Status
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Gene information
General information
TC007295 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6439389..6449707
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007295
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01161

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Adult morphology
    wings not closed - Number of animals showing the phenotype: 7


    elytra partially blistered - Number of animals showing the phenotype: 7


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007295 RA
      MSVMQMQQA KRQHCYLCD LPRMPWAMV HDFSEPVCR 
      GCVNYEGAD RIEIVLETA RQMKRAHGF QEQRSASHK 
      SHRTAASSH EHQNGEVVA TSSRQQPAT HHSGTYSLH 
      HPRSGMLAD YQAQQQPLP PRGTAIPRP QQMETTQEP 
      EALVARPTV RLPTATHLA HHQLQSHHH SANGRPGLP 
      QQGLKRGLS AADDDDHHG LHHANGDMN GAKRMMSVE 
      EHSNAVRPP LTRGESLPA VSLAQPFVV STERTFKQE 
      KHPLRTASF DTATAFKPN VPVSVASAN GSSSGSPLN 
      NRTGSPPDP NAGTTQQTQ QSGGTGQSP MAALMSVAD 
      NLPPGSPRS AGGSPPSSA APRSASRGS QHSPNSTGS 
      SSGRRSSGS RHVSSTTVT STEAGGVMG PNAESMTGS 
      GDSVAPTAP ATTATLKCT LCQERLEDT HFVQCPSVP 
      HHKFCFPCS RESIKRQGA GSEVYCPSG EKCPLANSN 
      VPWAFMQGE IATILGEEY KVKKERET
    CDS sequence
    • >TC007295 RA
      atgtcggtc atgcagatg cagcaggcg aaacgtcag 
      cattgctac ttgtgcgac ctgcccagg atgccgtgg 
      gccatggtc catgacttc tccgagccc gtctgtcga 
      gggtgcgtc aactacgaa ggcgccgac aggatcgag 
      atcgtcctg gagacggca cggcagatg aaacgagcc 
      catggcttc caagagcag cgctcagct tcgcataag 
      agccaccgg acggccgcc tccagccat gagcaccag 
      aacggcgaa gtggtggcg acgtccagc cgacaacaa 
      cccgccacg caccattcg ggaacgtac agcttgcac 
      catccgcgt tccgggatg ttggcggac taccaagcc 
      cagcagcag ccgctgccg ccccgcgga acggccata 
      cccagaccg caacagatg gagaccaca caggagcct 
      gaggcgtta gttgcacga cctacggtc aggctgccc 
      acagccacg cacttggcc catcaccaa ctccagagt 
      catcaccat tcagccaat ggcaggccc ggattgccg 
      cagcaaggc ttgaagcgg ggcttgtcc gccgctgat 
      gatgatgat catcacggc ttgcatcac gccaacggc 
      gatatgaat ggagctaag aggatgatg tcggtggag 
      gagcacagc aatgcggtt cgacctccg ctgactaga 
      ggcgaatca cttccggcg gtttcgctg gcgcaaccg 
      ttcgtcgtt tcgacggag aggaccttc aagcaggag 
      aaacatcct ctcaggacg gcttcgttc gacaccgcc 
      accgccttc aaacccaat gtccccgtt tcagtggcg 
      agtgctaat ggttccagt tcggggtct cccttgaac 
      aaccgcacg ggttcgccc cccgacccc aacgctggt 
      accacccag caaactcag caatccggg ggtacgggc 
      caaagcccc atggcggcc cttatgtca gtggctgat 
      aatttaccc ccggggagc ccccgatcg gccggcggg 
      agccccccg agcagtgca gcccctcga tcggcctcg 
      agagggtcg caacactcg ccgaattcg acaggttcg 
      tcgagtggc aggagatcg tcgggctct cgccacgtg 
      tcttcgacc acggtcacc tcgacggaa gctgggggc 
      gttatgggg cctaatgcc gagtctatg accggaagt 
      ggcgatagc gtcgccccc acagcaccg gccaccacg 
      gccactttg aagtgcact ctgtgccag gagagactg 
      gaagacacg cactttgtc cagtgcccc tctgtacct 
      caccacaag ttttgtttc ccttgcagt cgcgagagt 
      atcaagaga caaggggct ggctctgag gtgtactgc 
      cctagtggt gaaaagtgc ccattagcg aactccaac 
      gtgccgtgg gcatttatg caaggcgaa atagcgacg 
      atactcggc gaagagtac aaagtgaag aaagagcgg 
      gagacttaa
    mRNA sequence
    • >TC007295 RA
      ttccacgcc ggcaccgat gtttttaac atccttgta 
      gaaaaaata gcggcgaag aggggttgc taaatcggg 
      gttgcatgc ctgcgttta taaataccg aaatatcag 
      cagcaagca gcgccaagt gcaggcaca ggatgtgaa 
      tggggctct tagggttta ttacactga gcaggtctt 
      ggggcaggc taaaattaa acctggatg tgggaaaat 
      cgtggcgat tttccgaaa gtgaaggaa attaaagga 
      aataatttc cggttcact tttccccat tgcgtcttc 
      tcgttatcg aagatgggg tcataacgc ctgcgtcgt 
      taaatagcc gggataatg ttggcggcg acggtgtta 
      tttttacat gaacgaagt atcgaatag taaattcgt 
      gacctaggc taatatcta gaacggggt aaaaatgag 
      gaacgttcg aagggaagc acgaattgt gcgtttgcg 
      tgcttgtgt cctgtgaaa atggcacga aaggttaag 
      gagtgcgtc atttgctta aaggatgtg acttggcga 
      tggttaagg gttgataag aaggtgtca gtttttgaa 
      tagacgcag cgcggaatt caggtcaag tcgactgtc 
      cgacctccc gtcggagac aggttggtc ggccaagcg 
      tgattctgt tatttttgt taatttacg ttgtagtca 
      agtgaagtg actcttgtt ggttctgct gttctttca 
      gttcggatg ggatttatt ccagccagc agtcgtcga 
      atgcagcgc gcgcaaact gtgacgtag accttattg 
      ctattggtg ttcagtgcc tcgtgacgt catcaagct 
      aacgattac cgagccagt cgagtgtct ctcccctgg 
      ccccgttac cgttgtcgt tgcgttgat cgtcgggtt 
      cagttagtt ctagcgcag cagcagtgt ttggatgtg 
      ctcagagtg tgtccgcga ctatgtcgt agtgtccgt 
      agtgacagt agtttgttc cgcaaaaag cagtgacgg 
      aaataatca ttagatggt gaagatgtc ggtcatgca 
      gatgcagca ggcgaaacg tcagcattg ctacttgtg 
      cgacctgcc caggatgcc gtgggccat ggtccatga 
      cttctccga gcccgtctg tcgagggtg cgtcaacta 
      cgaaggcgc cgacaggat cgagatcgt cctggagac 
      ggcacggca gatgaaacg agcccatgg cttccaaga 
      gcagcgctc agcttcgca taagagcca ccggacggc 
      cgcctccag ccatgagca ccagaacgg cgaagtggt 
      ggcgacgtc cagccgaca acaacccgc cacgcacca 
      ttcgggaac gtacagctt gcaccatcc gcgttccgg 
      gatgttggc ggactacca agcccagca gcagccgct 
      gccgccccg cggaacggc catacccag accgcaaca 
      gatggagac cacacagga gcctgaggc gttagttgc 
      acgacctac ggtcaggct gcccacagc cacgcactt 
      ggcccatca ccaactcca gagtcatca ccattcagc 
      caatggcag gcccggatt gccgcagca aggcttgaa 
      gcggggctt gtccgccgc tgatgatga tgatcatca 
      cggcttgca tcacgccaa cggcgatat gaatggagc 
      taagaggat gatgtcggt ggaggagca cagcaatgc 
      ggttcgacc tccgctgac tagaggcga atcacttcc 
      ggcggtttc gctggcgca accgttcgt cgtttcgac 
      ggagaggac cttcaagca ggagaaaca tcctctcag 
      gacggcttc gttcgacac cgccaccgc cttcaaacc 
      caatgtccc cgtttcagt ggcgagtgc taatggttc 
      cagttcggg gtctccctt gaacaaccg cacgggttc 
      gccccccga ccccaacgc tggtaccac ccagcaaac 
      tcagcaatc cgggggtac gggccaaag ccccatggc 
      ggcccttat gtcagtggc tgataattt acccccggg 
      gagcccccg atcggccgg cgggagccc cccgagcag 
      tgcagcccc tcgatcggc ctcgagagg gtcgcaaca 
      ctcgccgaa ttcgacagg ttcgtcgag tggcaggag 
      atcgtcggg ctctcgcca cgtgtcttc gaccacggt 
      cacctcgac ggaagctgg gggcgttat ggggcctaa 
      tgccgagtc tatgaccgg aagtggcga tagcgtcgc 
      ccccacagc accggccac cacggccac tttgaagtg 
      cactctgtg ccaggagag actggaaga cacgcactt 
      tgtccagtg cccctctgt acctcacca caagttttg 
      tttcccttg cagtcgcga gagtatcaa gagacaagg 
      ggctggctc tgaggtgta ctgccctag tggtgaaaa 
      gtgcccatt agcgaactc caacgtgcc gtgggcatt 
      tatgcaagg cgaaatagc gacgatact cggcgaaga 
      gtacaaagt gaagaaaga gcgggagac ttaatattg 
      gatgtgcta agtacgaag tacgaggct ctctttaac 
      tcgacaaca gtcgagaca tgtctcttt tacaaaatg 
      aaagagatg atttgttga aaagttgct ggcgaaagt 
      ctgaagatg gtggattag taaaaaata cggtttgtc 
      ggttaacgg ttgattgtt aaattgaag cgaagaagg 
      ctgtgtcga atatattag ttttatctt ggacgaatc 
      gtccatctg tccgtctta tgattgtgt acccaccga 
      attaaaagt aaaaaacaa aaaaaaaca tggctaacg 
      tcgccgaaa atgtacata taaaagagt cgggtagtg 
      aaaagattt cgttttgtt tatttgttt caagtcaat 
      ttctgccta aacttcttc tgatttgtg tattggacg 
      ttttacttt gtaaatatt gtacataaa aatcgtgct 
      aaaatttgc aaaaaatgt acaaaatat attatttaa 
      gttagttta gtcttgaag gaataaatt taatacgca 
      caactagcc agtacggat ttatacctt ttgtaacaa 
      actacttgt ctagttgtt agtgtgaaa tttatttat 
      ttggagtat gactacgtt ttatttttt cgtttaaaa 
      ctattcata ctataaaag gcctcttac tgaaaagct 
      gtaaatatt gtatattca tcagctatt taacgacat 
      gaaaatcga ccagatttt aaaaatgcc gttcttatt 
      agtttagaa acttcgtta ttaatggta cacttgggc 
      catggcttt ttttaattt atggaaagt aacctagtc 
      aaaacgtga agtgtctca cacttcatt tataggaac 
      tcaaaactc gctggtata ttgtaagaa cgataatta 
      aagaaaatc cttgattaa aataacatt taacttgta 
      tatttatac catgtttaa tgttcaagg tctcctcac 
      atttgtttg cttgtttag tgagaatag aacagttca 
      agaaatttt ttcatttcc catcagtcg ctttaaatc 
      agtttattc ttcctaaac aaagcttca aacgaaacg 
      accacattg aattattgt atagacctt gtaaataaa 
      tacttgtat taccctaaa acgacattg ttaacccca 
      ttcattgac agaagtaac cgctccact tggattttt 
      cgcaaacgt tacgcaaga tctcgcagc aggtcgcga 
      acttttcaa cgaattttt aacactgtt gtgattttt 
      tgcgtagcg ttgccaccc ctgaaatat tgtgagttt 
      cacgtccat cagaagtga gggattaca tttttactt 
      ctttggccc taaaataga ggacttgta acattgaga 
      gttttgaaa tttgtgata aatgcacag atatttgta 
      ttgacaatg tcaatcttg tacattttg tagttgtta 
      atttgtata acaactgac atctaattt gtttggaaa 
      gcttgtata atctttgct gttagtgga tgtcaaata 
      aaatgagat aatcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY