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Gene information
General information
TC007182 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7891878..7893263
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007182
    iBeetle screen iB_01138

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007182 RA
      MNLLVVFLS FTLLAVSSA NPLAKHDED ADLTVPELI 
      TKYGYPAEE HHVITEDGY ILTLHRIPH GKNPNKSLG 
      KIAFLQHGV LSSSADWII TGPSHGLGY ILADEGYDV 
      WMGNARGNK LSRNHTSLN PDKDSQFWN FSWHQIGLI 
      DLPTMIDYV LEVTNQTEL YYIGHSQGT TTFYVMTSM 
      LPEYNAKIK AQFSLAPIA YMNHMTSPL LHIIAFWTG 
      PLDLLLQLI GINEFLPSN EFMALVGDI LCGDDDITQ 
      ILCSNVLFA ICGFSPSEM NATILPALM GHTPAGASV 
      MQIIHYGQE VISGGFRQY DFGLGNWDH YHSWTPPLY 
      DLSQITTPV YLFYSHNDW LAAEQDVLR LCKGLGNAC 
      AGKFIVSDN GFNHLDYMF GIHAPEYVY NRVISLMAR 
      H
    CDS sequence
    • >TC007182 RA
      atgaattta ctagttgtt ttcctgagt tttacgctc 
      ttggcagtt tcctctgct aaccctttg gcaaaacat 
      gatgaagac gccgatctc acagtcccc gaactaatc 
      accaaatac ggctaccca gccgaggaa catcatgta 
      ataaccgag gacggttac atcctaacc ctccaccgg 
      atcccccac ggtaaaaat ccgaacaaa agcttgggc 
      aaaattgcg ttcctccaa catggtgtg ctgagttct 
      tcggctgat tggattatc actggacca agccatggc 
      cttggctac atcctagct gatgaaggc tacgatgtt 
      tggatgggt aacgctcgc gggaacaaa ctttcccga 
      aaccacacc tctctgaac cctgacaaa gattcccaa 
      ttttggaac ttcagttgg caccagatt gggttaatt 
      gatctgccc acaatgatc gactatgtt ttggaagtt 
      acaaaccaa accgaactt tactacatc ggccactct 
      caaggaacc actacgttc tacgttatg acctccatg 
      ctcccggaa tacaacgcc aaaatcaaa gcgcagttc 
      agtctcgca cccattgct tacatgaac cacatgact 
      agcccgctt ttgcacatt attgccttc tggactgga 
      cctctcgac ctcctcctg caacttatt ggaatcaac 
      gagtttctt ccaagcaac gaatttatg gctttggtc 
      ggagacatt ctctgtggc gacgacgac atcacccaa 
      attctctgc agcaatgtt cttttcgcc atttgcggt 
      ttcagtcca tctgagatg aacgcaaca attctccca 
      gctttgatg ggtcatact cccgccgga gcttcagtc 
      atgcagata atccattat gggcaagaa gtcatttcg 
      ggaggtttc cgtcaatat gattttggt ttgggaaat 
      tgggaccat taccactcc tggactccc ccattgtac 
      gacttgtcc caaatcaca actccggtt tatctgttc 
      tacagtcat aacgactgg ctggctgct gaacaagac 
      gttctacga ctttgcaaa ggattggga aatgcctgt 
      gcgggcaaa ttcatagtg tcagataat ggcttcaac 
      cacttggac tacatgttt ggaattcat gctccggaa 
      tacgtttac aacagagtt atcagtttg atggctagg 
      cattag
    mRNA sequence
    • >TC007182 RA
      acactttac ttcttcctt ctaaaatct tattaccta 
      caatgaatt tactagttg ttttcctga gttttacgc 
      tcttggcag tttcctctg ctaaccctt tggcaaaac 
      atgatgaag acgccgatc tcacagtcc ccgaactaa 
      tcaccaaat acggctacc cagccgagg aacatcatg 
      taataaccg aggacggtt acatcctaa ccctccacc 
      ggatccccc acggtaaaa atccgaaca aaagcttgg 
      gcaaaattg cgttcctcc aacatggtg tgctgagtt 
      cttcggctg attggatta tcactggac caagccatg 
      gccttggct acatcctag ctgatgaag gctacgatg 
      tttggatgg gtaacgctc gcgggaaca aactttccc 
      gaaaccaca cctctctga accctgaca aagattccc 
      aattttgga acttcagtt ggcaccaga ttgggttaa 
      ttgatctgc ccacaatga tcgactatg ttttggaag 
      ttacaaacc aaaccgaac tttactaca tcggccact 
      ctcaaggaa ccactacgt tctacgtta tgacctcca 
      tgctcccgg aatacaacg ccaaaatca aagcgcagt 
      tcagtctcg cacccattg cttacatga accacatga 
      ctagcccgc ttttgcaca ttattgcct tctggactg 
      gacctctcg acctcctcc tgcaactta ttggaatca 
      acgagtttc ttccaagca acgaattta tggctttgg 
      tcggagaca ttctctgtg gcgacgacg acatcaccc 
      aaattctct gcagcaatg ttcttttcg ccatttgcg 
      gtttcagtc catctgaga tgaacgcaa caattctcc 
      cagctttga tgggtcata ctcccgccg gagcttcag 
      tcatgcaga taatccatt atgggcaag aagtcattt 
      cgggaggtt tccgtcaat atgattttg gtttgggaa 
      attgggacc attaccact cctggactc ccccattgt 
      acgacttgt cccaaatca caactccgg tttatctgt 
      tctacagtc ataacgact ggctggctg ctgaacaag 
      acgttctac gactttgca aaggattgg gaaatgcct 
      gtgcgggca aattcatag tgtcagata atggcttca 
      accacttgg actacatgt ttggaattc atgctccgg 
      aatacgttt acaacagag ttatcagtt tgatggcta 
      ggcattagg tgatggttg tgtaaatgt taactactg 
      tacctgtat ctactatta ttaaaatca gtactatgt 
      acttgaacg cttattatt aaacaattg aaagtaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY