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Gene information
General information
TC007229 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7197992..7200501
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007229
    iBeetle screen iB_01152

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007229 RA
      MAAGTLYTY PDNFRAAKA LIAAQYSKS NVKVSPNFV 
      FGETNKSKE FLKKFPSGK VPAFESNDG KYLQDSNAI 
      AYYVANEQL RGKSEYEKA QILQWVGFG ESEILPAGC 
      AWVFPILGV VKKNAATEE GYARAKEDM RAALAILDS 
      HLLTRTYLV GERITLADI VVACNLSNL YKYAFDPDF 
      RKPFVNVNR WFTTLINQP QFKAVLGQV QFLDKVAQP 
      FTGAQGGEG KNKKKEEKK EKKQQQQPK KETPKKEKE 
      PVEEMDAAE AALAAEPKS KDPFESMPK GTFNMDDFK 
      RFYSNEDEA KSIPYFWEK FDPENYSIW YGEYKYPEE 
      LTKIFMSCN LITGMYQRL DKMRKQAFA SVCLFGEDN 
      NSSISGIWV WRGHDLAFT LSPDWQIDY ESYDWKKLD 
      AKSEETKKL VAQYFSWTG TDKKGRKFN QGKIFK
    CDS sequence
    • >TC007229 RA
      atggctgca ggaacactg tacacctat cccgacaat 
      ttccgtgct gctaaggct ttaatcgca gcccagtac 
      tccaaaagc aatgttaaa gtaagccca aactttgtg 
      ttcggtgaa accaacaag tcgaaagaa ttcttaaag 
      aaatttccc agtggaaag gtgccagct ttcgagtca 
      aacgatggc aagtatctg caagacagc aacgcaatt 
      gcctattat gtcgccaat gaacaattg agaggcaag 
      tcggagtac gagaaagct cagattttg caatgggtg 
      ggctttgga gagtcggag atactccca gctggctgt 
      gcctgggtg ttccccatt ttgggggtt gtcaagaag 
      aacgccgcc actgaggag ggatatgca agagccaag 
      gaggacatg agggctgct ctggcaatc ctagacagc 
      catttgctt acccgtact tatctagtt ggggaacgt 
      atcacttta gctgatatt gttgtagct tgcaatctt 
      tcaaattta tacaaatat gcgttcgat cccgacttt 
      agaaaacca tttgtcaac gttaacaga tggttcaca 
      actttaatc aaccaacct cagtttaag gccgttttg 
      ggccaggtg caatttttg gacaaagtt gcacaacct 
      ttcaccggc gcgcaggga ggtgaaggt aagaacaaa 
      aagaaagag gagaaaaaa gagaagaag cagcagcag 
      cagcctaaa aaggaaaca ccaaaaaag gagaaagag 
      cccgttgaa gaaatggat gcggctgaa gcagcgctc 
      gctgccgag cccaaatcg aaggacccc tttgaatcc 
      atgcccaaa gggacgttc aatatggac gatttcaag 
      agattttat tctaacgaa gatgaagcc aaatcaatt 
      ccgtatttc tgggagaag ttcgatcct gaaaactac 
      tctatttgg tacggggaa tataagtac cctgaagag 
      ctcactaag atttttatg agctgcaat ttgatcacc 
      ggaatgtat cagagattg gataaaatg cgcaaacaa 
      gcctttgcc tcagtttgt ctttttggt gaggacaat 
      aacagttca atctccggg atttgggtg tggaggggc 
      cacgacctc gcattcact ttgagtcct gactggcaa 
      atcgactac gagtcgtat gattggaag aaattggat 
      gcaaaatca gaagagact aaaaaatta gttgcccaa 
      tacttctca tggactggc actgacaaa aagggaagg 
      aagtttaac cagggcaaa atctttaag taa
    mRNA sequence
    • >TC007229 RA
      atcctttag ggcagaaag tctaagtac ggttctgtt 
      atttttagt gggcctttt taaagaaaa aaaataatt 
      tgtataatt cctggaaga tgtgtgcaa tttagtgtt 
      gttttttgc acaaaagcg ggcatttcg aggctgtaa 
      aaatcgaaa aaaaaaggc ctaagcccg acacgtcac 
      cagaccgca agcccaaaa ttccctatg cagctgtca 
      aactcttca agtcctttt ctgtgagaa attggacga 
      gcaatggct gcaggaaca ctgtacacc tatcccgac 
      aatttccgt gctgctaag gctttaatc gcagcccag 
      tactccaaa agcaatgtt aaagtaagc ccaaacttt 
      gtgttcggt gaaaccaac aagtcgaaa gaattctta 
      aagaaattt cccagtgga aaggtgcca gctttcgag 
      tcaaacgat ggcaagtat ctgcaagac agcaacgca 
      attgcctat tatgtcgcc aatgaacaa ttgagaggc 
      aagtcggag tacgagaaa gctcagatt ttgcaatgg 
      gtgggcttt ggagagtcg gagatactc ccagctggc 
      tgtgcctgg gtgttcccc attttgggg gttgtcaag 
      aagaacgcc gccactgag gagggatat gcaagagcc 
      aaggaggac atgagggct gctctggca atcctagac 
      agccatttg cttacccgt acttatcta gttggggaa 
      cgtatcact ttagctgat attgttgta gcttgcaat 
      ctttcaaat ttatacaaa tatgcgttc gatcccgac 
      tttagaaaa ccatttgtc aacgttaac agatggttc 
      acaacttta atcaaccaa cctcagttt aaggccgtt 
      ttgggccag gtgcaattt ttggacaaa gttgcacaa 
      cctttcacc ggcgcgcag ggaggtgaa ggtaagaac 
      aaaaagaaa gaggagaaa aaagagaag aagcagcag 
      cagcagcct aaaaaggaa acaccaaaa aaggagaaa 
      gagcccgtt gaagaaatg gatgcggct gaagcagcg 
      ctcgctgcc gagcccaaa tcgaaggac ccctttgaa 
      tccatgccc aaagggacg ttcaatatg gacgatttc 
      aagagattt tattctaac gaagatgaa gccaaatca 
      attccgtat ttctgggag aagttcgat cctgaaaac 
      tactctatt tggtacggg gaatataag taccctgaa 
      gagctcact aagattttt atgagctgc aatttgatc 
      accggaatg tatcagaga ttggataaa atgcgcaaa 
      caagccttt gcctcagtt tgtcttttt ggtgaggac 
      aataacagt tcaatctcc gggatttgg gtgtggagg 
      ggccacgac ctcgcattc actttgagt cctgactgg 
      caaatcgac tacgagtcg tatgattgg aagaaattg 
      gatgcaaaa tcagaagag actaaaaaa ttagttgcc 
      caatacttc tcatggact ggcactgac aaaaaggga 
      aggaagttt aaccagggc aaaatcttt aagtaaaac 
      ccgaggtgt aatatattt tttttacaa cc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY