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Gene information
General information
TC007210 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7502165..7504262
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007210
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01146

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 3

    tropharium partially degenerated - Number of animals showing the phenotype: 3

    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature potentially degenerated - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007210 RA
      METSDSEYF ESADEDFQS DDEDTKTVS SVTKTEEKP 
      NTKCVNDAI KKLEHVQLE DTQEKNSLN NNKKNDKGK 
      TNKEIEDNI NSNEITEDL AQIQATKGP GSKLNKVEA 
      SHPTSVKKR SKETDVPKK LGAKLSCKD SVAKPPQES 
      PECVKNVDQ KQPKESPEC VKSADQKQP KSETSNIEE 
      NLWDESEID WGKENEDDL WGNDDDWEP VNEPTVPPQ 
      REKIEPAQV KVPSSSSWG SWGTWDVSS IISTATQSV 
      STLTQGIST VLETGIGVP DPEELAKIN QAEKEKLKE 
      IVPDEDTDN SGFGLGNLV MGVSQLTKL VETTGTKVI 
      SGGLDTLET IGKKTMEVL QEGDPGLKK KRAFLKIDQ 
      DKPVLSQLL REAKEKAEE ENKILEEKH FAKKAKNYE 
      TLFDDHQGL VHLEALEML SKQCDIKLQ TLLESYTDE 
      ALTEMQETM DQVKELCEL PDEEEDEHA NWTEIKEKL 
      ESAVSEISI PISYKKFVE TWEETEAWL NNLKLEICD 
      ARELHQQAV ETLAQLTAI AVEQFHKAG ELLLVKEHR 
      STADEADSL VQLTTTLTS LIGVVAAKF SDKLNTKSS 
      SSKNKEEIN SLITNVFFE AANSSSYIQ DAFQLLIPV 
      LQVGAV
    CDS sequence
    • >TC007210 RA
      atggagacg tcagacagt gaatatttt gagagtgca 
      gacgaagat tttcaatca gacgatgaa gacaccaaa 
      actgtgtca agtgttaca aaaactgag gaaaaacct 
      aacacaaag tgtgttaat gacgcaata aaaaagtta 
      gaacacgta caattagag gatactcaa gaaaaaaat 
      agtttgaac aacaataaa aagaacgac aagggtaaa 
      actaacaaa gaaatagaa gacaacatt aattccaat 
      gagattact gaagatctt gcacaaatt caggcaaca 
      aaaggtccc ggaagtaaa ctcaataaa gttgaagcc 
      tcacatcca acaagtgtt aaaaagcgc agtaaagaa 
      acagacgtt ccgaaaaaa ctaggtgct aagttgagt 
      tgtaaagac agtgttgca aaaccaccc caagaaagt 
      ccagagtgt gttaaaaat gttgatcaa aaacaacca 
      aaagaaagt ccagagtgc gttaaaagt gctgatcaa 
      aaacaacca aagagtgaa acttccaac atagaagaa 
      aacctctgg gatgaatct gaaatagac tggggaaag 
      gaaaatgaa gacgacttg tggggtaac gatgatgat 
      tgggaaccc gtgaatgaa ccaacggtg cccccccag 
      cgtgaaaaa attgaaccc gctcaagta aaagtgcct 
      tcttccagc agttggggc tcatggggc acctgggac 
      gtctcctca atcatatcg acagcaact caaagcgtc 
      tcaacgcta acgcaagga attagcacg gtccttgaa 
      acgggaatt ggagtcccg gacccggaa gaactagcc 
      aaaataaac caagctgag aaagagaaa ttgaaagaa 
      atcgttcct gacgaggac acggacaat tctggtttt 
      gggctgggt aacttagtg atgggggtg tcacaactc 
      actaaatta gttgaaaca actggtacc aaagtcatt 
      tccggtggt ttggacacg ttggagaca atcgggaag 
      aaaacaatg gaagtactg caagagggt gacccaggt 
      ttgaaaaag aaacgagcc ttcttgaag atagatcaa 
      gacaaaccg gtcctttcc caattacta cgcgaagcg 
      aaggagaaa gctgaggaa gaaaacaaa attctggaa 
      gagaaacat ttcgcaaaa aaggcgaaa aattacgaa 
      actttgttt gacgaccat cagggtctt gtacacctg 
      gaggcgtta gagatgctt tcgaaacag tgtgatatt 
      aagttacag acgttgttg gaatcttac actgatgaa 
      gctttgact gaaatgcag gagacgatg gatcaggtg 
      aaggagctt tgcgagttg cctgatgaa gaagaggat 
      gaacatgca aactggacc gagatcaaa gaaaaatta 
      gagtcagct gttagtgaa atcagtatc cctatttcg 
      tacaagaaa tttgttgag acttgggag gaaactgag 
      gcgtggttg aataacttg aaactcgaa atttgcgac 
      gccagagag ttacaccaa caagctgtt gaaactttg 
      gcacagttg actgcaata gcagtggaa cagtttcac 
      aaagctggg gaactgtta ctggtgaag gaacatagg 
      agtacggct gatgaggcg gatagttta gtccagttg 
      acaacaact ttaacatcg ctaattggc gtcgttgca 
      gcaaaattc tcagacaaa ctgaacacc aaaagttcc 
      agctcgaaa aacaaagaa gaaataaac agtctcata 
      acgaatgta ttctttgag gccgcaaat agtagttca 
      tacattcag gacgctttt cagctttta atacctgtt 
      cttcaagtg ggcgcggtg taa
    mRNA sequence
    • >TC007210 RA
      agctgttga caactcact catgttttg tgacaaaca 
      gctgtttta ttaaacagt ttcgaaatt gagttgttt 
      tttggacgt aaaataaga aatggagac gtcagacag 
      tgaatattt tgagagtgc agacgaaga ttttcaatc 
      agacgatga agacaccaa aactgtgtc aagtgttac 
      aaaaactga ggaaaaacc taacacaaa gtgtgttaa 
      tgacgcaat aaaaaagtt agaacacgt acaattaga 
      ggatactca agaaaaaaa tagtttgaa caacaataa 
      aaagaacga caagggtaa aactaacaa agaaataga 
      agacaacat taattccaa tgagattac tgaagatct 
      tgcacaaat tcaggcaac aaaaggtcc cggaagtaa 
      actcaataa agttgaagc ctcacatcc aacaagtgt 
      taaaaagcg cagtaaaga aacagacgt tccgaaaaa 
      actaggtgc taagttgag ttgtaaaga cagtgttgc 
      aaaaccacc ccaagaaag tccagagtg tgttaaaaa 
      tgttgatca aaaacaacc aaaagaaag tccagagtg 
      cgttaaaag tgctgatca aaaacaacc aaagagtga 
      aacttccaa catagaaga aaacctctg ggatgaatc 
      tgaaataga ctggggaaa ggaaaatga agacgactt 
      gtggggtaa cgatgatga ttgggaacc cgtgaatga 
      accaacggt gccccccca gcgtgaaaa aattgaacc 
      cgctcaagt aaaagtgcc ttcttccag cagttgggg 
      ctcatgggg cacctggga cgtctcctc aatcatatc 
      gacagcaac tcaaagcgt ctcaacgct aacgcaagg 
      aattagcac ggtccttga aacgggaat tggagtccc 
      ggacccgga agaactagc caaaataaa ccaagctga 
      gaaagagaa attgaaaga aatcgttcc tgacgagga 
      cacggacaa ttctggttt tgggctggg taacttagt 
      gatgggggt gtcacaact cactaaatt agttgaaac 
      aactggtac caaagtcat ttccggtgg tttggacac 
      gttggagac aatcgggaa gaaaacaat ggaagtact 
      gcaagaggg tgacccagg tttgaaaaa gaaacgagc 
      cttcttgaa gatagatca agacaaacc ggtcctttc 
      ccaattact acgcgaagc gaaggagaa agctgagga 
      agaaaacaa aattctgga agagaaaca tttcgcaaa 
      aaaggcgaa aaattacga aactttgtt tgacgacca 
      tcagggtct tgtacacct ggaggcgtt agagatgct 
      ttcgaaaca gtgtgatat taagttaca gacgttgtt 
      ggaatctta cactgatga agctttgac tgaaatgca 
      ggagacgat ggatcaggt gaaggagct ttgcgagtt 
      gcctgatga agaagagga tgaacatgc aaactggac 
      cgagatcaa agaaaaatt agagtcagc tgttagtga 
      aatcagtat ccctatttc gtacaagaa atttgttga 
      gacttggga ggaaactga ggcgtggtt gaataactt 
      gaaactcga aatttgcga cgccagaga gttacacca 
      acaagctgt tgaaacttt ggcacagtt gactgcaat 
      agcagtgga acagtttca caaagctgg ggaactgtt 
      actggtgaa ggaacatag gagtacggc tgatgaggc 
      ggatagttt agtccagtt gacaacaac tttaacatc 
      gctaattgg cgtcgttgc agcaaaatt ctcagacaa 
      actgaacac caaaagttc cagctcgaa aaacaaaga 
      agaaataaa cagtctcat aacgaatgt attctttga 
      ggccgcaaa tagtagttc atacattca ggacgcttt 
      tcagctttt aatacctgt tcttcaagt gggcgcggt 
      gtaatcata agtctcaac attatgtaa ataacaagt 
      tgctaattt at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY