Status
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Gene information
General information
TC007210 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7502165..7504262
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007210
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01146

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 3

    tropharium partially degenerated - Number of animals showing the phenotype: 3

    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature potentially degenerated - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007210 RA
      METSDSEYF ESADEDFQS DDEDTKTVS SVTKTEEKP 
NTKCVNDAI KKLEHVQLE DTQEKNSLN NNKKNDKGK 
TNKEIEDNI NSNEITEDL AQIQATKGP GSKLNKVEA 
SHPTSVKKR SKETDVPKK LGAKLSCKD SVAKPPQES 
PECVKNVDQ KQPKESPEC VKSADQKQP KSETSNIEE 
NLWDESEID WGKENEDDL WGNDDDWEP VNEPTVPPQ 
REKIEPAQV KVPSSSSWG SWGTWDVSS IISTATQSV 
STLTQGIST VLETGIGVP DPEELAKIN QAEKEKLKE 
IVPDEDTDN SGFGLGNLV MGVSQLTKL VETTGTKVI 
SGGLDTLET IGKKTMEVL QEGDPGLKK KRAFLKIDQ 
DKPVLSQLL REAKEKAEE ENKILEEKH FAKKAKNYE 
TLFDDHQGL VHLEALEML SKQCDIKLQ TLLESYTDE 
ALTEMQETM DQVKELCEL PDEEEDEHA NWTEIKEKL 
ESAVSEISI PISYKKFVE TWEETEAWL NNLKLEICD 
ARELHQQAV ETLAQLTAI AVEQFHKAG ELLLVKEHR 
STADEADSL VQLTTTLTS LIGVVAAKF SDKLNTKSS 
SSKNKEEIN SLITNVFFE AANSSSYIQ DAFQLLIPV 
LQVGAV
    CDS sequence
    • >TC007210 RA
      atggagacg tcagacagt gaatatttt gagagtgca 
gacgaagat tttcaatca gacgatgaa gacaccaaa 
actgtgtca agtgttaca aaaactgag gaaaaacct 
aacacaaag tgtgttaat gacgcaata aaaaagtta 
gaacacgta caattagag gatactcaa gaaaaaaat 
agtttgaac aacaataaa aagaacgac aagggtaaa 
actaacaaa gaaatagaa gacaacatt aattccaat 
gagattact gaagatctt gcacaaatt caggcaaca 
aaaggtccc ggaagtaaa ctcaataaa gttgaagcc 
tcacatcca acaagtgtt aaaaagcgc agtaaagaa 
acagacgtt ccgaaaaaa ctaggtgct aagttgagt 
tgtaaagac agtgttgca aaaccaccc caagaaagt 
ccagagtgt gttaaaaat gttgatcaa aaacaacca 
aaagaaagt ccagagtgc gttaaaagt gctgatcaa 
aaacaacca aagagtgaa acttccaac atagaagaa 
aacctctgg gatgaatct gaaatagac tggggaaag 
gaaaatgaa gacgacttg tggggtaac gatgatgat 
tgggaaccc gtgaatgaa ccaacggtg cccccccag 
cgtgaaaaa attgaaccc gctcaagta aaagtgcct 
tcttccagc agttggggc tcatggggc acctgggac 
gtctcctca atcatatcg acagcaact caaagcgtc 
tcaacgcta acgcaagga attagcacg gtccttgaa 
acgggaatt ggagtcccg gacccggaa gaactagcc 
aaaataaac caagctgag aaagagaaa ttgaaagaa 
atcgttcct gacgaggac acggacaat tctggtttt 
gggctgggt aacttagtg atgggggtg tcacaactc 
actaaatta gttgaaaca actggtacc aaagtcatt 
tccggtggt ttggacacg ttggagaca atcgggaag 
aaaacaatg gaagtactg caagagggt gacccaggt 
ttgaaaaag aaacgagcc ttcttgaag atagatcaa 
gacaaaccg gtcctttcc caattacta cgcgaagcg 
aaggagaaa gctgaggaa gaaaacaaa attctggaa 
gagaaacat ttcgcaaaa aaggcgaaa aattacgaa 
actttgttt gacgaccat cagggtctt gtacacctg 
gaggcgtta gagatgctt tcgaaacag tgtgatatt 
aagttacag acgttgttg gaatcttac actgatgaa 
gctttgact gaaatgcag gagacgatg gatcaggtg 
aaggagctt tgcgagttg cctgatgaa gaagaggat 
gaacatgca aactggacc gagatcaaa gaaaaatta 
gagtcagct gttagtgaa atcagtatc cctatttcg 
tacaagaaa tttgttgag acttgggag gaaactgag 
gcgtggttg aataacttg aaactcgaa atttgcgac 
gccagagag ttacaccaa caagctgtt gaaactttg 
gcacagttg actgcaata gcagtggaa cagtttcac 
aaagctggg gaactgtta ctggtgaag gaacatagg 
agtacggct gatgaggcg gatagttta gtccagttg 
acaacaact ttaacatcg ctaattggc gtcgttgca 
gcaaaattc tcagacaaa ctgaacacc aaaagttcc 
agctcgaaa aacaaagaa gaaataaac agtctcata 
acgaatgta ttctttgag gccgcaaat agtagttca 
tacattcag gacgctttt cagctttta atacctgtt 
cttcaagtg ggcgcggtg taa
    mRNA sequence
    • >TC007210 RA
      agctgttga caactcact catgttttg tgacaaaca 
gctgtttta ttaaacagt ttcgaaatt gagttgttt 
tttggacgt aaaataaga aatggagac gtcagacag 
tgaatattt tgagagtgc agacgaaga ttttcaatc 
agacgatga agacaccaa aactgtgtc aagtgttac 
aaaaactga ggaaaaacc taacacaaa gtgtgttaa 
tgacgcaat aaaaaagtt agaacacgt acaattaga 
ggatactca agaaaaaaa tagtttgaa caacaataa 
aaagaacga caagggtaa aactaacaa agaaataga 
agacaacat taattccaa tgagattac tgaagatct 
tgcacaaat tcaggcaac aaaaggtcc cggaagtaa 
actcaataa agttgaagc ctcacatcc aacaagtgt 
taaaaagcg cagtaaaga aacagacgt tccgaaaaa 
actaggtgc taagttgag ttgtaaaga cagtgttgc 
aaaaccacc ccaagaaag tccagagtg tgttaaaaa 
tgttgatca aaaacaacc aaaagaaag tccagagtg 
cgttaaaag tgctgatca aaaacaacc aaagagtga 
aacttccaa catagaaga aaacctctg ggatgaatc 
tgaaataga ctggggaaa ggaaaatga agacgactt 
gtggggtaa cgatgatga ttgggaacc cgtgaatga 
accaacggt gccccccca gcgtgaaaa aattgaacc 
cgctcaagt aaaagtgcc ttcttccag cagttgggg 
ctcatgggg cacctggga cgtctcctc aatcatatc 
gacagcaac tcaaagcgt ctcaacgct aacgcaagg 
aattagcac ggtccttga aacgggaat tggagtccc 
ggacccgga agaactagc caaaataaa ccaagctga 
gaaagagaa attgaaaga aatcgttcc tgacgagga 
cacggacaa ttctggttt tgggctggg taacttagt 
gatgggggt gtcacaact cactaaatt agttgaaac 
aactggtac caaagtcat ttccggtgg tttggacac 
gttggagac aatcgggaa gaaaacaat ggaagtact 
gcaagaggg tgacccagg tttgaaaaa gaaacgagc 
cttcttgaa gatagatca agacaaacc ggtcctttc 
ccaattact acgcgaagc gaaggagaa agctgagga 
agaaaacaa aattctgga agagaaaca tttcgcaaa 
aaaggcgaa aaattacga aactttgtt tgacgacca 
tcagggtct tgtacacct ggaggcgtt agagatgct 
ttcgaaaca gtgtgatat taagttaca gacgttgtt 
ggaatctta cactgatga agctttgac tgaaatgca 
ggagacgat ggatcaggt gaaggagct ttgcgagtt 
gcctgatga agaagagga tgaacatgc aaactggac 
cgagatcaa agaaaaatt agagtcagc tgttagtga 
aatcagtat ccctatttc gtacaagaa atttgttga 
gacttggga ggaaactga ggcgtggtt gaataactt 
gaaactcga aatttgcga cgccagaga gttacacca 
acaagctgt tgaaacttt ggcacagtt gactgcaat 
agcagtgga acagtttca caaagctgg ggaactgtt 
actggtgaa ggaacatag gagtacggc tgatgaggc 
ggatagttt agtccagtt gacaacaac tttaacatc 
gctaattgg cgtcgttgc agcaaaatt ctcagacaa 
actgaacac caaaagttc cagctcgaa aaacaaaga 
agaaataaa cagtctcat aacgaatgt attctttga 
ggccgcaaa tagtagttc atacattca ggacgcttt 
tcagctttt aatacctgt tcttcaagt gggcgcggt 
gtaatcata agtctcaac attatgtaa ataacaagt 
tgctaattt at

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY