Status
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Gene information
General information
TC007163 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8312858..8320690
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007163
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01135

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    Pupal morphology
    wings complete not present - Number of animals showing the phenotype: 5

    Adult morphology
    wings decreased - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007163 RA
      MDENIPPFS GDFNLNELQ GIDDILTNC ENELMKSNE 
LFPEDALFS QLDPLLPMD DVSFDLGNI SETTFLDPQ 
TLSPLKQQN PVFNSQNFQ SNQFANAQN RQAFTTLNK 
PDVQHVQNP TAVIISQSQ PLVYSSLPI QQNNQQHII 
LQPNPKSEQ ITKTQPVIV QNLNQIPSD KMQQLLLQA 
KIVKTNPQP TVMYATTAA SPQPSLHTL VNSGTQILT 
TGIPLVLDT ENKVPINRI PQGTGKEPK VKEVKRSAH 
NAIERKYRT SINDKIVEL KNIVVGTEA KLNKSGILK 
KTIEYIRFL QNSNTRLKQ ENMALKMAA RQNTLKDLL 
TTGKNMEYP PQDTPPHSD ISSSSPDHS LPSSPECST 
FIKDESDEE TVGISKGLL DQTRITMFM FMLAVISFN 
PFGIVLKQF ASSDQEVSS VGRNILSSG DGQTPFSFG 
SSLMLWFIN LMVLGFCLA KMFVYGDPV IPSSSKEAQ 
KFWRHRRQA DICIKAGDK LEAKQELRR CLQTFGITL 
ATSRFELVL SLCWQVFRQ VMHRLWIGR WLSRHVGGF 
FVDGATRYE ALTSCRELS LVYHDLHQL QLVDGPEET 
CHLLGLTTA LSALNLAEA AKPKMKSVQ MIDIYVALA 
LRIKASCLN ILQGIQRYY LGLAKLAST NSCDPIPPR 
LQWLLTPHG FKFFLSQRF LYDAKATNL PFSCIGNVS 
DPLSFAMKS YREHLLETA LQTLTTPGN KNDVYEDKK 
TDITDVSTY VHLLLENVA TDVHTVFNS SQAHSNCDE 
IAHWWTSVV DVASHWLLG EENIDDVYK KVENIPESL 
LHLENPLPK AVIGAFVAR KNYLTAGSS VSHKKILKQ 
CDHASQLLT DSLTYSSCK SQDNLILLC QLLVCDWIL 
ETRTSLWED SVEASTTPI PVPNNVLTA FQKDLSSLR 
TLAQNIPSA LPRVFLYEA TARLMAGAA PGRTQQLLD 
RSLRQRHAR SSMICGKGD KNQQDVGGE RQHATALYM 
ACKHLPGQL MSSPGERAG MLVEAAKTM ERIGDKKKL 
QECYKLMKT LGANSATN
    CDS sequence
    • >TC007163 RA
      atggatgag aacataccc ccatttagt ggggatttt 
aatctaaat gagttacaa ggtatagac gatatccta 
acaaactgc gaaaacgaa ttgatgaag agcaatgag 
ctgttccca gaagatgcc ctattttcc cagcttgac 
cctctctta cccatggac gatgtcagc ttcgatttg 
ggaaatatc agcgaaact acgtttctg gaccctcag 
accctctca cctctcaag cagcaaaat cctgtgttc 
aactcccag aattttcaa tcaaatcag tttgcaaac 
gcccaaaac cggcaagct ttcacaaca ttgaacaag 
ccagacgtg cagcacgtc caaaatcca actgctgtt 
atcatttcc caatcacag cctcttgtt tactccagt 
ttaccaatc cagcaaaac aatcagcaa catataatc 
ctacaacca aatcccaaa tccgaacaa atcacgaaa 
acgcagcct gttattgta caaaaccta aaccaaatc 
ccttctgat aaaatgcaa cagctgttg ctccaagcg 
aaaattgtt aaaactaat ccccaacct actgtcatg 
tacgctact acagccgct tcacctcaa ccttctttg 
catactttg gtgaatagc ggcactcag attttgacc 
actggaatc cctttggtc ctagacact gagaataaa 
gttcccatc aataggatt cctcaaggg actgggaag 
gagcccaag gttaaagag gtgaagagg agtgctcat 
aatgctatc gagaggaag tacaggacc agcattaat 
gataagatt gtcgaattg aaaaatatc gtcgtgggg 
actgaagca aagttaaac aaatccgga attttgaag 
aaaacaatc gaatacatc cgcttttta caaaactca 
aacacacgt ctgaaacaa gaaaacatg gctctgaaa 
atggccgct cgtcaaaac actctcaaa gatttgcta 
acgacgggc aaaaatatg gaatatccc cctcaagac 
actcctccc cattcggac atttcgtcc tcatcaccc 
gaccactcc ttaccatca agtcccgag tgttcgaca 
tttattaag gatgagagt gacgaagag accgttggg 
atttccaaa ggtcttttg gaccaaacc cgaattaca 
atgttcatg tttatgttg gcggtcatt tcctttaat 
ccttttggt atcgtcctg aaacagttt gcttcctca 
gatcaggag gtgtcaagt gttggacga aatattttg 
tcgtcaggt gacggccaa acgccgttt tcgtttggt 
tcctcgttg atgctttgg ttcataaat ctgatggtt 
ttgggtttt tgcttggcc aaaatgttt gtttacggc 
gaccctgtt attccgagt agttcaaag gaggcgcag 
aaattctgg cgccatcga agacaggcc gatatttgt 
atcaaagcg ggtgataaa ctcgaagca aagcaggaa 
cttcggagg tgtctccaa actttcggt ataacatta 
gcaacgagt cgtttcgaa ctcgtgttg tcgctgtgc 
tggcaagtg ttccgtcaa gttatgcac cggttatgg 
atcgggcgg tggttgtct agacacgtc ggaggtttt 
ttcgtcgac ggagccacg cgatacgaa gctctgact 
tcctgccgc gaactttct ttggtttat cacgactta 
catcagctc caattggtg gatgggccg gaagagact 
tgtcatctc ctcggcttg acaactgca ttaagtgca 
ctgaacttg gccgaagcg gcgaaaccc aaaatgaag 
tcggttcaa atgatcgac atttacgtc gccttggct 
ttgaggatt aaagcctcg tgtttgaat attttacaa 
gggatccag agatattac ctaggtctg gcgaagtta 
gcatcgacc aattcttgc gatccgatt ccgccgagg 
ttgcagtgg ctgttgact cctcacggg ttcaagttt 
ttcctttcg cagaggttt ctctacgat gccaaagcc 
acgaatttg ccttttagt tgtattggc aacgtttcc 
gatcccttg tccttcgct atgaagtcg tatagagaa 
catttactg gaaactgca ctacaaact ttaacgact 
cccggtaat aaaaatgac gtatatgaa gacaaaaag 
acggacatt acggacgtg tcaacttac gtccatttg 
ttgcttgaa aatgttgcc accgatgta cacacagtt 
tttaattcg agtcaggcg cacagtaat tgtgacgaa 
atagcccac tggtggact tcagtcgtc gatgtggcc 
tctcattgg ctccttgga gaggagaac atcgatgat 
gtgtataaa aaagttgaa aatatacca gaatcattg 
ttacatctc gaaaatccg cttcctaaa gccgtgatt 
ggcgccttt gtggctagg aaaaattat cttactgct 
ggatcgagt gtttcgcat aagaaaatt ctgaagcag 
tgtgatcac gccagtcag ttgttaacc gacagtctg 
acctattcc agctgcaaa tcacaggat aatttgatt 
ttgttgtgt caactgctg gtgtgcgac tggatcctt 
gaaaccaga acgtccctt tgggaggac tcagttgag 
gcatcaaca acgccgatc ccagtccct aacaatgtc 
ctaacggcg ttccaaaaa gatttatct tcattgcga 
accctagcc caaaatatt cccagcgcg ttgcctcgg 
gtctttctg tacgaagct accgcccgc ttgatggcg 
ggtgcagcc ccaggccgc actcagcag ctcctcgat 
cgcagtttg cgacagcgt cacgctcgt tcgtcgatg 
atttgcggc aaaggcgat aaaaaccaa caggatgtt 
ggaggagag cgccaacac gcgacggct ttgtatatg 
gcttgcaaa catttgccc ggtcaattg atgtcatcg 
cctggagaa agggccgga atgttggtc gaagcagcg 
aaaactatg gaacgaatc ggcgataaa aagaaactg 
caggaatgc tataagctg atgaagaca ctcggtgcc 
aactcggcc acgaattag
    mRNA sequence
    • >TC007163 RA
      tttttaaaa ataatgcat ctagcccca tctatacct 
cagtcacca aactactta caattaaga actgataaa 
cgctaaaaa atctgtaaa agtgtatgg tgatgttta 
tgtgttttt gtgttacat ctgtggtta cagttgact 
caaaaaacg caaaaaatg gatgagaac ataccccca 
tttagtggg gattttaat ctaaatgag ttacaaggt 
atagacgat atcctaaca aactgcgaa aacgaattg 
atgaagagc aatgagctg ttcccagaa gatgcccta 
ttttcccag cttgaccct ctcttaccc atggacgat 
gtcagcttc gatttggga aatatcagc gaaactacg 
tttctggac cctcagacc ctctcacct ctcaagcag 
caaaatcct gtgttcaac tcccagaat tttcaatca 
aatcagttt gcaaacgcc caaaaccgg caagctttc 
acaacattg aacaagcca gacgtgcag cacgtccaa 
aatccaact gctgttatc atttcccaa tcacagcct 
cttgtttac tccagttta ccaatccag caaaacaat 
cagcaacat ataatccta caaccaaat cccaaatcc 
gaacaaatc acgaaaacg cagcctgtt attgtacaa 
aacctaaac caaatccct tctgataaa atgcaacag 
ctgttgctc caagcgaaa attgttaaa actaatccc 
caacctact gtcatgtac gctactaca gccgcttca 
cctcaacct tctttgcat actttggtg aatagcggc 
actcagatt ttgaccact ggaatccct ttggtccta 
gacactgag aataaagtt cccatcaat aggattcct 
caagggact gggaaggag cccaaggtt aaagaggtg 
aagaggagt gctcataat gctatcgag aggaagtac 
aggaccagc attaatgat aagattgtc gaattgaaa 
aatatcgtc gtggggact gaagcaaag ttaaacaaa 
tccggaatt ttgaagaaa acaatcgaa tacatccgc 
tttttacaa aactcaaac acacgtctg aaacaagaa 
aacatggct ctgaaaatg gccgctcgt caaaacact 
ctcaaagat ttgctaacg acgggcaaa aatatggaa 
tatccccct caagacact cctccccat tcggacatt 
tcgtcctca tcacccgac cactcctta ccatcaagt 
cccgagtgt tcgacattt attaaggat gagagtgac 
gaagagacc gttgggatt tccaaaggt cttttggac 
caaacccga attacaatg ttcatgttt atgttggcg 
gtcatttcc tttaatcct tttggtatc gtcctgaaa 
cagtttgct tcctcagat caggaggtg tcaagtgtt 
ggacgaaat attttgtcg tcaggtgac ggccaaacg 
ccgttttcg tttggttcc tcgttgatg ctttggttc 
ataaatctg atggttttg ggtttttgc ttggccaaa 
atgtttgtt tacggcgac cctgttatt ccgagtagt 
tcaaaggag gcgcagaaa ttctggcgc catcgaaga 
caggccgat atttgtatc aaagcgggt gataaactc 
gaagcaaag caggaactt cggaggtgt ctccaaact 
ttcggtata acattagca acgagtcgt ttcgaactc 
gtgttgtcg ctgtgctgg caagtgttc cgtcaagtt 
atgcaccgg ttatggatc gggcggtgg ttgtctaga 
cacgtcgga ggttttttc gtcgacgga gccacgcga 
tacgaagct ctgacttcc tgccgcgaa ctttctttg 
gtttatcac gacttacat cagctccaa ttggtggat 
gggccggaa gagacttgt catctcctc ggcttgaca 
actgcatta agtgcactg aacttggcc gaagcggcg 
aaacccaaa atgaagtcg gttcaaatg atcgacatt 
tacgtcgcc ttggctttg aggattaaa gcctcgtgt 
ttgaatatt ttacaaggg atccagaga tattaccta 
ggtctggcg aagttagca tcgaccaat tcttgcgat 
ccgattccg ccgaggttg cagtggctg ttgactcct 
cacgggttc aagtttttc ctttcgcag aggtttctc 
tacgatgcc aaagccacg aatttgcct tttagttgt 
attggcaac gtttccgat cccttgtcc ttcgctatg 
aagtcgtat agagaacat ttactggaa actgcacta 
caaacttta acgactccc ggtaataaa aatgacgta 
tatgaagac aaaaagacg gacattacg gacgtgtca 
acttacgtc catttgttg cttgaaaat gttgccacc 
gatgtacac acagttttt aattcgagt caggcgcac 
agtaattgt gacgaaata gcccactgg tggacttca 
gtcgtcgat gtggcctct cattggctc cttggagag 
gagaacatc gatgatgtg tataaaaaa gttgaaaat 
ataccagaa tcattgtta catctcgaa aatccgctt 
cctaaagcc gtgattggc gcctttgtg gctaggaaa 
aattatctt actgctgga tcgagtgtt tcgcataag 
aaaattctg aagcagtgt gatcacgcc agtcagttg 
ttaaccgac agtctgacc tattccagc tgcaaatca 
caggataat ttgattttg ttgtgtcaa ctgctggtg 
tgcgactgg atccttgaa accagaacg tccctttgg 
gaggactca gttgaggca tcaacaacg ccgatccca 
gtccctaac aatgtccta acggcgttc caaaaagat 
ttatcttca ttgcgaacc ctagcccaa aatattccc 
agcgcgttg cctcgggtc tttctgtac gaagctacc 
gcccgcttg atggcgggt gcagcccca ggccgcact 
cagcagctc ctcgatcgc agtttgcga cagcgtcac 
gctcgttcg tcgatgatt tgcggcaaa ggcgataaa 
aaccaacag gatgttgga ggagagcgc caacacgcg 
acggctttg tatatggct tgcaaacat ttgcccggt 
caattgatg tcatcgcct ggagaaagg gccggaatg 
ttggtcgaa gcagcgaaa actatggaa cgaatcggc 
gataaaaag aaactgcag gaatgctat aagctgatg 
aagacactc ggtgccaac tcggccacg aattagcgt 
agttaattt ttatagcgg gcgtaaaac gtgatgttt 
atgtcgggt tttgatttt aattatttg atgtgacaa 
tatttattt tgtacaaat tctttttaa taaatagtg 
tgtgagaag

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY