Status
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Gene information
General information
TC032839
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007278,TC007279,TC007280
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 6593331..6604104
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032839
    iBeetle screen iB_01159
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%
    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature coxal musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Adult morphology
    adult appendages distal pigmentation/tanning decreased - Number of animals showing the phenotype: 5


    elytra blistered - Number of animals showing the phenotype: 2


    wings not closed - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032839 RA
      MRDLATKMA ELKFEAPLA QFEESDEWG STEFQNATI 
      KNENELNNI NLNKKIKDI NDVLNDFNE DILNNSNTE 
      TLPPKNVIL KNGETASVA DNFTETFSG SLEDLVNTF 
      DEKITKCFG NYEESVEKL APVQVRTQE EIMNECHCA 
      CSWAHRVPC LNQTGSVLT SNEANYTTK ATCLMANSV 
      ILTGERLEP LGASDDRKS EIVRNSGCD KCVCLENEK 
      DFLQTSLFT LTTYFAQVQ FRLRQVVDA PFEDQEELL 
      KSLEEFAFT DVNLRDSND SLGDALLET VKKRREQQK 
      ILLGRLKSQ LRRLKEFDW NELVEHVRL KENLEQQLK 
      TQIVDLERF IQYLRNVDT KCVCKRSRK SFGSFLVEA 
      IDRIFAFLQ MFALLQFTW GSNCFASDR INQWADLRA 
      RLELAIIDL KKTLHSNED AHKITTIVR SNLATSIKD 
      LMEHGTKSH PFLGCLSHQ ATHGWDIIL FYYHFKRGV 
      SPKTSKKNL LCAVEEIIS THSRYKRSH DSHFRAFVC 
      AALNAKRLV PWLNLIFHC RRVIETFYY PWSYVATTG 
      YQDSLRSLE ALTPLKFDL PVDLDVRKF QIEDNPKPL 
      ACGSERLPA RPYANGYIT LFITQIVSR MRSRLTSRS 
      FRMWWTITG NFGNILPID WSKSYARKL HMPALNLNE 
      KEATTPDDD LELSSEDEA VASDLDMHA LILGGLHQD 
      TEPLKTADE VIQEIDDMM QEETCSLDG SPGSRDSII 
      ETNETLEKA KEVLSSPLY EDKLRDLSL SQLNELFLE 
      LEVLIREFS ETLISELAL RDELEYEKE LKNTFISLL 
      LAVQNKRRQ YHVEKKRSL KNASKTPSG LDPKYLTTV 
      IPYHLDSGP PDNQTLQVL IKILKAINE DSPTVPTLL 
      TDYILKVIC PT
    • >TC032839 RB
      MRDLATKMA ELKFEAPLA QFEESDEWG STEFQNATI 
      KNENELNNI NLNKKIKDI NDVLNDFNE DILNNSNTE 
      TLPPKNVIL KNGETASVA DNFTETFSG SLEDLVNTF 
      DEKITKCFG NYEESVEKL APVQVRTQE EIMNECHCA 
      CSWAHRVPC LNQTGSVLT SNEANYTTK ATCLMANSV 
      ILTGERLEP LGASDDRKS EIVRNSGCD KCVCLENEK 
      DFLQTSLFT LTTYFAQVQ FRLRQVVDA PFEDQEELL 
      KSLEEFAFT DVNLRDSND SLGDALLET VKKRREQQK 
      ILLGRLKSQ LRRLKEFDW NELVEHVRL KENLEQQLK 
      TQIVDLERF IQYLRNVDT KCVCKRSRK SFGSFLVEA 
      IDRIFAFLQ MFALLQFTW GSNCFASDR INQWADLRA 
      RLELAIIDL KKTLHSNED AHKITTIVR SNLATSIKD 
      LMEHGTKSH PFLGCLSHQ ATHGWDIIL FYYHFKRGD 
      EYNSSTVQK LSRSFNLAI AGVSPKTSK KNLLCAVEE 
      IISTHSRYK RSHDSHFRA FVCAALNAK RLVPWLNLI 
      FHCRRVIET FYYPWSYVA TTGYQDSLR SLEALTPLK 
      FDLPVDLDV RKFQIEDNP KPLACGSER LPARPYANG 
      YITLFITQI VSRMRSRLT SRSFRMWWT ITGNFGNIL 
      PIDWSKSYA RKLHMPALN LNEKEATTP DDDLELSSE 
      DEAVASDLD MHALILGGL HQDTEPLKT ADEVIQEID 
      DMMQEETCS LDGSPGSRD SIIETNETL EKAKEVLSS 
      PLYEDKLRD LSLSQLNEL FLELEVLIR EFSETLISE 
      LALRDELEY EKELKNTFI SLLLAVQNK RRQYHVEKK 
      RSLKNASKT PSGLDPKYL TTVIPYHLD SGPPDNQTL 
      QVLIKILKA INEDSPTVP TLLTDYILK VICPT
    CDS sequence
    • >TC032839 RA
      atgcgggat ttggccaca aaaatggcc gaactaaaa 
      tttgaagca ccactggcc caattcgag gaatcggat 
      gaatggggt tcgaccgag tttcaaaac gccaccata 
      aaaaacgag aacgaactg aacaacatc aacctgaac 
      aaaaaaatc aaagacatc aacgacgtc ctgaacgat 
      ttcaatgag gacatcctc aacaattca aacaccgaa 
      accttgccg ccaaaaaac gtgattttg aaaaacggt 
      gaaacggcc tcggtggcc gacaacttc accgaaact 
      ttctcgggc tcgcttgag gacctcgtt aacacgttc 
      gatgaaaaa atcaccaag tgcttcggc aactacgag 
      gagtcggtg gagaagttg gcccccgtc caggtccgg 
      acgcaggag gagatcatg aacgaatgc cattgtgca 
      tgttcgtgg gcacatcgc gtgccttgt ttgaaccaa 
      actggctct gtgcttacg tcaaatgag gctaattac 
      accactaaa gccacctgt ttaatggcc aatagtgtc 
      atcttgaca ggtgaacgg ttggagcct ctcggcgcg 
      tccgatgac cgcaaaagc gaaattgtt cgaaatagt 
      ggatgcgac aaatgtgtg tgtttggaa aacgagaaa 
      gacttcctc cagacttcg ctatttact ttaacgacg 
      tacttcgcc caagtccag ttccggctg cggcaagtc 
      gtcgacgcg ccgttcgag gaccaagaa gagttgtta 
      aaatcgctg gaggagttt gcgttcact gatgtaaat 
      ctccgggat agtaatgat agcctcggt gacgcgttg 
      ctggaaacg gtcaaaaag cggcgcgag caacagaaa 
      attttgttg ggacggttg aaatcacag ttgagacga 
      ttaaaagag tttgactgg aacgaatta gtggaacat 
      gtacggcta aaggaaaat ttggagcaa caacttaag 
      acgcagata gttgatttg gaacggttt attcaatat 
      ttaaggaac gtggatact aaatgtgtg tgtaaacgg 
      agtaggaaa agcttcggg tcgtttctc gtcgaagcc 
      atcgatcgg atttttgca tttttgcaa atgtttgcc 
      ttgctacag tttacttgg ggttcgaat tgcttcgcc 
      tcggaccgg atcaaccaa tgggctgat ctgagagcc 
      agactcgag cttgccata atcgacttg aagaaaacg 
      ttgcacagt aatgaagac gcgcacaaa atcacaaca 
      atcgttcgg agtaattta gccacaagc atcaaagac 
      ttgatggag cacgggaca aagtcccac ccgttcctg 
      ggttgtctg tcgcatcag gcaacacat ggctgggac 
      atcatcctg ttttactac cattttaaa agaggtgtt 
      tcacccaaa actagcaag aaaaattta ctgtgcgcc 
      gtcgaagag atcatttcg acgcattcg cggtacaaa 
      aggagccac gattcgcac tttagagcc ttcgtttgt 
      gcggctcta aatgccaaa agacttgtg ccctggtta 
      aatttaatc ttccactgt cggcgggtt atcgaaaca 
      ttttattat ccgtggagc tacgttgcc acgaccgga 
      taccaagac agtctgagg agtttggag gctctgaca 
      ccgctcaag tttgatctc ccggttgat ttggacgtg 
      cgaaagttc caaattgag gataaccca aaaccccta 
      gcatgtggg tcggaacgg ctcccagca aggccgtat 
      gcaaatggt tacatcact ctttttatt acacaaatt 
      gtctcccgt atgaggtca agactaacg agtcgttct 
      ttcagaatg tggtggacc ataacggga aacttcggc 
      aacattctg cccattgac tggtccaaa tcatacgcc 
      aggaaactg cacatgcca gctctcaat ttaaacgag 
      aaggaagcc acaacgccc gacgacgac ttggaatta 
      agttcagag gacgaggct gtcgccagt gatttagac 
      atgcacgcc ctcatcctg ggcggtttg caccaagac 
      accgagccg cttaagacc gcagacgaa gtcatccag 
      gagattgac gacatgatg caggaggag acgtgttcg 
      ctagacggc tctccggga tccagggac tccataatt 
      gagacgaat gagacgctg gaaaaggcg aaggaggtt 
      ttaagctcg ccgttgtat gaagacaaa ttacgcgac 
      ttgagtctg tcgcagtta aatgaatta tttctggaa 
      ctagaggtt ttaattagg gaattttcc gaaacgctt 
      atctcggag ttggccctc agagacgag ttggagtac 
      gagaaggag ctgaagaac acttttatt tccctactt 
      cttgccgtt caaaataaa cgtagacaa taccatgtt 
      gagaagaag cggagttta aaaaatgcg tccaagact 
      cccagcgga ctggacccg aaatattta accactgtc 
      attccgtac catttagat agcggacca ccggataat 
      caaacgtta caagtcctg attaaaatt ttgaaagcc 
      ataaatgaa gacagcccc acagttccc actttatta 
      acggactat atcctaaaa gttatatgt ccgacgtaa
    • >TC032839 RB
      atgcgggat ttggccaca aaaatggcc gaactaaaa 
      tttgaagca ccactggcc caattcgag gaatcggat 
      gaatggggt tcgaccgag tttcaaaac gccaccata 
      aaaaacgag aacgaactg aacaacatc aacctgaac 
      aaaaaaatc aaagacatc aacgacgtc ctgaacgat 
      ttcaatgag gacatcctc aacaattca aacaccgaa 
      accttgccg ccaaaaaac gtgattttg aaaaacggt 
      gaaacggcc tcggtggcc gacaacttc accgaaact 
      ttctcgggc tcgcttgag gacctcgtt aacacgttc 
      gatgaaaaa atcaccaag tgcttcggc aactacgag 
      gagtcggtg gagaagttg gcccccgtc caggtccgg 
      acgcaggag gagatcatg aacgaatgc cattgtgca 
      tgttcgtgg gcacatcgc gtgccttgt ttgaaccaa 
      actggctct gtgcttacg tcaaatgag gctaattac 
      accactaaa gccacctgt ttaatggcc aatagtgtc 
      atcttgaca ggtgaacgg ttggagcct ctcggcgcg 
      tccgatgac cgcaaaagc gaaattgtt cgaaatagt 
      ggatgcgac aaatgtgtg tgtttggaa aacgagaaa 
      gacttcctc cagacttcg ctatttact ttaacgacg 
      tacttcgcc caagtccag ttccggctg cggcaagtc 
      gtcgacgcg ccgttcgag gaccaagaa gagttgtta 
      aaatcgctg gaggagttt gcgttcact gatgtaaat 
      ctccgggat agtaatgat agcctcggt gacgcgttg 
      ctggaaacg gtcaaaaag cggcgcgag caacagaaa 
      attttgttg ggacggttg aaatcacag ttgagacga 
      ttaaaagag tttgactgg aacgaatta gtggaacat 
      gtacggcta aaggaaaat ttggagcaa caacttaag 
      acgcagata gttgatttg gaacggttt attcaatat 
      ttaaggaac gtggatact aaatgtgtg tgtaaacgg 
      agtaggaaa agcttcggg tcgtttctc gtcgaagcc 
      atcgatcgg atttttgca tttttgcaa atgtttgcc 
      ttgctacag tttacttgg ggttcgaat tgcttcgcc 
      tcggaccgg atcaaccaa tgggctgat ctgagagcc 
      agactcgag cttgccata atcgacttg aagaaaacg 
      ttgcacagt aatgaagac gcgcacaaa atcacaaca 
      atcgttcgg agtaattta gccacaagc atcaaagac 
      ttgatggag cacgggaca aagtcccac ccgttcctg 
      ggttgtctg tcgcatcag gcaacacat ggctgggac 
      atcatcctg ttttactac cattttaaa agaggtgat 
      gaatacaac tcgtcaacg gttcagaag ttatcgcga 
      agtttcaat cttgcaatt gcaggtgtt tcacccaaa 
      actagcaag aaaaattta ctgtgcgcc gtcgaagag 
      atcatttcg acgcattcg cggtacaaa aggagccac 
      gattcgcac tttagagcc ttcgtttgt gcggctcta 
      aatgccaaa agacttgtg ccctggtta aatttaatc 
      ttccactgt cggcgggtt atcgaaaca ttttattat 
      ccgtggagc tacgttgcc acgaccgga taccaagac 
      agtctgagg agtttggag gctctgaca ccgctcaag 
      tttgatctc ccggttgat ttggacgtg cgaaagttc 
      caaattgag gataaccca aaaccccta gcatgtggg 
      tcggaacgg ctcccagca aggccgtat gcaaatggt 
      tacatcact ctttttatt acacaaatt gtctcccgt 
      atgaggtca agactaacg agtcgttct ttcagaatg 
      tggtggacc ataacggga aacttcggc aacattctg 
      cccattgac tggtccaaa tcatacgcc aggaaactg 
      cacatgcca gctctcaat ttaaacgag aaggaagcc 
      acaacgccc gacgacgac ttggaatta agttcagag 
      gacgaggct gtcgccagt gatttagac atgcacgcc 
      ctcatcctg ggcggtttg caccaagac accgagccg 
      cttaagacc gcagacgaa gtcatccag gagattgac 
      gacatgatg caggaggag acgtgttcg ctagacggc 
      tctccggga tccagggac tccataatt gagacgaat 
      gagacgctg gaaaaggcg aaggaggtt ttaagctcg 
      ccgttgtat gaagacaaa ttacgcgac ttgagtctg 
      tcgcagtta aatgaatta tttctggaa ctagaggtt 
      ttaattagg gaattttcc gaaacgctt atctcggag 
      ttggccctc agagacgag ttggagtac gagaaggag 
      ctgaagaac acttttatt tccctactt cttgccgtt 
      caaaataaa cgtagacaa taccatgtt gagaagaag 
      cggagttta aaaaatgcg tccaagact cccagcgga 
      ctggacccg aaatattta accactgtc attccgtac 
      catttagat agcggacca ccggataat caaacgtta 
      caagtcctg attaaaatt ttgaaagcc ataaatgaa 
      gacagcccc acagttccc actttatta acggactat 
      atcctaaaa gttatatgt ccgacgtaa
    mRNA sequence
    • >TC032839 RA
      cgcagtgtt gtgcaggct gtgagtgtg gagtggtgt 
      ttcgcgatg cgggatttg gccacaaaa atggccgaa 
      ctaaaattt gaagcacca ctggcccaa ttcgaggaa 
      tcggatgaa tggggttcg accgagttt caaaacgcc 
      accataaaa aacgagaac gaactgaac aacatcaac 
      ctgaacaaa aaaatcaaa gacatcaac gacgtcctg 
      aacgatttc aatgaggac atcctcaac aattcaaac 
      accgaaacc ttgccgcca aaaaacgtg attttgaaa 
      aacggtgaa acggcctcg gtggccgac aacttcacc 
      gaaactttc tcgggctcg cttgaggac ctcgttaac 
      acgttcgat gaaaaaatc accaagtgc ttcggcaac 
      tacgaggag tcggtggag aagttggcc cccgtccag 
      gtccggacg caggaggag atcatgaac gaatgccat 
      tgtgcatgt tcgtgggca catcgcgtg ccttgtttg 
      aaccaaact ggctctgtg cttacgtca aatgaggct 
      aattacacc actaaagcc acctgttta atggccaat 
      agtgtcatc ttgacaggt gaacggttg gagcctctc 
      ggcgcgtcc gatgaccgc aaaagcgaa attgttcga 
      aatagtgga tgcgacaaa tgtgtgtgt ttggaaaac 
      gagaaagac ttcctccag acttcgcta tttacttta 
      acgacgtac ttcgcccaa gtccagttc cggctgcgg 
      caagtcgtc gacgcgccg ttcgaggac caagaagag 
      ttgttaaaa tcgctggag gagtttgcg ttcactgat 
      gtaaatctc cgggatagt aatgatagc ctcggtgac 
      gcgttgctg gaaacggtc aaaaagcgg cgcgagcaa 
      cagaaaatt ttgttggga cggttgaaa tcacagttg 
      agacgatta aaagagttt gactggaac gaattagtg 
      gaacatgta cggctaaag gaaaatttg gagcaacaa 
      cttaagacg cagatagtt gatttggaa cggtttatt 
      caatattta aggaacgtg gatactaaa tgtgtgtgt 
      aaacggagt aggaaaagc ttcgggtcg tttctcgtc 
      gaagccatc gatcggatt tttgcattt ttgcaaatg 
      tttgccttg ctacagttt acttggggt tcgaattgc 
      ttcgcctcg gaccggatc aaccaatgg gctgatctg 
      agagccaga ctcgagctt gccataatc gacttgaag 
      aaaacgttg cacagtaat gaagacgcg cacaaaatc 
      acaacaatc gttcggagt aatttagcc acaagcatc 
      aaagacttg atggagcac gggacaaag tcccacccg 
      ttcctgggt tgtctgtcg catcaggca acacatggc 
      tgggacatc atcctgttt tactaccat tttaaaaga 
      ggtgtttca cccaaaact agcaagaaa aatttactg 
      tgcgccgtc gaagagatc atttcgacg cattcgcgg 
      tacaaaagg agccacgat tcgcacttt agagccttc 
      gtttgtgcg gctctaaat gccaaaaga cttgtgccc 
      tggttaaat ttaatcttc cactgtcgg cgggttatc 
      gaaacattt tattatccg tggagctac gttgccacg 
      accggatac caagacagt ctgaggagt ttggaggct 
      ctgacaccg ctcaagttt gatctcccg gttgatttg 
      gacgtgcga aagttccaa attgaggat aacccaaaa 
      cccctagca tgtgggtcg gaacggctc ccagcaagg 
      ccgtatgca aatggttac atcactctt tttattaca 
      caaattgtc tcccgtatg aggtcaaga ctaacgagt 
      cgttctttc agaatgtgg tggaccata acgggaaac 
      ttcggcaac attctgccc attgactgg tccaaatca 
      tacgccagg aaactgcac atgccagct ctcaattta 
      aacgagaag gaagccaca acgcccgac gacgacttg 
      gaattaagt tcagaggac gaggctgtc gccagtgat 
      ttagacatg cacgccctc atcctgggc ggtttgcac 
      caagacacc gagccgctt aagaccgca gacgaagtc 
      atccaggag attgacgac atgatgcag gaggagacg 
      tgttcgcta gacggctct ccgggatcc agggactcc 
      ataattgag acgaatgag acgctggaa aaggcgaag 
      gaggtttta agctcgccg ttgtatgaa gacaaatta 
      cgcgacttg agtctgtcg cagttaaat gaattattt 
      ctggaacta gaggtttta attagggaa ttttccgaa 
      acgcttatc tcggagttg gccctcaga gacgagttg 
      gagtacgag aaggagctg aagaacact tttatttcc 
      ctacttctt gccgttcaa aataaacgt agacaatac 
      catgttgag aagaagcgg agtttaaaa aatgcgtcc 
      aagactccc agcggactg gacccgaaa tatttaacc 
      actgtcatt ccgtaccat ttagatagc ggaccaccg 
      gataatcaa acgttacaa gtcctgatt aaaattttg 
      aaagccata aatgaagac agccccaca gttcccact 
      ttattaacg gactatatc ctaaaagtt atatgtccg 
      acgtaaagt agtcaagga agcacagat gagaaccca 
      aaatctagc ttaaatata tttagtcta ggcttgaca 
      tagcacaga tttcagttg caatattaa tttatttcc 
      tggttgtta atattgttc ctctgtata tttttttaa 
      gtgatgtgt atatatttt gcttgaaca ttctagtct 
      aattatgta attgtaaag taacaatta aatgtgaac 
      tattaataa gtattatgc acatctttg ctacagttc 
      gtgcaatat gcactcgtt ttaatttta ctgatattt 
      ttgtacaaa gtgatatta gctacgagt tttattctt 
      gcccgactt gcgttttcg ctgatgaaa gtaatataa 
      ggttaaatt aggtgaatt taacattta atgtcttaa 
      atgttacct gtttcagag ccagccgtt gataatatt 
      actgtagat ctcaatcaa ataaacctt tgcaattca 
      aaatcacgt ttcttgcat atttttgta ggtcgtaaa 
      ggtaacgag tgctttatg gcagagtct tgcaaattg 
      atacaatct ggctgtatt tttttattt cgatttcaa 
      tgttttggt ctgaataaa cctttattg tagaa
    • >TC032839 RB
      cgcagtgtt gtgcaggct gtgagtgtg gagtggtgt 
      ttcgcgatg cgggatttg gccacaaaa atggccgaa 
      ctaaaattt gaagcacca ctggcccaa ttcgaggaa 
      tcggatgaa tggggttcg accgagttt caaaacgcc 
      accataaaa aacgagaac gaactgaac aacatcaac 
      ctgaacaaa aaaatcaaa gacatcaac gacgtcctg 
      aacgatttc aatgaggac atcctcaac aattcaaac 
      accgaaacc ttgccgcca aaaaacgtg attttgaaa 
      aacggtgaa acggcctcg gtggccgac aacttcacc 
      gaaactttc tcgggctcg cttgaggac ctcgttaac 
      acgttcgat gaaaaaatc accaagtgc ttcggcaac 
      tacgaggag tcggtggag aagttggcc cccgtccag 
      gtccggacg caggaggag atcatgaac gaatgccat 
      tgtgcatgt tcgtgggca catcgcgtg ccttgtttg 
      aaccaaact ggctctgtg cttacgtca aatgaggct 
      aattacacc actaaagcc acctgttta atggccaat 
      agtgtcatc ttgacaggt gaacggttg gagcctctc 
      ggcgcgtcc gatgaccgc aaaagcgaa attgttcga 
      aatagtgga tgcgacaaa tgtgtgtgt ttggaaaac 
      gagaaagac ttcctccag acttcgcta tttacttta 
      acgacgtac ttcgcccaa gtccagttc cggctgcgg 
      caagtcgtc gacgcgccg ttcgaggac caagaagag 
      ttgttaaaa tcgctggag gagtttgcg ttcactgat 
      gtaaatctc cgggatagt aatgatagc ctcggtgac 
      gcgttgctg gaaacggtc aaaaagcgg cgcgagcaa 
      cagaaaatt ttgttggga cggttgaaa tcacagttg 
      agacgatta aaagagttt gactggaac gaattagtg 
      gaacatgta cggctaaag gaaaatttg gagcaacaa 
      cttaagacg cagatagtt gatttggaa cggtttatt 
      caatattta aggaacgtg gatactaaa tgtgtgtgt 
      aaacggagt aggaaaagc ttcgggtcg tttctcgtc 
      gaagccatc gatcggatt tttgcattt ttgcaaatg 
      tttgccttg ctacagttt acttggggt tcgaattgc 
      ttcgcctcg gaccggatc aaccaatgg gctgatctg 
      agagccaga ctcgagctt gccataatc gacttgaag 
      aaaacgttg cacagtaat gaagacgcg cacaaaatc 
      acaacaatc gttcggagt aatttagcc acaagcatc 
      aaagacttg atggagcac gggacaaag tcccacccg 
      ttcctgggt tgtctgtcg catcaggca acacatggc 
      tgggacatc atcctgttt tactaccat tttaaaaga 
      ggtgatgaa tacaactcg tcaacggtt cagaagtta 
      tcgcgaagt ttcaatctt gcaattgca ggtgtttca 
      cccaaaact agcaagaaa aatttactg tgcgccgtc 
      gaagagatc atttcgacg cattcgcgg tacaaaagg 
      agccacgat tcgcacttt agagccttc gtttgtgcg 
      gctctaaat gccaaaaga cttgtgccc tggttaaat 
      ttaatcttc cactgtcgg cgggttatc gaaacattt 
      tattatccg tggagctac gttgccacg accggatac 
      caagacagt ctgaggagt ttggaggct ctgacaccg 
      ctcaagttt gatctcccg gttgatttg gacgtgcga 
      aagttccaa attgaggat aacccaaaa cccctagca 
      tgtgggtcg gaacggctc ccagcaagg ccgtatgca 
      aatggttac atcactctt tttattaca caaattgtc 
      tcccgtatg aggtcaaga ctaacgagt cgttctttc 
      agaatgtgg tggaccata acgggaaac ttcggcaac 
      attctgccc attgactgg tccaaatca tacgccagg 
      aaactgcac atgccagct ctcaattta aacgagaag 
      gaagccaca acgcccgac gacgacttg gaattaagt 
      tcagaggac gaggctgtc gccagtgat ttagacatg 
      cacgccctc atcctgggc ggtttgcac caagacacc 
      gagccgctt aagaccgca gacgaagtc atccaggag 
      attgacgac atgatgcag gaggagacg tgttcgcta 
      gacggctct ccgggatcc agggactcc ataattgag 
      acgaatgag acgctggaa aaggcgaag gaggtttta 
      agctcgccg ttgtatgaa gacaaatta cgcgacttg 
      agtctgtcg cagttaaat gaattattt ctggaacta 
      gaggtttta attagggaa ttttccgaa acgcttatc 
      tcggagttg gccctcaga gacgagttg gagtacgag 
      aaggagctg aagaacact tttatttcc ctacttctt 
      gccgttcaa aataaacgt agacaatac catgttgag 
      aagaagcgg agtttaaaa aatgcgtcc aagactccc 
      agcggactg gacccgaaa tatttaacc actgtcatt 
      ccgtaccat ttagatagc ggaccaccg gataatcaa 
      acgttacaa gtcctgatt aaaattttg aaagccata 
      aatgaagac agccccaca gttcccact ttattaacg 
      gactatatc ctaaaagtt atatgtccg acgtaaagt 
      agtcaagga agcacagat gagaaccca aaatctagc 
      ttaaatata tttagtcta ggcttgaca tagcacaga 
      tttcagttg caatattaa tttatttcc tggttgtta 
      atattgttc ctctgtata tttttttaa gtgatgtgt 
      atatatttt gcttgaaca ttctagtct aattatgta 
      attgtaaag taacaatta aatgtgaac tattaataa 
      gtattatgc acatctttg ctacagttc gtgcaatat 
      gcactcgtt ttaatttta ctgatattt ttgtacaaa 
      gtgatatta gctacgagt tttattctt gcccgactt 
      gcgttttcg ctgatgaaa gtaatataa ggttaaatt 
      aggtgaatt taacattta atgtcttaa atgttacct 
      gtttcagag ccagccgtt gataatatt actgtagat 
      ctcaatcaa ataaacctt tgcaattca aaatcacgt 
      ttcttgcat atttttgta ggtcgtaaa ggtaacgag 
      tgctttatg gcagagtct tgcaaattg atacaatct 
      ggctgtatt tttttattt cgatttcaa tgttttggt 
      ctgaataaa cctttattg tagaa