Status
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Gene information
General information
TC007344 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5871397..5876636
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007344
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01168

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007344 RA
      MRSVFMVAE KPSLAASLA SILSNGSNS TRKGSNGIC 
      SVHEWNGTF QGAPARLKM TSVCGHVMG VDFISKYNN 
      WDRVDPVEL FSCPIEKKE ATPKFKMPA FLSHEAKGC 
      DYLVLWLDC DKEGENICF EVMNSVARS MNGDVYSDK 
      VTFRARFSA ITDKDIKAA FNNLAHPNE NEARSVDAR 
      QELDLRIGC AFTRFQTKF FQGRYGDLD SSLISYGPC 
      QTPTLGFCV QRHDQIQTF KPEAYWVIK ATIVTKDGN 
      DVHLDWKRV RCFDKEVAN MFLQQIKDQ KHAKVLNVS 
      SKEKAKSRP VALNTVELM RVASSGLGM GPHHAMQIA 
      EKLYTQGYI SYPRTETTR YPENFDLVG VLRQQQNST 
      EWGDEVREI LSRGVNRPK QGHDAGDHP PITPMKSAT 
      RNEFDSDSW KLYDYITRH FIGTLARDC KYLSTTATI 
      AINEEIFTV TGKTLLDPG FTSVMTWQA LDKNEIVPN 
      LTENESVPI KDMRLTEHQ TSPPDYLTE AELITLMET 
      HGIGTDASI PVHINNICQ RNYVTVTAG RKLKPTTLG 
      IVLVHGYQK IDAELVLPT MRSAVEEQL NLIALGKAN 
      FQAVLRHTV EIFKLKFQY FVKNIDGMD QLFEVSFSP 
      LSASGKAHS RCGKCRRYM KYIQAKPPR LHCPQCDET 
      YSLPQNGTI KLFKELKCP LDDFELLYW TMGNKGKSF 
      VFCPYCYNH PPFKDMKKG MGCNSCSHP TCPYSFNSN 
      GVCSCVECD SGVLVLDPS SGPKWKLGC TRCDTILNL 
      FDDAQKLTV LSEVCDCGA QLINIEYKP EKSKLPNGV 
      AEMKGCVFC TTEFGRLVD KPKTAVSSR PRPFRGGKA 
      PSRGRGRGR PKPKDKMAQ LAAYFV
    CDS sequence
    • >TC007344 RA
      atgagaagt gtttttatg gtggctgaa aagccctca 
      ctggcggcg tccctagcg agcatattg agcaacggc 
      tccaactca actcgcaaa ggctcaaac ggtatctgt 
      tccgtgcac gaatggaac ggtaccttt caaggagcc 
      ccagcgagg ctcaaaatg acgtcagtt tgcgggcac 
      gtcatgggg gtcgatttt ataagcaaa tacaataac 
      tgggacagg gttgatcca gttgagctt ttttcgtgt 
      cctattgag aaaaaggaa gccactcca aagtttaaa 
      atgccggct tttttgagc catgaggcc aaagggtgc 
      gattatttg gtcttgtgg cttgattgc gataaagag 
      ggcgaaaat atttgcttc gaagtcatg aattcggtg 
      gcgaggagc atgaacgga gatgtttat tcggacaag 
      gtgacgttt agggcgcgc ttttcggcg attactgat 
      aaggacatc aaagcggcg ttcaataat ttagcccac 
      cctaatgag aacgaagcc aggagtgtt gacgcccgc 
      caagagctg gatttacga atcggttgc gcattcact 
      cgcttccag acaaaattt tttcaaggc cgttatggc 
      gatttagac tcgtcttta atttcctac ggcccttgt 
      cagacccca actttgggt ttttgtgtc caaagacac 
      gaccaaatc caaacattt aaacccgaa gcttactgg 
      gtcatcaaa gccactatt gtgacaaaa gacggcaac 
      gatgtccat ttggattgg aaacgcgtc cgttgtttt 
      gataaagaa gtcgcaaac atgttttta caacaaatc 
      aaagaccag aaacacgca aaagtactg aacgtttcg 
      tcaaaagag aaggcgaaa agccgccca gtggcccta 
      aacaccgtg gagttgatg cgtgtggcg agctccggc 
      ctgggcatg ggcccccat catgccatg caaatcgcc 
      gaaaagttg tacactcaa gggtacata agttacccc 
      cggactgaa acaactcgc tacccggaa aatttcgat 
      ttggttggc gtgcttcgc cagcagcaa aacagcacg 
      gaatggggg gacgaagtc cgcgaaatc ttgtctcgg 
      ggggttaac cggcccaag cagggccac gatgccggg 
      gaccacccc ccgataacc cccatgaaa tcagccact 
      cggaacgaa ttcgattcc gattcgtgg aaattgtac 
      gattacatt acgcgtcat ttcatcggt actttggcg 
      cgggattgc aaatatttg agtaccact gcaactatt 
      gccataaac gaggagatt tttaccgtg actgggaaa 
      actctcctc gatccgggt ttcacgagt gtgatgact 
      tggcaagcg ttggataag aacgaaatt gttccgaat 
      ttgaccgaa aacgagtcg gtacctatc aaagatatg 
      cgcttgacg gagcatcag acttcacct ccggattat 
      ttaaccgag gctgaattg attactttg atggaaacg 
      cacggaatt ggcactgat gcctctatt ccggtgcat 
      attaacaat atttgtcaa agaaattac gtgactgtg 
      accgcggga cggaaatta aaaccgacc actttaggc 
      atcgtcttg gtacacggg taccaaaag attgacgct 
      gagttggtt ttgccgacc atgcggtcg gctgttgag 
      gaacagttg aatctcatt gctttaggt aaagctaat 
      tttcaagcc gttcttagg cacacggtt gagatattt 
      aaattgaaa tttcaatac tttgtgaaa aatattgac 
      ggaatggat cagctattt gaggtttcc ttctcgccc 
      ttaagtgcg tcaggaaag gcacattcg agatgcggc 
      aaatgtcgc agatacatg aagtacatt caagccaaa 
      cccccgcga ctgcattgc ccacaatgc gacgagact 
      tacagtttg ccccaaaac gggacaata aaactattc 
      aaagaactc aaatgccct ctcgacgac ttcgaactg 
      ttgtactgg acaatgggc aacaaaggc aaaagtttc 
      gttttctgc ccttattgt tacaaccat ccacctttc 
      aaagacatg aaaaaaggc atgggctgc aactcgtgt 
      tcacacccg acctgccct tatagcttc aacagcaac 
      ggcgtttgc agctgcgtt gagtgtgat tctggcgtt 
      ttagtcctt gacccatcg tcgggccct aaatggaaa 
      ctcggttgt acccggtgt gacaccatc ttaaatctc 
      ttcgatgac gcgcaaaaa ttgaccgtg ttgtcagag 
      gtgtgcgac tgtggggcc cagctaatc aacattgag 
      tataaacca gaaaaaagc aagttgccg aatggagtt 
      gcggagatg aaaggctgc gttttttgc actactgag 
      tttggccga cttgttgat aagcccaag actgcggtc 
      agctcgagg cctcggccg tttaggggt gggaaggcc 
      cccagtaga ggcaggggc agagggcgg cccaaacct 
      aaggataag atggcccaa cttgccgcc tattttgta 
      taa
    mRNA sequence
    • >TC007344 RA
      ttaaacatt taaatatga gaagtgttt ttatggtgg 
      ctgaaaagc cctcactgg cggcgtccc tagcgagca 
      tattgagca acggctcca actcaactc gcaaaggct 
      caaacggta tctgttccg tgcacgaat ggaacggta 
      cctttcaag gagccccag cgaggctca aaatgacgt 
      cagtttgcg ggcacgtca tgggggtcg attttataa 
      gcaaataca ataactggg acagggttg atccagttg 
      agctttttt cgtgtccta ttgagaaaa aggaagcca 
      ctccaaagt ttaaaatgc cggcttttt tgagccatg 
      aggccaaag ggtgcgatt atttggtct tgtggcttg 
      attgcgata aagagggcg aaaatattt gcttcgaag 
      tcatgaatt cggtggcga ggagcatga acggagatg 
      tttattcgg acaaggtga cgtttaggg cgcgctttt 
      cggcgatta ctgataagg acatcaaag cggcgttca 
      ataatttag cccacccta atgagaacg aagccagga 
      gtgttgacg cccgccaag agctggatt tacgaatcg 
      gttgcgcat tcactcgct tccagacaa aattttttc 
      aaggccgtt atggcgatt tagactcgt ctttaattt 
      cctacggcc cttgtcaga ccccaactt tgggttttt 
      gtgtccaaa gacacgacc aaatccaaa catttaaac 
      ccgaagctt actgggtca tcaaagcca ctattgtga 
      caaaagacg gcaacgatg tccatttgg attggaaac 
      gcgtccgtt gttttgata aagaagtcg caaacatgt 
      ttttacaac aaatcaaag accagaaac acgcaaaag 
      tactgaacg tttcgtcaa aagagaagg cgaaaagcc 
      gcccagtgg ccctaaaca ccgtggagt tgatgcgtg 
      tggcgagct ccggcctgg gcatgggcc cccatcatg 
      ccatgcaaa tcgccgaaa agttgtaca ctcaagggt 
      acataagtt acccccgga ctgaaacaa ctcgctacc 
      cggaaaatt tcgatttgg ttggcgtgc ttcgccagc 
      agcaaaaca gcacggaat ggggggacg aagtccgcg 
      aaatcttgt ctcgggggg ttaaccggc ccaagcagg 
      gccacgatg ccggggacc accccccga taaccccca 
      tgaaatcag ccactcgga acgaattcg attccgatt 
      cgtggaaat tgtacgatt acattacgc gtcatttca 
      tcggtactt tggcgcggg attgcaaat atttgagta 
      ccactgcaa ctattgcca taaacgagg agattttta 
      ccgtgactg ggaaaactc tcctcgatc cgggtttca 
      cgagtgtga tgacttggc aagcgttgg ataagaacg 
      aaattgttc cgaatttga ccgaaaacg agtcggtac 
      ctatcaaag atatgcgct tgacggagc atcagactt 
      cacctccgg attatttaa ccgaggctg aattgatta 
      ctttgatgg aaacgcacg gaattggca ctgatgcct 
      ctattccgg tgcatatta acaatattt gtcaaagaa 
      attacgtga ctgtgaccg cgggacgga aattaaaac 
      cgaccactt taggcatcg tcttggtac acgggtacc 
      aaaagattg acgctgagt tggttttgc cgaccatgc 
      ggtcggctg ttgaggaac agttgaatc tcattgctt 
      taggtaaag ctaattttc aagccgttc ttaggcaca 
      cggttgaga tatttaaat tgaaatttc aatactttg 
      tgaaaaata ttgacggaa tggatcagc tatttgagg 
      tttccttct cgcccttaa gtgcgtcag gaaaggcac 
      attcgagat gcggcaaat gtcgcagat acatgaagt 
      acattcaag ccaaacccc cgcgactgc attgcccac 
      aatgcgacg agacttaca gtttgcccc aaaacggga 
      caataaaac tattcaaag aactcaaat gccctctcg 
      acgacttcg aactgttgt actggacaa tgggcaaca 
      aaggcaaaa gtttcgttt tctgccctt attgttaca 
      accatccac ctttcaaag acatgaaaa aaggcatgg 
      gctgcaact cgtgttcac acccgacct gcccttata 
      gcttcaaca gcaacggcg tttgcagct gcgttgagt 
      gtgattctg gcgttttag tccttgacc catcgtcgg 
      gccctaaat ggaaactcg gttgtaccc ggtgtgaca 
      ccatcttaa atctcttcg atgacgcgc aaaaattga 
      ccgtgttgt cagaggtgt gcgactgtg gggcccagc 
      taatcaaca ttgagtata aaccagaaa aaagcaagt 
      tgccgaatg gagttgcgg agatgaaag gctgcgttt 
      tttgcacta ctgagtttg gccgacttg ttgataagc 
      ccaagactg cggtcagct cgaggcctc ggccgttta 
      ggggtggga aggccccca gtagaggca ggggcagag 
      ggcggccca aacctaagg ataagatgg cccaacttg 
      ccgcctatt ttgtataat tcgttaaaa aataaacga 
      ttttggcgc aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY