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Gene information
General information
TC007204 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7563597..7566833
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007204
    iBeetle screen iB_01143

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi) mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    labium potentially posteriorstructure swollen - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007204 RA
      MKHLGFIIG LVFLQMTFS HSYNCDRTP QKAEDPKDN 
      IDKYVLEIS GNPETYSPE TTYTIALKS SPSNPITNH 
      FTEFMIVVE PENPSKIPE NVGTGDLTP VDPTVSKFT 
      PRCPNAVIQ KNNLAKSHV EILWKAPPA TTEGNNCIS 
      IKAMVFESS DSWFIDTGG LVKTLCQEE ENEDVQPPF 
      SEECCACHE AKYEIAFQG MWTRNTHPK DYPSNMWTT 
      KLGDVIGAS HKFGKAFWN YSEVASDGL KLLAEEGDT 
      SLLENELKE MVKTDDIRT IIKARELPY PNITGTSYT 
      VFRVDKDNH LISLVSKIT PSPDWIVGV ANLELCLQN 
      CSWVQSRNL NLYPWDVGT DDGTTYQSP DSPSASRQI 
      VRQIIASDE NYPFYRDDG EPIKPIAKL HITRQKLYD 
      KVCEEEDDK CKLSEWSDW TKCDCDTDK YKTRTKQFE 
      NPENEEYCS QNGKHPLEE TIECTPEEC ELARKCAQA 
      TWSDWGPCS VTCGTGTHK RNGTLPEED ENKETTNEE 
      SEEIPCVEE EPCYMPLCD DEETTLTSE SLVSDAPVD 
      CKVSKWSEW SSCDAREPC TGGNQFRER RILVHPQNG 
      GKPCPKRLK KVKYCEAPC AGNVDSKET LPSWGPARR 
      GRRVDCVMG PWSVWSPCS SNCGDSAVQ QRTRAILVY 
      PSRDGKKCE PRLEVRKCS LPYACRSRE YLQLSKH
    CDS sequence
    • >TC007204 RA
      atgaaacat ctcggtttc attattggc ttagttttc 
      ctccagatg accttttcc cacagctac aactgtgac 
      agaaccccg caaaaagcc gaagaccct aaagacaac 
      atcgataaa tatgttctg gaaatttcc ggaaatcct 
      gaaacctac tcacctgaa acaacctac acaattgcc 
      ttgaaatcg agtccctca aaccccatt acgaaccat 
      ttcactgag tttatgata gtggttgaa ccggaaaat 
      ccctcgaaa attcccgaa aacgtcggt acgggtgat 
      ttgacccca gttgatccc acagtgtcg aagtttaca 
      ccgaggtgc cccaatgcc gtcatacag aaaaataat 
      ttggcgaaa tcacacgtt gaaattttg tggaaggcg 
      ccaccggct accaccgag gggaataac tgtatttcc 
      atcaaggca atggtgttt gaatcgagc gatagctgg 
      tttattgac acgggaggg ttggtaaaa acgttgtgc 
      caagaagag gaaaatgag gatgtgcaa ccacccttt 
      tcggaggag tgttgtgcc tgccatgag gctaaatat 
      gaaatcgcg ttccaaggg atgtggact cgaaatacg 
      caccctaag gattacccg tccaatatg tggacgacc 
      aaactgggc gatgttatt ggagcgtct cacaagttt 
      ggcaaagcg ttttggaat tactcggaa gttgccagc 
      gatgggtta aaacttctg gctgaggaa ggcgatacc 
      tcgcttttg gaaaatgaa cttaaagaa atggtcaag 
      actgacgat attcgaaca attatcaaa gcccgagag 
      ttgccttac cctaacatt accggaact tcgtataca 
      gtgtttagg gtcgataaa gataatcat ttaatttct 
      ttagtatct aaaataacg ccttcgccg gattggatc 
      gtcggggtg gccaacttg gagctatgt ctccaaaac 
      tgcagttgg gtacaaagc cggaacttg aacttgtac 
      ccttgggac gttggtacc gacgatggt accacgtat 
      caatctcca gattcgcct tctgcttcc cggcaaatc 
      gtccgccag attattgca agtgatgaa aactacccc 
      ttttatcgc gacgacgga gagcccata aaaccaatc 
      gcaaaactc cacatcact cgccaaaaa ctatacgac 
      aaggtttgt gaagaagaa gacgacaaa tgtaaattg 
      agcgaatgg agcgactgg acaaaatgt gactgtgac 
      actgacaaa tacaaaaca cggaccaaa caattcgaa 
      aatcccgaa aacgaagaa tactgctcc cagaacggt 
      aaacaccca ttggaggaa accatcgaa tgcacgccc 
      gaagaatgc gaacttgcg cgaaaatgt gcccaagcc 
      acttggtca gactggggc ccctgttcg gtgacttgc 
      ggcacaggg acccacaaa cgcaacggc acccttccg 
      gaagaagac gaaaacaaa gagacaaca aacgaagaa 
      tctgaagaa attccatgc gttgaggaa gaaccctgc 
      tacatgcct ctgtgcgac gatgaagaa accaccctc 
      acttctgag agtctggtt tccgacgct ccagtcgac 
      tgcaaagtg agcaagtgg tctgagtgg agttcctgc 
      gacgctagg gaaccctgc actggcgga aaccaattc 
      cgggagcgc cggatcctc gttcatccc caaaatggg 
      ggaaagccg tgtcccaaa agattgaaa aaggtcaag 
      tattgtgag gctccctgt gccggtaat gtggactcg 
      aaagagacg ctaccatcg tggggtccg gctcgaagg 
      gggaggaga gttgattgt gtgatgggg ccatggtcg 
      gtgtggtcg ccgtgtagc agcaattgt ggtgattcg 
      gcggtgcag cagaggact agggccatt ttagtatat 
      ccgagcaga gatgggaag aagtgtgag ccgaggctt 
      gaggtcagg aagtgctcc ttgccgtat gcttgccgg 
      agcagggaa tatttacaa ttaagtaaa cattaa
    mRNA sequence
    • >TC007204 RA
      gtttcaggc gactgaaag cctgacaaa attaatttt 
      acacattga aaattggct taataactc atcggcttt 
      gccctgtgg tattttgtt ttcaccaaa tttctgaaa 
      aacgcattt tgcttactt gctgtttaa tctgaagtt 
      tattaaatt taatgaagc ggaaaacac gtgtcacga 
      agttctcga aatttatcg tcgtaaatg tttataaca 
      aagagataa ttaaagata tttgcaact gcgcatgat 
      acaaaatag aaaattcag tgaagaagc gtctttatc 
      ttaattagt tggtcgtcg aggctctct tcagatgct 
      gcacaatag tttttaaga acaacaatg aaacatctc 
      ggtttcatt attggctta gttttcctc cagatgacc 
      ttttcccac agctacaac tgtgacaga accccgcaa 
      aaagccgaa gaccctaaa gacaacatc gataaatat 
      gttctggaa atttccgga aatcctgaa acctactca 
      cctgaaaca acctacaca attgccttg aaatcgagt 
      ccctcaaac cccattacg aaccatttc actgagttt 
      atgatagtg gttgaaccg gaaaatccc tcgaaaatt 
      cccgaaaac gtcggtacg ggtgatttg accccagtt 
      gatcccaca gtgtcgaag tttacaccg aggtgcccc 
      aatgccgtc atacagaaa aataatttg gcgaaatca 
      cacgttgaa attttgtgg aaggcgcca ccggctacc 
      accgagggg aataactgt atttccatc aaggcaatg 
      gtgtttgaa tcgagcgat agctggttt attgacacg 
      ggagggttg gtaaaaacg ttgtgccaa gaagaggaa 
      aatgaggat gtgcaacca cccttttcg gaggagtgt 
      tgtgcctgc catgaggct aaatatgaa atcgcgttc 
      caagggatg tggactcga aatacgcac cctaaggat 
      tacccgtcc aatatgtgg acgaccaaa ctgggcgat 
      gttattgga gcgtctcac aagtttggc aaagcgttt 
      tggaattac tcggaagtt gccagcgat gggttaaaa 
      cttctggct gaggaaggc gatacctcg cttttggaa 
      aatgaactt aaagaaatg gtcaagact gacgatatt 
      cgaacaatt atcaaagcc cgagagttg ccttaccct 
      aacattacc ggaacttcg tatacagtg tttagggtc 
      gataaagat aatcattta atttcttta gtatctaaa 
      ataacgcct tcgccggat tggatcgtc ggggtggcc 
      aacttggag ctatgtctc caaaactgc agttgggta 
      caaagccgg aacttgaac ttgtaccct tgggacgtt 
      ggtaccgac gatggtacc acgtatcaa tctccagat 
      tcgccttct gcttcccgg caaatcgtc cgccagatt 
      attgcaagt gatgaaaac taccccttt tatcgcgac 
      gacggagag cccataaaa ccaatcgca aaactccac 
      atcactcgc caaaaacta tacgacaag gtttgtgaa 
      gaagaagac gacaaatgt aaattgagc gaatggagc 
      gactggaca aaatgtgac tgtgacact gacaaatac 
      aaaacacgg accaaacaa ttcgaaaat cccgaaaac 
      gaagaatac tgctcccag aacggtaaa cacccattg 
      gaggaaacc atcgaatgc acgcccgaa gaatgcgaa 
      cttgcgcga aaatgtgcc caagccact tggtcagac 
      tggggcccc tgttcggtg acttgcggc acagggacc 
      cacaaacgc aacggcacc cttccggaa gaagacgaa 
      aacaaagag acaacaaac gaagaatct gaagaaatt 
      ccatgcgtt gaggaagaa ccctgctac atgcctctg 
      tgcgacgat gaagaaacc accctcact tctgagagt 
      ctggtttcc gacgctcca gtcgactgc aaagtgagc 
      aagtggtct gagtggagt tcctgcgac gctagggaa 
      ccctgcact ggcggaaac caattccgg gagcgccgg 
      atcctcgtt catccccaa aatggggga aagccgtgt 
      cccaaaaga ttgaaaaag gtcaagtat tgtgaggct 
      ccctgtgcc ggtaatgtg gactcgaaa gagacgcta 
      ccatcgtgg ggtccggct cgaaggggg aggagagtt 
      gattgtgtg atggggcca tggtcggtg tggtcgccg 
      tgtagcagc aattgtggt gattcggcg gtgcagcag 
      aggactagg gccatttta gtatatccg agcagagat 
      gggaagaag tgtgagccg aggcttgag gtcaggaag 
      tgctccttg ccgtatgct tgccggagc agggaatat 
      ttacaatta agtaaacat taataataa agctcaaaa 
      acaaacaag tttgaactg tgtacttaa accacattt 
      ctaagcacg gaattgttt tcttcaaca ttctaaatc 
      ttattattt tatgttaaa ttgtccaat tggatcact 
      cgaaagaat tcaacacct acgtttgtt tctctgctt 
      tttcgaagg tttcttttt ttataaatt gccacaata 
      aagaagcat aacagcatt acagttcca aaaaacctc 
      attttatta tgttgtgtc cgttatttt aaaaatctc 
      gttgtatta cctgtgaat cacactact gttccacct 
      acaggttat cacaagtaa gtaacaata aaaatttat 
      aaccagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY