Status
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Gene information
General information
TC007374 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5377435..5379834
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007374
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01178

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head capsule posterior potentiallyconstricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule posterior potentiallystructure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007374 RA
      MLKLSKLFN PISKHLRNL QPRLCFSSD SEVPVFDHF 
      YDCLVIGAG GAGLRAAYG LTAKGFNTA VVTKIFPTR 
      SHTVSAQGG INAALGNYE PDKWYWHFF DTVKGSDWL 
      GDQDAIHYM TKNAPSAIL ELENVGMPF SRFKDGRIY 
      QRAFGGQSL ECGKGGQAH RTCAAADST GQFMLHTLY 
      GQAVKYNCK FFVEYFALD LIMDDERCC GAITWDLSS 
      GTFHRFFAR NTVIATGGY ERAYFSCTT AHTSTGDGT 
      AMVSRAGLP LQDLEFVQF HPTGIYGVG VLVTEGARG 
      EGGYITNSK GERFMERYA PHAKDLASR DVVSRSCAM 
      EILEGRGVG PEKDHVHLE LMHLPHELI RKQLPGISN 
      LVAGFVGRD VTKEPIPIV PTVHYNMGG IPTNYKGQV 
      ITQSPEKCE QLVTGLYAC GECACVSVH GANRLGANS 
      LLETVIFGR AVAHDIGEC NKPGDDIPQ AEDWMGASA 
      IDNLEKLKR QNGNRTVGD LRLELQKTM QKFAGVFRD 
      QKLLEEGCL KVAELYKEI DNIKVNDDS MIWNSDLLE 
      ALELQNLFI NAIQTIRAM EMRKESRGA HARDDYKVR 
      IDEYDYAKP VEGQSKKSF EQHWRKHTL SWLEIETGK 
      VCFTFRPVN DKTLDEDEC PHVPPIIRA Y
    CDS sequence
    • >TC007374 RA
      atgttgaaa ctttcaaaa ctcttcaac ccgatatcg 
      aaacacttg cgaaatttg caaccacga ctatgcttt 
      tcttcagat agtgaagtt ccagtgttt gaccacttt 
      tacgactgt ttagtgatc ggtgctggc ggggccggc 
      cttcgggcc gcttacggc ttaacagcc aaaggtttt 
      aacaccgcc gttgtgaca aaaattttc ccgactcgc 
      tcccacacc gtctcggcc caaggcggc ataaacgca 
      gccctaggc aattatgag cccgacaag tggtactgg 
      cattttttc gacacggtc aagggctcc gactggctc 
      ggagaccaa gacgccatt cactacatg accaaaaat 
      gccccaagt gcaattcta gaattggaa aacgtggga 
      atgcccttt tcgcgattc aaggacggc agaatttat 
      cagagggct ttcgggggc cagtctttg gaatgcggg 
      aaagggggc caggcccat aggacgtgt gcggccgct 
      gacagtacg ggccagttc atgctgcac acgttgtat 
      ggacaagcg gtcaagtat aattgtaaa tttttcgtc 
      gaatatttc gccttggat ttgataatg gatgacgag 
      cggtgttgt ggggcgatc acttgggat ttgagcagt 
      gggactttc caccggttt tttgcaaga aatactgtt 
      atagcgaca ggggggtac gaaagggcg tacttttcc 
      tgcacaact gcgcacact tccaccggg gacgggaca 
      gccatggtg tcccgagcg gggctgccc ttgcaggac 
      ctggagttc gtccagttc catcccaca ggaatttac 
      ggtgttggg gtgcttgtg accgagggc gcaaggggc 
      gaaggtggc tacataacc aacagcaaa ggggagcgt 
      ttcatggag cgatacgcc cctcatgcc aaggattta 
      gcctccaga gacgtagtc tccagaagt tgcgccatg 
      gaaatcctg gaaggtcga ggagtaggt cccgaaaaa 
      gaccacgtc catttggaa ctaatgcac ttgccgcat 
      gaattaatc agaaaacaa ttaccggga atttcaaac 
      ttagtcgcc ggttttgtt ggccgcgat gtcacaaaa 
      gaaccgatt ccaattgta cctacagtt cactataac 
      atgggtggg atccctacg aattataag ggtcaagtc 
      atcacccaa tcgccggaa aaatgcgag cagttggtc 
      acaggactt tacgcgtgc ggcgaatgt gcttgcgtt 
      tcagtccac ggagcgaac agacttggc gctaattcg 
      ttactcgaa actgttatt tttggacgt gctgttgcc 
      catgacatt ggcgagtgt aacaaaccg ggtgacgac 
      atcccacaa gctgaagat tggatgggt gcaagcgct 
      atcgataat ttagaaaaa ctgaaacgg caaaatggc 
      aatcgtacc gtcggcgat ttgcgtcta gagttacaa 
      aaaacgatg caaaaattc gccggagtg tttcgcgat 
      caaaagtta ctggaggaa ggttgtttg aaagtcgcc 
      gaattatat aaggaaata gataacata aaagttaac 
      gacgactca atgatctgg aattcagat cttctcgaa 
      gctttggaa ttacaaaat ttgtttatt aatgccatt 
      caaacgata cgtgccatg gagatgagg aaggaatcg 
      cggggcgcg cacgcgagg gacgattat aaggtaaga 
      atagacgag tacgattac gcaaaaccg gtcgaaggg 
      cagtcgaaa aagtcgttt gagcagcac tggcggaag 
      catacgtta agctggttg gaaattgag accggaaaa 
      gtttgtttc acgttccgt cccgtgaac gacaagacg 
      ctagacgag gacgagtgc ccccacgtg cctccgata 
      atcagggcg tactga
    mRNA sequence
    • >TC007374 RA
      acatttgaa aaaaaaaat gttgaaact ttcaaaact 
      cttcaaccc gatatcgaa acacttgcg aaatttgca 
      accacgact atgcttttc ttcagatag tgaagttcc 
      agtgtttga ccactttta cgactgttt agtgatcgg 
      tgctggcgg ggccggcct tcgggccgc ttacggctt 
      aacagccaa aggttttaa caccgccgt tgtgacaaa 
      aattttccc gactcgctc ccacaccgt ctcggccca 
      aggcggcat aaacgcagc cctaggcaa ttatgagcc 
      cgacaagtg gtactggca ttttttcga cacggtcaa 
      gggctccga ctggctcgg agaccaaga cgccattca 
      ctacatgac caaaaatgc cccaagtgc aattctaga 
      attggaaaa cgtgggaat gcccttttc gcgattcaa 
      ggacggcag aatttatca gagggcttt cgggggcca 
      gtctttgga atgcgggaa agggggcca ggcccatag 
      gacgtgtgc ggccgctga cagtacggg ccagttcat 
      gctgcacac gttgtatgg acaagcggt caagtataa 
      ttgtaaatt tttcgtcga atatttcgc cttggattt 
      gataatgga tgacgagcg gtgttgtgg ggcgatcac 
      ttgggattt gagcagtgg gactttcca ccggttttt 
      tgcaagaaa tactgttat agcgacagg ggggtacga 
      aagggcgta cttttcctg cacaactgc gcacacttc 
      caccgggga cgggacagc catggtgtc ccgagcggg 
      gctgccctt gcaggacct ggagttcgt ccagttcca 
      tcccacagg aatttacgg tgttggggt gcttgtgac 
      cgagggcgc aaggggcga aggtggcta cataaccaa 
      cagcaaagg ggagcgttt catggagcg atacgcccc 
      tcatgccaa ggatttagc ctccagaga cgtagtctc 
      cagaagttg cgccatgga aatcctgga aggtcgagg 
      agtaggtcc cgaaaaaga ccacgtcca tttggaact 
      aatgcactt gccgcatga attaatcag aaaacaatt 
      accgggaat ttcaaactt agtcgccgg ttttgttgg 
      ccgcgatgt cacaaaaga accgattcc aattgtacc 
      tacagttca ctataacat gggtgggat ccctacgaa 
      ttataaggg tcaagtcat cacccaatc gccggaaaa 
      atgcgagca gttggtcac aggacttta cgcgtgcgg 
      cgaatgtgc ttgcgtttc agtccacgg agcgaacag 
      acttggcgc taattcgtt actcgaaac tgttatttt 
      tggacgtgc tgttgccca tgacattgg cgagtgtaa 
      caaaccggg tgacgacat cccacaagc tgaagattg 
      gatgggtgc aagcgctat cgataattt agaaaaact 
      gaaacggca aaatggcaa tcgtaccgt cggcgattt 
      gcgtctaga gttacaaaa aacgatgca aaaattcgc 
      cggagtgtt tcgcgatca aaagttact ggaggaagg 
      ttgtttgaa agtcgccga attatataa ggaaataga 
      taacataaa agttaacga cgactcaat gatctggaa 
      ttcagatct tctcgaagc tttggaatt acaaaattt 
      gtttattaa tgccattca aacgatacg tgccatgga 
      gatgaggaa ggaatcgcg gggcgcgca cgcgaggga 
      cgattataa ggtaagaat agacgagta cgattacgc 
      aaaaccggt cgaagggca gtcgaaaaa gtcgtttga 
      gcagcactg gcggaagca tacgttaag ctggttgga 
      aattgagac cggaaaagt ttgtttcac gttccgtcc 
      cgtgaacga caagacgct agacgagga cgagtgccc 
      ccacgtgcc tccgataat cagggcgta ctgaataag 
      tatcaataa ttatgaaaa aaatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY