Status
Please wait ...
Gene information
General information
TC007163 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 8312858..8320690
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007163
    iBeetle screen iB_01135
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    wings complete not present - Number of animals showing the phenotype: 5

    Adult morphology
    wings decreased - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007163 RA
      MDENIPPFS GDFNLNELQ GIDDILTNC ENELMKSNE 
      LFPEDALFS QLDPLLPMD DVSFDLGNI SETTFLDPQ 
      TLSPLKQQN PVFNSQNFQ SNQFANAQN RQAFTTLNK 
      PDVQHVQNP TAVIISQSQ PLVYSSLPI QQNNQQHII 
      LQPNPKSEQ ITKTQPVIV QNLNQIPSD KMQQLLLQA 
      KIVKTNPQP TVMYATTAA SPQPSLHTL VNSGTQILT 
      TGIPLVLDT ENKVPINRI PQGTGKEPK VKEVKRSAH 
      NAIERKYRT SINDKIVEL KNIVVGTEA KLNKSGILK 
      KTIEYIRFL QNSNTRLKQ ENMALKMAA RQNTLKDLL 
      TTGKNMEYP PQDTPPHSD ISSSSPDHS LPSSPECST 
      FIKDESDEE TVGISKGLL DQTRITMFM FMLAVISFN 
      PFGIVLKQF ASSDQEVSS VGRNILSSG DGQTPFSFG 
      SSLMLWFIN LMVLGFCLA KMFVYGDPV IPSSSKEAQ 
      KFWRHRRQA DICIKAGDK LEAKQELRR CLQTFGITL 
      ATSRFELVL SLCWQVFRQ VMHRLWIGR WLSRHVGGF 
      FVDGATRYE ALTSCRELS LVYHDLHQL QLVDGPEET 
      CHLLGLTTA LSALNLAEA AKPKMKSVQ MIDIYVALA 
      LRIKASCLN ILQGIQRYY LGLAKLAST NSCDPIPPR 
      LQWLLTPHG FKFFLSQRF LYDAKATNL PFSCIGNVS 
      DPLSFAMKS YREHLLETA LQTLTTPGN KNDVYEDKK 
      TDITDVSTY VHLLLENVA TDVHTVFNS SQAHSNCDE 
      IAHWWTSVV DVASHWLLG EENIDDVYK KVENIPESL 
      LHLENPLPK AVIGAFVAR KNYLTAGSS VSHKKILKQ 
      CDHASQLLT DSLTYSSCK SQDNLILLC QLLVCDWIL 
      ETRTSLWED SVEASTTPI PVPNNVLTA FQKDLSSLR 
      TLAQNIPSA LPRVFLYEA TARLMAGAA PGRTQQLLD 
      RSLRQRHAR SSMICGKGD KNQQDVGGE RQHATALYM 
      ACKHLPGQL MSSPGERAG MLVEAAKTM ERIGDKKKL 
      QECYKLMKT LGANSATN
    CDS sequence
    • >TC007163 RA
      atggatgag aacataccc ccatttagt ggggatttt 
      aatctaaat gagttacaa ggtatagac gatatccta 
      acaaactgc gaaaacgaa ttgatgaag agcaatgag 
      ctgttccca gaagatgcc ctattttcc cagcttgac 
      cctctctta cccatggac gatgtcagc ttcgatttg 
      ggaaatatc agcgaaact acgtttctg gaccctcag 
      accctctca cctctcaag cagcaaaat cctgtgttc 
      aactcccag aattttcaa tcaaatcag tttgcaaac 
      gcccaaaac cggcaagct ttcacaaca ttgaacaag 
      ccagacgtg cagcacgtc caaaatcca actgctgtt 
      atcatttcc caatcacag cctcttgtt tactccagt 
      ttaccaatc cagcaaaac aatcagcaa catataatc 
      ctacaacca aatcccaaa tccgaacaa atcacgaaa 
      acgcagcct gttattgta caaaaccta aaccaaatc 
      ccttctgat aaaatgcaa cagctgttg ctccaagcg 
      aaaattgtt aaaactaat ccccaacct actgtcatg 
      tacgctact acagccgct tcacctcaa ccttctttg 
      catactttg gtgaatagc ggcactcag attttgacc 
      actggaatc cctttggtc ctagacact gagaataaa 
      gttcccatc aataggatt cctcaaggg actgggaag 
      gagcccaag gttaaagag gtgaagagg agtgctcat 
      aatgctatc gagaggaag tacaggacc agcattaat 
      gataagatt gtcgaattg aaaaatatc gtcgtgggg 
      actgaagca aagttaaac aaatccgga attttgaag 
      aaaacaatc gaatacatc cgcttttta caaaactca 
      aacacacgt ctgaaacaa gaaaacatg gctctgaaa 
      atggccgct cgtcaaaac actctcaaa gatttgcta 
      acgacgggc aaaaatatg gaatatccc cctcaagac 
      actcctccc cattcggac atttcgtcc tcatcaccc 
      gaccactcc ttaccatca agtcccgag tgttcgaca 
      tttattaag gatgagagt gacgaagag accgttggg 
      atttccaaa ggtcttttg gaccaaacc cgaattaca 
      atgttcatg tttatgttg gcggtcatt tcctttaat 
      ccttttggt atcgtcctg aaacagttt gcttcctca 
      gatcaggag gtgtcaagt gttggacga aatattttg 
      tcgtcaggt gacggccaa acgccgttt tcgtttggt 
      tcctcgttg atgctttgg ttcataaat ctgatggtt 
      ttgggtttt tgcttggcc aaaatgttt gtttacggc 
      gaccctgtt attccgagt agttcaaag gaggcgcag 
      aaattctgg cgccatcga agacaggcc gatatttgt 
      atcaaagcg ggtgataaa ctcgaagca aagcaggaa 
      cttcggagg tgtctccaa actttcggt ataacatta 
      gcaacgagt cgtttcgaa ctcgtgttg tcgctgtgc 
      tggcaagtg ttccgtcaa gttatgcac cggttatgg 
      atcgggcgg tggttgtct agacacgtc ggaggtttt 
      ttcgtcgac ggagccacg cgatacgaa gctctgact 
      tcctgccgc gaactttct ttggtttat cacgactta 
      catcagctc caattggtg gatgggccg gaagagact 
      tgtcatctc ctcggcttg acaactgca ttaagtgca 
      ctgaacttg gccgaagcg gcgaaaccc aaaatgaag 
      tcggttcaa atgatcgac atttacgtc gccttggct 
      ttgaggatt aaagcctcg tgtttgaat attttacaa 
      gggatccag agatattac ctaggtctg gcgaagtta 
      gcatcgacc aattcttgc gatccgatt ccgccgagg 
      ttgcagtgg ctgttgact cctcacggg ttcaagttt 
      ttcctttcg cagaggttt ctctacgat gccaaagcc 
      acgaatttg ccttttagt tgtattggc aacgtttcc 
      gatcccttg tccttcgct atgaagtcg tatagagaa 
      catttactg gaaactgca ctacaaact ttaacgact 
      cccggtaat aaaaatgac gtatatgaa gacaaaaag 
      acggacatt acggacgtg tcaacttac gtccatttg 
      ttgcttgaa aatgttgcc accgatgta cacacagtt 
      tttaattcg agtcaggcg cacagtaat tgtgacgaa 
      atagcccac tggtggact tcagtcgtc gatgtggcc 
      tctcattgg ctccttgga gaggagaac atcgatgat 
      gtgtataaa aaagttgaa aatatacca gaatcattg 
      ttacatctc gaaaatccg cttcctaaa gccgtgatt 
      ggcgccttt gtggctagg aaaaattat cttactgct 
      ggatcgagt gtttcgcat aagaaaatt ctgaagcag 
      tgtgatcac gccagtcag ttgttaacc gacagtctg 
      acctattcc agctgcaaa tcacaggat aatttgatt 
      ttgttgtgt caactgctg gtgtgcgac tggatcctt 
      gaaaccaga acgtccctt tgggaggac tcagttgag 
      gcatcaaca acgccgatc ccagtccct aacaatgtc 
      ctaacggcg ttccaaaaa gatttatct tcattgcga 
      accctagcc caaaatatt cccagcgcg ttgcctcgg 
      gtctttctg tacgaagct accgcccgc ttgatggcg 
      ggtgcagcc ccaggccgc actcagcag ctcctcgat 
      cgcagtttg cgacagcgt cacgctcgt tcgtcgatg 
      atttgcggc aaaggcgat aaaaaccaa caggatgtt 
      ggaggagag cgccaacac gcgacggct ttgtatatg 
      gcttgcaaa catttgccc ggtcaattg atgtcatcg 
      cctggagaa agggccgga atgttggtc gaagcagcg 
      aaaactatg gaacgaatc ggcgataaa aagaaactg 
      caggaatgc tataagctg atgaagaca ctcggtgcc 
      aactcggcc acgaattag
    mRNA sequence
    • >TC007163 RA
      tttttaaaa ataatgcat ctagcccca tctatacct 
      cagtcacca aactactta caattaaga actgataaa 
      cgctaaaaa atctgtaaa agtgtatgg tgatgttta 
      tgtgttttt gtgttacat ctgtggtta cagttgact 
      caaaaaacg caaaaaatg gatgagaac ataccccca 
      tttagtggg gattttaat ctaaatgag ttacaaggt 
      atagacgat atcctaaca aactgcgaa aacgaattg 
      atgaagagc aatgagctg ttcccagaa gatgcccta 
      ttttcccag cttgaccct ctcttaccc atggacgat 
      gtcagcttc gatttggga aatatcagc gaaactacg 
      tttctggac cctcagacc ctctcacct ctcaagcag 
      caaaatcct gtgttcaac tcccagaat tttcaatca 
      aatcagttt gcaaacgcc caaaaccgg caagctttc 
      acaacattg aacaagcca gacgtgcag cacgtccaa 
      aatccaact gctgttatc atttcccaa tcacagcct 
      cttgtttac tccagttta ccaatccag caaaacaat 
      cagcaacat ataatccta caaccaaat cccaaatcc 
      gaacaaatc acgaaaacg cagcctgtt attgtacaa 
      aacctaaac caaatccct tctgataaa atgcaacag 
      ctgttgctc caagcgaaa attgttaaa actaatccc 
      caacctact gtcatgtac gctactaca gccgcttca 
      cctcaacct tctttgcat actttggtg aatagcggc 
      actcagatt ttgaccact ggaatccct ttggtccta 
      gacactgag aataaagtt cccatcaat aggattcct 
      caagggact gggaaggag cccaaggtt aaagaggtg 
      aagaggagt gctcataat gctatcgag aggaagtac 
      aggaccagc attaatgat aagattgtc gaattgaaa 
      aatatcgtc gtggggact gaagcaaag ttaaacaaa 
      tccggaatt ttgaagaaa acaatcgaa tacatccgc 
      tttttacaa aactcaaac acacgtctg aaacaagaa 
      aacatggct ctgaaaatg gccgctcgt caaaacact 
      ctcaaagat ttgctaacg acgggcaaa aatatggaa 
      tatccccct caagacact cctccccat tcggacatt 
      tcgtcctca tcacccgac cactcctta ccatcaagt 
      cccgagtgt tcgacattt attaaggat gagagtgac 
      gaagagacc gttgggatt tccaaaggt cttttggac 
      caaacccga attacaatg ttcatgttt atgttggcg 
      gtcatttcc tttaatcct tttggtatc gtcctgaaa 
      cagtttgct tcctcagat caggaggtg tcaagtgtt 
      ggacgaaat attttgtcg tcaggtgac ggccaaacg 
      ccgttttcg tttggttcc tcgttgatg ctttggttc 
      ataaatctg atggttttg ggtttttgc ttggccaaa 
      atgtttgtt tacggcgac cctgttatt ccgagtagt 
      tcaaaggag gcgcagaaa ttctggcgc catcgaaga 
      caggccgat atttgtatc aaagcgggt gataaactc 
      gaagcaaag caggaactt cggaggtgt ctccaaact 
      ttcggtata acattagca acgagtcgt ttcgaactc 
      gtgttgtcg ctgtgctgg caagtgttc cgtcaagtt 
      atgcaccgg ttatggatc gggcggtgg ttgtctaga 
      cacgtcgga ggttttttc gtcgacgga gccacgcga 
      tacgaagct ctgacttcc tgccgcgaa ctttctttg 
      gtttatcac gacttacat cagctccaa ttggtggat 
      gggccggaa gagacttgt catctcctc ggcttgaca 
      actgcatta agtgcactg aacttggcc gaagcggcg 
      aaacccaaa atgaagtcg gttcaaatg atcgacatt 
      tacgtcgcc ttggctttg aggattaaa gcctcgtgt 
      ttgaatatt ttacaaggg atccagaga tattaccta 
      ggtctggcg aagttagca tcgaccaat tcttgcgat 
      ccgattccg ccgaggttg cagtggctg ttgactcct 
      cacgggttc aagtttttc ctttcgcag aggtttctc 
      tacgatgcc aaagccacg aatttgcct tttagttgt 
      attggcaac gtttccgat cccttgtcc ttcgctatg 
      aagtcgtat agagaacat ttactggaa actgcacta 
      caaacttta acgactccc ggtaataaa aatgacgta 
      tatgaagac aaaaagacg gacattacg gacgtgtca 
      acttacgtc catttgttg cttgaaaat gttgccacc 
      gatgtacac acagttttt aattcgagt caggcgcac 
      agtaattgt gacgaaata gcccactgg tggacttca 
      gtcgtcgat gtggcctct cattggctc cttggagag 
      gagaacatc gatgatgtg tataaaaaa gttgaaaat 
      ataccagaa tcattgtta catctcgaa aatccgctt 
      cctaaagcc gtgattggc gcctttgtg gctaggaaa 
      aattatctt actgctgga tcgagtgtt tcgcataag 
      aaaattctg aagcagtgt gatcacgcc agtcagttg 
      ttaaccgac agtctgacc tattccagc tgcaaatca 
      caggataat ttgattttg ttgtgtcaa ctgctggtg 
      tgcgactgg atccttgaa accagaacg tccctttgg 
      gaggactca gttgaggca tcaacaacg ccgatccca 
      gtccctaac aatgtccta acggcgttc caaaaagat 
      ttatcttca ttgcgaacc ctagcccaa aatattccc 
      agcgcgttg cctcgggtc tttctgtac gaagctacc 
      gcccgcttg atggcgggt gcagcccca ggccgcact 
      cagcagctc ctcgatcgc agtttgcga cagcgtcac 
      gctcgttcg tcgatgatt tgcggcaaa ggcgataaa 
      aaccaacag gatgttgga ggagagcgc caacacgcg 
      acggctttg tatatggct tgcaaacat ttgcccggt 
      caattgatg tcatcgcct ggagaaagg gccggaatg 
      ttggtcgaa gcagcgaaa actatggaa cgaatcggc 
      gataaaaag aaactgcag gaatgctat aagctgatg 
      aagacactc ggtgccaac tcggccacg aattagcgt 
      agttaattt ttatagcgg gcgtaaaac gtgatgttt 
      atgtcgggt tttgatttt aattatttg atgtgacaa 
      tatttattt tgtacaaat tctttttaa taaatagtg 
      tgtgagaag