| General information | |||
| TC007101 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9551712..9570201 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007101 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01124 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC007101 RA
MFPKCVSGA STVSQLPKL FFLFSVSQL VSSSAVPED WVSGSPFSH FAPDDSRAD TFTSAYLNV SNDGVRWDK TEVGRYGGG YVGPAFGVL VHVAAENNP DDHTGCKLP LHSTRSNRK LPPPGEPWI ALIKRGRCN FEVKVENAF RSNAAGVLV YNDRDSATL DKMKLSSDS GRNISAVFT YKWKGEDLA RLAENDSKV YIHITIASH TSSRTANIN RTSVLFVSI TFIVLMIIS LAWLVFYYV QRFRYIHAK DRLSRRLGN AAKKALSKI PTKNIKSED KEVQGDGEC CAICIEPYK ICDILRILP CGHEFHKSC IDPWLLEHR TCPMCKMDI LKHYGFVFS GSQESILQM DVEEIVVLD STESRSTSP RRGGGISPL PEIRAVVIA DRQRLFESS ADDESSRSS TPDEMTPSL SQARQYPVR QDLCVSCIA AKAAAALSS SQSKEMSTS DEEQDSNRP VLSVNNQSD DK
- >TC007101 RA
atgttcccc aagtgtgtt agtggtgca agcaccgtg tcccagctc cccaaactg ttctttttg ttctccgtt agccagttg gtgtcttct tcagcagtg cccgaggac tgggtgtcg ggatccccc ttctcgcac ttcgcgccg gacgacagc agggccgac actttcacg tcggcttac ctgaacgtg tccaacgat ggagtgcgt tgggacaag acggaggtg ggccgctat gggggaggc tacgtgggg cccgccttc ggggtgctg gtgcacgtg gcggccgag aacaacccc gacgaccac accgggtgc aagctcccg ctgcacagc accaggtcc aatcggaag ttgccgccg ccaggggag ccctggatc gccttgatc aagagaggc cgctgcaac tttgaagtg aaggtcgag aacgccttc aggagcaac gctgcgggg gttctggtc tataatgat agggactcc gcgactttg gacaagatg aagttgtcc agtgactcc ggacgtaat ataagcgcc gtgtttacc tacaaatgg aagggagaa gacttggcc aggttggct gaaaatgat tccaaagtt tacattcac atcactatt gcgagccac acgtcctca cggactgcc aacatcaat agaacatct gtgttattt gtctccatc acgtttatt gttttgatg atcatatcc ttggcgtgg ttggtgttc tactacgtg cagaggttt agatatatc cacgcaaaa gacagactg tcgcgaaga ctgggaaat gcagcaaag aaagcgtta tctaaaatc ccaacgaaa aacatcaaa tccgaggac aaagaggtt caaggcgac ggggaatgt tgcgctatt tgcattgaa ccttacaaa atatgtgat attttacgc atattacct tgcggtcat gagttccat aagagctgc attgatcct tggttgctg gaacatcga acatgtcca atgtgtaaa atggacatc ctcaagcat tatggattt gtgttttcc ggtagtcaa gaaagcatc ctacagatg gacgtggag gaaatcgta gtgttagac tcgacagag agtcgttca actagtcca agaaggggc ggcggtatc agtcctttg ccggagata cgggccgtt gtcatcgct gaccgtcag cgcctgttc gagagttcg gcggacgac gaaagcagc agatcttcc acccctgac gaaatgact ccgagttta tcgcaggca agacagtac cccgtcaga caggacctt tgcgtgagc tgtatagcg gcaaaggcg gccgcggcc ctgtcgagc agccaaagc aaggaaatg tcaacttct gacgaggaa caagactcc aaccggccg gtgctttca gtgaacaat caaagtgat gataaataa
- >TC007101 RA
attcagttg tgtggaaca cttgagcga gacggttgt ggagcccca gccgtgcgc gagttcgtc gacttcaca ggaaaagtg ctgtttgaa caaagaacg tcacttttc agtgtggaa agtgcccct ccagcatga atgttccca tctaacccg cgaagatgt tccccaagt gtgttagtg gtgcaagca ccgtgtccc agctcccca aactgttct ttttgttct ccgttagcc agttggtgt cttcttcag cagtgcccg aggactggg tgtcgggat cccccttct cgcacttcg cgccggacg acagcaggg ccgacactt tcacgtcgg cttacctga acgtgtcca acgatggag tgcgttggg acaagacgg aggtgggcc gctatgggg gaggctacg tggggcccg ccttcgggg tgctggtgc acgtggcgg ccgagaaca accccgacg accacaccg ggtgcaagc tcccgctgc acagcacca ggtccaatc ggaagttgc cgccgccag gggagccct ggatcgcct tgatcaaga gaggccgct gcaactttg aagtgaagg tcgagaacg ccttcagga gcaacgctg cgggggttc tggtctata atgataggg actccgcga ctttggaca agatgaagt tgtccagtg actccggac gtaatataa gcgccgtgt ttacctaca aatggaagg gagaagact tggccaggt tggctgaaa atgattcca aagtttaca ttcacatca ctattgcga gccacacgt cctcacgga ctgccaaca tcaatagaa catctgtgt tatttgtct ccatcacgt ttattgttt tgatgatca tatccttgg cgtggttgg tgttctact acgtgcaga ggtttagat atatccacg caaaagaca gactgtcgc gaagactgg gaaatgcag caaagaaag cgttatcta aaatcccaa cgaaaaaca tcaaatccg aggacaaag aggttcaag gcgacgggg aatgttgcg ctatttgca ttgaacctt acaaaatat gtgatattt tacgcatat taccttgcg gtcatgagt tccataaga gctgcattg atccttggt tgctggaac atcgaacat gtccaatgt gtaaaatgg acatcctca agcattatg gatttgtgt tttccggta gtcaagaaa gcatcctac agatggacg tggaggaaa tcgtagtgt tagactcga cagagagtc gttcaacta gtccaagaa ggggcggcg gtatcagtc ctttgccgg agatacggg ccgttgtca tcgctgacc gtcagcgcc tgttcgaga gttcggcgg acgacgaaa gcagcagat cttccaccc ctgacgaaa tgactccga gtttatcgc aggcaagac agtaccccg tcagacagg acctttgcg tgagctgta tagcggcaa aggcggccg cggccctgt cgagcagcc aaagcaagg aaatgtcaa cttctgacg aggaacaag actccaacc ggccggtgc tttcagtga acaatcaaa gtgatgata aataagaca gttttctat tacggtggt gcagcagta gtcgttagg actgccgaa actttgtga tatatttct agtggttaa gtttttatt ttgtccaat acgaataaa gggtgcttt cgtcccaaa tactaacaa taatttatc gttcgctag aattcatat tctagtgta agtaaatag atctctcgc gacgagttg attccaatt ttccaagag attacatcc aacttattg ggcttggga actcaatca agactcctg ttttgtttg actatttgt tatcaagcg aacgaatga cgcgtttct gaaggataa caagctgtt actttaatc gaaaataaa ttaggagtt ttatgattt tgcttcgcc caagcgtga ctttagtat gaagcgacg tgcttaata tttaaacta ctgttcgca gtagtgctt ccaattcac attgttatt gtctgctgg acaatgttt gtttgtgga aaacgaatt ttcttctat aaacgttat aattacaat acaaatctt aatactgtg ccttgtaaa tatctacat ttgtgatgt acgtgatgc cccgcacga tgtaggtat gtgagcagt tttgttatt tatctgtga ttgttaacg tttgttagg ggcgtcgtg tatactttt tctgagact tacaaccga gcatgtact tcatcttat tttgcttgt aaatattat gctaaaatt gaaattgac tgaattaaa actctagat atgaatcaa tgtgtttta ttgttttga ttacaatgt tatgtcacg cgttaagat tgttaaata taaaaaata tacatcaag aaacgacta gaaggagac tagacctct taattaaga taatataaa aatatcaat tgcatcaca ttgaaaata gttaggcaa acacaaacc aaattccac tggcagtaa taaaataag tcagacttg ttttaaaaa taagttgaa aaaccgatc acttcgtga atattgaaa ctagaagtt atgtaaaat tttcgatgg ttttttgtc tctcggacc acgatgttc gtaattgga tgtttccca attatgact ctttttgca tattccttg attgtgaag tgtttccac gaggggacg tggtaaaaa tacacctaa aaaacaatg aaaatcgga actacggag gattaaatg ttaggagat acgaccccg cgtacaaat caatcaatt tttttttaa ttatgaaga atatttcaa aaatgtgac gcatggtaa taaacagca aacagaacc
