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Gene information
General information
TC007193 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7843781..7847973
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007193
    iBeetle screen iB_01141

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007193 RA
      MASIRIHQD QENRVPELR RKPGTTAQQ KRGVLGEID 
      SNKVNNAAL KGKQTKKPT VLGNRVENG QKNENKKNV 
      VVPVAQFEA FTVYEDDEY RARIDERLK LISKSNVYK 
      GTAEDRFIT KTELAEIER KKNLEKLKE ITAPQLKRK 
      SEIPMSIEK SDVENIELK EQTVSTSTN ARDVFFEME 
      EYRDSIFAY LKEHELRHR PKPGYIVKQ PDVTENMRA 
      VLIDWLVEV TEEYGMQTE TLYLAVNFI DRFLSYMSV 
      VRAKLQLVG TAAMFIASK YEEIFPPEV SEFVYITDD 
      TYDKHQMIR MEQLILRVL GFDLSVPTP LTFLNAICI 
      STKQTEKVK NLAMYLSES ALLEVEPYL QFLPSVVAS 
      SAIALSRHT LGEEAWPGD LQKYTGYNL KKLESCIGF 
      LYKMFAKAP SNPQHAIRD KYKAPKYMQ VSKISPSGE 
      MISFD
    CDS sequence
    • >TC007193 RA
      atggcatcg ataagaatt caccaggac caggaaaac 
      cgggtgccg gaattgcgc cgaaaaccg ggaacgact 
      gcccagcaa aaacggggc gttctgggc gagattgac 
      agtaataaa gttaataac gcagccctt aaaggaaaa 
      cagacgaag aagccaacc gttttggga aaccgggtg 
      gaaaatggg caaaaaaac gagaataag aagaatgtc 
      gtggttccg gtggcccag tttgaggcg tttactgtg 
      tatgaggat gatgaatac agagcgaga atcgatgaa 
      aggctcaaa cttatcagt aagagtaat gtgtataaa 
      gggacggcc gaggacagg tttatcacg aagacagaa 
      cttgccgaa attgaaagg aaaaagaat ttggagaaa 
      ttgaaggaa attactgcg cctcaactt aagagaaaa 
      tcggagatt ccgatgagc attgagaaa tcagatgtg 
      gagaacatt gagctcaaa gagcaaacg gtttccaca 
      agtaccaat gctagagat gtctttttt gaaatggag 
      gagtatagg gatagcatt tttgcttat ctcaaagaa 
      catgagctg cgacatcgg ccaaaaccc ggctacatc 
      gtgaaacag cctgacgta actgaaaac atgcgtgct 
      gttttgatc gattggctt gtcgaagtt actgaagag 
      tacggtatg caaacggaa accctttat ttggccgta 
      aatttcatc gaccgattt ttaagttac atgtctgta 
      gttagggcc aaattgcaa cttgtgggt acagctgca 
      atgtttatt gcttctaaa tacgaggaa atttttcca 
      ccagaagtc agcgaattc gtgtacatc actgacgac 
      acttacgac aaacaccaa atgattaga atggaacag 
      ttaattctg agggtgttg ggcttcgat ttgtcggtg 
      cccacacca cttactttc ctaaacgcc atttgcatt 
      tccacaaaa caaaccgaa aaagtgaag aatctcgcg 
      atgtattta agcgaatcg gcgcttctg gaggtcgaa 
      ccgtattta cagtttttg ccgtcagtt gtcgcgtcg 
      tcggcgata gctctcagc agacacact ttgggcgaa 
      gaagcgtgg cctggcgac ttgcaaaaa tacacagga 
      tataatttg aaaaaatta gaatcttgt attggattc 
      ttgtataaa atgttcgcc aaagcgcca tctaatcca 
      caacacgcc attcgggac aagtacaag gcgccaaag 
      tatatgcaa gtttctaag attagtccc agcggtgag 
      atgatttcg ttcgattaa
    mRNA sequence
    • >TC007193 RA
      tgacgttgc ttaaatctg ggctcccat tggtcccgt 
      ttgtcattt caacggtct ctcagctgt tcgtttccc 
      gcactctgc gctctttgc atctggccg ttaaaaaca 
      gtcgttttg tcggttagg ggtagcttg ggtgtttaa 
      gttggggtt cagtttggc cttcggttc agcaaactt 
      ttcgttttt cgggtatta gaaacttgc acgtgtgtt 
      agaggaatg gcatcgata agaattcac caggaccag 
      gaaaaccgg gtgccggaa ttgcgccga aaaccggga 
      acgactgcc cagcaaaaa cggggcgtt ctgggcgag 
      attgacagt aataaagtt aataacgca gcccttaaa 
      ggaaaacag acgaagaag ccaaccgtt ttgggaaac 
      cgggtggaa aatgggcaa aaaaacgag aataagaag 
      aatgtcgtg gttccggtg gcccagttt gaggcgttt 
      actgtgtat gaggatgat gaatacaga gcgagaatc 
      gatgaaagg ctcaaactt atcagtaag agtaatgtg 
      tataaaggg acggccgag gacaggttt atcacgaag 
      acagaactt gccgaaatt gaaaggaaa aagaatttg 
      gagaaattg aaggaaatt actgcgcct caacttaag 
      agaaaatcg gagattccg atgagcatt gagaaatca 
      gatgtggag aacattgag ctcaaagag caaacggtt 
      tccacaagt accaatgct agagatgtc ttttttgaa 
      atggaggag tatagggat agcattttt gcttatctc 
      aaagaacat gagctgcga catcggcca aaacccggc 
      tacatcgtg aaacagcct gacgtaact gaaaacatg 
      cgtgctgtt ttgatcgat tggcttgtc gaagttact 
      gaagagtac ggtatgcaa acggaaacc ctttatttg 
      gccgtaaat ttcatcgac cgattttta agttacatg 
      tctgtagtt agggccaaa ttgcaactt gtgggtaca 
      gctgcaatg tttattgct tctaaatac gaggaaatt 
      tttccacca gaagtcagc gaattcgtg tacatcact 
      gacgacact tacgacaaa caccaaatg attagaatg 
      gaacagtta attctgagg gtgttgggc ttcgatttg 
      tcggtgccc acaccactt actttccta aacgccatt 
      tgcatttcc acaaaacaa accgaaaaa gtgaagaat 
      ctcgcgatg tatttaagc gaatcggcg cttctggag 
      gtcgaaccg tatttacag tttttgccg tcagttgtc 
      gcgtcgtcg gcgatagct ctcagcaga cacactttg 
      ggcgaagaa gcgtggcct ggcgacttg caaaaatac 
      acaggatat aatttgaaa aaattagaa tcttgtatt 
      ggattcttg tataaaatg ttcgccaaa gcgccatct 
      aatccacaa cacgccatt cgggacaag tacaaggcg 
      ccaaagtat atgcaagtt tctaagatt agtcccagc 
      ggtgagatg atttcgttc gattaaaag actgctcta 
      cttcgtagg gaacaaagg ccttgcgtt tttttttct 
      ggtctagtt tttattatt agatttatt aattagatg 
      gtaataatt tatagtaag ctgtgctgt ggtgtccca 
      ttcatgtgt actgtcgac catcaatat tattttagt 
      ttgcatggt tttatttac tgtctttta tgtgttatt 
      ttatttgga tttacgtat tttgaattt atactctcg 
      ttgaaacta ccttgtttc ctaaacatt gtacagttt 
      atttatttg ttataagta gttaagaat caaacaaac 
      gaattgctg ccattcctt tataacaaa atggacgaa 
      attgtcaat aacaatttt tgatatttt tctatacgc 
      aataaattg tggaactac aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY