| General information | |||
| TC007163 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 8312858..8320690 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007163 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01135 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
- >TC007163 RA
MDENIPPFS GDFNLNELQ GIDDILTNC ENELMKSNE LFPEDALFS QLDPLLPMD DVSFDLGNI SETTFLDPQ TLSPLKQQN PVFNSQNFQ SNQFANAQN RQAFTTLNK PDVQHVQNP TAVIISQSQ PLVYSSLPI QQNNQQHII LQPNPKSEQ ITKTQPVIV QNLNQIPSD KMQQLLLQA KIVKTNPQP TVMYATTAA SPQPSLHTL VNSGTQILT TGIPLVLDT ENKVPINRI PQGTGKEPK VKEVKRSAH NAIERKYRT SINDKIVEL KNIVVGTEA KLNKSGILK KTIEYIRFL QNSNTRLKQ ENMALKMAA RQNTLKDLL TTGKNMEYP PQDTPPHSD ISSSSPDHS LPSSPECST FIKDESDEE TVGISKGLL DQTRITMFM FMLAVISFN PFGIVLKQF ASSDQEVSS VGRNILSSG DGQTPFSFG SSLMLWFIN LMVLGFCLA KMFVYGDPV IPSSSKEAQ KFWRHRRQA DICIKAGDK LEAKQELRR CLQTFGITL ATSRFELVL SLCWQVFRQ VMHRLWIGR WLSRHVGGF FVDGATRYE ALTSCRELS LVYHDLHQL QLVDGPEET CHLLGLTTA LSALNLAEA AKPKMKSVQ MIDIYVALA LRIKASCLN ILQGIQRYY LGLAKLAST NSCDPIPPR LQWLLTPHG FKFFLSQRF LYDAKATNL PFSCIGNVS DPLSFAMKS YREHLLETA LQTLTTPGN KNDVYEDKK TDITDVSTY VHLLLENVA TDVHTVFNS SQAHSNCDE IAHWWTSVV DVASHWLLG EENIDDVYK KVENIPESL LHLENPLPK AVIGAFVAR KNYLTAGSS VSHKKILKQ CDHASQLLT DSLTYSSCK SQDNLILLC QLLVCDWIL ETRTSLWED SVEASTTPI PVPNNVLTA FQKDLSSLR TLAQNIPSA LPRVFLYEA TARLMAGAA PGRTQQLLD RSLRQRHAR SSMICGKGD KNQQDVGGE RQHATALYM ACKHLPGQL MSSPGERAG MLVEAAKTM ERIGDKKKL QECYKLMKT LGANSATN
- >TC007163 RA
atggatgag aacataccc ccatttagt ggggatttt aatctaaat gagttacaa ggtatagac gatatccta acaaactgc gaaaacgaa ttgatgaag agcaatgag ctgttccca gaagatgcc ctattttcc cagcttgac cctctctta cccatggac gatgtcagc ttcgatttg ggaaatatc agcgaaact acgtttctg gaccctcag accctctca cctctcaag cagcaaaat cctgtgttc aactcccag aattttcaa tcaaatcag tttgcaaac gcccaaaac cggcaagct ttcacaaca ttgaacaag ccagacgtg cagcacgtc caaaatcca actgctgtt atcatttcc caatcacag cctcttgtt tactccagt ttaccaatc cagcaaaac aatcagcaa catataatc ctacaacca aatcccaaa tccgaacaa atcacgaaa acgcagcct gttattgta caaaaccta aaccaaatc ccttctgat aaaatgcaa cagctgttg ctccaagcg aaaattgtt aaaactaat ccccaacct actgtcatg tacgctact acagccgct tcacctcaa ccttctttg catactttg gtgaatagc ggcactcag attttgacc actggaatc cctttggtc ctagacact gagaataaa gttcccatc aataggatt cctcaaggg actgggaag gagcccaag gttaaagag gtgaagagg agtgctcat aatgctatc gagaggaag tacaggacc agcattaat gataagatt gtcgaattg aaaaatatc gtcgtgggg actgaagca aagttaaac aaatccgga attttgaag aaaacaatc gaatacatc cgcttttta caaaactca aacacacgt ctgaaacaa gaaaacatg gctctgaaa atggccgct cgtcaaaac actctcaaa gatttgcta acgacgggc aaaaatatg gaatatccc cctcaagac actcctccc cattcggac atttcgtcc tcatcaccc gaccactcc ttaccatca agtcccgag tgttcgaca tttattaag gatgagagt gacgaagag accgttggg atttccaaa ggtcttttg gaccaaacc cgaattaca atgttcatg tttatgttg gcggtcatt tcctttaat ccttttggt atcgtcctg aaacagttt gcttcctca gatcaggag gtgtcaagt gttggacga aatattttg tcgtcaggt gacggccaa acgccgttt tcgtttggt tcctcgttg atgctttgg ttcataaat ctgatggtt ttgggtttt tgcttggcc aaaatgttt gtttacggc gaccctgtt attccgagt agttcaaag gaggcgcag aaattctgg cgccatcga agacaggcc gatatttgt atcaaagcg ggtgataaa ctcgaagca aagcaggaa cttcggagg tgtctccaa actttcggt ataacatta gcaacgagt cgtttcgaa ctcgtgttg tcgctgtgc tggcaagtg ttccgtcaa gttatgcac cggttatgg atcgggcgg tggttgtct agacacgtc ggaggtttt ttcgtcgac ggagccacg cgatacgaa gctctgact tcctgccgc gaactttct ttggtttat cacgactta catcagctc caattggtg gatgggccg gaagagact tgtcatctc ctcggcttg acaactgca ttaagtgca ctgaacttg gccgaagcg gcgaaaccc aaaatgaag tcggttcaa atgatcgac atttacgtc gccttggct ttgaggatt aaagcctcg tgtttgaat attttacaa gggatccag agatattac ctaggtctg gcgaagtta gcatcgacc aattcttgc gatccgatt ccgccgagg ttgcagtgg ctgttgact cctcacggg ttcaagttt ttcctttcg cagaggttt ctctacgat gccaaagcc acgaatttg ccttttagt tgtattggc aacgtttcc gatcccttg tccttcgct atgaagtcg tatagagaa catttactg gaaactgca ctacaaact ttaacgact cccggtaat aaaaatgac gtatatgaa gacaaaaag acggacatt acggacgtg tcaacttac gtccatttg ttgcttgaa aatgttgcc accgatgta cacacagtt tttaattcg agtcaggcg cacagtaat tgtgacgaa atagcccac tggtggact tcagtcgtc gatgtggcc tctcattgg ctccttgga gaggagaac atcgatgat gtgtataaa aaagttgaa aatatacca gaatcattg ttacatctc gaaaatccg cttcctaaa gccgtgatt ggcgccttt gtggctagg aaaaattat cttactgct ggatcgagt gtttcgcat aagaaaatt ctgaagcag tgtgatcac gccagtcag ttgttaacc gacagtctg acctattcc agctgcaaa tcacaggat aatttgatt ttgttgtgt caactgctg gtgtgcgac tggatcctt gaaaccaga acgtccctt tgggaggac tcagttgag gcatcaaca acgccgatc ccagtccct aacaatgtc ctaacggcg ttccaaaaa gatttatct tcattgcga accctagcc caaaatatt cccagcgcg ttgcctcgg gtctttctg tacgaagct accgcccgc ttgatggcg ggtgcagcc ccaggccgc actcagcag ctcctcgat cgcagtttg cgacagcgt cacgctcgt tcgtcgatg atttgcggc aaaggcgat aaaaaccaa caggatgtt ggaggagag cgccaacac gcgacggct ttgtatatg gcttgcaaa catttgccc ggtcaattg atgtcatcg cctggagaa agggccgga atgttggtc gaagcagcg aaaactatg gaacgaatc ggcgataaa aagaaactg caggaatgc tataagctg atgaagaca ctcggtgcc aactcggcc acgaattag
- >TC007163 RA
tttttaaaa ataatgcat ctagcccca tctatacct cagtcacca aactactta caattaaga actgataaa cgctaaaaa atctgtaaa agtgtatgg tgatgttta tgtgttttt gtgttacat ctgtggtta cagttgact caaaaaacg caaaaaatg gatgagaac ataccccca tttagtggg gattttaat ctaaatgag ttacaaggt atagacgat atcctaaca aactgcgaa aacgaattg atgaagagc aatgagctg ttcccagaa gatgcccta ttttcccag cttgaccct ctcttaccc atggacgat gtcagcttc gatttggga aatatcagc gaaactacg tttctggac cctcagacc ctctcacct ctcaagcag caaaatcct gtgttcaac tcccagaat tttcaatca aatcagttt gcaaacgcc caaaaccgg caagctttc acaacattg aacaagcca gacgtgcag cacgtccaa aatccaact gctgttatc atttcccaa tcacagcct cttgtttac tccagttta ccaatccag caaaacaat cagcaacat ataatccta caaccaaat cccaaatcc gaacaaatc acgaaaacg cagcctgtt attgtacaa aacctaaac caaatccct tctgataaa atgcaacag ctgttgctc caagcgaaa attgttaaa actaatccc caacctact gtcatgtac gctactaca gccgcttca cctcaacct tctttgcat actttggtg aatagcggc actcagatt ttgaccact ggaatccct ttggtccta gacactgag aataaagtt cccatcaat aggattcct caagggact gggaaggag cccaaggtt aaagaggtg aagaggagt gctcataat gctatcgag aggaagtac aggaccagc attaatgat aagattgtc gaattgaaa aatatcgtc gtggggact gaagcaaag ttaaacaaa tccggaatt ttgaagaaa acaatcgaa tacatccgc tttttacaa aactcaaac acacgtctg aaacaagaa aacatggct ctgaaaatg gccgctcgt caaaacact ctcaaagat ttgctaacg acgggcaaa aatatggaa tatccccct caagacact cctccccat tcggacatt tcgtcctca tcacccgac cactcctta ccatcaagt cccgagtgt tcgacattt attaaggat gagagtgac gaagagacc gttgggatt tccaaaggt cttttggac caaacccga attacaatg ttcatgttt atgttggcg gtcatttcc tttaatcct tttggtatc gtcctgaaa cagtttgct tcctcagat caggaggtg tcaagtgtt ggacgaaat attttgtcg tcaggtgac ggccaaacg ccgttttcg tttggttcc tcgttgatg ctttggttc ataaatctg atggttttg ggtttttgc ttggccaaa atgtttgtt tacggcgac cctgttatt ccgagtagt tcaaaggag gcgcagaaa ttctggcgc catcgaaga caggccgat atttgtatc aaagcgggt gataaactc gaagcaaag caggaactt cggaggtgt ctccaaact ttcggtata acattagca acgagtcgt ttcgaactc gtgttgtcg ctgtgctgg caagtgttc cgtcaagtt atgcaccgg ttatggatc gggcggtgg ttgtctaga cacgtcgga ggttttttc gtcgacgga gccacgcga tacgaagct ctgacttcc tgccgcgaa ctttctttg gtttatcac gacttacat cagctccaa ttggtggat gggccggaa gagacttgt catctcctc ggcttgaca actgcatta agtgcactg aacttggcc gaagcggcg aaacccaaa atgaagtcg gttcaaatg atcgacatt tacgtcgcc ttggctttg aggattaaa gcctcgtgt ttgaatatt ttacaaggg atccagaga tattaccta ggtctggcg aagttagca tcgaccaat tcttgcgat ccgattccg ccgaggttg cagtggctg ttgactcct cacgggttc aagtttttc ctttcgcag aggtttctc tacgatgcc aaagccacg aatttgcct tttagttgt attggcaac gtttccgat cccttgtcc ttcgctatg aagtcgtat agagaacat ttactggaa actgcacta caaacttta acgactccc ggtaataaa aatgacgta tatgaagac aaaaagacg gacattacg gacgtgtca acttacgtc catttgttg cttgaaaat gttgccacc gatgtacac acagttttt aattcgagt caggcgcac agtaattgt gacgaaata gcccactgg tggacttca gtcgtcgat gtggcctct cattggctc cttggagag gagaacatc gatgatgtg tataaaaaa gttgaaaat ataccagaa tcattgtta catctcgaa aatccgctt cctaaagcc gtgattggc gcctttgtg gctaggaaa aattatctt actgctgga tcgagtgtt tcgcataag aaaattctg aagcagtgt gatcacgcc agtcagttg ttaaccgac agtctgacc tattccagc tgcaaatca caggataat ttgattttg ttgtgtcaa ctgctggtg tgcgactgg atccttgaa accagaacg tccctttgg gaggactca gttgaggca tcaacaacg ccgatccca gtccctaac aatgtccta acggcgttc caaaaagat ttatcttca ttgcgaacc ctagcccaa aatattccc agcgcgttg cctcgggtc tttctgtac gaagctacc gcccgcttg atggcgggt gcagcccca ggccgcact cagcagctc ctcgatcgc agtttgcga cagcgtcac gctcgttcg tcgatgatt tgcggcaaa ggcgataaa aaccaacag gatgttgga ggagagcgc caacacgcg acggctttg tatatggct tgcaaacat ttgcccggt caattgatg tcatcgcct ggagaaagg gccggaatg ttggtcgaa gcagcgaaa actatggaa cgaatcggc gataaaaag aaactgcag gaatgctat aagctgatg aagacactc ggtgccaac tcggccacg aattagcgt agttaattt ttatagcgg gcgtaaaac gtgatgttt atgtcgggt tttgatttt aattatttg atgtgacaa tatttattt tgtacaaat tctttttaa taaatagtg tgtgagaag
