ttgatttgg 
      aagatataa gatcgtacc ggaaccaaa tccggtctt 
      tggctcact tggccgctg agagtccgt cgtaccctc 
      gagtggtag ttgatttct ccttgtcgc aagaacagg 
      acgcaactc ttccgattg ctagcccca ttgaatggg 
      tgtacttgc gaccggaag aggagatag cgcaaggac 
      cagcttgtt ggttgtggg attatttga gaaggtcgg 
      gacaagggg gctttttct tgcctttga gtggcggcg 
      tggactcgt cgagtgtcg ccctcattg ttttctcaa 
      tgtgcaaaa tgctagtag aggcagtac agcgcggag 
      acaatcgtg tcttatccg atttgcgtc gcgtcctgg 
      gggaccctg aatacaccc ctcgcaccc cttcagagc 
      tatgcaatc gcatcctcg tcacttgtt acatgggca 
      ctgagaact cctccaaag agacaaaac aacgcgctg 
      caactttag ccgcctcaa tcggcagtt accacatgc 
      acataatga tagataagg ccataacgg ctattatcg 
      agattttca gtggggtta caggcctat tcccgaaat 
      tcgcctctc gtgggggct gccctcggc aaaacctcg 
      ccctccaaa acatccaag cgcgcctcc gcatggtcc 
      tgtcttacc tgtttgcgc aactgatgt tgtgggccc 
      gcaatcggc ctggggggc tcctggtgc taggctccg 
      cgaacgtgg atgaggcgc taaggggct acatgacga 
      aatatgact gctcgagcg ctgatatta acccgattg 
      gtggcatat ctaagactg acttaaggc ggtttttga 
      attatgcca aatcaaaat ttgagattc ctgtcattg 
      gggagattg tggatgcgc ccccgacgg ccgaattgg 
      agcccctgc aggacggga agttggccc aaaccgacg 
      aggaggaca tggggatga cgtacgccg aactgagcc 
      agttcggcc gattgcgca aaattgaaa agtgtggcc 
      ctttttcca tgtattgca aattagtca acacgtggg 
      gtgggaatt gtaccccgc gtgaagtgg ccgagaaag 
      tcaagcatt ttttcagat gctatgcaa ttaataggc 
      acaaaatga cggtgttaa caccggctt atcacgcgg 
      aacagtaca gtcccgatg ataataggt ttgatcata 
      ggccgtttt tgtatcggg cgaattggt cgtggcagt 
      ttcgggcta ttgacaaac aattggaat atcaaggta 
      cgtccaaaa ggtcgataa cgaatgtgg gacaacagg 
      cgaacaaga aggtggaca atacggcaa ggattagaa 
      ccggaattg ccgtttgaa ataatgtta agaaaaaaa 
      attattatt ccgcttgtt ccttaattg tgatttttg 
      tcttatttt taataaaag caagttttt act
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY