Status
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Gene information
General information
TC007297 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6424353..6433057
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007297
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01162

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007297 RA
      MTDSNKKEK FPSTFTVDN FSSTTKLAD HEYKIPVGS 
      VDTLAEKEY SPYEHRNVE HPNTFSGAL MHLLKSSLG 
      TGILAIPSA VAAAGIVIG VIGTVLTGI LCTHTIHLL 
      IFASQEICK KAKVPMLGF AETAHAVFK YGPKPVQPL 
      ANFARIFVD VALLLTYYA GNAVYIVFI CGSVQDLVN 
      YHYASVSHW PIQYYMLML LVPLTLCCQ VRQLKHLVP 
      FSIIANVTM VTAFLITLY YMFSGISSI KIEERKLFK 
      DVSLIPLFF STVLFAMEG IGTMLPIEN SMIKPQFIG 
      CPGVLNVAM SFVVTLYTI IGLFGYIRF GDSVKANVI 
      EELPNSDIA AQVAKLCIA IAVFFTFML QFYVPCDIT 
      WRKLARKIP EKHHNVSQI VMRTILVCF VTGIAAAVP 
      KLDAIIGLV GSVFFSTLG LFIPVVIDI ILNLGENGD 
      FGFMKWRLW KNIFVIVIS WFALFSGSY YAIKGLLND
    CDS sequence
    • >TC007297 RA
      atgacagac agcaacaaa aaagaaaag tttccgtcg 
      acattcact gtcgacaat ttcagctcg acaactaaa 
      cttgccgat catgagtac aaaatcccg gtcgggagt 
      gttgacaca cttgctgag aaggaatac agtccttac 
      gaacacaga aatgtggaa catcccaac acgttttcc 
      ggagcgttg atgcacctt ttgaagagt tcgctagga 
      acgggaatt ttggccata cctagtgca gtggccgct 
      gccgggatt gttattggt gttataggg acggtcctg 
      actggaatt ctctgcacc cacaccatc cacctgctg 
      atattcgca tcgcaagaa atatgcaag aaggccaaa 
      gtcccgatg ctgggcttc gccgaaacg gcccatgcc 
      gtcttcaag tacggcccg aagccggtc cagcccttg 
      gcaaacttc gccaggatt tttgttgac gtcgccctt 
      cttttgact tactatgca ggcaatgcc gtttacatt 
      gtttttatt tgtgggagc gtccaagat cttgtgaat 
      tatcactac gcgtccgtc tcccattgg ccgattcag 
      tattacatg cttatgctt ttggtgcct ttgactttg 
      tgttgccaa gtgcggcag ttgaaacac ttggttcca 
      ttctcaata atcgcaaat gtgacaatg gttactgct 
      ttcctcatc actttgtat tacatgttc tccggaatc 
      agttctatc aaaattgag gaacgaaaa ctgttcaaa 
      gacgtttcc ctaatcccg cttttcttc agcactgtg 
      ttgtttgca atggaggga attggcaca atgctgccg 
      attgaaaac tcaatgatc aaaccgcaa tttatcgga 
      tgtccggga gtcctaaac gttgccatg agctttgtt 
      gtgactctt tacacaatt attggtttg ttcggttac 
      attagattc ggtgattcg gtcaaagcg aacgtcatt 
      gaggaatta ccaaacagc gacatagcg gcccaagtg 
      gccaaactg tgcatagcc atcgcagtt ttcttcacc 
      tttatgttg caattctac gtcccgtgt gacataacg 
      tggagaaaa ctcgcccgg aaaattccg gaaaagcac 
      cacaacgtt tcccaaata gtgatgagg actattttg 
      gtgtgtttt gtaaccgga attgctgca gctgtgccc 
      aaactggac gctatcata ggactggtg ggatcagtg 
      tttttctcc acccttgga cttttcatc cctgtcgtc 
      attgatatt attttgaat ttaggcgaa aacggcgat 
      tttgggttc atgaagtgg cgattatgg aagaacatt 
      tttgttatt gttatttcg tggtttgct ctattttca 
      ggaagttat tacgcgatc aagggacta ctgaacgat 
      tga
    mRNA sequence
    • >TC007297 RA
      tgtaataac aatcaattt attttgtta ttacccact 
      ttattattg tagaagtga ttgtggaaa cgacacgtt 
      tccaataat tgttatcag aaatggaaa ttagataat 
      tgaattttc ctttgataa aaagcaaat aaaattaag 
      taaatctta ttctaactt aaaataatt gtccattta 
      cgttccgtg tgtaaataa ggtgataat aaaaagtct 
      ccactttgt aaataactt cgttatcaa aagagagcc 
      tttaattta ccaaaaacg gcggttaaa gtaacttta 
      cagtaactt tatgaccta gttgcgtct ccgagccat 
      ctaaacttt ttacccgtg ttacccttg catacacgg 
      cgattccgg caattgcaa tgggacttg ctttgaatt 
      ttaatagaa gaaccactt taggaattc gaaggtcag 
      tggcaaaag tttataaga gattacggt taattgttg 
      gctgatgaa tgcaaacgg ccaattttt accaactta 
      actcaccaa agtacaaac agtaacgaa ttaatttct 
      gacgcaagg atcagcgac tcgtgaaaa atgtcattg 
      tggggacct tcgagatca aacaagttt atttctatt 
      ccaagaaac gatcggtaa atttttagc tgcttttcg 
      atgggcgcg aaaatgtga tttgtgtac cacaaggtg 
      attattttg tttaccttt gcagttctc acagttttg 
      aacaataaa attacgggt aattaacgt tggaattct 
      gtggcttat caataagta caatcaata taaatttaa 
      cttcctgaa acgccggta tttaatcat ttttgtcca 
      ataattgga tgagtggcg gttgggtaa aatgtgtaa 
      gaaaacttc acacttgga gtaatcgaa aaatgataa 
      gaaaattgc tacacatgt cgtgaatgt tcgagtagg 
      aatttttta attaaattc cgtgcaaaa agcttattg 
      aaagaggaa attaagctc atattgaaa tctgctgaa 
      agcatagat tattatcga taattagct atcatttaa 
      ttgccttgg cataacttt agcaacagg attcagtat 
      aaattatga tactgacat caacgcatt gtatttaca 
      ttaattacg tgatgtcac atctggata ttgtcgtca 
      catgtgagt cctttcgac gtcaaaaaa gaaagagtc 
      gcgaaaatc tctggatgc tgattagtg gctaattct 
      tcgcacagc aagtttcct gtttatata aaataaggc 
      aaggacatt ggtccgttg ggtatagac agcgtgtag 
      actccacta atatatata ccatcacag gtgctataa 
      tttacttag tatagtcgc gtactgctg ttggatata 
      tacatcatg tgtagtacg cacaaccgt gttagtggg 
      taaattaat aattacaac aactctgat tggtttatt 
      gtatcttac tcagaattt tcagtcacc gaaactaga 
      aactgatag atgtcgacg cggttttcc ccaaatttt 
      cgcagcttt cacttgatc gctaaatcg gatttttag 
      cagaaacca tgacagaca gcaacaaaa aagaaaagt 
      ttccgtcga cattcactg tcgacaatt tcagctcga 
      caactaaac ttgccgatc atgagtaca aaatcccgg 
      tcgggagtg ttgacacac ttgctgaga aggaataca 
      gtccttacg aacacagaa atgtggaac atcccaaca 
      cgttttccg gagcgttga tgcaccttt tgaagagtt 
      cgctaggaa cgggaattt tggccatac ctagtgcag 
      tggccgctg ccgggattg ttattggtg ttataggga 
      cggtcctga ctggaattc tctgcaccc acaccatcc 
      acctgctga tattcgcat cgcaagaaa tatgcaaga 
      aggccaaag tcccgatgc tgggcttcg ccgaaacgg 
      cccatgccg tcttcaagt acggcccga agccggtcc 
      agcccttgg caaacttcg ccaggattt ttgttgacg 
      tcgcccttc ttttgactt actatgcag gcaatgccg 
      tttacattg tttttattt gtgggagcg tccaagatc 
      ttgtgaatt atcactacg cgtccgtct cccattggc 
      cgattcagt attacatgc ttatgcttt tggtgcctt 
      tgactttgt gttgccaag tgcggcagt tgaaacact 
      tggttccat tctcaataa tcgcaaatg tgacaatgg 
      ttactgctt tcctcatca ctttgtatt acatgttct 
      ccggaatca gttctatca aaattgagg aacgaaaac 
      tgttcaaag acgtttccc taatcccgc ttttcttca 
      gcactgtgt tgtttgcaa tggagggaa ttggcacaa 
      tgctgccga ttgaaaact caatgatca aaccgcaat 
      ttatcggat gtccgggag tcctaaacg ttgccatga 
      gctttgttg tgactcttt acacaatta ttggtttgt 
      tcggttaca ttagattcg gtgattcgg tcaaagcga 
      acgtcattg aggaattac caaacagcg acatagcgg 
      cccaagtgg ccaaactgt gcatagcca tcgcagttt 
      tcttcacct ttatgttgc aattctacg tcccgtgtg 
      acataacgt ggagaaaac tcgcccgga aaattccgg 
      aaaagcacc acaacgttt cccaaatag tgatgagga 
      ctattttgg tgtgttttg taaccggaa ttgctgcag 
      ctgtgccca aactggacg ctatcatag gactggtgg 
      gatcagtgt ttttctcca cccttggac ttttcatcc 
      ctgtcgtca ttgatatta ttttgaatt taggcgaaa 
      acggcgatt ttgggttca tgaagtggc gattatgga 
      agaacattt ttgttattg ttatttcgt ggtttgctc 
      tattttcag gaagttatt acgcgatca agggactac 
      tgaacgatt gaagtgaac aaaaattgt gattcgagt 
      aagctttaa agtagggta ggcaaaatt gaccgaatt 
      atttatttg tgattttta ataaaaaat gtgcacaaa 
      aaagtgaag gagttcctt tcaaacatt tgttagtaa 
      acacgcagg tacttaact aaataattg tttttctag 
      tttaaatgt gatttagat taatctcaa ctttgttgt 
      tcgtcgaaa tgtttgcaa taaattgga atttttatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY