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Gene information
General information
TC007213 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 7480315..7482032
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007213
    iBeetle screen iB_01147
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 87.5% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007213 RA
      MSDSAGPSS SSDQRQRGL NVEALKQHV LTHKIDCGL 
      WAIRVLTIL FTCGYFLPI FGSAQNAYY KALIANAAI 
      NALRLHQRL GRVRLSREF VAQLLMEDS CHYLFYSMI 
      FIYVAPLTL VLLPVVLFA ILHSASYSL TLLDTLGQN 
      SWWGARLLI SLVEFQSLN ILRLIAFTE IIMMPLTVL 
      FVAMGRASL LTPFIYYHF LTQRYTSRR NPYNRNMFH 
      ELRILFEKT AAKPNLPSF VKSILLGII NFTVKLAPP 
      QVQAQHQQ
    CDS sequence
    • >TC007213 RA
      atgagtgac agtgcgggc ccatcgagc agttcggac 
      caaagacag cgggggctg aacgtggag gccctcaag 
      cagcacgtg ctcacccac aaaatcgac tgtgggttg 
      tgggccatt cgggtgctg accatcctc ttcacttgc 
      ggctacttt ttgccaatt ttcgggagt gctcaaaat 
      gcctattac aaggcgctc attgcgaac gctgccatt 
      aacgccctc aggctgcac cagaggttg gggagggtt 
      aggctgagt cgggagttt gtggcacag ttgttgatg 
      gaggatagc tgccactat ctcttctat tccatgatt 
      tttatctat gtggcaccc ttgaccttg gttttgttg 
      ccagtggtt ctgttcgct attctacat tcagccagt 
      tactcacta actctactt gatactttg ggccaaaac 
      tcgtggtgg ggcgcccgc ctcttgata tctttggtc 
      gaattccag tcactaaat atcttgaga ctgatcgca 
      ttcactgag attattatg atgccactc actgtactt 
      tttgtcgct atgggacgg gctagtcta ctgacgcct 
      tttatctat tatcatttt ttgactcaa aggtacaca 
      tcaaggcgt aacccttac aatagaaac atgttccat 
      gaactcaga atcttgttc gaaaagacc gcagcaaag 
      cccaatcta ccttccttt gtgaagtca atcttgcta 
      gggattatc aatttcact gttaagtta gcacctccc 
      caggtgcaa gcccagcat cagcagtga
    mRNA sequence
    • >TC007213 RA
      caaacgtca aaatcggac gcaatctca tggcaacgt 
      aaatgtcac tatgtcatt gcgtgttta cgagcaagt 
      gagatatgc acgtatcgt cctgtttgt aataaaaat 
      caaacatta acattattc ggtcgtgct atttcgcga 
      atttgctaa ccacctacg tgtcactgt aaaccgttg 
      gtgtttttc caaatctcg aagaaatga gtgacagtg 
      cgggcccat cgagcagtt cggaccaaa gacagcggg 
      ggctgaacg tggaggccc tcaagcagc acgtgctca 
      cccacaaaa tcgactgtg ggttgtggg ccattcggg 
      tgctgacca tcctcttca cttgcggct actttttgc 
      caattttcg ggagtgctc aaaatgcct attacaagg 
      cgctcattg cgaacgctg ccattaacg ccctcaggc 
      tgcaccaga ggttgggga gggttaggc tgagtcggg 
      agtttgtgg cacagttgt tgatggagg atagctgcc 
      actatctct tctattcca tgattttta tctatgtgg 
      cacccttga ccttggttt tgttgccag tggttctgt 
      tcgctattc tacattcag ccagttact cactaactc 
      tacttgata ctttgggcc aaaactcgt ggtggggcg 
      cccgcctct tgatatctt tggtcgaat tccagtcac 
      taaatatct tgagactga tcgcattca ctgagatta 
      ttatgatgc cactcactg tactttttg tcgctatgg 
      gacgggcta gtctactga cgcctttta tctattatc 
      attttttga ctcaaaggt acacatcaa ggcgtaacc 
      cttacaata gaaacatgt tccatgaac tcagaatct 
      tgttcgaaa agaccgcag caaagccca atctacctt 
      cctttgtga agtcaatct tgctaggga ttatcaatt 
      tcactgtta agttagcac ctccccagg tgcaagccc 
      agcatcagc agtgagcta gttgttcca tgtatcctt 
      attatatca aatgttcgt gatatgggt aattttaac 
      gcctagtat tgtttattt gcacttgtt tggtacatg 
      ttcatgcaa ttttttgca cgaatttga ggtatggtt 
      gcaaaattt cgtccgtta ctttttata tacttttaa 
      cccaggaat gatttttta ttaattttt agtatggat 
      ggtgtaaag tagttttgt tatatcgtt ataaatgca 
      ttgtgctca tggaggttt ctgttgtaa aactcattc 
      gaattaaat tttctccat gtcatgttg ttatgttaa 
      taagcaaaa gtttgtaat ttcacaata aaaaaatat 
      ttaaaaa