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Gene information
General information
TC007315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6221792..6223586
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007315
    iBeetle screen iB_01165

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007315 RA
      MTFMTESLI FDIVTIIST IVVLTIAFW KWKLSYWDR 
      VGLPTLSPI LFFGDTKDV LLGRLTFGE QFQELYKKL 
      KAKGLKHGG VYLGTRPFY LPTDPEIIK HIWQKDFQH 
      FVNHGNYFD EEADPLSGH LFNLEDAKW KNMRVKLTP 
      TFTSRKMKM MFQTLADCT HGLKDIMDE GASSGSPVD 
      IKEILGRFT TDIIGSVAF GLECNSLKD PNTLFRMYG 
      KKIFELSTG RRLHLFLQF ILPERVLHA MKFKVTPSD 
      VETFFMKVI RDTVGYREK NNVYRKDFL HMLIQLKNR 
      GTVTDDEKI TEDNGKTGE KALTMNELA AQSFVFFAA 
      GFETSSTTV TFALLELAL NQDIQQKLR EEINTCLAK 
      SNGELTYQA IMEMTYMDK VLQETLRKY PPVPFLTRR 
      CTKDYTIPE TSIKLRKGD HVGISVVGI QNDPEYYPD 
      PEKFVPERF NEENKNSRH PFTWMPFGE GPRICIGLR 
      FGMLQSKVG LAALLKNYK ITLNSKTKM PIEMEKSSF 
      ITSVNGGVW LNVEKLS
    CDS sequence
    • >TC007315 RA
      atgactttc atgacagaa tccctaatt tttgatatc 
      gttaccatt atttcaaca attgtggta ctgaccatc 
      gctttttgg aaatggaaa ctgtcttat tgggaccgt 
      gtaggacta ccaacttta tcaccgatt ctatttttt 
      ggcgataca aaagatgta ctattggga cgattaact 
      tttggtgaa cagttccaa gaactgtac aaaaaactt 
      aaagcaaag ggactcaaa catggaggt gtttacctt 
      ggaactcga ccattttac cttccgacc gaccctgaa 
      attatcaag catatctgg caaaaagat ttccaacat 
      ttcgttaac catggcaat tatttcgac gaagaggcc 
      gatccatta agtggacat ttgttcaat ttagaagac 
      gcgaagtgg aaaaatatg agagtgaaa ctaacaccg 
      acttttact tcgagaaaa atgaagatg atgttccag 
      actttggcc gattgtacg cacggcctt aaggacatt 
      atggatgaa ggtgcatca agtggtagt cctgtcgat 
      attaaggaa attctagga cgtttcaca actgatatt 
      attggatca gtggctttc ggtcttgaa tgtaacagt 
      ctcaaagac ccaaacact ttattcaga atgtacgga 
      aaaaagatt tttgaacta agtacagga cgcagactt 
      catctcttt ctgcagttc atcttacca gagcgtgtt 
      ctacatgca atgaaattc aaagtaaca ccatctgat 
      gttgagacg tttttcatg aaagttatt cgagacacg 
      gtcggttat agagaaaaa aataacgtg tatagaaag 
      gacttcttg catatgctg atacaactg aaaaatcgt 
      ggcacagtg acagatgat gaaaaaata acggaagac 
      aatggtaaa actggagaa aaagctttg acaatgaat 
      gaacttgca gctcagtcg tttgtattt ttcgccgct 
      gggtttgaa acgtcttca acaactgtg acttttgct 
      ttgttagaa ttagcgctt aatcaagac attcaacaa 
      aaactcaga gaagaaata aacacatgc cttgccaaa 
      tctaacggc gaattaact taccaagct attatggaa 
      atgacctac atggacaag gttttgcaa gaaactcta 
      agaaagtat cctcctgta ccatttcta acccgccgt 
      tgtacaaaa gactacact attcctgag acttccatc 
      aaactccga aaaggagac catgttggt atctccgtt 
      gtaggcata caaaatgat cctgagtat tatcctgat 
      cctgaaaaa ttcgtcccg gaacgattc aatgaagaa 
      aataaaaat tcgagacat cctttcacg tggatgcct 
      ttcggtgaa ggaccaaga atttgtatt ggtttacgc 
      tttggtatg ttacaaagt aaggtcgga cttgccgct 
      cttttgaaa aattacaaa ataacgtta aattcgaag 
      acgaaaatg cccatagaa atggagaaa tcgtctttc 
      attacatcg gtaaacgga ggtgtgtgg ttgaacgtt 
      gagaagctg agttaa
    mRNA sequence
    • >TC007315 RA
      cactcgcaa attcaaggt gaatgagtt agcggagtg 
      ttttttttt gtgcaaagt tataaatat ttctgcaac 
      cagaatgac tttcatgac agaatccct aatttttga 
      tatcgttac cattatttc aacaattgt ggtactgac 
      catcgcttt ttggaaatg gaaactgtc ttattggga 
      ccgtgtagg actaccaac tttatcacc gattctatt 
      ttttggcga tacaaaaga tgtactatt gggacgatt 
      aacttttgg tgaacagtt ccaagaact gtacaaaaa 
      acttaaagc aaagggact caaacatgg aggtgttta 
      ccttggaac tcgaccatt ttaccttcc gaccgaccc 
      tgaaattat caagcatat ctggcaaaa agatttcca 
      acatttcgt taaccatgg caattattt cgacgaaga 
      ggccgatcc attaagtgg acatttgtt caatttaga 
      agacgcgaa gtggaaaaa tatgagagt gaaactaac 
      accgacttt tacttcgag aaaaatgaa gatgatgtt 
      ccagacttt ggccgattg tacgcacgg ccttaagga 
      cattatgga tgaaggtgc atcaagtgg tagtcctgt 
      cgatattaa ggaaattct aggacgttt cacaactga 
      tattattgg atcagtggc tttcggtct tgaatgtaa 
      cagtctcaa agacccaaa cactttatt cagaatgta 
      cggaaaaaa gatttttga actaagtac aggacgcag 
      acttcatct ctttctgca gttcatctt accagagcg 
      tgttctaca tgcaatgaa attcaaagt aacaccatc 
      tgatgttga gacgttttt catgaaagt tattcgaga 
      cacggtcgg ttatagaga aaaaaataa cgtgtatag 
      aaaggactt cttgcatat gctgataca actgaaaaa 
      tcgtggcac agtgacaga tgatgaaaa aataacgga 
      agacaatgg taaaactgg agaaaaagc tttgacaat 
      gaatgaact tgcagctca gtcgtttgt atttttcgc 
      cgctgggtt tgaaacgtc ttcaacaac tgtgacttt 
      tgctttgtt agaattagc gcttaatca agacattca 
      acaaaaact cagagaaga aataaacac atgccttgc 
      caaatctaa cggcgaatt aacttacca agctattat 
      ggaaatgac ctacatgga caaggtttt gcaagaaac 
      tctaagaaa gtatcctcc tgtaccatt tctaacccg 
      ccgttgtac aaaagacta cactattcc tgagacttc 
      catcaaact ccgaaaagg agaccatgt tggtatctc 
      cgttgtagg catacaaaa tgatcctga gtattatcc 
      tgatcctga aaaattcgt cccggaacg attcaatga 
      agaaaataa aaattcgag acatccttt cacgtggat 
      gcctttcgg tgaaggacc aagaatttg tattggttt 
      acgctttgg tatgttaca aagtaaggt cggacttgc 
      cgctctttt gaaaaatta caaaataac gttaaattc 
      gaagacgaa aatgcccat agaaatgga gaaatcgtc 
      tttcattac atcggtaaa cggaggtgt gtggttgaa 
      cgttgagaa gctgagtta aaaaattat taataccaa 
      agaagaaat atacaatac atagttatt taattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY