General information | |||
TC007295 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 6439389..6449707 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC007295 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01161 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
elytra partially blistered - Number of animals showing the phenotype: 7
mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
ovary without detectable defects - Number of animals showing the phenotype: 3
- >TC007295 RA
MSVMQMQQA KRQHCYLCD LPRMPWAMV HDFSEPVCR GCVNYEGAD RIEIVLETA RQMKRAHGF QEQRSASHK SHRTAASSH EHQNGEVVA TSSRQQPAT HHSGTYSLH HPRSGMLAD YQAQQQPLP PRGTAIPRP QQMETTQEP EALVARPTV RLPTATHLA HHQLQSHHH SANGRPGLP QQGLKRGLS AADDDDHHG LHHANGDMN GAKRMMSVE EHSNAVRPP LTRGESLPA VSLAQPFVV STERTFKQE KHPLRTASF DTATAFKPN VPVSVASAN GSSSGSPLN NRTGSPPDP NAGTTQQTQ QSGGTGQSP MAALMSVAD NLPPGSPRS AGGSPPSSA APRSASRGS QHSPNSTGS SSGRRSSGS RHVSSTTVT STEAGGVMG PNAESMTGS GDSVAPTAP ATTATLKCT LCQERLEDT HFVQCPSVP HHKFCFPCS RESIKRQGA GSEVYCPSG EKCPLANSN VPWAFMQGE IATILGEEY KVKKERET
- >TC007295 RA
atgtcggtc atgcagatg cagcaggcg aaacgtcag cattgctac ttgtgcgac ctgcccagg atgccgtgg gccatggtc catgacttc tccgagccc gtctgtcga gggtgcgtc aactacgaa ggcgccgac aggatcgag atcgtcctg gagacggca cggcagatg aaacgagcc catggcttc caagagcag cgctcagct tcgcataag agccaccgg acggccgcc tccagccat gagcaccag aacggcgaa gtggtggcg acgtccagc cgacaacaa cccgccacg caccattcg ggaacgtac agcttgcac catccgcgt tccgggatg ttggcggac taccaagcc cagcagcag ccgctgccg ccccgcgga acggccata cccagaccg caacagatg gagaccaca caggagcct gaggcgtta gttgcacga cctacggtc aggctgccc acagccacg cacttggcc catcaccaa ctccagagt catcaccat tcagccaat ggcaggccc ggattgccg cagcaaggc ttgaagcgg ggcttgtcc gccgctgat gatgatgat catcacggc ttgcatcac gccaacggc gatatgaat ggagctaag aggatgatg tcggtggag gagcacagc aatgcggtt cgacctccg ctgactaga ggcgaatca cttccggcg gtttcgctg gcgcaaccg ttcgtcgtt tcgacggag aggaccttc aagcaggag aaacatcct ctcaggacg gcttcgttc gacaccgcc accgccttc aaacccaat gtccccgtt tcagtggcg agtgctaat ggttccagt tcggggtct cccttgaac aaccgcacg ggttcgccc cccgacccc aacgctggt accacccag caaactcag caatccggg ggtacgggc caaagcccc atggcggcc cttatgtca gtggctgat aatttaccc ccggggagc ccccgatcg gccggcggg agccccccg agcagtgca gcccctcga tcggcctcg agagggtcg caacactcg ccgaattcg acaggttcg tcgagtggc aggagatcg tcgggctct cgccacgtg tcttcgacc acggtcacc tcgacggaa gctgggggc gttatgggg cctaatgcc gagtctatg accggaagt ggcgatagc gtcgccccc acagcaccg gccaccacg gccactttg aagtgcact ctgtgccag gagagactg gaagacacg cactttgtc cagtgcccc tctgtacct caccacaag ttttgtttc ccttgcagt cgcgagagt atcaagaga caaggggct ggctctgag gtgtactgc cctagtggt gaaaagtgc ccattagcg aactccaac gtgccgtgg gcatttatg caaggcgaa atagcgacg atactcggc gaagagtac aaagtgaag aaagagcgg gagacttaa
- >TC007295 RA
ttccacgcc ggcaccgat gtttttaac atccttgta gaaaaaata gcggcgaag aggggttgc taaatcggg gttgcatgc ctgcgttta taaataccg aaatatcag cagcaagca gcgccaagt gcaggcaca ggatgtgaa tggggctct tagggttta ttacactga gcaggtctt ggggcaggc taaaattaa acctggatg tgggaaaat cgtggcgat tttccgaaa gtgaaggaa attaaagga aataatttc cggttcact tttccccat tgcgtcttc tcgttatcg aagatgggg tcataacgc ctgcgtcgt taaatagcc gggataatg ttggcggcg acggtgtta tttttacat gaacgaagt atcgaatag taaattcgt gacctaggc taatatcta gaacggggt aaaaatgag gaacgttcg aagggaagc acgaattgt gcgtttgcg tgcttgtgt cctgtgaaa atggcacga aaggttaag gagtgcgtc atttgctta aaggatgtg acttggcga tggttaagg gttgataag aaggtgtca gtttttgaa tagacgcag cgcggaatt caggtcaag tcgactgtc cgacctccc gtcggagac aggttggtc ggccaagcg tgattctgt tatttttgt taatttacg ttgtagtca agtgaagtg actcttgtt ggttctgct gttctttca gttcggatg ggatttatt ccagccagc agtcgtcga atgcagcgc gcgcaaact gtgacgtag accttattg ctattggtg ttcagtgcc tcgtgacgt catcaagct aacgattac cgagccagt cgagtgtct ctcccctgg ccccgttac cgttgtcgt tgcgttgat cgtcgggtt cagttagtt ctagcgcag cagcagtgt ttggatgtg ctcagagtg tgtccgcga ctatgtcgt agtgtccgt agtgacagt agtttgttc cgcaaaaag cagtgacgg aaataatca ttagatggt gaagatgtc ggtcatgca gatgcagca ggcgaaacg tcagcattg ctacttgtg cgacctgcc caggatgcc gtgggccat ggtccatga cttctccga gcccgtctg tcgagggtg cgtcaacta cgaaggcgc cgacaggat cgagatcgt cctggagac ggcacggca gatgaaacg agcccatgg cttccaaga gcagcgctc agcttcgca taagagcca ccggacggc cgcctccag ccatgagca ccagaacgg cgaagtggt ggcgacgtc cagccgaca acaacccgc cacgcacca ttcgggaac gtacagctt gcaccatcc gcgttccgg gatgttggc ggactacca agcccagca gcagccgct gccgccccg cggaacggc catacccag accgcaaca gatggagac cacacagga gcctgaggc gttagttgc acgacctac ggtcaggct gcccacagc cacgcactt ggcccatca ccaactcca gagtcatca ccattcagc caatggcag gcccggatt gccgcagca aggcttgaa gcggggctt gtccgccgc tgatgatga tgatcatca cggcttgca tcacgccaa cggcgatat gaatggagc taagaggat gatgtcggt ggaggagca cagcaatgc ggttcgacc tccgctgac tagaggcga atcacttcc ggcggtttc gctggcgca accgttcgt cgtttcgac ggagaggac cttcaagca ggagaaaca tcctctcag gacggcttc gttcgacac cgccaccgc cttcaaacc caatgtccc cgtttcagt ggcgagtgc taatggttc cagttcggg gtctccctt gaacaaccg cacgggttc gccccccga ccccaacgc tggtaccac ccagcaaac tcagcaatc cgggggtac gggccaaag ccccatggc ggcccttat gtcagtggc tgataattt acccccggg gagcccccg atcggccgg cgggagccc cccgagcag tgcagcccc tcgatcggc ctcgagagg gtcgcaaca ctcgccgaa ttcgacagg ttcgtcgag tggcaggag atcgtcggg ctctcgcca cgtgtcttc gaccacggt cacctcgac ggaagctgg gggcgttat ggggcctaa tgccgagtc tatgaccgg aagtggcga tagcgtcgc ccccacagc accggccac cacggccac tttgaagtg cactctgtg ccaggagag actggaaga cacgcactt tgtccagtg cccctctgt acctcacca caagttttg tttcccttg cagtcgcga gagtatcaa gagacaagg ggctggctc tgaggtgta ctgccctag tggtgaaaa gtgcccatt agcgaactc caacgtgcc gtgggcatt tatgcaagg cgaaatagc gacgatact cggcgaaga gtacaaagt gaagaaaga gcgggagac ttaatattg gatgtgcta agtacgaag tacgaggct ctctttaac tcgacaaca gtcgagaca tgtctcttt tacaaaatg aaagagatg atttgttga aaagttgct ggcgaaagt ctgaagatg gtggattag taaaaaata cggtttgtc ggttaacgg ttgattgtt aaattgaag cgaagaagg ctgtgtcga atatattag ttttatctt ggacgaatc gtccatctg tccgtctta tgattgtgt acccaccga attaaaagt aaaaaacaa aaaaaaaca tggctaacg tcgccgaaa atgtacata taaaagagt cgggtagtg aaaagattt cgttttgtt tatttgttt caagtcaat ttctgccta aacttcttc tgatttgtg tattggacg ttttacttt gtaaatatt gtacataaa aatcgtgct aaaatttgc aaaaaatgt acaaaatat attatttaa gttagttta gtcttgaag gaataaatt taatacgca caactagcc agtacggat ttatacctt ttgtaacaa actacttgt ctagttgtt agtgtgaaa tttatttat ttggagtat gactacgtt ttatttttt cgtttaaaa ctattcata ctataaaag gcctcttac tgaaaagct gtaaatatt gtatattca tcagctatt taacgacat gaaaatcga ccagatttt aaaaatgcc gttcttatt agtttagaa acttcgtta ttaatggta cacttgggc catggcttt ttttaattt atggaaagt aacctagtc aaaacgtga agtgtctca cacttcatt tataggaac tcaaaactc gctggtata ttgtaagaa cgataatta aagaaaatc cttgattaa aataacatt taacttgta tatttatac catgtttaa tgttcaagg tctcctcac atttgtttg cttgtttag tgagaatag aacagttca agaaatttt ttcatttcc catcagtcg ctttaaatc agtttattc ttcctaaac aaagcttca aacgaaacg accacattg aattattgt atagacctt gtaaataaa tacttgtat taccctaaa acgacattg ttaacccca ttcattgac agaagtaac cgctccact tggattttt cgcaaacgt tacgcaaga tctcgcagc aggtcgcga acttttcaa cgaattttt aacactgtt gtgattttt tgcgtagcg ttgccaccc ctgaaatat tgtgagttt cacgtccat cagaagtga gggattaca tttttactt ctttggccc taaaataga ggacttgta acattgaga gttttgaaa tttgtgata aatgcacag atatttgta ttgacaatg tcaatcttg tacattttg tagttgtta atttgtata acaactgac atctaattt gtttggaaa gcttgtata atctttgct gttagtgga tgtcaaata aaatgagat aatcaat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
