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Gene information
General information
TC007336 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5989301..5994926
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007336
    iBeetle screen iB_01167

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection



    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    wings slightly shortened -

    wings shape irregular - Number of animals showing the phenotype: 8
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007336 RA
      MVLAEKNSE NARNGRSRG PSPNGHVQD STSEDESSV 
      PVDKIRVGR DYQAICPEL QPESQRKPE LLADRALLV 
      WSPTESIPE SKLEDYIVL AKDKYGYNG EQALGMLFW 
      HKHDLDRAV LDLANFTPF PDEWSVEDK VLFEQAFQF 
      HGKSFHRIR QMLPDKSIA SLVKYYYSW KKTRSRTSL 
      MDKQARKLN TPKEEGGAS EAGSEGGSN EESDHDDKI 
      QQNKQNISS LKRKHADSI EDLRPTNDN SSRINARWT 
      NEELLLAVQ GVRKYGKDF KSIAEVLGN KTEHHVRTF 
      FVNYRKRYN LDNVLKDWE KDNGPLPED NGDVKVELD 
      DSNNDNDVI CISPTPKKE MKNGKVTQA SK
    • >TC007336 RB
      MVLAEKNSE NARNGRSRG PSPNGHVQD STSEDESSV 
      PVDKIRVGR DYQAICPEL QPESQRKPE LLADRALLV 
      WSPTESIPE SKLEDYIVL AKDKYGYNG EQALGMLFW 
      HKHDLDRAV LDLANFTPF PDEWSVEDK VLFEQAFQF 
      HGKSFHRIR QMLPDKSIA SLVKYYYSW KKTRSRTSL 
      MDKQARKLN TPKEEGGAS EAGSEGGSN EESDHDDKG 
      EGGACSNCG VACTVTQVT SKGSLCNSC YQHWRRTGL 
      LRPTSGPTG GKRGSPLGG RHKRKPPRG MYINHDDLA 
      AMASGNAGV LMLKAMDRE LDSLQRQIQ QNKQNISSL 
      KRKHADSIE DLRPTNDNS SRINARWTN EELLLAVQG 
      VRKYGKDFK SIAEVLGNK TEHHVRTFF VNYRKRYNL 
      DNVLKDWEK DNGPLPEDN GDVKVELDD SNNDNDVIC 
      ISPTPKKEM KNGKVTQAS K
    CDS sequence
    • >TC007336 RA
      atggttcta gccgaaaaa aacagtgaa aatgcccga 
      aacggtcgg agtcgagga ccgagtccc aatggtcac 
      gttcaagac tccacaagc gaggatgaa agctcagtt 
      ccagtagat aagattcga gttgggcga gactaccag 
      gccatttgc cccgaactt cagccggaa tcgcagcga 
      aaacccgaa ttattggcc gatcgagcg ctacttgta 
      tggtcacca actgagtcc atccccgag tcaaagctg 
      gaggattac atagtttta gccaaagac aagtacggt 
      tacaacggg gagcaggcg cttgggatg ctcttttgg 
      cacaagcat gatttagac cgggcggtg ctggacttg 
      gcgaacttc acccccttc cccgacgag tggtcggtc 
      gaagacaag gtgctgttc gagcaggcg tttcagttc 
      cacggcaaa agctttcat aggataaga caaatgttg 
      ccagataaa agtattgca agtttagtc aagtattac 
      tacagttgg aaaaagaca aggtctagg accagcctc 
      atggacaag caggcacgt aagttgaac acccccaag 
      gaggaaggg ggcgcttca gaggccggc tcagaggga 
      ggatccaac gaggaatca gaccatgac gataagatc 
      cagcagaac aagcagaat ataagttcc ttaaagcgc 
      aagcatgca gactccatt gaagatctc cgtccaaca 
      aatgacaat tcgtctcgt attaacgcc cgctggaca 
      aacgaggaa ttgctcttg gctgtccaa ggcgtgcgt 
      aagtacggc aaagacttt aaaagcatc gccgaagta 
      ttgggtaac aaaactgaa caccatgtg cgtacgttt 
      ttcgttaat taccgtaaa agatataat ttagacaat 
      gttctaaaa gactgggaa aaagataat gggcctttg 
      cctgaagat aacggcgac gttaaagtc gaattggac 
      gattccaac aatgataac gatgttatt tgtatatca 
      ccgacccca aaaaaagaa atgaaaaac gggaaagtc 
      actcaagct agtaaatga
    • >TC007336 RB
      atggttcta gccgaaaaa aacagtgaa aatgcccga 
      aacggtcgg agtcgagga ccgagtccc aatggtcac 
      gttcaagac tccacaagc gaggatgaa agctcagtt 
      ccagtagat aagattcga gttgggcga gactaccag 
      gccatttgc cccgaactt cagccggaa tcgcagcga 
      aaacccgaa ttattggcc gatcgagcg ctacttgta 
      tggtcacca actgagtcc atccccgag tcaaagctg 
      gaggattac atagtttta gccaaagac aagtacggt 
      tacaacggg gagcaggcg cttgggatg ctcttttgg 
      cacaagcat gatttagac cgggcggtg ctggacttg 
      gcgaacttc acccccttc cccgacgag tggtcggtc 
      gaagacaag gtgctgttc gagcaggcg tttcagttc 
      cacggcaaa agctttcat aggataaga caaatgttg 
      ccagataaa agtattgca agtttagtc aagtattac 
      tacagttgg aaaaagaca aggtctagg accagcctc 
      atggacaag caggcacgt aagttgaac acccccaag 
      gaggaaggg ggcgcttca gaggccggc tcagaggga 
      ggatccaac gaggaatca gaccatgac gataagggt 
      gagggtggc gcgtgctct aactgcgga gtcgcctgt 
      accgtcact caagtgacg tccaagggg tccctctgt 
      aattcctgc taccaacac tggagacga acgggattg 
      ttgcggccc acatcgggg cccacgggt ggcaagcgc 
      gggtcccca ctgggcgga cgccacaag cgcaagccc 
      cctcgcggc atgtacatc aaccacgac gatctggct 
      gcgatggcg tcaggcaac gcgggtgtg ctcatgctt 
      aaggcgatg gatcgagaa ttagattcc ctccaaaga 
      caaatccag cagaacaag cagaatata agttcctta 
      aagcgcaag catgcagac tccattgaa gatctccgt 
      ccaacaaat gacaattcg tctcgtatt aacgcccgc 
      tggacaaac gaggaattg ctcttggct gtccaaggc 
      gtgcgtaag tacggcaaa gactttaaa agcatcgcc 
      gaagtattg ggtaacaaa actgaacac catgtgcgt 
      acgtttttc gttaattac cgtaaaaga tataattta 
      gacaatgtt ctaaaagac tgggaaaaa gataatggg 
      cctttgcct gaagataac ggcgacgtt aaagtcgaa 
      ttggacgat tccaacaat gataacgat gttatttgt 
      atatcaccg accccaaaa aaagaaatg aaaaacggg 
      aaagtcact caagctagt aaatga
    mRNA sequence
    • >TC007336 RA
      gtggtcgtt gaaaaacgc taaaaatta cgatgtatt 
      gtggaaaaa atgcgtttg accgtagcg ccgtggcgg 
      tttttggaa ataagccgt ggtaaacta cggttctcc 
      gatgacgat tttctagtc gctttgtag tttgagtaa 
      gctgtggtg ttgctgaat catagactc ggttttttc 
      ttaagatta attttctat tatttatgt gaagctcta 
      gttaggagg agcgaagat ggttctagc cgaaaaaaa 
      cagtgaaaa tgcccgaaa cggtcggag tcgaggacc 
      gagtcccaa tggtcacgt tcaagactc cacaagcga 
      ggatgaaag ctcagttcc agtagataa gattcgagt 
      tgggcgaga ctaccaggc catttgccc cgaacttca 
      gccggaatc gcagcgaaa acccgaatt attggccga 
      tcgagcgct acttgtatg gtcaccaac tgagtccat 
      ccccgagtc aaagctgga ggattacat agttttagc 
      caaagacaa gtacggtta caacgggga gcaggcgct 
      tgggatgct cttttggca caagcatga tttagaccg 
      ggcggtgct ggacttggc gaacttcac ccccttccc 
      cgacgagtg gtcggtcga agacaaggt gctgttcga 
      gcaggcgtt tcagttcca cggcaaaag ctttcatag 
      gataagaca aatgttgcc agataaaag tattgcaag 
      tttagtcaa gtattacta cagttggaa aaagacaag 
      gtctaggac cagcctcat ggacaagca ggcacgtaa 
      gttgaacac ccccaagga ggaaggggg cgcttcaga 
      ggccggctc agagggagg atccaacga ggaatcaga 
      ccatgacga taagatcca gcagaacaa gcagaatat 
      aagttcctt aaagcgcaa gcatgcaga ctccattga 
      agatctccg tccaacaaa tgacaattc gtctcgtat 
      taacgcccg ctggacaaa cgaggaatt gctcttggc 
      tgtccaagg cgtgcgtaa gtacggcaa agactttaa 
      aagcatcgc cgaagtatt gggtaacaa aactgaaca 
      ccatgtgcg tacgttttt cgttaatta ccgtaaaag 
      atataattt agacaatgt tctaaaaga ctgggaaaa 
      agataatgg gcctttgcc tgaagataa cggcgacgt 
      taaagtcga attggacga ttccaacaa tgataacga 
      tgttatttg tatatcacc gaccccaaa aaaagaaat 
      gaaaaacgg gaaagtcac tcaagctag taaatgata 
      actttgtac tcgtttctg atttgttgg ataggttcg 
      ttttttatt catatcgac gtgtattat gtattgttt 
      ttaataaaa taatatatc caac
    • >TC007336 RB
      gtggtcgtt gaaaaacgc taaaaatta cgatgtatt 
      gtggaaaaa atgcgtttg accgtagcg ccgtggcgg 
      tttttggaa ataagccgt ggtaaacta cggttctcc 
      gatgacgat tttctagtc gctttgtag tttgagtaa 
      gctgtggtg ttgctgaat catagactc ggttttttc 
      ttaagatta attttctat tatttatgt gaagctcta 
      gttaggagg agcgaagat ggttctagc cgaaaaaaa 
      cagtgaaaa tgcccgaaa cggtcggag tcgaggacc 
      gagtcccaa tggtcacgt tcaagactc cacaagcga 
      ggatgaaag ctcagttcc agtagataa gattcgagt 
      tgggcgaga ctaccaggc catttgccc cgaacttca 
      gccggaatc gcagcgaaa acccgaatt attggccga 
      tcgagcgct acttgtatg gtcaccaac tgagtccat 
      ccccgagtc aaagctgga ggattacat agttttagc 
      caaagacaa gtacggtta caacgggga gcaggcgct 
      tgggatgct cttttggca caagcatga tttagaccg 
      ggcggtgct ggacttggc gaacttcac ccccttccc 
      cgacgagtg gtcggtcga agacaaggt gctgttcga 
      gcaggcgtt tcagttcca cggcaaaag ctttcatag 
      gataagaca aatgttgcc agataaaag tattgcaag 
      tttagtcaa gtattacta cagttggaa aaagacaag 
      gtctaggac cagcctcat ggacaagca ggcacgtaa 
      gttgaacac ccccaagga ggaaggggg cgcttcaga 
      ggccggctc agagggagg atccaacga ggaatcaga 
      ccatgacga taagggtga gggtggcgc gtgctctaa 
      ctgcggagt cgcctgtac cgtcactca agtgacgtc 
      caaggggtc cctctgtaa ttcctgcta ccaacactg 
      gagacgaac gggattgtt gcggcccac atcggggcc 
      cacgggtgg caagcgcgg gtccccact gggcggacg 
      ccacaagcg caagccccc tcgcggcat gtacatcaa 
      ccacgacga tctggctgc gatggcgtc aggcaacgc 
      gggtgtgct catgcttaa ggcgatgga tcgagaatt 
      agattccct ccaaagaca aatccagca gaacaagca 
      gaatataag ttccttaaa gcgcaagca tgcagactc 
      cattgaaga tctccgtcc aacaaatga caattcgtc 
      tcgtattaa cgcccgctg gacaaacga ggaattgct 
      cttggctgt ccaaggcgt gcgtaagta cggcaaaga 
      ctttaaaag catcgccga agtattggg taacaaaac 
      tgaacacca tgtgcgtac gtttttcgt taattaccg 
      taaaagata taatttaga caatgttct aaaagactg 
      ggaaaaaga taatgggcc tttgcctga agataacgg 
      cgacgttaa agtcgaatt ggacgattc caacaatga 
      taacgatgt tatttgtat atcaccgac cccaaaaaa 
      agaaatgaa aaacgggaa agtcactca agctagtaa 
      atgataact ttgtactcg tttctgatt tgttggata 
      ggttcgttt tttattcat atcgacgtg tattatgta 
      ttgttttta ataaaataa tatatccaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY