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Gene information
General information
TC007221 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7383875..7384882
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007221
    iBeetle screen iB_01150

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007221 RA
      MSKRKFNEG DFSQNKRRQ VKFNAKKHS LDSDEEDDY 
      DETAEELNA DDIEGEEEG ISRQVEGVR MTAFNMKEE 
      MEEGHFDKE GHFIWNNEK EIRDNWLDN IDWHKVNSD 
      DKYQLDPNE KGLGAESDS ESEAEDFDE VGLYKKMLN 
      YMKPKETVN KCLRRLGGD LKLSSVERL KRKKAGTLN 
      SNQDVADMT EIANSILTK TGNMNVYQE TFEEITKKI 
      DDRTKPKNS EPVLDMYAD DFGDKEKEK METASVSGK 
      PEQLMWEFK WDQDKDQVE GPYDTSQML KWSKEGYFK 
      TGVWVRKCG EQSNFYTSN RIDFELYD
    CDS sequence
    • >TC007221 RA
      atgtcaaaa cgaaaattc aacgaaggc gatttttcg 
      caaaataag cggcgccag gtgaagttt aacgcaaaa 
      aaacattcc ttggattcg gacgaagag gacgattac 
      gatgagacc gctgaagag ctcaacgcc gacgacatc 
      gagggcgaa gaggaggga atttcgcgt caggttgaa 
      ggggtccgg atgaccgcc ttcaacatg aaagaggag 
      atggaagaa gggcatttc gacaaagag ggccacttt 
      atttggaac aacgagaag gaaatacgg gataattgg 
      ttggacaac atcgactgg cataaagtc aacagtgac 
      gataagtac cagttggac cccaacgag aaaggcttg 
      ggggccgag agtgattct gagtcggaa gctgaggat 
      ttcgatgag gtcggtttg tacaaaaag atgctcaac 
      tacatgaaa cccaaagaa acggtcaac aagtgtttg 
      aggcggtta gggggcgat ttgaagttg tccagtgtc 
      gagagactg aagcgcaag aaggcaggg actttgaat 
      tcgaatcag gatgtggcg gacatgacc gagatcgcg 
      aatagtata ttaacgaag acggggaac atgaacgta 
      taccaggag acttttgaa gaaattacc aaaaaaatt 
      gacgaccga acgaaaccg aaaaacagt gagcctgtt 
      ctggacatg tatgctgac gacttcggc gacaaggag 
      aaggagaag atggagacg gcgtcagtg tcaggtaaa 
      ccagagcag ttaatgtgg gaatttaag tgggaccaa 
      gacaaggac caagtcgag ggtccgtac gacacctca 
      caaatgtta aagtggtcc aaagaaggg tacttcaag 
      acgggagtg tgggtgcgt aagtgtggc gaacaatcc 
      aacttttac acctcgaac cggatagac ttcgagctg 
      tatgactaa
    mRNA sequence
    • >TC007221 RA
      cgtgttgtt tgttttgca tttgtcaaa aatgtcaaa 
      acgaaaatt caacgaagg cgatttttc gcaaaataa 
      gcggcgcca ggtgaagtt taacgcaaa aaaacattc 
      cttggattc ggacgaaga ggacgatta cgatgagac 
      cgctgaaga gctcaacgc cgacgacat cgagggcga 
      agaggaggg aatttcgcg tcaggttga aggggtccg 
      gatgaccgc cttcaacat gaaagagga gatggaaga 
      agggcattt cgacaaaga gggccactt tatttggaa 
      caacgagaa ggaaatacg ggataattg gttggacaa 
      catcgactg gcataaagt caacagtga cgataagta 
      ccagttgga ccccaacga gaaaggctt gggggccga 
      gagtgattc tgagtcgga agctgagga tttcgatga 
      ggtcggttt gtacaaaaa gatgctcaa ctacatgaa 
      acccaaaga aacggtcaa caagtgttt gaggcggtt 
      agggggcga tttgaagtt gtccagtgt cgagagact 
      gaagcgcaa gaaggcagg gactttgaa ttcgaatca 
      ggatgtggc ggacatgac cgagatcgc gaatagtat 
      attaacgaa gacggggaa catgaacgt ataccagga 
      gacttttga agaaattac caaaaaaat tgacgaccg 
      aacgaaacc gaaaaacag tgagcctgt tctggacat 
      gtatgctga cgacttcgg cgacaagga gaaggagaa 
      gatggagac ggcgtcagt gtcaggtaa accagagca 
      gttaatgtg ggaatttaa gtgggacca agacaagga 
      ccaagtcga gggtccgta cgacacctc acaaatgtt 
      aaagtggtc caaagaagg gtacttcaa gacgggagt 
      gtgggtgcg taagtgtgg cgaacaatc caactttta 
      cacctcgaa ccggataga cttcgagct gtatgacta 
      acagtgtta taaaaattt tattatcaa tatacataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY