| General information | |||
| TC007136 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 8861172..8865147 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007136 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01130 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC007136 RA
MPFKPAENP KCPKCQKSV YAAEERVAG GYKWHKSCF KCGLCGKML DSTNCTEHE AELYCKNCH ARKYGPKGY GFGGGAGCL SMDTGSHLQ QGS
- >TC007136 RB
MPFKPAENP KCPKCQKSV YAAEERVAG GYKWHKSCF KCGLCGKML DSTNCTEHE AELYCKNCH ARKYGPKGY GFGGGAGCL SMDTGSHLQ QGDDIVVSR APLLPKAIA KAPEGEGCP RCGGYVYAA EQMLARGRA FHKTCFKCA VCNKGLDSV NVSEGPDKD IYCKVCYGK KFGPKGYGY GQGGGTLQS DCYAVSDAA PKTTVIDTA CIKAPPGQG CPRCGGVVF AAEEVLAKG RPWHRKCFK CKDCTKTLD SIIACDGPD SDVYCKTCY GKKWGPHGY GFACGSGFL QTDGLTEDE ISQTRPFYN PDTTSIKAP PGQGCPRCG GMVFAAEQQ LAKGTMWHK KCFNCAECH RPLDSVLAC DGPDREIHC RACYSKLFG PKGFGFGHT PTLVCADGA PAAIHNDIR PNSGPKAAP GQGCRRCGY AVYAAEQMI SKNGIWHKR CFCCVECKR SLDSTNQND APDGEIYCR GCYGRNFGP KGVGFGMGA GTLTMA
- >TC007136 RC
MAKGNLLFS LLTYDDIVV SRAPLLPKA IAKAPEGEG CPRCGGYVY AAEQMLARG RAFHKTCFK CAVCNKGLD SVNVSEGPD KDIYCKVCY GKKFGPKGY GYGQGGGTL QSDCYAVSD AAPKTTVID TACIKAPPG QGCPRCGGV VFAAEEVLA KGRPWHRKC FKCKDCTKT LDSIIACDG PDSDVYCKT CYGKKWGPH GYGFACGSG FLQTDGLTE DEISQTRPF YNPDTTSIK APPGQGCPR CGGMVFAAE QQLAKGTMW HKKCFNCAE CHRPLDSVL ACDGPDREI HCRACYSKL FGPKGFGFG HTPTLVCAD GAPAAIHND IRPNSGPKA APGQGCRRC GYAVYAAEQ MISKNGIWH KRCFCCVEC KRSLDSTNQ NDAPDGEIY CRGCYGRNF GPKGVGFGM GAGTLTMA
- >TC007136 RA
atgcctttc aagcctgcc gaaaacccc aaatgcccc aagtgccag aaatccgtc tacgccgct gaagagcgt gtagctggc ggttacaaa tggcacaag tcctgcttc aaatgcggc ctttgcggc aaaatgttg gactccacc aactgcaca gaacacgaa gccgaattg tattgcaag aactgccac gcccgcaaa tacggacct aaaggctac ggctttggc ggtggtgcc ggttgcctt agcatggac actggctcc catctccaa caaggctcc taa
- >TC007136 RB
atgcctttc aagcctgcc gaaaacccc aaatgcccc aagtgccag aaatccgtc tacgccgct gaagagcgt gtagctggc ggttacaaa tggcacaag tcctgcttc aaatgcggc ctttgcggc aaaatgttg gactccacc aactgcaca gaacacgaa gccgaattg tattgcaag aactgccac gcccgcaaa tacggacct aaaggctac ggctttggc ggtggtgcc ggttgcctt agcatggac actggctcc catctccaa caaggcgac gatattgtt gtttctcga gcccccctt ttgccgaaa gccatcgcg aaggccccc gaaggcgaa ggatgtccc cgatgcggc ggttacgtg tacgctgcc gaacagatg ttagcaaga ggaagagcg ttccacaaa acctgtttc aaatgtgcc gtgtgtaac aaaggattg gattctgtt aatgtctct gaaggtccc gacaaggat atttattgc aaagtttgt tacggaaag aaatttgga ccgaaagga tatggttac ggccaaggt ggtggcacc cttcaaagc gattgttac gccgttagt gatgccgcc cccaaaacc acggtgatt gacaccgct tgcatcaag gctcctcca ggccaaggt tgtccccgt tgtggtggt gtagtgttt gccgccgaa gaggtcctc gcgaaagga agaccttgg catcgtaaa tgtttcaaa tgcaaggat tgtactaag accctcgac tcaattatc gcttgcgat ggacctgat agcgatgtc tattgtaag acctgttac ggcaaaaag tggggccct cacggatac ggcttcgct tgtggctca ggttttctc cagactgat gggctaacc gaagatgaa atttcgcaa acacgacct ttctataat cccgatacg acctctatc aaggcccct ccaggacaa ggatgcccc cgatgtgga ggaatggtc ttcgctgcc gagcaacaa ctggccaaa ggaacaatg tggcacaag aagtgcttc aactgcgcc gagtgtcat cgtcctctc gattcagtc ctggcttgt gacggtcct gaccgtgaa atccactgc agggcctgc tacagcaag ctgtttggt cccaaaggg ttcggtttt ggccacact cccactctg gtgtgtgcc gacggcgcc cctgccgcc atccacaac gacatcaga cccaacagc gggccaaaa gccgcccct gggcaaggc tgccgtcgt tgtggttat gccgtctat gctgctgag cagatgatc agcaaaaac ggcatctgg cacaagagg tgcttctgc tgtgttgaa tgcaagcgc tcgttggat tccaccaac caaaacgat gcccccgat ggcgaaatt tactgccga ggttgctac ggaaggaat ttcgggccc aagggagtt ggttttgga atgggcgct ggcactctg actatggct tag
- >TC007136 RC
atggctaaa ggcaatctg ctcttctcg ctattaaca tacgacgat attgttgtt tctcgagcc ccccttttg ccgaaagcc atcgcgaag gcccccgaa ggcgaagga tgtccccga tgcggcggt tacgtgtac gctgccgaa cagatgtta gcaagagga agagcgttc cacaaaacc tgtttcaaa tgtgccgtg tgtaacaaa ggattggat tctgttaat gtctctgaa ggtcccgac aaggatatt tattgcaaa gtttgttac ggaaagaaa tttggaccg aaaggatat ggttacggc caaggtggt ggcaccctt caaagcgat tgttacgcc gttagtgat gccgccccc aaaaccacg gtgattgac accgcttgc atcaaggct cctccaggc caaggttgt ccccgttgt ggtggtgta gtgtttgcc gccgaagag gtcctcgcg aaaggaaga ccttggcat cgtaaatgt ttcaaatgc aaggattgt actaagacc ctcgactca attatcgct tgcgatgga cctgatagc gatgtctat tgtaagacc tgttacggc aaaaagtgg ggccctcac ggatacggc ttcgcttgt ggctcaggt tttctccag actgatggg ctaaccgaa gatgaaatt tcgcaaaca cgacctttc tataatccc gatacgacc tctatcaag gcccctcca ggacaagga tgcccccga tgtggagga atggtcttc gctgccgag caacaactg gccaaagga acaatgtgg cacaagaag tgcttcaac tgcgccgag tgtcatcgt cctctcgat tcagtcctg gcttgtgac ggtcctgac cgtgaaatc cactgcagg gcctgctac agcaagctg tttggtccc aaagggttc ggttttggc cacactccc actctggtg tgtgccgac ggcgcccct gccgccatc cacaacgac atcagaccc aacagcggg ccaaaagcc gcccctggg caaggctgc cgtcgttgt ggttatgcc gtctatgct gctgagcag atgatcagc aaaaacggc atctggcac aagaggtgc ttctgctgt gttgaatgc aagcgctcg ttggattcc accaaccaa aacgatgcc cccgatggc gaaatttac tgccgaggt tgctacgga aggaatttc gggcccaag ggagttggt tttggaatg ggcgctggc actctgact atggcttag
- >TC007136 RA
ttcgaattt cagtggttc tagtgtaag gacaccatt gaggacctc tccaccgac tactgtacg attccgacc aggtcgact agatcaaga tgcctttca agcctgccg aaaacccca aatgcccca agtgccaga aatccgtct acgccgctg aagagcgtg tagctggcg gttacaaat ggcacaagt cctgcttca aatgcggcc tttgcggca aaatgttgg actccacca actgcacag aacacgaag ccgaattgt attgcaaga actgccacg cccgcaaat acggaccta aaggctacg gctttggcg gtggtgccg gttgcctta gcatggaca ctggctccc atctccaac aaggctcct aaggaatca gccggggac agaagagcg gccttgtca ccaacaaca ccatcgccg cctcacaaa acctactcc ttccatccc acgtataat taattatat gttgtgtta tatttatag attttacaa taacaaata ggttactgt ttataaaat ttgttttac atggtacaa acaattcga ttattattt tgtaataat tgaatcgta ataaatcac gtcaaaaat t
- >TC007136 RB
ttcgaattt cagtggttc tagtgtaag gacaccatt gaggacctc tccaccgac tactgtacg attccgacc aggtcgact agatcaaga tgcctttca agcctgccg aaaacccca aatgcccca agtgccaga aatccgtct acgccgctg aagagcgtg tagctggcg gttacaaat ggcacaagt cctgcttca aatgcggcc tttgcggca aaatgttgg actccacca actgcacag aacacgaag ccgaattgt attgcaaga actgccacg cccgcaaat acggaccta aaggctacg gctttggcg gtggtgccg gttgcctta gcatggaca ctggctccc atctccaac aaggcgacg atattgttg tttctcgag ccccccttt tgccgaaag ccatcgcga aggcccccg aaggcgaag gatgtcccc gatgcggcg gttacgtgt acgctgccg aacagatgt tagcaagag gaagagcgt tccacaaaa cctgtttca aatgtgccg tgtgtaaca aaggattgg attctgtta atgtctctg aaggtcccg acaaggata tttattgca aagtttgtt acggaaaga aatttggac cgaaaggat atggttacg gccaaggtg gtggcaccc ttcaaagcg attgttacg ccgttagtg atgccgccc ccaaaacca cggtgattg acaccgctt gcatcaagg ctcctccag gccaaggtt gtccccgtt gtggtggtg tagtgtttg ccgccgaag aggtcctcg cgaaaggaa gaccttggc atcgtaaat gtttcaaat gcaaggatt gtactaaga ccctcgact caattatcg cttgcgatg gacctgata gcgatgtct attgtaaga cctgttacg gcaaaaagt ggggccctc acggatacg gcttcgctt gtggctcag gttttctcc agactgatg ggctaaccg aagatgaaa tttcgcaaa cacgacctt tctataatc ccgatacga cctctatca aggcccctc caggacaag gatgccccc gatgtggag gaatggtct tcgctgccg agcaacaac tggccaaag gaacaatgt ggcacaaga agtgcttca actgcgccg agtgtcatc gtcctctcg attcagtcc tggcttgtg acggtcctg accgtgaaa tccactgca gggcctgct acagcaagc tgtttggtc ccaaagggt tcggttttg gccacactc ccactctgg tgtgtgccg acggcgccc ctgccgcca tccacaacg acatcagac ccaacagcg ggccaaaag ccgcccctg ggcaaggct gccgtcgtt gtggttatg ccgtctatg ctgctgagc agatgatca gcaaaaacg gcatctggc acaagaggt gcttctgct gtgttgaat gcaagcgct cgttggatt ccaccaacc aaaacgatg cccccgatg gcgaaattt actgccgag gttgctacg gaaggaatt tcgggccca agggagttg gttttggaa tgggcgctg gcactctga ctatggctt agattcacc gacccgacc ctttgaact ctcttaacc gatgcggat tggttagga ctgttccac cttttagct tgtggtttt agcaggaat aatattatt actaaaaat gatgcttga ggacgcata ttttttttg gagattttt tggaattcc atttttgat agaggacac gacagaaag ggaacaaga ctcgaacgg cttattccg ttaccagtt ggtataaga ttatgaaag acttcctcg aacgaccag ggttttcct gaggttttt tgtacatat ctacactgg ccacaatgt tggtctaat atagcttcg cgctttgct actgtactc gatttgttt ttatgctat ttacaacgt aagtctttt tgtctagat gtactttca ttgtatatt atgtttaat aaaaagcta ataaagtta cctaaaaaa c
- >TC007136 RC
agaacggtg agacaatag atgatttaa gcatggcta aaggcaatc tgctcttct cgctattaa catacgacg atattgttg tttctcgag ccccccttt tgccgaaag ccatcgcga aggcccccg aaggcgaag gatgtcccc gatgcggcg gttacgtgt acgctgccg aacagatgt tagcaagag gaagagcgt tccacaaaa cctgtttca aatgtgccg tgtgtaaca aaggattgg attctgtta atgtctctg aaggtcccg acaaggata tttattgca aagtttgtt acggaaaga aatttggac cgaaaggat atggttacg gccaaggtg gtggcaccc ttcaaagcg attgttacg ccgttagtg atgccgccc ccaaaacca cggtgattg acaccgctt gcatcaagg ctcctccag gccaaggtt gtccccgtt gtggtggtg tagtgtttg ccgccgaag aggtcctcg cgaaaggaa gaccttggc atcgtaaat gtttcaaat gcaaggatt gtactaaga ccctcgact caattatcg cttgcgatg gacctgata gcgatgtct attgtaaga cctgttacg gcaaaaagt ggggccctc acggatacg gcttcgctt gtggctcag gttttctcc agactgatg ggctaaccg aagatgaaa tttcgcaaa cacgacctt tctataatc ccgatacga cctctatca aggcccctc caggacaag gatgccccc gatgtggag gaatggtct tcgctgccg agcaacaac tggccaaag gaacaatgt ggcacaaga agtgcttca actgcgccg agtgtcatc gtcctctcg attcagtcc tggcttgtg acggtcctg accgtgaaa tccactgca gggcctgct acagcaagc tgtttggtc ccaaagggt tcggttttg gccacactc ccactctgg tgtgtgccg acggcgccc ctgccgcca tccacaacg acatcagac ccaacagcg ggccaaaag ccgcccctg ggcaaggct gccgtcgtt gtggttatg ccgtctatg ctgctgagc agatgatca gcaaaaacg gcatctggc acaagaggt gcttctgct gtgttgaat gcaagcgct cgttggatt ccaccaacc aaaacgatg cccccgatg gcgaaattt actgccgag gttgctacg gaaggaatt tcgggccca agggagttg gttttggaa tgggcgctg gcactctga ctatggctt agattcacc gacccgacc ctttgaact ctcttaacc gatgcggat tggttagga ctgttccac cttttagct tgtggtttt agcaggaat aatattatt actaaaaat gatgcttga ggacgcata ttttttttg gagattttt tggaattcc atttttgat agaggacac gacagaaag ggaacaaga ctcgaacgg cttattccg ttaccagtt ggtataaga ttatgaaag acttcctcg aacgaccag ggttttcct gaggttttt tgtacatat ctacactgg ccacaatgt tggtctaat atagcttcg cgctttgct actgtactc gatttgttt ttatgctat ttacaacgt aagtctttt tgtctagat gtactttca ttgtatatt atgtttaat aaaaagcta ataaagtta cctaaaaaa c
