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Gene information
General information
TC007136 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 8861172..8865147
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007136
    iBeetle screen iB_01130
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, entire repetition development delayed, hours 72
    Pupal morphology
    remarks: later than schedule, entire repetition development delayed, hours 72
    Adult morphology
    remarks: later than schedule, entire repetition development delayed, hours 72
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007136 RA
      MPFKPAENP KCPKCQKSV YAAEERVAG 
      GYKWHKSCF KCGLCGKML DSTNCTEHE 
      AELYCKNCH ARKYGPKGY GFGGGAGCL 
      SMDTGSHLQ QGS
    • >TC007136 RB
      MPFKPAENP KCPKCQKSV YAAEERVAG GYKWHKSCF 
      KCGLCGKML DSTNCTEHE AELYCKNCH ARKYGPKGY 
      GFGGGAGCL SMDTGSHLQ QGDDIVVSR APLLPKAIA 
      KAPEGEGCP RCGGYVYAA EQMLARGRA FHKTCFKCA 
      VCNKGLDSV NVSEGPDKD IYCKVCYGK KFGPKGYGY 
      GQGGGTLQS DCYAVSDAA PKTTVIDTA CIKAPPGQG 
      CPRCGGVVF AAEEVLAKG RPWHRKCFK CKDCTKTLD 
      SIIACDGPD SDVYCKTCY GKKWGPHGY GFACGSGFL 
      QTDGLTEDE ISQTRPFYN PDTTSIKAP PGQGCPRCG 
      GMVFAAEQQ LAKGTMWHK KCFNCAECH RPLDSVLAC 
      DGPDREIHC RACYSKLFG PKGFGFGHT PTLVCADGA 
      PAAIHNDIR PNSGPKAAP GQGCRRCGY AVYAAEQMI 
      SKNGIWHKR CFCCVECKR SLDSTNQND APDGEIYCR 
      GCYGRNFGP KGVGFGMGA GTLTMA
    • >TC007136 RC
      MAKGNLLFS LLTYDDIVV SRAPLLPKA IAKAPEGEG 
      CPRCGGYVY AAEQMLARG RAFHKTCFK CAVCNKGLD 
      SVNVSEGPD KDIYCKVCY GKKFGPKGY GYGQGGGTL 
      QSDCYAVSD AAPKTTVID TACIKAPPG QGCPRCGGV 
      VFAAEEVLA KGRPWHRKC FKCKDCTKT LDSIIACDG 
      PDSDVYCKT CYGKKWGPH GYGFACGSG FLQTDGLTE 
      DEISQTRPF YNPDTTSIK APPGQGCPR CGGMVFAAE 
      QQLAKGTMW HKKCFNCAE CHRPLDSVL ACDGPDREI 
      HCRACYSKL FGPKGFGFG HTPTLVCAD GAPAAIHND 
      IRPNSGPKA APGQGCRRC GYAVYAAEQ MISKNGIWH 
      KRCFCCVEC KRSLDSTNQ NDAPDGEIY CRGCYGRNF 
      GPKGVGFGM GAGTLTMA
    CDS sequence
    • >TC007136 RA
      atgcctttc aagcctgcc gaaaacccc aaatgcccc 
      aagtgccag aaatccgtc tacgccgct gaagagcgt 
      gtagctggc ggttacaaa tggcacaag tcctgcttc 
      aaatgcggc ctttgcggc aaaatgttg gactccacc 
      aactgcaca gaacacgaa gccgaattg tattgcaag 
      aactgccac gcccgcaaa tacggacct aaaggctac 
      ggctttggc ggtggtgcc ggttgcctt agcatggac 
      actggctcc catctccaa caaggctcc taa
    • >TC007136 RB
      atgcctttc aagcctgcc gaaaacccc aaatgcccc 
      aagtgccag aaatccgtc tacgccgct gaagagcgt 
      gtagctggc ggttacaaa tggcacaag tcctgcttc 
      aaatgcggc ctttgcggc aaaatgttg gactccacc 
      aactgcaca gaacacgaa gccgaattg tattgcaag 
      aactgccac gcccgcaaa tacggacct aaaggctac 
      ggctttggc ggtggtgcc ggttgcctt agcatggac 
      actggctcc catctccaa caaggcgac gatattgtt 
      gtttctcga gcccccctt ttgccgaaa gccatcgcg 
      aaggccccc gaaggcgaa ggatgtccc cgatgcggc 
      ggttacgtg tacgctgcc gaacagatg ttagcaaga 
      ggaagagcg ttccacaaa acctgtttc aaatgtgcc 
      gtgtgtaac aaaggattg gattctgtt aatgtctct 
      gaaggtccc gacaaggat atttattgc aaagtttgt 
      tacggaaag aaatttgga ccgaaagga tatggttac 
      ggccaaggt ggtggcacc cttcaaagc gattgttac 
      gccgttagt gatgccgcc cccaaaacc acggtgatt 
      gacaccgct tgcatcaag gctcctcca ggccaaggt 
      tgtccccgt tgtggtggt gtagtgttt gccgccgaa 
      gaggtcctc gcgaaagga agaccttgg catcgtaaa 
      tgtttcaaa tgcaaggat tgtactaag accctcgac 
      tcaattatc gcttgcgat ggacctgat agcgatgtc 
      tattgtaag acctgttac ggcaaaaag tggggccct 
      cacggatac ggcttcgct tgtggctca ggttttctc 
      cagactgat gggctaacc gaagatgaa atttcgcaa 
      acacgacct ttctataat cccgatacg acctctatc 
      aaggcccct ccaggacaa ggatgcccc cgatgtgga 
      ggaatggtc ttcgctgcc gagcaacaa ctggccaaa 
      ggaacaatg tggcacaag aagtgcttc aactgcgcc 
      gagtgtcat cgtcctctc gattcagtc ctggcttgt 
      gacggtcct gaccgtgaa atccactgc agggcctgc 
      tacagcaag ctgtttggt cccaaaggg ttcggtttt 
      ggccacact cccactctg gtgtgtgcc gacggcgcc 
      cctgccgcc atccacaac gacatcaga cccaacagc 
      gggccaaaa gccgcccct gggcaaggc tgccgtcgt 
      tgtggttat gccgtctat gctgctgag cagatgatc 
      agcaaaaac ggcatctgg cacaagagg tgcttctgc 
      tgtgttgaa tgcaagcgc tcgttggat tccaccaac 
      caaaacgat gcccccgat ggcgaaatt tactgccga 
      ggttgctac ggaaggaat ttcgggccc aagggagtt 
      ggttttgga atgggcgct ggcactctg actatggct 
      tag
    • >TC007136 RC
      atggctaaa ggcaatctg ctcttctcg ctattaaca 
      tacgacgat attgttgtt tctcgagcc ccccttttg 
      ccgaaagcc atcgcgaag gcccccgaa ggcgaagga 
      tgtccccga tgcggcggt tacgtgtac gctgccgaa 
      cagatgtta gcaagagga agagcgttc cacaaaacc 
      tgtttcaaa tgtgccgtg tgtaacaaa ggattggat 
      tctgttaat gtctctgaa ggtcccgac aaggatatt 
      tattgcaaa gtttgttac ggaaagaaa tttggaccg 
      aaaggatat ggttacggc caaggtggt ggcaccctt 
      caaagcgat tgttacgcc gttagtgat gccgccccc 
      aaaaccacg gtgattgac accgcttgc atcaaggct 
      cctccaggc caaggttgt ccccgttgt ggtggtgta 
      gtgtttgcc gccgaagag gtcctcgcg aaaggaaga 
      ccttggcat cgtaaatgt ttcaaatgc aaggattgt 
      actaagacc ctcgactca attatcgct tgcgatgga 
      cctgatagc gatgtctat tgtaagacc tgttacggc 
      aaaaagtgg ggccctcac ggatacggc ttcgcttgt 
      ggctcaggt tttctccag actgatggg ctaaccgaa 
      gatgaaatt tcgcaaaca cgacctttc tataatccc 
      gatacgacc tctatcaag gcccctcca ggacaagga 
      tgcccccga tgtggagga atggtcttc gctgccgag 
      caacaactg gccaaagga acaatgtgg cacaagaag 
      tgcttcaac tgcgccgag tgtcatcgt cctctcgat 
      tcagtcctg gcttgtgac ggtcctgac cgtgaaatc 
      cactgcagg gcctgctac agcaagctg tttggtccc 
      aaagggttc ggttttggc cacactccc actctggtg 
      tgtgccgac ggcgcccct gccgccatc cacaacgac 
      atcagaccc aacagcggg ccaaaagcc gcccctggg 
      caaggctgc cgtcgttgt ggttatgcc gtctatgct 
      gctgagcag atgatcagc aaaaacggc atctggcac 
      aagaggtgc ttctgctgt gttgaatgc aagcgctcg 
      ttggattcc accaaccaa aacgatgcc cccgatggc 
      gaaatttac tgccgaggt tgctacgga aggaatttc 
      gggcccaag ggagttggt tttggaatg ggcgctggc 
      actctgact atggcttag
    mRNA sequence
    • >TC007136 RA
      ttcgaattt cagtggttc tagtgtaag gacaccatt 
      gaggacctc tccaccgac tactgtacg attccgacc 
      aggtcgact agatcaaga tgcctttca agcctgccg 
      aaaacccca aatgcccca agtgccaga aatccgtct 
      acgccgctg aagagcgtg tagctggcg gttacaaat 
      ggcacaagt cctgcttca aatgcggcc tttgcggca 
      aaatgttgg actccacca actgcacag aacacgaag 
      ccgaattgt attgcaaga actgccacg cccgcaaat 
      acggaccta aaggctacg gctttggcg gtggtgccg 
      gttgcctta gcatggaca ctggctccc atctccaac 
      aaggctcct aaggaatca gccggggac agaagagcg 
      gccttgtca ccaacaaca ccatcgccg cctcacaaa 
      acctactcc ttccatccc acgtataat taattatat 
      gttgtgtta tatttatag attttacaa taacaaata 
      ggttactgt ttataaaat ttgttttac atggtacaa 
      acaattcga ttattattt tgtaataat tgaatcgta 
      ataaatcac gtcaaaaat t
    • >TC007136 RB
      ttcgaattt cagtggttc tagtgtaag gacaccatt 
      gaggacctc tccaccgac tactgtacg attccgacc 
      aggtcgact agatcaaga tgcctttca agcctgccg 
      aaaacccca aatgcccca agtgccaga aatccgtct 
      acgccgctg aagagcgtg tagctggcg gttacaaat 
      ggcacaagt cctgcttca aatgcggcc tttgcggca 
      aaatgttgg actccacca actgcacag aacacgaag 
      ccgaattgt attgcaaga actgccacg cccgcaaat 
      acggaccta aaggctacg gctttggcg gtggtgccg 
      gttgcctta gcatggaca ctggctccc atctccaac 
      aaggcgacg atattgttg tttctcgag ccccccttt 
      tgccgaaag ccatcgcga aggcccccg aaggcgaag 
      gatgtcccc gatgcggcg gttacgtgt acgctgccg 
      aacagatgt tagcaagag gaagagcgt tccacaaaa 
      cctgtttca aatgtgccg tgtgtaaca aaggattgg 
      attctgtta atgtctctg aaggtcccg acaaggata 
      tttattgca aagtttgtt acggaaaga aatttggac 
      cgaaaggat atggttacg gccaaggtg gtggcaccc 
      ttcaaagcg attgttacg ccgttagtg atgccgccc 
      ccaaaacca cggtgattg acaccgctt gcatcaagg 
      ctcctccag gccaaggtt gtccccgtt gtggtggtg 
      tagtgtttg ccgccgaag aggtcctcg cgaaaggaa 
      gaccttggc atcgtaaat gtttcaaat gcaaggatt 
      gtactaaga ccctcgact caattatcg cttgcgatg 
      gacctgata gcgatgtct attgtaaga cctgttacg 
      gcaaaaagt ggggccctc acggatacg gcttcgctt 
      gtggctcag gttttctcc agactgatg ggctaaccg 
      aagatgaaa tttcgcaaa cacgacctt tctataatc 
      ccgatacga cctctatca aggcccctc caggacaag 
      gatgccccc gatgtggag gaatggtct tcgctgccg 
      agcaacaac tggccaaag gaacaatgt ggcacaaga 
      agtgcttca actgcgccg agtgtcatc gtcctctcg 
      attcagtcc tggcttgtg acggtcctg accgtgaaa 
      tccactgca gggcctgct acagcaagc tgtttggtc 
      ccaaagggt tcggttttg gccacactc ccactctgg 
      tgtgtgccg acggcgccc ctgccgcca tccacaacg 
      acatcagac ccaacagcg ggccaaaag ccgcccctg 
      ggcaaggct gccgtcgtt gtggttatg ccgtctatg 
      ctgctgagc agatgatca gcaaaaacg gcatctggc 
      acaagaggt gcttctgct gtgttgaat gcaagcgct 
      cgttggatt ccaccaacc aaaacgatg cccccgatg 
      gcgaaattt actgccgag gttgctacg gaaggaatt 
      tcgggccca agggagttg gttttggaa tgggcgctg 
      gcactctga ctatggctt agattcacc gacccgacc 
      ctttgaact ctcttaacc gatgcggat tggttagga 
      ctgttccac cttttagct tgtggtttt agcaggaat 
      aatattatt actaaaaat gatgcttga ggacgcata 
      ttttttttg gagattttt tggaattcc atttttgat 
      agaggacac gacagaaag ggaacaaga ctcgaacgg 
      cttattccg ttaccagtt ggtataaga ttatgaaag 
      acttcctcg aacgaccag ggttttcct gaggttttt 
      tgtacatat ctacactgg ccacaatgt tggtctaat 
      atagcttcg cgctttgct actgtactc gatttgttt 
      ttatgctat ttacaacgt aagtctttt tgtctagat 
      gtactttca ttgtatatt atgtttaat aaaaagcta 
      ataaagtta cctaaaaaa c
    • >TC007136 RC
      agaacggtg agacaatag atgatttaa gcatggcta 
      aaggcaatc tgctcttct cgctattaa catacgacg 
      atattgttg tttctcgag ccccccttt tgccgaaag 
      ccatcgcga aggcccccg aaggcgaag gatgtcccc 
      gatgcggcg gttacgtgt acgctgccg aacagatgt 
      tagcaagag gaagagcgt tccacaaaa cctgtttca 
      aatgtgccg tgtgtaaca aaggattgg attctgtta 
      atgtctctg aaggtcccg acaaggata tttattgca 
      aagtttgtt acggaaaga aatttggac cgaaaggat 
      atggttacg gccaaggtg gtggcaccc ttcaaagcg 
      attgttacg ccgttagtg atgccgccc ccaaaacca 
      cggtgattg acaccgctt gcatcaagg ctcctccag 
      gccaaggtt gtccccgtt gtggtggtg tagtgtttg 
      ccgccgaag aggtcctcg cgaaaggaa gaccttggc 
      atcgtaaat gtttcaaat gcaaggatt gtactaaga 
      ccctcgact caattatcg cttgcgatg gacctgata 
      gcgatgtct attgtaaga cctgttacg gcaaaaagt 
      ggggccctc acggatacg gcttcgctt gtggctcag 
      gttttctcc agactgatg ggctaaccg aagatgaaa 
      tttcgcaaa cacgacctt tctataatc ccgatacga 
      cctctatca aggcccctc caggacaag gatgccccc 
      gatgtggag gaatggtct tcgctgccg agcaacaac 
      tggccaaag gaacaatgt ggcacaaga agtgcttca 
      actgcgccg agtgtcatc gtcctctcg attcagtcc 
      tggcttgtg acggtcctg accgtgaaa tccactgca 
      gggcctgct acagcaagc tgtttggtc ccaaagggt 
      tcggttttg gccacactc ccactctgg tgtgtgccg 
      acggcgccc ctgccgcca tccacaacg acatcagac 
      ccaacagcg ggccaaaag ccgcccctg ggcaaggct 
      gccgtcgtt gtggttatg ccgtctatg ctgctgagc 
      agatgatca gcaaaaacg gcatctggc acaagaggt 
      gcttctgct gtgttgaat gcaagcgct cgttggatt 
      ccaccaacc aaaacgatg cccccgatg gcgaaattt 
      actgccgag gttgctacg gaaggaatt tcgggccca 
      agggagttg gttttggaa tgggcgctg gcactctga 
      ctatggctt agattcacc gacccgacc ctttgaact 
      ctcttaacc gatgcggat tggttagga ctgttccac 
      cttttagct tgtggtttt agcaggaat aatattatt 
      actaaaaat gatgcttga ggacgcata ttttttttg 
      gagattttt tggaattcc atttttgat agaggacac 
      gacagaaag ggaacaaga ctcgaacgg cttattccg 
      ttaccagtt ggtataaga ttatgaaag acttcctcg 
      aacgaccag ggttttcct gaggttttt tgtacatat 
      ctacactgg ccacaatgt tggtctaat atagcttcg 
      cgctttgct actgtactc gatttgttt ttatgctat 
      ttacaacgt aagtctttt tgtctagat gtactttca 
      ttgtatatt atgtttaat aaaaagcta ataaagtta 
      cctaaaaaa c