| General information | |||
| TC032839
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 6593331..6604104 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032839 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01159 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature coxal musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
elytra blistered - Number of animals showing the phenotype: 2
wings not closed - Number of animals showing the phenotype: 3
- >TC032839 RA
MRDLATKMA ELKFEAPLA QFEESDEWG STEFQNATI KNENELNNI NLNKKIKDI NDVLNDFNE DILNNSNTE TLPPKNVIL KNGETASVA DNFTETFSG SLEDLVNTF DEKITKCFG NYEESVEKL APVQVRTQE EIMNECHCA CSWAHRVPC LNQTGSVLT SNEANYTTK ATCLMANSV ILTGERLEP LGASDDRKS EIVRNSGCD KCVCLENEK DFLQTSLFT LTTYFAQVQ FRLRQVVDA PFEDQEELL KSLEEFAFT DVNLRDSND SLGDALLET VKKRREQQK ILLGRLKSQ LRRLKEFDW NELVEHVRL KENLEQQLK TQIVDLERF IQYLRNVDT KCVCKRSRK SFGSFLVEA IDRIFAFLQ MFALLQFTW GSNCFASDR INQWADLRA RLELAIIDL KKTLHSNED AHKITTIVR SNLATSIKD LMEHGTKSH PFLGCLSHQ ATHGWDIIL FYYHFKRGV SPKTSKKNL LCAVEEIIS THSRYKRSH DSHFRAFVC AALNAKRLV PWLNLIFHC RRVIETFYY PWSYVATTG YQDSLRSLE ALTPLKFDL PVDLDVRKF QIEDNPKPL ACGSERLPA RPYANGYIT LFITQIVSR MRSRLTSRS FRMWWTITG NFGNILPID WSKSYARKL HMPALNLNE KEATTPDDD LELSSEDEA VASDLDMHA LILGGLHQD TEPLKTADE VIQEIDDMM QEETCSLDG SPGSRDSII ETNETLEKA KEVLSSPLY EDKLRDLSL SQLNELFLE LEVLIREFS ETLISELAL RDELEYEKE LKNTFISLL LAVQNKRRQ YHVEKKRSL KNASKTPSG LDPKYLTTV IPYHLDSGP PDNQTLQVL IKILKAINE DSPTVPTLL TDYILKVIC PT
- >TC032839 RB
MRDLATKMA ELKFEAPLA QFEESDEWG STEFQNATI KNENELNNI NLNKKIKDI NDVLNDFNE DILNNSNTE TLPPKNVIL KNGETASVA DNFTETFSG SLEDLVNTF DEKITKCFG NYEESVEKL APVQVRTQE EIMNECHCA CSWAHRVPC LNQTGSVLT SNEANYTTK ATCLMANSV ILTGERLEP LGASDDRKS EIVRNSGCD KCVCLENEK DFLQTSLFT LTTYFAQVQ FRLRQVVDA PFEDQEELL KSLEEFAFT DVNLRDSND SLGDALLET VKKRREQQK ILLGRLKSQ LRRLKEFDW NELVEHVRL KENLEQQLK TQIVDLERF IQYLRNVDT KCVCKRSRK SFGSFLVEA IDRIFAFLQ MFALLQFTW GSNCFASDR INQWADLRA RLELAIIDL KKTLHSNED AHKITTIVR SNLATSIKD LMEHGTKSH PFLGCLSHQ ATHGWDIIL FYYHFKRGD EYNSSTVQK LSRSFNLAI AGVSPKTSK KNLLCAVEE IISTHSRYK RSHDSHFRA FVCAALNAK RLVPWLNLI FHCRRVIET FYYPWSYVA TTGYQDSLR SLEALTPLK FDLPVDLDV RKFQIEDNP KPLACGSER LPARPYANG YITLFITQI VSRMRSRLT SRSFRMWWT ITGNFGNIL PIDWSKSYA RKLHMPALN LNEKEATTP DDDLELSSE DEAVASDLD MHALILGGL HQDTEPLKT ADEVIQEID DMMQEETCS LDGSPGSRD SIIETNETL EKAKEVLSS PLYEDKLRD LSLSQLNEL FLELEVLIR EFSETLISE LALRDELEY EKELKNTFI SLLLAVQNK RRQYHVEKK RSLKNASKT PSGLDPKYL TTVIPYHLD SGPPDNQTL QVLIKILKA INEDSPTVP TLLTDYILK VICPT
- >TC032839 RA
atgcgggat ttggccaca aaaatggcc gaactaaaa tttgaagca ccactggcc caattcgag gaatcggat gaatggggt tcgaccgag tttcaaaac gccaccata aaaaacgag aacgaactg aacaacatc aacctgaac aaaaaaatc aaagacatc aacgacgtc ctgaacgat ttcaatgag gacatcctc aacaattca aacaccgaa accttgccg ccaaaaaac gtgattttg aaaaacggt gaaacggcc tcggtggcc gacaacttc accgaaact ttctcgggc tcgcttgag gacctcgtt aacacgttc gatgaaaaa atcaccaag tgcttcggc aactacgag gagtcggtg gagaagttg gcccccgtc caggtccgg acgcaggag gagatcatg aacgaatgc cattgtgca tgttcgtgg gcacatcgc gtgccttgt ttgaaccaa actggctct gtgcttacg tcaaatgag gctaattac accactaaa gccacctgt ttaatggcc aatagtgtc atcttgaca ggtgaacgg ttggagcct ctcggcgcg tccgatgac cgcaaaagc gaaattgtt cgaaatagt ggatgcgac aaatgtgtg tgtttggaa aacgagaaa gacttcctc cagacttcg ctatttact ttaacgacg tacttcgcc caagtccag ttccggctg cggcaagtc gtcgacgcg ccgttcgag gaccaagaa gagttgtta aaatcgctg gaggagttt gcgttcact gatgtaaat ctccgggat agtaatgat agcctcggt gacgcgttg ctggaaacg gtcaaaaag cggcgcgag caacagaaa attttgttg ggacggttg aaatcacag ttgagacga ttaaaagag tttgactgg aacgaatta gtggaacat gtacggcta aaggaaaat ttggagcaa caacttaag acgcagata gttgatttg gaacggttt attcaatat ttaaggaac gtggatact aaatgtgtg tgtaaacgg agtaggaaa agcttcggg tcgtttctc gtcgaagcc atcgatcgg atttttgca tttttgcaa atgtttgcc ttgctacag tttacttgg ggttcgaat tgcttcgcc tcggaccgg atcaaccaa tgggctgat ctgagagcc agactcgag cttgccata atcgacttg aagaaaacg ttgcacagt aatgaagac gcgcacaaa atcacaaca atcgttcgg agtaattta gccacaagc atcaaagac ttgatggag cacgggaca aagtcccac ccgttcctg ggttgtctg tcgcatcag gcaacacat ggctgggac atcatcctg ttttactac cattttaaa agaggtgtt tcacccaaa actagcaag aaaaattta ctgtgcgcc gtcgaagag atcatttcg acgcattcg cggtacaaa aggagccac gattcgcac tttagagcc ttcgtttgt gcggctcta aatgccaaa agacttgtg ccctggtta aatttaatc ttccactgt cggcgggtt atcgaaaca ttttattat ccgtggagc tacgttgcc acgaccgga taccaagac agtctgagg agtttggag gctctgaca ccgctcaag tttgatctc ccggttgat ttggacgtg cgaaagttc caaattgag gataaccca aaaccccta gcatgtggg tcggaacgg ctcccagca aggccgtat gcaaatggt tacatcact ctttttatt acacaaatt gtctcccgt atgaggtca agactaacg agtcgttct ttcagaatg tggtggacc ataacggga aacttcggc aacattctg cccattgac tggtccaaa tcatacgcc aggaaactg cacatgcca gctctcaat ttaaacgag aaggaagcc acaacgccc gacgacgac ttggaatta agttcagag gacgaggct gtcgccagt gatttagac atgcacgcc ctcatcctg ggcggtttg caccaagac accgagccg cttaagacc gcagacgaa gtcatccag gagattgac gacatgatg caggaggag acgtgttcg ctagacggc tctccggga tccagggac tccataatt gagacgaat gagacgctg gaaaaggcg aaggaggtt ttaagctcg ccgttgtat gaagacaaa ttacgcgac ttgagtctg tcgcagtta aatgaatta tttctggaa ctagaggtt ttaattagg gaattttcc gaaacgctt atctcggag ttggccctc agagacgag ttggagtac gagaaggag ctgaagaac acttttatt tccctactt cttgccgtt caaaataaa cgtagacaa taccatgtt gagaagaag cggagttta aaaaatgcg tccaagact cccagcgga ctggacccg aaatattta accactgtc attccgtac catttagat agcggacca ccggataat caaacgtta caagtcctg attaaaatt ttgaaagcc ataaatgaa gacagcccc acagttccc actttatta acggactat atcctaaaa gttatatgt ccgacgtaa
- >TC032839 RB
atgcgggat ttggccaca aaaatggcc gaactaaaa tttgaagca ccactggcc caattcgag gaatcggat gaatggggt tcgaccgag tttcaaaac gccaccata aaaaacgag aacgaactg aacaacatc aacctgaac aaaaaaatc aaagacatc aacgacgtc ctgaacgat ttcaatgag gacatcctc aacaattca aacaccgaa accttgccg ccaaaaaac gtgattttg aaaaacggt gaaacggcc tcggtggcc gacaacttc accgaaact ttctcgggc tcgcttgag gacctcgtt aacacgttc gatgaaaaa atcaccaag tgcttcggc aactacgag gagtcggtg gagaagttg gcccccgtc caggtccgg acgcaggag gagatcatg aacgaatgc cattgtgca tgttcgtgg gcacatcgc gtgccttgt ttgaaccaa actggctct gtgcttacg tcaaatgag gctaattac accactaaa gccacctgt ttaatggcc aatagtgtc atcttgaca ggtgaacgg ttggagcct ctcggcgcg tccgatgac cgcaaaagc gaaattgtt cgaaatagt ggatgcgac aaatgtgtg tgtttggaa aacgagaaa gacttcctc cagacttcg ctatttact ttaacgacg tacttcgcc caagtccag ttccggctg cggcaagtc gtcgacgcg ccgttcgag gaccaagaa gagttgtta aaatcgctg gaggagttt gcgttcact gatgtaaat ctccgggat agtaatgat agcctcggt gacgcgttg ctggaaacg gtcaaaaag cggcgcgag caacagaaa attttgttg ggacggttg aaatcacag ttgagacga ttaaaagag tttgactgg aacgaatta gtggaacat gtacggcta aaggaaaat ttggagcaa caacttaag acgcagata gttgatttg gaacggttt attcaatat ttaaggaac gtggatact aaatgtgtg tgtaaacgg agtaggaaa agcttcggg tcgtttctc gtcgaagcc atcgatcgg atttttgca tttttgcaa atgtttgcc ttgctacag tttacttgg ggttcgaat tgcttcgcc tcggaccgg atcaaccaa tgggctgat ctgagagcc agactcgag cttgccata atcgacttg aagaaaacg ttgcacagt aatgaagac gcgcacaaa atcacaaca atcgttcgg agtaattta gccacaagc atcaaagac ttgatggag cacgggaca aagtcccac ccgttcctg ggttgtctg tcgcatcag gcaacacat ggctgggac atcatcctg ttttactac cattttaaa agaggtgat gaatacaac tcgtcaacg gttcagaag ttatcgcga agtttcaat cttgcaatt gcaggtgtt tcacccaaa actagcaag aaaaattta ctgtgcgcc gtcgaagag atcatttcg acgcattcg cggtacaaa aggagccac gattcgcac tttagagcc ttcgtttgt gcggctcta aatgccaaa agacttgtg ccctggtta aatttaatc ttccactgt cggcgggtt atcgaaaca ttttattat ccgtggagc tacgttgcc acgaccgga taccaagac agtctgagg agtttggag gctctgaca ccgctcaag tttgatctc ccggttgat ttggacgtg cgaaagttc caaattgag gataaccca aaaccccta gcatgtggg tcggaacgg ctcccagca aggccgtat gcaaatggt tacatcact ctttttatt acacaaatt gtctcccgt atgaggtca agactaacg agtcgttct ttcagaatg tggtggacc ataacggga aacttcggc aacattctg cccattgac tggtccaaa tcatacgcc aggaaactg cacatgcca gctctcaat ttaaacgag aaggaagcc acaacgccc gacgacgac ttggaatta agttcagag gacgaggct gtcgccagt gatttagac atgcacgcc ctcatcctg ggcggtttg caccaagac accgagccg cttaagacc gcagacgaa gtcatccag gagattgac gacatgatg caggaggag acgtgttcg ctagacggc tctccggga tccagggac tccataatt gagacgaat gagacgctg gaaaaggcg aaggaggtt ttaagctcg ccgttgtat gaagacaaa ttacgcgac ttgagtctg tcgcagtta aatgaatta tttctggaa ctagaggtt ttaattagg gaattttcc gaaacgctt atctcggag ttggccctc agagacgag ttggagtac gagaaggag ctgaagaac acttttatt tccctactt cttgccgtt caaaataaa cgtagacaa taccatgtt gagaagaag cggagttta aaaaatgcg tccaagact cccagcgga ctggacccg aaatattta accactgtc attccgtac catttagat agcggacca ccggataat caaacgtta caagtcctg attaaaatt ttgaaagcc ataaatgaa gacagcccc acagttccc actttatta acggactat atcctaaaa gttatatgt ccgacgtaa
- >TC032839 RA
cgcagtgtt gtgcaggct gtgagtgtg gagtggtgt ttcgcgatg cgggatttg gccacaaaa atggccgaa ctaaaattt gaagcacca ctggcccaa ttcgaggaa tcggatgaa tggggttcg accgagttt caaaacgcc accataaaa aacgagaac gaactgaac aacatcaac ctgaacaaa aaaatcaaa gacatcaac gacgtcctg aacgatttc aatgaggac atcctcaac aattcaaac accgaaacc ttgccgcca aaaaacgtg attttgaaa aacggtgaa acggcctcg gtggccgac aacttcacc gaaactttc tcgggctcg cttgaggac ctcgttaac acgttcgat gaaaaaatc accaagtgc ttcggcaac tacgaggag tcggtggag aagttggcc cccgtccag gtccggacg caggaggag atcatgaac gaatgccat tgtgcatgt tcgtgggca catcgcgtg ccttgtttg aaccaaact ggctctgtg cttacgtca aatgaggct aattacacc actaaagcc acctgttta atggccaat agtgtcatc ttgacaggt gaacggttg gagcctctc ggcgcgtcc gatgaccgc aaaagcgaa attgttcga aatagtgga tgcgacaaa tgtgtgtgt ttggaaaac gagaaagac ttcctccag acttcgcta tttacttta acgacgtac ttcgcccaa gtccagttc cggctgcgg caagtcgtc gacgcgccg ttcgaggac caagaagag ttgttaaaa tcgctggag gagtttgcg ttcactgat gtaaatctc cgggatagt aatgatagc ctcggtgac gcgttgctg gaaacggtc aaaaagcgg cgcgagcaa cagaaaatt ttgttggga cggttgaaa tcacagttg agacgatta aaagagttt gactggaac gaattagtg gaacatgta cggctaaag gaaaatttg gagcaacaa cttaagacg cagatagtt gatttggaa cggtttatt caatattta aggaacgtg gatactaaa tgtgtgtgt aaacggagt aggaaaagc ttcgggtcg tttctcgtc gaagccatc gatcggatt tttgcattt ttgcaaatg tttgccttg ctacagttt acttggggt tcgaattgc ttcgcctcg gaccggatc aaccaatgg gctgatctg agagccaga ctcgagctt gccataatc gacttgaag aaaacgttg cacagtaat gaagacgcg cacaaaatc acaacaatc gttcggagt aatttagcc acaagcatc aaagacttg atggagcac gggacaaag tcccacccg ttcctgggt tgtctgtcg catcaggca acacatggc tgggacatc atcctgttt tactaccat tttaaaaga ggtgtttca cccaaaact agcaagaaa aatttactg tgcgccgtc gaagagatc atttcgacg cattcgcgg tacaaaagg agccacgat tcgcacttt agagccttc gtttgtgcg gctctaaat gccaaaaga cttgtgccc tggttaaat ttaatcttc cactgtcgg cgggttatc gaaacattt tattatccg tggagctac gttgccacg accggatac caagacagt ctgaggagt ttggaggct ctgacaccg ctcaagttt gatctcccg gttgatttg gacgtgcga aagttccaa attgaggat aacccaaaa cccctagca tgtgggtcg gaacggctc ccagcaagg ccgtatgca aatggttac atcactctt tttattaca caaattgtc tcccgtatg aggtcaaga ctaacgagt cgttctttc agaatgtgg tggaccata acgggaaac ttcggcaac attctgccc attgactgg tccaaatca tacgccagg aaactgcac atgccagct ctcaattta aacgagaag gaagccaca acgcccgac gacgacttg gaattaagt tcagaggac gaggctgtc gccagtgat ttagacatg cacgccctc atcctgggc ggtttgcac caagacacc gagccgctt aagaccgca gacgaagtc atccaggag attgacgac atgatgcag gaggagacg tgttcgcta gacggctct ccgggatcc agggactcc ataattgag acgaatgag acgctggaa aaggcgaag gaggtttta agctcgccg ttgtatgaa gacaaatta cgcgacttg agtctgtcg cagttaaat gaattattt ctggaacta gaggtttta attagggaa ttttccgaa acgcttatc tcggagttg gccctcaga gacgagttg gagtacgag aaggagctg aagaacact tttatttcc ctacttctt gccgttcaa aataaacgt agacaatac catgttgag aagaagcgg agtttaaaa aatgcgtcc aagactccc agcggactg gacccgaaa tatttaacc actgtcatt ccgtaccat ttagatagc ggaccaccg gataatcaa acgttacaa gtcctgatt aaaattttg aaagccata aatgaagac agccccaca gttcccact ttattaacg gactatatc ctaaaagtt atatgtccg acgtaaagt agtcaagga agcacagat gagaaccca aaatctagc ttaaatata tttagtcta ggcttgaca tagcacaga tttcagttg caatattaa tttatttcc tggttgtta atattgttc ctctgtata tttttttaa gtgatgtgt atatatttt gcttgaaca ttctagtct aattatgta attgtaaag taacaatta aatgtgaac tattaataa gtattatgc acatctttg ctacagttc gtgcaatat gcactcgtt ttaatttta ctgatattt ttgtacaaa gtgatatta gctacgagt tttattctt gcccgactt gcgttttcg ctgatgaaa gtaatataa ggttaaatt aggtgaatt taacattta atgtcttaa atgttacct gtttcagag ccagccgtt gataatatt actgtagat ctcaatcaa ataaacctt tgcaattca aaatcacgt ttcttgcat atttttgta ggtcgtaaa ggtaacgag tgctttatg gcagagtct tgcaaattg atacaatct ggctgtatt tttttattt cgatttcaa tgttttggt ctgaataaa cctttattg tagaa
- >TC032839 RB
cgcagtgtt gtgcaggct gtgagtgtg gagtggtgt ttcgcgatg cgggatttg gccacaaaa atggccgaa ctaaaattt gaagcacca ctggcccaa ttcgaggaa tcggatgaa tggggttcg accgagttt caaaacgcc accataaaa aacgagaac gaactgaac aacatcaac ctgaacaaa aaaatcaaa gacatcaac gacgtcctg aacgatttc aatgaggac atcctcaac aattcaaac accgaaacc ttgccgcca aaaaacgtg attttgaaa aacggtgaa acggcctcg gtggccgac aacttcacc gaaactttc tcgggctcg cttgaggac ctcgttaac acgttcgat gaaaaaatc accaagtgc ttcggcaac tacgaggag tcggtggag aagttggcc cccgtccag gtccggacg caggaggag atcatgaac gaatgccat tgtgcatgt tcgtgggca catcgcgtg ccttgtttg aaccaaact ggctctgtg cttacgtca aatgaggct aattacacc actaaagcc acctgttta atggccaat agtgtcatc ttgacaggt gaacggttg gagcctctc ggcgcgtcc gatgaccgc aaaagcgaa attgttcga aatagtgga tgcgacaaa tgtgtgtgt ttggaaaac gagaaagac ttcctccag acttcgcta tttacttta acgacgtac ttcgcccaa gtccagttc cggctgcgg caagtcgtc gacgcgccg ttcgaggac caagaagag ttgttaaaa tcgctggag gagtttgcg ttcactgat gtaaatctc cgggatagt aatgatagc ctcggtgac gcgttgctg gaaacggtc aaaaagcgg cgcgagcaa cagaaaatt ttgttggga cggttgaaa tcacagttg agacgatta aaagagttt gactggaac gaattagtg gaacatgta cggctaaag gaaaatttg gagcaacaa cttaagacg cagatagtt gatttggaa cggtttatt caatattta aggaacgtg gatactaaa tgtgtgtgt aaacggagt aggaaaagc ttcgggtcg tttctcgtc gaagccatc gatcggatt tttgcattt ttgcaaatg tttgccttg ctacagttt acttggggt tcgaattgc ttcgcctcg gaccggatc aaccaatgg gctgatctg agagccaga ctcgagctt gccataatc gacttgaag aaaacgttg cacagtaat gaagacgcg cacaaaatc acaacaatc gttcggagt aatttagcc acaagcatc aaagacttg atggagcac gggacaaag tcccacccg ttcctgggt tgtctgtcg catcaggca acacatggc tgggacatc atcctgttt tactaccat tttaaaaga ggtgatgaa tacaactcg tcaacggtt cagaagtta tcgcgaagt ttcaatctt gcaattgca ggtgtttca cccaaaact agcaagaaa aatttactg tgcgccgtc gaagagatc atttcgacg cattcgcgg tacaaaagg agccacgat tcgcacttt agagccttc gtttgtgcg gctctaaat gccaaaaga cttgtgccc tggttaaat ttaatcttc cactgtcgg cgggttatc gaaacattt tattatccg tggagctac gttgccacg accggatac caagacagt ctgaggagt ttggaggct ctgacaccg ctcaagttt gatctcccg gttgatttg gacgtgcga aagttccaa attgaggat aacccaaaa cccctagca tgtgggtcg gaacggctc ccagcaagg ccgtatgca aatggttac atcactctt tttattaca caaattgtc tcccgtatg aggtcaaga ctaacgagt cgttctttc agaatgtgg tggaccata acgggaaac ttcggcaac attctgccc attgactgg tccaaatca tacgccagg aaactgcac atgccagct ctcaattta aacgagaag gaagccaca acgcccgac gacgacttg gaattaagt tcagaggac gaggctgtc gccagtgat ttagacatg cacgccctc atcctgggc ggtttgcac caagacacc gagccgctt aagaccgca gacgaagtc atccaggag attgacgac atgatgcag gaggagacg tgttcgcta gacggctct ccgggatcc agggactcc ataattgag acgaatgag acgctggaa aaggcgaag gaggtttta agctcgccg ttgtatgaa gacaaatta cgcgacttg agtctgtcg cagttaaat gaattattt ctggaacta gaggtttta attagggaa ttttccgaa acgcttatc tcggagttg gccctcaga gacgagttg gagtacgag aaggagctg aagaacact tttatttcc ctacttctt gccgttcaa aataaacgt agacaatac catgttgag aagaagcgg agtttaaaa aatgcgtcc aagactccc agcggactg gacccgaaa tatttaacc actgtcatt ccgtaccat ttagatagc ggaccaccg gataatcaa acgttacaa gtcctgatt aaaattttg aaagccata aatgaagac agccccaca gttcccact ttattaacg gactatatc ctaaaagtt atatgtccg acgtaaagt agtcaagga agcacagat gagaaccca aaatctagc ttaaatata tttagtcta ggcttgaca tagcacaga tttcagttg caatattaa tttatttcc tggttgtta atattgttc ctctgtata tttttttaa gtgatgtgt atatatttt gcttgaaca ttctagtct aattatgta attgtaaag taacaatta aatgtgaac tattaataa gtattatgc acatctttg ctacagttc gtgcaatat gcactcgtt ttaatttta ctgatattt ttgtacaaa gtgatatta gctacgagt tttattctt gcccgactt gcgttttcg ctgatgaaa gtaatataa ggttaaatt aggtgaatt taacattta atgtcttaa atgttacct gtttcagag ccagccgtt gataatatt actgtagat ctcaatcaa ataaacctt tgcaattca aaatcacgt ttcttgcat atttttgta ggtcgtaaa ggtaacgag tgctttatg gcagagtct tgcaaattg atacaatct ggctgtatt tttttattt cgatttcaa tgttttggt ctgaataaa cctttattg tagaa
