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Gene information
General information
TC007106 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 9425182..9430738
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007106
    iBeetle screen iB_01125
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 96
    Pupal morphology
    remarks: later than schedule, hours 96
    Adult morphology
    remarks: later than schedule, hours 96
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007106 RA
      MSSFEAVQE FYVSNFVNI RSKKRNVFT CKFPRHWLI 
      VLVLSTIIF LIIVNFVIP YNSVYKDLS SNTKLAYVS 
      KEDEGQWNG QKKTHPLKE TDKNPLHDK CETIHIGFV 
      CSGYRSNLY LHTLLKSLF FYNINPIHF HIMVNKVSE 
      KVLKTLFDT WNVPQSNTT FYDINDYIQ DVRWVPNSH 
      YSGIFGLLK LVFPKIIPL NVTKKIIVL DTDLTFVGD 
      IIELWRLFE KFNNKQAVG IVENQSDYY LGKNTKIKP 
      WPASGRGFN SGVLLYNLE RLRRLDWPS LWPSVAKKV 
      AIIYGSTRL GDQDIINAV LLQHTDLLY QVPCFWNTQ 
      LSDHALSYN CYNKFRVKV VHWNSPKKT HVLNKDGDY 
      FRTLYQTFV EYNGNLLRR QLYYCDATQ DAVNEPQTD 
      LCAEFHRAK TSQWRTLLF FREYQHSAE ENAKNDVTF 
      VAQLSYDRL QMVEELVKY WQGPISLTF YVTDPEFQQ 
      CYNFIENSE LLQDRTNIA YHAVFKDGE YHPINILRN 
      VGLKNVATP FVFLADIDF LPMKGLYSV LKSHLNSIH 
      DMKAKALIV PAFETQRYR SRVPKNKAQ LLGMWEKKS 
      IFPFRSDVW VAGHAPTNY TKWKSAIAP YKVKWEPDF 
      EPYIVVSSD VTEYDNRFM GFGWNKVSH IMELEAQGY 
      QWIVLPNAF IIHKPHAPS YDIAKFRTS PVYRMCLQN 
      LKEEFIKDL DKKYGRLFD NKNTSFLLD IK
    CDS sequence
    • >TC007106 RA
      atgtcaagt tttgaggcg gttcaggaa ttttatgtc 
      tcgaatttt gtaaatata cgttcaaaa aaacgcaat 
      gtattcact tgtaaattt cctcgacac tggctaatt 
      gttttagta ttgtctact ataattttt cttataatt 
      gtgaacttt gttattcct tacaattca gtttacaaa 
      gacctcagc agtaatacc aagctcgca tatgtctca 
      aaggaggat gaaggccaa tggaatggg caaaaaaag 
      acccatcct ttgaaagaa acggacaaa aacccactt 
      catgataaa tgtgaaaca attcacatt gggtttgtt 
      tgttctgga tacaggtct aatttatac ttgcacact 
      ttactaaaa tcattgttt ttttacaac atcaatccc 
      atccatttt catattatg gtcaacaag gtctctgaa 
      aaagtcctg aaaacttta ttcgacaca tggaacgtg 
      ccacaaagc aatacaaca ttttacgac attaatgat 
      tacattcag gacgttaga tgggtcccc aacagccat 
      tattccggc atttttggc ttgctaaag cttgtgttt 
      ccaaaaatt attccttta aacgttacc aagaaaatc 
      atagtcctt gacactgat ttaactttc gttggcgac 
      attatcgag ctgtggaga ctgtttgaa aaattcaat 
      aataaacaa gcagttgga attgttgaa aatcagagc 
      gattactat ttggggaaa aacaccaaa attaagcct 
      tggcccgcc agcggcaga gggtttaat tctggcgta 
      ttgctttac aatttagaa agacttagg agactggat 
      tggcctagc ttgtggccc tctgttgcc aaaaaagta 
      gcgataatt tacggttca acccgtttg ggagatcaa 
      gacataata aacgcggtg ttattgcaa cacactgat 
      ctcctgtac caagtgcct tgcttctgg aatactcaa 
      ctaagtgat cacgcttta agttacaac tgttacaat 
      aagtttcga gttaaagta gtccattgg aattcgcca 
      aaaaaaact cacgttctt aataaagac ggtgattat 
      tttcgcact ttgtaccag acttttgtt gaatataat 
      ggaaatttg ttacgacgc cagctgtat tattgcgat 
      gcgactcaa gacgctgtt aatgaacca caaaccgat 
      ctttgtgca gagttccat cgcgccaaa actagtcag 
      tggagaact ttactattt tttagggag tatcaacat 
      tcagccgag gaaaatgcc aaaaatgat gttactttt 
      gttgcacaa ttgtcttat gataggtta caaatggta 
      gaagagttg gttaagtac tggcaaggc cctattagt 
      ctcacgttt tacgttacc gatcctgag tttcaacaa 
      tgttacaat tttatagaa aattcggaa ttattgcag 
      gaccggact aacatagcg taccatgct gttttcaaa 
      gatggggaa tatcaccca atcaacatt ttacgcaac 
      gttggatta aaaaatgta gccacccct tttgttttc 
      ttggccgac attgacttt ctaccaatg aaaggttta 
      tattcagta ttgaagagt cacctgaat tccattcac 
      gacatgaaa gccaaagca ttaatagtt cctgcgttt 
      gaaacccaa agatatcgt agtcgtgtt ccgaaaaac 
      aaagcccaa ttgttggga atgtgggag aaaaaatct 
      attttcccg ttcaggtcc gatgtgtgg gttgccgga 
      catgcacct actaattac acaaaatgg aaatcggca 
      attgcaccc tacaaagtc aagtgggag cccgatttt 
      gagccgtat atcgtggtc agtagtgac gtcactgaa 
      tatgataat agattcatg ggttttggg tggaataag 
      gtgtctcat attatggag ttggaagcg cagggctac 
      caatggatt gttttaccg aatgcattt attattcac 
      aaaccacat gcgcctagc tatgatata gccaagttt 
      cgaacttct ccggtttac agaatgtgt ttgcaaaac 
      ttgaaggaa gagtttatc aaagatttg gacaaaaaa 
      tacggtaga ttgttcgat aataaaaac acatcgttt 
      cttttagat attaagtag
    mRNA sequence
    • >TC007106 RA
      agaaattga agtttgtgc tttcacgtc aaatgtcaa 
      gttttgagg cggttcagg aattttatg tctcgaatt 
      ttgtaaata tacgttcaa aaaaacgca atgtattca 
      cttgtaaat ttcctcgac actggctaa ttgttttag 
      tattgtcta ctataattt ttcttataa ttgtgaact 
      ttgttattc cttacaatt cagtttaca aagacctca 
      gcagtaata ccaagctcg catatgtct caaaggagg 
      atgaaggcc aatggaatg ggcaaaaaa agacccatc 
      ctttgaaag aaacggaca aaaacccac ttcatgata 
      aatgtgaaa caattcaca ttgggtttg tttgttctg 
      gatacaggt ctaatttat acttgcaca ctttactaa 
      aatcattgt ttttttaca acatcaatc ccatccatt 
      ttcatatta tggtcaaca aggtctctg aaaaagtcc 
      tgaaaactt tattcgaca catggaacg tgccacaaa 
      gcaatacaa cattttacg acattaatg attacattc 
      aggacgtta gatgggtcc ccaacagcc attattccg 
      gcatttttg gcttgctaa agcttgtgt ttccaaaaa 
      ttattcctt taaacgtta ccaagaaaa tcatagtcc 
      ttgacactg atttaactt tcgttggcg acattatcg 
      agctgtgga gactgtttg aaaaattca ataataaac 
      aagcagttg gaattgttg aaaatcaga gcgattact 
      atttgggga aaaacacca aaattaagc cttggcccg 
      ccagcggca gagggttta attctggcg tattgcttt 
      acaatttag aaagactta ggagactgg attggccta 
      gcttgtggc cctctgttg ccaaaaaag tagcgataa 
      tttacggtt caacccgtt tgggagatc aagacataa 
      taaacgcgg tgttattgc aacacactg atctcctgt 
      accaagtgc cttgcttct ggaatactc aactaagtg 
      atcacgctt taagttaca actgttaca ataagtttc 
      gagttaaag tagtccatt ggaattcgc caaaaaaaa 
      ctcacgttc ttaataaag acggtgatt attttcgca 
      ctttgtacc agacttttg ttgaatata atggaaatt 
      tgttacgac gccagctgt attattgcg atgcgactc 
      aagacgctg ttaatgaac cacaaaccg atctttgtg 
      cagagttcc atcgcgcca aaactagtc agtggagaa 
      ctttactat tttttaggg agtatcaac attcagccg 
      aggaaaatg ccaaaaatg atgttactt ttgttgcac 
      aattgtctt atgataggt tacaaatgg tagaagagt 
      tggttaagt actggcaag gccctatta gtctcacgt 
      tttacgtta ccgatcctg agtttcaac aatgttaca 
      attttatag aaaattcgg aattattgc aggaccgga 
      ctaacatag cgtaccatg ctgttttca aagatgggg 
      aatatcacc caatcaaca ttttacgca acgttggat 
      taaaaaatg tagccaccc cttttgttt tcttggccg 
      acattgact ttctaccaa tgaaaggtt tatattcag 
      tattgaaga gtcacctga attccattc acgacatga 
      aagccaaag cattaatag ttcctgcgt ttgaaaccc 
      aaagatatc gtagtcgtg ttccgaaaa acaaagccc 
      aattgttgg gaatgtggg agaaaaaat ctattttcc 
      cgttcaggt ccgatgtgt gggttgccg gacatgcac 
      ctactaatt acacaaaat ggaaatcgg caattgcac 
      cctacaaag tcaagtggg agcccgatt ttgagccgt 
      atatcgtgg tcagtagtg acgtcactg aatatgata 
      atagattca tgggttttg ggtggaata aggtgtctc 
      atattatgg agttggaag cgcagggct accaatgga 
      ttgttttac cgaatgcat ttattattc acaaaccac 
      atgcgccta gctatgata tagccaagt ttcgaactt 
      ctccggttt acagaatgt gtttgcaaa acttgaagg 
      aagagttta tcaaagatt tggacaaaa aatacggta 
      gattgttcg ataataaaa acacatcgt ttcttttag 
      atattaagt agaacagtt ttaaacgca ccatcgggg 
      aaggggtca taaagctac caagtcatt gctagtgtg 
      atgtttatt cttttaatt aggcaacta gtaggtgta 
      taagtctca cgttggcaa tcaaatcta attttacta 
      aacttatct ctgtgaaat atttattct tccatgtaa 
      ggtgtagat attttaaat aaatttatc gatcagaa