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Gene information
General information
TC007155 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8411162..8413310
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007155
    iBeetle screen iB_01133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, entire repetition development delayed, hours 72
    Pupal morphology
    remarks: later than schedule, entire repetition development delayed, hours 72
    Adult morphology
    remarks: later than schedule, entire repetition development delayed, hours 72
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007155 RA
      MANILRLVV VVCGFCSSD VAFAGFTTR KLGPQPIWN 
      STVTDKLRQ DLLLNYDKF ARPAQHYNT TKVVFGLTI 
      RHIELNEFK STLVVHSWI RLSWKDEKL QWNSTNYGG 
      LETLNLADH EIWQPDIFL YNSATSSGI THYGNVNCI 
      VYEGGDVLW VPPAQFSVL CNLNLKYWP FDTQRCEMI 
      FGSWTYNGD QIDIDPDND GVKMELLIE NSEWNIVDK 
      SLKKNMKYY ACCPSPYPD ITIDLTLSR ISPSYKALI 
      VTPAFVVII LVLVNFWLP PQAGEKLIL NGCTALIIC 
      LFMLYFTQK IPTMGTHTP LIVFFYSSC LYVVGFSII 
      TSVVVIWLS RTKHSSPLP WIVKQPLTG TFGKILGLQ 
      TYIQQSSMT SHRVTAEEM RDHQVTDFD ENNSGDEHH 
      IIKSSNKMS LQQDWILLA AAIDRITFC FFSFLFLIL 
      TIVYSV
    CDS sequence
    • >TC007155 RA
      atggcaaac atactgagg ctcgtggtt gtggtgtgc 
      ggcttttgc agctcggat gtagctttc gcaggattc 
      accaccaga aaactgggg ccccagccc atttggaat 
      tccacagtc actgataag ttaagacaa gacctcctg 
      cttaattat gataaattc gcaaggcca gcgcaacac 
      tacaacacg acgaaagtc gtctttggt ttaacaata 
      cgccacatc gaacttaac gaattcaaa tcgacactt 
      gtggtccat tcgtggata cgtctgagc tggaaagat 
      gagaaactg caatggaat tcaacaaat tacggcggg 
      cttgagacg ctcaacttg gccgatcac gaaatttgg 
      caaccggac atatttttg tacaacagt gcgacaagt 
      tctggcatt acccactac ggtaatgtg aattgcata 
      gtttacgaa ggtggggac gtgctctgg gtgcccccg 
      gcacaattc agtgttttg tgtaatttg aatttgaaa 
      tattggcct tttgatacg caacggtgc gaaatgatt 
      tttgggtcc tggacctac aatggggac caaattgat 
      atcgaccct gataacgac ggagtcaag atggaactt 
      ctgatcgaa aactcggag tggaatata gtcgacaag 
      tccttgaag aagaacatg aaatattac gcctgttgt 
      ccgagccct tatccagat atcacgatc gatctcacc 
      ctcagccga atttcaccc tcctacaaa gcccttatt 
      gttacacca gctttcgtt gtgataatt ttagtgtta 
      gttaatttt tggctgcca ccccaagca ggcgaaaaa 
      ctcatcctg aacggttgt acggctttg attatttgt 
      ttatttatg ttgtatttc acgcagaag atacctact 
      atgggcacg cacacgcct ctgattgtt tttttctac 
      agtagttgt ttgtacgta gtcgggttt tcgataatt 
      acctcagtg gtggtaatt tggttatcg aggactaag 
      cacagttcg cccctgcct tggattgtt aagcaaccc 
      ctgactgga acatttggt aaaattctg gggctacaa 
      acttacatc caacagtcg tcaatgaca tcgcatagg 
      gtcacagct gaggaaatg cgagaccac caggttaca 
      gatttcgac gagaataac agtggagac gagcaccac 
      ataatcaaa tcgtccaat aaaatgtcc ctacagcag 
      gactggatt ttactagca gcggccata gatcggatc 
      actttttgc tttttctct ttcctattc ctcatttta 
      accatagtt tattccgtg tga
    mRNA sequence
    • >TC007155 RA
      tatgattta agcccctag aatggcaaa catactgag 
      gctcgtggt tgtggtgtg cggcttttg cagctcgga 
      tgtagcttt cgcaggatt caccaccag aaaactggg 
      gccccagcc catttggaa ttccacagt cactgataa 
      gttaagaca agacctcct gcttaatta tgataaatt 
      cgcaaggcc agcgcaaca ctacaacac gacgaaagt 
      cgtctttgg tttaacaat acgccacat cgaacttaa 
      cgaattcaa atcgacact tgtggtcca ttcgtggat 
      acgtctgag ctggaaaga tgagaaact gcaatggaa 
      ttcaacaaa ttacggcgg gcttgagac gctcaactt 
      ggccgatca cgaaatttg gcaaccgga catattttt 
      gtacaacag tgcgacaag ttctggcat tacccacta 
      cggtaatgt gaattgcat agtttacga aggtgggga 
      cgtgctctg ggtgccccc ggcacaatt cagtgtttt 
      gtgtaattt gaatttgaa atattggcc ttttgatac 
      gcaacggtg cgaaatgat ttttgggtc ctggaccta 
      caatgggga ccaaattga tatcgaccc tgataacga 
      cggagtcaa gatggaact tctgatcga aaactcgga 
      gtggaatat agtcgacaa gtccttgaa gaagaacat 
      gaaatatta cgcctgttg tccgagccc ttatccaga 
      tatcacgat cgatctcac cctcagccg aatttcacc 
      ctcctacaa agcccttat tgttacacc agctttcgt 
      tgtgataat tttagtgtt agttaattt ttggctgcc 
      accccaagc aggcgaaaa actcatcct gaacggttg 
      tacggcttt gattatttg tttatttat gttgtattt 
      cacgcagaa gatacctac tatgggcac gcacacgcc 
      tctgattgt ttttttcta cagtagttg tttgtacgt 
      agtcgggtt ttcgataat tacctcagt ggtggtaat 
      ttggttatc gaggactaa gcacagttc gcccctgcc 
      ttggattgt taagcaacc cctgactgg aacatttgg 
      taaaattct ggggctaca aacttacat ccaacagtc 
      gtcaatgac atcgcatag ggtcacagc tgaggaaat 
      gcgagacca ccaggttac agatttcga cgagaataa 
      cagtggaga cgagcacca cataatcaa atcgtccaa 
      taaaatgtc cctacagca ggactggat tttactagc 
      agcggccat agatcggat cactttttg ctttttctc 
      tttcctatt cctcatttt aaccatagt ttattccgt 
      gtgaaataa aaaacgctt cgtgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY