Status
Please wait ...
Gene information
General information
TC007189 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7874561..7876123
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007189
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01140

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007189 RA
      MAIQTKLVI IFLLSSLLY GREVSADSD VGLNTVEMI 
      EKHGYVCET HYITTEDGY ILTYHRIPH GKNNDNSTK 
      RPAVLLMHG LISSSADYV NMGPNNSLA YILADIGYD 
      VWLGNARGN GWSRNHTTL DIVADAEKF FDFSWHEIG 
      YYDLPAAID YILDVNGDD SIYYVGHSQ GTTAFMVLG 
      STRPEYNSK IKIASLMGP ASYMEHQST TLLVGLSKY 
      IFELEKVVK KYTIFEIPL LAQLRKFAS DFCSNPDSL 
      NICEDVIGL IGGQDKPQF DFEKFPVIL TNAPSNAAM 
      KQLYHYGQL IKNGGFSQF DFGSKEKNK EIYGTDTPP 
      AYDLSKISA PVAVYYGKN DQLVNYLDA QTVVKNLGN 
      VANDYFIPY DLFDHLDFI FAKDVVNML YVELIKVMQ 
      KY
    CDS sequence
    • >TC007189 RA
      atggctatc caaaccaaa ctggtaatc attttcctc 
      ctctcaagt ctcctctat ggacgtgaa gtgtccgca 
      gactcggac gtgggtttg aacacagtt gaaatgata 
      gaaaagcat ggctacgtg tgtgaaacc cactacata 
      acaaccgaa gatggttac atcttaaca taccacaga 
      ataccacat ggtaaaaac aacgataat tcgaccaaa 
      agaccggcc gttttgctc atgcatggt ctcatatcg 
      tcttcggcc gattacgta aatatgggt ccgaacaat 
      agtctcgcc tatattttg gccgacatc ggatatgat 
      gtatggctt ggtaatgct agagggaac ggatggtcg 
      cgaaatcac acaactctg gatatagta gcagatgct 
      gagaagttt ttcgatttc agttggcat gaaatcggg 
      tattacgac ctgcccgct gctatagat tacatcttg 
      gatgtaaac ggtgacgat agtatttat tatgtagga 
      cactctcaa ggtacaact gccttcatg gtcttgggg 
      tccactagg ccagaatat aacagcaaa atcaaaatt 
      gcctcactt atggggcct gcctcgtat atggagcat 
      cagtccact acgctttta gtaggtctt tcaaaatat 
      atctttgag ttggagaaa gtagtaaag aaatacaca 
      atatttgaa ataccgctt ctggcacaa ctacgaaag 
      tttgcgtct gatttttgc tcaaatccc gacagtctc 
      aacatctgc gaagatgtt atcggtctc ataggtgga 
      caagataaa ccgcaattt gacttcgaa aaattccct 
      gtaattctt acaaatgca ccctcaaac gcagccatg 
      aaacagctt tatcactat ggccaactt atcaaaaat 
      ggtggattt tcgcagttt gattttggc agtaaagaa 
      aaaaacaag gaaatttat ggaaccgat acacccccg 
      gcctatgat ttgtcgaaa atttcagca cctgtggct 
      gtttattat ggaaaaaat gaccagctg gtgaactac 
      ttggatgcg caaactgta gtaaagaat cttggaaat 
      gtagctaat gattatttc ataccgtac gacctattc 
      gatcacttg gacttcatt tttgcaaag gatgttgta 
      aatatgctc tatgtagag ctaattaaa gttatgcaa 
      aagtactga
    mRNA sequence
    • >TC007189 RA
      agaaatcag atgcataaa agaaaaaat aaatcgata 
      aagttccat tgtagatgt tataaattc gtttagtgg 
      tcctaatcc tctcatcag tgatccgca atggctatc 
      caaaccaaa ctggtaatc attttcctc ctctcaagt 
      ctcctctat ggacgtgaa gtgtccgca gactcggac 
      gtgggtttg aacacagtt gaaatgata gaaaagcat 
      ggctacgtg tgtgaaacc cactacata acaaccgaa 
      gatggttac atcttaaca taccacaga ataccacat 
      ggtaaaaac aacgataat tcgaccaaa agaccggcc 
      gttttgctc atgcatggt ctcatatcg tcttcggcc 
      gattacgta aatatgggt ccgaacaat agtctcgcc 
      tatattttg gccgacatc ggatatgat gtatggctt 
      ggtaatgct agagggaac ggatggtcg cgaaatcac 
      acaactctg gatatagta gcagatgct gagaagttt 
      ttcgatttc agttggcat gaaatcggg tattacgac 
      ctgcccgct gctatagat tacatcttg gatgtaaac 
      ggtgacgat agtatttat tatgtagga cactctcaa 
      ggtacaact gccttcatg gtcttgggg tccactagg 
      ccagaatat aacagcaaa atcaaaatt gcctcactt 
      atggggcct gcctcgtat atggagcat cagtccact 
      acgctttta gtaggtctt tcaaaatat atctttgag 
      ttggagaaa gtagtaaag aaatacaca atatttgaa 
      ataccgctt ctggcacaa ctacgaaag tttgcgtct 
      gatttttgc tcaaatccc gacagtctc aacatctgc 
      gaagatgtt atcggtctc ataggtgga caagataaa 
      ccgcaattt gacttcgaa aaattccct gtaattctt 
      acaaatgca ccctcaaac gcagccatg aaacagctt 
      tatcactat ggccaactt atcaaaaat ggtggattt 
      tcgcagttt gattttggc agtaaagaa aaaaacaag 
      gaaatttat ggaaccgat acacccccg gcctatgat 
      ttgtcgaaa atttcagca cctgtggct gtttattat 
      ggaaaaaat gaccagctg gtgaactac ttggatgcg 
      caaactgta gtaaagaat cttggaaat gtagctaat 
      gattatttc ataccgtac gacctattc gatcacttg 
      gacttcatt tttgcaaag gatgttgta aatatgctc 
      tatgtagag ctaattaaa gttatgcaa aagtactga 
      ataaatttt tacatagga tcgcttatg tgttttttt 
      cttcctcac atgaataca cttaaaatt tagtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY