Status
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Gene information
General information
TC007228 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7226368..7228081
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007228
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01151

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 1

    vitellarium size decreased - Number of animals showing the phenotype: 1
    vitellogenic egg chamber degenerated - Number of animals showing the phenotype: 3

    vitellarium potentially size increased - Number of animals showing the phenotype: 2

    lateral oviduct necrotic - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007228 RA
      MEQKQTDVH LRAMLNSPA RFTPAVPTP 
      DWSAGPAVP PSPQIPGKS NMIVATPGS 
      AAGEPHTNI TIQNCVTTV DLNTKLDLA 
      LINARTRNS EYNPARFHG LIMRLRDPR 
      TTALIFQTG KIVCTGAKS EQDALLASK 
      KFARIIQKL GFDIKFDNF KIQNLVATC 
      DLRFPIKLE SLSLLHGQF CSYEPELFP 
      GIIYRMIKP HLCLLIFVN GKIVFTGSK 
      SREGIKESL DNIYPILRS FKKN
    • >TC007228 RB
      MEQKQTDVH LRAMLNSPA RFTPAVPTP 
      DWSAGPAVP PSIPGKSNM IVATPGSAA 
      GEPHTNITI QNCVTTVDL NTKLDLALI 
      NARTRNSEY NPARFHGLI MRLRDPRTT 
      ALIFQTGKI VCTGAKSEQ DALLASKKF 
      ARIIQKLGF DIKFDNFKI QNLVATCDL 
      RFPIKLESL SLLHGQFCS YEPELFPGI 
      IYRMIKPHL CLLIFVNGK IVFTGSKSR 
      EGIKESLDN IYPILRSFK KN
    CDS sequence
    • >TC007228 RA
      atggaacaa aaacaaaca gacgtgcac ttgcgtgca 
      atgttgaat agccctgca cgttttact ccagctgtc 
      cctaccccg gactggtcc gcaggccca gctgtgccc 
      ccttcacct cagattccc ggaaaatct aacatgatt 
      gtggcaact ccaggttca gcagctggt gaacctcat 
      acaaacata acaatacaa aattgcgta accacggtt 
      gatctcaat acaaaacta gacctggcg ttaattaac 
      gcgaggacc agaaactcg gaatataac ccagcacgg 
      tttcatggg ttaattatg agacttaga gatccaaga 
      accacagct ttgatattc cagactggt aaaattgta 
      tgcactggg gctaaaagt gagcaagat gcgctcctg 
      gcgtctaaa aagtttgcc cggattata caaaaattg 
      ggctttgat atcaaattt gacaacttt aagatacag 
      aatctggtg gcgacgtgc gatttgaga ttccccata 
      aaattggaa agtttaagt ttattgcat ggacagttt 
      tgtagttac gaacctgaa ctttttcct ggaataatc 
      taccgcatg attaaacca cacttgtgt ttgcttatt 
      tttgtcaat gggaaaatt gttttcaca ggctccaag 
      tcgcgagaa ggaattaag gaatcgctg gataatatt 
      tacccaatt ttacgtagc tttaagaaa aattaa
    • >TC007228 RB
      atggaacaa aaacaaaca gacgtgcac ttgcgtgca 
      atgttgaat agccctgca cgttttact ccagctgtc 
      cctaccccg gactggtcc gcaggccca gctgtgccc 
      ccttcaatt cccggaaaa tctaacatg attgtggca 
      actccaggt tcagcagct ggtgaacct catacaaac 
      ataacaata caaaattgc gtaaccacg gttgatctc 
      aatacaaaa ctagacctg gcgttaatt aacgcgagg 
      accagaaac tcggaatat aacccagca cggtttcat 
      gggttaatt atgagactt agagatcca agaaccaca 
      gctttgata ttccagact ggtaaaatt gtatgcact 
      ggggctaaa agtgagcaa gatgcgctc ctggcgtct 
      aaaaagttt gcccggatt atacaaaaa ttgggcttt 
      gatatcaaa tttgacaac tttaagata cagaatctg 
      gtggcgacg tgcgatttg agattcccc ataaaattg 
      gaaagttta agtttattg catggacag ttttgtagt 
      tacgaacct gaacttttt cctggaata atctaccgc 
      atgattaaa ccacacttg tgtttgctt atttttgtc 
      aatgggaaa attgttttc acaggctcc aagtcgcga 
      gaaggaatt aaggaatcg ctggataat atttaccca 
      attttacgt agctttaag aaaaattaa
    mRNA sequence
    • >TC007228 RA
      aaataatca cagaaaaat ggaacaaaa acaaacaga 
      cgtgcactt gcgtgcaat gttgaatag ccctgcacg 
      ttttactcc agctgtccc taccccgga ctggtccgc 
      aggcccagc tgtgccccc ttcacctca gattcccgg 
      aaaatctaa catgattgt ggcaactcc aggttcagc 
      agctggtga acctcatac aaacataac aatacaaaa 
      ttgcgtaac cacggttga tctcaatac aaaactaga 
      cctggcgtt aattaacgc gaggaccag aaactcgga 
      atataaccc agcacggtt tcatgggtt aattatgag 
      acttagaga tccaagaac cacagcttt gatattcca 
      gactggtaa aattgtatg cactggggc taaaagtga 
      gcaagatgc gctcctggc gtctaaaaa gtttgcccg 
      gattataca aaaattggg ctttgatat caaatttga 
      caactttaa gatacagaa tctggtggc gacgtgcga 
      tttgagatt ccccataaa attggaaag tttaagttt 
      attgcatgg acagttttg tagttacga acctgaact 
      ttttcctgg aataatcta ccgcatgat taaaccaca 
      cttgtgttt gcttatttt tgtcaatgg gaaaattgt 
      tttcacagg ctccaagtc gcgagaagg aattaagga 
      atcgctgga taatattta cccaatttt acgtagctt 
      taagaaaaa ttaagtaga ctggttctt tgagatatt 
      actaaattt ttaataatg gatattggt ccatagagg 
      gcactggta acaatttct ctcttttac gcaaatgac 
      gtaataaaa ttattttta cacgtgtat tcctcaccg 
      taattattt cttgattca agttaagac ttctacgcc 
      ctgttattg tatttatgt caaaggttt tatagattt 
      ttaaaatag ttaatagaa cgatgttat tagttttac 
      cgctatttt acagtgaag tactactat taaaatcga 
      tcacctgag gccgcaaca gtcataatt tgaatcgga 
      aactgagtc aatctttgt gagcaatta ttcaaaaca 
      cgagttttt atttaggtc ggcgttaat agccgtaga 
      catacgtaa ggcttcaaa cagccaatt ttgttttat 
      taagttttc ttcgatttt attttgtta attgaaaat 
      aaattgtac ttttgtaaa tctagcgta tttcaatat 
      cgattgaac tttgttcat tccaaggca ataatccga 
      accaaaagg ttgaaactc ccctttatt attcctctt 
      ttgccttct ccttttctt tgtacaaga tagaaagga 
      taaatttga tcaaatcgt accctttga gatttttct 
      aactcagat aaactgttt tataacaat acatatgtt 
      aggtcgtta atttattta ttattatta gattaggcc 
      acttaggat cacggttta tctgtattg tatttcatt 
      gtttgtata ttttttgtt agcaataaa ctgcgtttg 
      atatt
    • >TC007228 RB
      aaataatca cagaaaaat ggaacaaaa acaaacaga 
      cgtgcactt gcgtgcaat gttgaatag ccctgcacg 
      ttttactcc agctgtccc taccccgga ctggtccgc 
      aggcccagc tgtgccccc ttcaattcc cggaaaatc 
      taacatgat tgtggcaac tccaggttc agcagctgg 
      tgaacctca tacaaacat aacaataca aaattgcgt 
      aaccacggt tgatctcaa tacaaaact agacctggc 
      gttaattaa cgcgaggac cagaaactc ggaatataa 
      cccagcacg gtttcatgg gttaattat gagacttag 
      agatccaag aaccacagc tttgatatt ccagactgg 
      taaaattgt atgcactgg ggctaaaag tgagcaaga 
      tgcgctcct ggcgtctaa aaagtttgc ccggattat 
      acaaaaatt gggctttga tatcaaatt tgacaactt 
      taagataca gaatctggt ggcgacgtg cgatttgag 
      attccccat aaaattgga aagtttaag tttattgca 
      tggacagtt ttgtagtta cgaacctga actttttcc 
      tggaataat ctaccgcat gattaaacc acacttgtg 
      tttgcttat ttttgtcaa tgggaaaat tgttttcac 
      aggctccaa gtcgcgaga aggaattaa ggaatcgct 
      ggataatat ttacccaat tttacgtag ctttaagaa 
      aaattaagt agactggtt ctttgagat attactaaa 
      tttttaata atggatatt ggtccatag agggcactg 
      gtaacaatt tctctcttt tacgcaaat gacgtaata 
      aaattattt ttacacgtg tattcctca ccgtaatta 
      tttcttgat tcaagttaa gacttctac gccctgtta 
      ttgtattta tgtcaaagg ttttataga tttttaaaa 
      tagttaata gaacgatgt tattagttt taccgctat 
      tttacagtg aagtactac tattaaaat cgatcacct 
      gaggccgca acagtcata atttgaatc ggaaactga 
      gtcaatctt tgtgagcaa ttattcaaa acacgagtt 
      tttatttag gtcggcgtt aatagccgt agacatacg 
      taaggcttc aaacagcca attttgttt tattaagtt 
      ttcttcgat tttattttg ttaattgaa aataaattg 
      tacttttgt aaatctagc gtatttcaa tatcgattg 
      aactttgtt cattccaag gcaataatc cgaaccaaa 
      aggttgaaa ctccccttt attattcct cttttgcct 
      tctcctttt ctttgtaca agatagaaa ggataaatt 
      tgatcaaat cgtaccctt tgagatttt tctaactca 
      gataaactg ttttataac aatacatat gttaggtcg 
      ttaatttat ttattatta ttagattag gccacttag 
      gatcacggt ttatctgta ttgtatttc attgtttgt 
      atatttttt gttagcaat aaactgcgt ttgatatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY