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Gene information
General information
TC007295 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6439389..6449707
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007295
    iBeetle screen iB_01161

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 7


    elytra partially blistered - Number of animals showing the phenotype: 7


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007295 RA
      MSVMQMQQA KRQHCYLCD LPRMPWAMV HDFSEPVCR 
GCVNYEGAD RIEIVLETA RQMKRAHGF QEQRSASHK 
SHRTAASSH EHQNGEVVA TSSRQQPAT HHSGTYSLH 
HPRSGMLAD YQAQQQPLP PRGTAIPRP QQMETTQEP 
EALVARPTV RLPTATHLA HHQLQSHHH SANGRPGLP 
QQGLKRGLS AADDDDHHG LHHANGDMN GAKRMMSVE 
EHSNAVRPP LTRGESLPA VSLAQPFVV STERTFKQE 
KHPLRTASF DTATAFKPN VPVSVASAN GSSSGSPLN 
NRTGSPPDP NAGTTQQTQ QSGGTGQSP MAALMSVAD 
NLPPGSPRS AGGSPPSSA APRSASRGS QHSPNSTGS 
SSGRRSSGS RHVSSTTVT STEAGGVMG PNAESMTGS 
GDSVAPTAP ATTATLKCT LCQERLEDT HFVQCPSVP 
HHKFCFPCS RESIKRQGA GSEVYCPSG EKCPLANSN 
VPWAFMQGE IATILGEEY KVKKERET
    CDS sequence
    • >TC007295 RA
      atgtcggtc atgcagatg cagcaggcg aaacgtcag 
cattgctac ttgtgcgac ctgcccagg atgccgtgg 
gccatggtc catgacttc tccgagccc gtctgtcga 
gggtgcgtc aactacgaa ggcgccgac aggatcgag 
atcgtcctg gagacggca cggcagatg aaacgagcc 
catggcttc caagagcag cgctcagct tcgcataag 
agccaccgg acggccgcc tccagccat gagcaccag 
aacggcgaa gtggtggcg acgtccagc cgacaacaa 
cccgccacg caccattcg ggaacgtac agcttgcac 
catccgcgt tccgggatg ttggcggac taccaagcc 
cagcagcag ccgctgccg ccccgcgga acggccata 
cccagaccg caacagatg gagaccaca caggagcct 
gaggcgtta gttgcacga cctacggtc aggctgccc 
acagccacg cacttggcc catcaccaa ctccagagt 
catcaccat tcagccaat ggcaggccc ggattgccg 
cagcaaggc ttgaagcgg ggcttgtcc gccgctgat 
gatgatgat catcacggc ttgcatcac gccaacggc 
gatatgaat ggagctaag aggatgatg tcggtggag 
gagcacagc aatgcggtt cgacctccg ctgactaga 
ggcgaatca cttccggcg gtttcgctg gcgcaaccg 
ttcgtcgtt tcgacggag aggaccttc aagcaggag 
aaacatcct ctcaggacg gcttcgttc gacaccgcc 
accgccttc aaacccaat gtccccgtt tcagtggcg 
agtgctaat ggttccagt tcggggtct cccttgaac 
aaccgcacg ggttcgccc cccgacccc aacgctggt 
accacccag caaactcag caatccggg ggtacgggc 
caaagcccc atggcggcc cttatgtca gtggctgat 
aatttaccc ccggggagc ccccgatcg gccggcggg 
agccccccg agcagtgca gcccctcga tcggcctcg 
agagggtcg caacactcg ccgaattcg acaggttcg 
tcgagtggc aggagatcg tcgggctct cgccacgtg 
tcttcgacc acggtcacc tcgacggaa gctgggggc 
gttatgggg cctaatgcc gagtctatg accggaagt 
ggcgatagc gtcgccccc acagcaccg gccaccacg 
gccactttg aagtgcact ctgtgccag gagagactg 
gaagacacg cactttgtc cagtgcccc tctgtacct 
caccacaag ttttgtttc ccttgcagt cgcgagagt 
atcaagaga caaggggct ggctctgag gtgtactgc 
cctagtggt gaaaagtgc ccattagcg aactccaac 
gtgccgtgg gcatttatg caaggcgaa atagcgacg 
atactcggc gaagagtac aaagtgaag aaagagcgg 
gagacttaa
    mRNA sequence
    • >TC007295 RA
      ttccacgcc ggcaccgat gtttttaac atccttgta 
gaaaaaata gcggcgaag aggggttgc taaatcggg 
gttgcatgc ctgcgttta taaataccg aaatatcag 
cagcaagca gcgccaagt gcaggcaca ggatgtgaa 
tggggctct tagggttta ttacactga gcaggtctt 
ggggcaggc taaaattaa acctggatg tgggaaaat 
cgtggcgat tttccgaaa gtgaaggaa attaaagga 
aataatttc cggttcact tttccccat tgcgtcttc 
tcgttatcg aagatgggg tcataacgc ctgcgtcgt 
taaatagcc gggataatg ttggcggcg acggtgtta 
tttttacat gaacgaagt atcgaatag taaattcgt 
gacctaggc taatatcta gaacggggt aaaaatgag 
gaacgttcg aagggaagc acgaattgt gcgtttgcg 
tgcttgtgt cctgtgaaa atggcacga aaggttaag 
gagtgcgtc atttgctta aaggatgtg acttggcga 
tggttaagg gttgataag aaggtgtca gtttttgaa 
tagacgcag cgcggaatt caggtcaag tcgactgtc 
cgacctccc gtcggagac aggttggtc ggccaagcg 
tgattctgt tatttttgt taatttacg ttgtagtca 
agtgaagtg actcttgtt ggttctgct gttctttca 
gttcggatg ggatttatt ccagccagc agtcgtcga 
atgcagcgc gcgcaaact gtgacgtag accttattg 
ctattggtg ttcagtgcc tcgtgacgt catcaagct 
aacgattac cgagccagt cgagtgtct ctcccctgg 
ccccgttac cgttgtcgt tgcgttgat cgtcgggtt 
cagttagtt ctagcgcag cagcagtgt ttggatgtg 
ctcagagtg tgtccgcga ctatgtcgt agtgtccgt 
agtgacagt agtttgttc cgcaaaaag cagtgacgg 
aaataatca ttagatggt gaagatgtc ggtcatgca 
gatgcagca ggcgaaacg tcagcattg ctacttgtg 
cgacctgcc caggatgcc gtgggccat ggtccatga 
cttctccga gcccgtctg tcgagggtg cgtcaacta 
cgaaggcgc cgacaggat cgagatcgt cctggagac 
ggcacggca gatgaaacg agcccatgg cttccaaga 
gcagcgctc agcttcgca taagagcca ccggacggc 
cgcctccag ccatgagca ccagaacgg cgaagtggt 
ggcgacgtc cagccgaca acaacccgc cacgcacca 
ttcgggaac gtacagctt gcaccatcc gcgttccgg 
gatgttggc ggactacca agcccagca gcagccgct 
gccgccccg cggaacggc catacccag accgcaaca 
gatggagac cacacagga gcctgaggc gttagttgc 
acgacctac ggtcaggct gcccacagc cacgcactt 
ggcccatca ccaactcca gagtcatca ccattcagc 
caatggcag gcccggatt gccgcagca aggcttgaa 
gcggggctt gtccgccgc tgatgatga tgatcatca 
cggcttgca tcacgccaa cggcgatat gaatggagc 
taagaggat gatgtcggt ggaggagca cagcaatgc 
ggttcgacc tccgctgac tagaggcga atcacttcc 
ggcggtttc gctggcgca accgttcgt cgtttcgac 
ggagaggac cttcaagca ggagaaaca tcctctcag 
gacggcttc gttcgacac cgccaccgc cttcaaacc 
caatgtccc cgtttcagt ggcgagtgc taatggttc 
cagttcggg gtctccctt gaacaaccg cacgggttc 
gccccccga ccccaacgc tggtaccac ccagcaaac 
tcagcaatc cgggggtac gggccaaag ccccatggc 
ggcccttat gtcagtggc tgataattt acccccggg 
gagcccccg atcggccgg cgggagccc cccgagcag 
tgcagcccc tcgatcggc ctcgagagg gtcgcaaca 
ctcgccgaa ttcgacagg ttcgtcgag tggcaggag 
atcgtcggg ctctcgcca cgtgtcttc gaccacggt 
cacctcgac ggaagctgg gggcgttat ggggcctaa 
tgccgagtc tatgaccgg aagtggcga tagcgtcgc 
ccccacagc accggccac cacggccac tttgaagtg 
cactctgtg ccaggagag actggaaga cacgcactt 
tgtccagtg cccctctgt acctcacca caagttttg 
tttcccttg cagtcgcga gagtatcaa gagacaagg 
ggctggctc tgaggtgta ctgccctag tggtgaaaa 
gtgcccatt agcgaactc caacgtgcc gtgggcatt 
tatgcaagg cgaaatagc gacgatact cggcgaaga 
gtacaaagt gaagaaaga gcgggagac ttaatattg 
gatgtgcta agtacgaag tacgaggct ctctttaac 
tcgacaaca gtcgagaca tgtctcttt tacaaaatg 
aaagagatg atttgttga aaagttgct ggcgaaagt 
ctgaagatg gtggattag taaaaaata cggtttgtc 
ggttaacgg ttgattgtt aaattgaag cgaagaagg 
ctgtgtcga atatattag ttttatctt ggacgaatc 
gtccatctg tccgtctta tgattgtgt acccaccga 
attaaaagt aaaaaacaa aaaaaaaca tggctaacg 
tcgccgaaa atgtacata taaaagagt cgggtagtg 
aaaagattt cgttttgtt tatttgttt caagtcaat 
ttctgccta aacttcttc tgatttgtg tattggacg 
ttttacttt gtaaatatt gtacataaa aatcgtgct 
aaaatttgc aaaaaatgt acaaaatat attatttaa 
gttagttta gtcttgaag gaataaatt taatacgca 
caactagcc agtacggat ttatacctt ttgtaacaa 
actacttgt ctagttgtt agtgtgaaa tttatttat 
ttggagtat gactacgtt ttatttttt cgtttaaaa 
ctattcata ctataaaag gcctcttac tgaaaagct 
gtaaatatt gtatattca tcagctatt taacgacat 
gaaaatcga ccagatttt aaaaatgcc gttcttatt 
agtttagaa acttcgtta ttaatggta cacttgggc 
catggcttt ttttaattt atggaaagt aacctagtc 
aaaacgtga agtgtctca cacttcatt tataggaac 
tcaaaactc gctggtata ttgtaagaa cgataatta 
aagaaaatc cttgattaa aataacatt taacttgta 
tatttatac catgtttaa tgttcaagg tctcctcac 
atttgtttg cttgtttag tgagaatag aacagttca 
agaaatttt ttcatttcc catcagtcg ctttaaatc 
agtttattc ttcctaaac aaagcttca aacgaaacg 
accacattg aattattgt atagacctt gtaaataaa 
tacttgtat taccctaaa acgacattg ttaacccca 
ttcattgac agaagtaac cgctccact tggattttt 
cgcaaacgt tacgcaaga tctcgcagc aggtcgcga 
acttttcaa cgaattttt aacactgtt gtgattttt 
tgcgtagcg ttgccaccc ctgaaatat tgtgagttt 
cacgtccat cagaagtga gggattaca tttttactt 
ctttggccc taaaataga ggacttgta acattgaga 
gttttgaaa tttgtgata aatgcacag atatttgta 
ttgacaatg tcaatcttg tacattttg tagttgtta 
atttgtata acaactgac atctaattt gtttggaaa 
gcttgtata atctttgct gttagtgga tgtcaaata 
aaatgagat aatcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY