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Gene information
General information
TC007205 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7555883..7558471
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007205
    iBeetle screen iB_01144

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 1

    vitellogenic egg chamber potentially lateshape broader - Number of animals showing the phenotype: 2

    lateral oviduct potentially latedegenerated - Number of animals showing the phenotype: 2

    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007205 RA
      MFNLIKNIV YIHAKSPKS KICRVKRAV ITRTYCEAP 
      IDYTQIPIE RIRNFSIIA HVDHGKSTL ADRLLEFTG 
      AITKNSGQN QVLDKLQVE RERGITVKA QSVSLLYNY 
      KGNEYLLNL IDTPGHVDF SSEVSRSLS ACQGVILVV 
      DANDGVQAQ TVANFYLAF GKDLTIVPV LNKIDLKNA 
      DPDRVTKQL QSLFDIEPQ EVLRISAKL GTGIQSVIE 
      ALIERISPP KVDRTAPFR ALLFDSWFD KYRGALSLI 
      YIQDGEINV GDEVQSCHS QKTYSIKSL SLLRPQEVV 
      TKKLVAGQI GLIGCNMRT SKEAFIGDT IHLSSCPVE 
      PLQGFEPPQ PMVFAGVYP MDQSQHVNL RSAIDKLTL 
      NDSAVSVDI DSSPALGQG WRLGFLGLL HLEVFSQRL 
      QQEYGAEPI LTAPSVTYK ITLKETKQN IKTGREIFV 
      NNPALWPDT VHIEETFEP MVIGTIITP DKYLGPIMS 
      LCMERRGVQ RNATNIDND RVMLQYDLP LCEIIIDFH 
      DSLKTISSG YASFDYEDN GYQSSNLVK LRILLNGTA 
      VEELSNIVH ISKAQSIGR KMVLKLKEM IPRQMIQIA 
      VQAVCNGKI LARETIKAY RKDVTAKLY GGDVTRRMK 
      LLAQQAAGK KKMRMVANI SLPRETFID VLKK
    • >TC007205 RB
      MFNLIKNIV YIHAKSPKS KICRVKWRA VITRTYCEA 
      PIDYTQIPI ERIRNFSII AHVDHGKST LADRLLEFT 
      GAITKNSGQ NQVLDKLQV ERERGITVK AQSVSLLYN 
      YKGNEYLLN LIDTPGHVD FSSEVSRSL SACQGVILV 
      VDANDGVQA QTVANFYLA FGKDLTIVP VLNKIDLKN 
      ADPDRVTKQ LQSLFDIEP QEVLRISAK LGTGIQSVI 
      EALIERISP PKVDRTAPF RALLFDSWF DKYRGALSL 
      IYIQDGEIN VGDEVQSCH SQKTYSIKS LSLLRPQEV 
      VTKKLVAGQ IGLIGCNMR TSKEAFIGD TIHLSSCPV 
      EPLQGFEPP QPMVFAGVY PMDQSQHVN LRSAIDKLT 
      LNDSAVSVD IDSSPALGQ GWRLGFLGL LHLEVFSQR 
      LQQEYGAEP ILTAPSVTY KITLKETKQ NIKTGREIF 
      VNNPALWPD TVHIEETFE PMVIGTIIT PDKYLGPIM 
      SLCMERRGV QRNATNIDN DRVMLQYDL PLCEIIIDF 
      HDSLKTISS GYASFDYED NGYQSSNLV KLRILLNGT 
      AVEELSNIV HISKAQSIG RKMVLKLKE MIPRQMIQI 
      AVQAVCNGK ILARETIKA YRKDVTAKL YGGDVTRRM 
      KLLAQQAAG KKKMRMVAN ISLPRETFI DVLKK
    CDS sequence
    • >TC007205 RA
      atgtttaat ttaattaag aacatagtt tacatacac 
      gcgaagtca ccaaaatcg aaaatatgt cgtgttaag 
      cgtgctgtt attacaaga acgtattgt gaagccccc 
      atcgattat actcaaatt ccaatcgaa cgaataaga 
      aattttagt ataatagcc cacgttgac catggtaag 
      agtactctg gccgaccgt ctgcttgaa ttcacaggt 
      gccataacg aaaaacagt ggtcaaaac caagttttg 
      gataaatta caagtggag cgcgaacga ggaatcaca 
      gtcaaagcc caaagtgtg tctttatta tataattac 
      aaagggaac gaatattta ttgaattta attgatact 
      ccgggtcat gtcgatttt tccagcgag gtctctaga 
      tcactttca gcctgccaa ggggttatc ctagtggtg 
      gacgccaat gacggggtc caggcccaa actgtggca 
      aatttttac ctagctttt ggcaaagac ctcaccatt 
      gtgcctgtt ttaaataaa attgatttg aaaaatgct 
      gaccccgat agggttacc aaacagtta cagtcactt 
      tttgatata gagcctcag gaagtgcta aggatttct 
      gcaaaatta ggcaccggt attcaatcg gttattgag 
      gctttaatc gagcgaatt tcgcccccg aaagtggac 
      agaacggct ccgtttcga gccctactt ttcgacagt 
      tggttcgat aaatacagg ggggccttg tcgttgatt 
      tatattcaa gatggtgaa ataaatgtg ggggacgaa 
      gtacagtct tgccactcg caaaaaaca tacagcatc 
      aagagttta tctctgtta cggccacaa gaggtcgtt 
      acgaaaaaa ctagtcgcg ggtcagatt ggcttaatc 
      ggttgtaac atgaggacg agtaaagag gcgtttatt 
      ggtgacacc attcacttg tcttcctgc cctgttgag 
      cctcttcaa ggtttcgag ccgccgcag ccaatggtg 
      ttcgccggt gtttaccca atggaccaa tcacagcac 
      gtcaatttg aggagcgct atagataaa ttgactttg 
      aatgattcg gcggtttcg gttgatatt gattcgagt 
      ccggccctg ggtcaaggc tggaggctg ggttttttg 
      gggctcttg catttggag gttttttcg cagcgtttg 
      caacaggaa tacggcgct gaaccgatt ctcacagca 
      ccctctgtc acttataaa attacgtta aaagaaacc 
      aaacaaaat attaagacg gggcgcgag atatttgtt 
      aataatcca gctttgtgg cccgatact gtccacatt 
      gaggagact tttgagccg atggtgatc ggaactatt 
      atcacgcct gataagtat ctggggccg attatgtct 
      ctttgcatg gaaagacga ggtgttcaa agaaatgcc 
      actaacata gacaacgat cgtgttatg ttacaatac 
      gatttgccg ctttgcgaa ataattata gactttcat 
      gacagtcta aaaactatt tcttccggt tatgccagt 
      tttgattat gaagacaac ggataccaa agctctaat 
      cttgttaaa ttgcggatt ttgttgaat gggacagca 
      gttgaagag ttaagcaat attgttcat atctctaaa 
      gctcaatct attggtcgg aaaatggtt ctgaaactg 
      aaagaaatg atccctagg caaatgata caaattgct 
      gtccaggct gtttgcaat ggcaaaatt ttagctagg 
      gagactatc aaagcgtac cgtaaagac gtaacagct 
      aaactttat ggaggtgat gtaacaagg agaatgaaa 
      ctactagcg caacaagcc gccgggaaa aagaaaatg 
      cggatggtt gcaaacatt tccttgccc agagagaca 
      tttatagac gttttgaag aaataa
    • >TC007205 RB
      atgtttaat ttaattaag aacatagtt tacatacac 
      gcgaagtca ccaaaatcg aaaatatgt cgtgttaag 
      tggcgtgct gttattaca agaacgtat tgtgaagcc 
      cccatcgat tatactcaa attccaatc gaacgaata 
      agaaatttt agtataata gcccacgtt gaccatggt 
      aagagtact ctggccgac cgtctgctt gaattcaca 
      ggtgccata acgaaaaac agtggtcaa aaccaagtt 
      ttggataaa ttacaagtg gagcgcgaa cgaggaatc 
      acagtcaaa gcccaaagt gtgtcttta ttatataat 
      tacaaaggg aacgaatat ttattgaat ttaattgat 
      actccgggt catgtcgat ttttccagc gaggtctct 
      agatcactt tcagcctgc caaggggtt atcctagtg 
      gtggacgcc aatgacggg gtccaggcc caaactgtg 
      gcaaatttt tacctagct tttggcaaa gacctcacc 
      attgtgcct gttttaaat aaaattgat ttgaaaaat 
      gctgacccc gatagggtt accaaacag ttacagtca 
      ctttttgat atagagcct caggaagtg ctaaggatt 
      tctgcaaaa ttaggcacc ggtattcaa tcggttatt 
      gaggcttta atcgagcga atttcgccc ccgaaagtg 
      gacagaacg gctccgttt cgagcccta cttttcgac 
      agttggttc gataaatac aggggggcc ttgtcgttg 
      atttatatt caagatggt gaaataaat gtgggggac 
      gaagtacag tcttgccac tcgcaaaaa acatacagc 
      atcaagagt ttatctctg ttacggcca caagaggtc 
      gttacgaaa aaactagtc gcgggtcag attggctta 
      atcggttgt aacatgagg acgagtaaa gaggcgttt 
      attggtgac accattcac ttgtcttcc tgccctgtt 
      gagcctctt caaggtttc gagccgccg cagccaatg 
      gtgttcgcc ggtgtttac ccaatggac caatcacag 
      cacgtcaat ttgaggagc gctatagat aaattgact 
      ttgaatgat tcggcggtt tcggttgat attgattcg 
      agtccggcc ctgggtcaa ggctggagg ctgggtttt 
      ttggggctc ttgcatttg gaggttttt tcgcagcgt 
      ttgcaacag gaatacggc gctgaaccg attctcaca 
      gcaccctct gtcacttat aaaattacg ttaaaagaa 
      accaaacaa aatattaag acggggcgc gagatattt 
      gttaataat ccagctttg tggcccgat actgtccac 
      attgaggag acttttgag ccgatggtg atcggaact 
      attatcacg cctgataag tatctgggg ccgattatg 
      tctctttgc atggaaaga cgaggtgtt caaagaaat 
      gccactaac atagacaac gatcgtgtt atgttacaa 
      tacgatttg ccgctttgc gaaataatt atagacttt 
      catgacagt ctaaaaact atttcttcc ggttatgcc 
      agttttgat tatgaagac aacggatac caaagctct 
      aatcttgtt aaattgcgg attttgttg aatgggaca 
      gcagttgaa gagttaagc aatattgtt catatctct 
      aaagctcaa tctattggt cggaaaatg gttctgaaa 
      ctgaaagaa atgatccct aggcaaatg atacaaatt 
      gctgtccag gctgtttgc aatggcaaa attttagct 
      agggagact atcaaagcg taccgtaaa gacgtaaca 
      gctaaactt tatggaggt gatgtaaca aggagaatg 
      aaactacta gcgcaacaa gccgccggg aaaaagaaa 
      atgcggatg gttgcaaac atttccttg cccagagag 
      acatttata gacgttttg aagaaataa
    mRNA sequence
    • >TC007205 RA
      agtatgttt aatttaatt aagaacata gtttacata 
      cacgcgaag tcaccaaaa tcgaaaata tgtcgtgtt 
      aagcgtgct gttattaca agaacgtat tgtgaagcc 
      cccatcgat tatactcaa attccaatc gaacgaata 
      agaaatttt agtataata gcccacgtt gaccatggt 
      aagagtact ctggccgac cgtctgctt gaattcaca 
      ggtgccata acgaaaaac agtggtcaa aaccaagtt 
      ttggataaa ttacaagtg gagcgcgaa cgaggaatc 
      acagtcaaa gcccaaagt gtgtcttta ttatataat 
      tacaaaggg aacgaatat ttattgaat ttaattgat 
      actccgggt catgtcgat ttttccagc gaggtctct 
      agatcactt tcagcctgc caaggggtt atcctagtg 
      gtggacgcc aatgacggg gtccaggcc caaactgtg 
      gcaaatttt tacctagct tttggcaaa gacctcacc 
      attgtgcct gttttaaat aaaattgat ttgaaaaat 
      gctgacccc gatagggtt accaaacag ttacagtca 
      ctttttgat atagagcct caggaagtg ctaaggatt 
      tctgcaaaa ttaggcacc ggtattcaa tcggttatt 
      gaggcttta atcgagcga atttcgccc ccgaaagtg 
      gacagaacg gctccgttt cgagcccta cttttcgac 
      agttggttc gataaatac aggggggcc ttgtcgttg 
      atttatatt caagatggt gaaataaat gtgggggac 
      gaagtacag tcttgccac tcgcaaaaa acatacagc 
      atcaagagt ttatctctg ttacggcca caagaggtc 
      gttacgaaa aaactagtc gcgggtcag attggctta 
      atcggttgt aacatgagg acgagtaaa gaggcgttt 
      attggtgac accattcac ttgtcttcc tgccctgtt 
      gagcctctt caaggtttc gagccgccg cagccaatg 
      gtgttcgcc ggtgtttac ccaatggac caatcacag 
      cacgtcaat ttgaggagc gctatagat aaattgact 
      ttgaatgat tcggcggtt tcggttgat attgattcg 
      agtccggcc ctgggtcaa ggctggagg ctgggtttt 
      ttggggctc ttgcatttg gaggttttt tcgcagcgt 
      ttgcaacag gaatacggc gctgaaccg attctcaca 
      gcaccctct gtcacttat aaaattacg ttaaaagaa 
      accaaacaa aatattaag acggggcgc gagatattt 
      gttaataat ccagctttg tggcccgat actgtccac 
      attgaggag acttttgag ccgatggtg atcggaact 
      attatcacg cctgataag tatctgggg ccgattatg 
      tctctttgc atggaaaga cgaggtgtt caaagaaat 
      gccactaac atagacaac gatcgtgtt atgttacaa 
      tacgatttg ccgctttgc gaaataatt atagacttt 
      catgacagt ctaaaaact atttcttcc ggttatgcc 
      agttttgat tatgaagac aacggatac caaagctct 
      aatcttgtt aaattgcgg attttgttg aatgggaca 
      gcagttgaa gagttaagc aatattgtt catatctct 
      aaagctcaa tctattggt cggaaaatg gttctgaaa 
      ctgaaagaa atgatccct aggcaaatg atacaaatt 
      gctgtccag gctgtttgc aatggcaaa attttagct 
      agggagact atcaaagcg taccgtaaa gacgtaaca 
      gctaaactt tatggaggt gatgtaaca aggagaatg 
      aaactacta gcgcaacaa gccgccggg aaaaagaaa 
      atgcggatg gttgcaaac atttccttg cccagagag 
      acatttata gacgttttg aagaaataa ttgtgattt 
      tttatttat aaaaattat aatttagaa taatacttt 
      tagtgtaat
    • >TC007205 RB
      agtatgttt aatttaatt aagaacata gtttacata 
      cacgcgaag tcaccaaaa tcgaaaata tgtcgtgtt 
      aagtggcgt gctgttatt acaagaacg tattgtgaa 
      gcccccatc gattatact caaattcca atcgaacga 
      ataagaaat tttagtata atagcccac gttgaccat 
      ggtaagagt actctggcc gaccgtctg cttgaattc 
      acaggtgcc ataacgaaa aacagtggt caaaaccaa 
      gttttggat aaattacaa gtggagcgc gaacgagga 
      atcacagtc aaagcccaa agtgtgtct ttattatat 
      aattacaaa gggaacgaa tatttattg aatttaatt 
      gatactccg ggtcatgtc gatttttcc agcgaggtc 
      tctagatca ctttcagcc tgccaaggg gttatccta 
      gtggtggac gccaatgac ggggtccag gcccaaact 
      gtggcaaat ttttaccta gcttttggc aaagacctc 
      accattgtg cctgtttta aataaaatt gatttgaaa 
      aatgctgac cccgatagg gttaccaaa cagttacag 
      tcacttttt gatatagag cctcaggaa gtgctaagg 
      atttctgca aaattaggc accggtatt caatcggtt 
      attgaggct ttaatcgag cgaatttcg cccccgaaa 
      gtggacaga acggctccg tttcgagcc ctacttttc 
      gacagttgg ttcgataaa tacaggggg gccttgtcg 
      ttgatttat attcaagat ggtgaaata aatgtgggg 
      gacgaagta cagtcttgc cactcgcaa aaaacatac 
      agcatcaag agtttatct ctgttacgg ccacaagag 
      gtcgttacg aaaaaacta gtcgcgggt cagattggc 
      ttaatcggt tgtaacatg aggacgagt aaagaggcg 
      tttattggt gacaccatt cacttgtct tcctgccct 
      gttgagcct cttcaaggt ttcgagccg ccgcagcca 
      atggtgttc gccggtgtt tacccaatg gaccaatca 
      cagcacgtc aatttgagg agcgctata gataaattg 
      actttgaat gattcggcg gtttcggtt gatattgat 
      tcgagtccg gccctgggt caaggctgg aggctgggt 
      tttttgggg ctcttgcat ttggaggtt ttttcgcag 
      cgtttgcaa caggaatac ggcgctgaa ccgattctc 
      acagcaccc tctgtcact tataaaatt acgttaaaa 
      gaaaccaaa caaaatatt aagacgggg cgcgagata 
      tttgttaat aatccagct ttgtggccc gatactgtc 
      cacattgag gagactttt gagccgatg gtgatcgga 
      actattatc acgcctgat aagtatctg gggccgatt 
      atgtctctt tgcatggaa agacgaggt gttcaaaga 
      aatgccact aacatagac aacgatcgt gttatgtta 
      caatacgat ttgccgctt tgcgaaata attatagac 
      tttcatgac agtctaaaa actatttct tccggttat 
      gccagtttt gattatgaa gacaacgga taccaaagc 
      tctaatctt gttaaattg cggattttg ttgaatggg 
      acagcagtt gaagagtta agcaatatt gttcatatc 
      tctaaagct caatctatt ggtcggaaa atggttctg 
      aaactgaaa gaaatgatc cctaggcaa atgatacaa 
      attgctgtc caggctgtt tgcaatggc aaaatttta 
      gctagggag actatcaaa gcgtaccgt aaagacgta 
      acagctaaa ctttatgga ggtgatgta acaaggaga 
      atgaaacta ctagcgcaa caagccgcc gggaaaaag 
      aaaatgcgg atggttgca aacatttcc ttgcccaga 
      gagacattt atagacgtt ttgaagaaa taattgtga 
      ttttttatt tataaaaat tataattta gaataatac 
      ttttagtgt aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY