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Gene information
General information
TC007101 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9551712..9570201
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007101
    iBeetle screen iB_01124

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007101 RA
      MFPKCVSGA STVSQLPKL FFLFSVSQL VSSSAVPED 
WVSGSPFSH FAPDDSRAD TFTSAYLNV SNDGVRWDK 
TEVGRYGGG YVGPAFGVL VHVAAENNP DDHTGCKLP 
LHSTRSNRK LPPPGEPWI ALIKRGRCN FEVKVENAF 
RSNAAGVLV YNDRDSATL DKMKLSSDS GRNISAVFT 
YKWKGEDLA RLAENDSKV YIHITIASH TSSRTANIN 
RTSVLFVSI TFIVLMIIS LAWLVFYYV QRFRYIHAK 
DRLSRRLGN AAKKALSKI PTKNIKSED KEVQGDGEC 
CAICIEPYK ICDILRILP CGHEFHKSC IDPWLLEHR 
TCPMCKMDI LKHYGFVFS GSQESILQM DVEEIVVLD 
STESRSTSP RRGGGISPL PEIRAVVIA DRQRLFESS 
ADDESSRSS TPDEMTPSL SQARQYPVR QDLCVSCIA 
AKAAAALSS SQSKEMSTS DEEQDSNRP VLSVNNQSD 
DK
    CDS sequence
    • >TC007101 RA
      atgttcccc aagtgtgtt agtggtgca agcaccgtg 
tcccagctc cccaaactg ttctttttg ttctccgtt 
agccagttg gtgtcttct tcagcagtg cccgaggac 
tgggtgtcg ggatccccc ttctcgcac ttcgcgccg 
gacgacagc agggccgac actttcacg tcggcttac 
ctgaacgtg tccaacgat ggagtgcgt tgggacaag 
acggaggtg ggccgctat gggggaggc tacgtgggg 
cccgccttc ggggtgctg gtgcacgtg gcggccgag 
aacaacccc gacgaccac accgggtgc aagctcccg 
ctgcacagc accaggtcc aatcggaag ttgccgccg 
ccaggggag ccctggatc gccttgatc aagagaggc 
cgctgcaac tttgaagtg aaggtcgag aacgccttc 
aggagcaac gctgcgggg gttctggtc tataatgat 
agggactcc gcgactttg gacaagatg aagttgtcc 
agtgactcc ggacgtaat ataagcgcc gtgtttacc 
tacaaatgg aagggagaa gacttggcc aggttggct 
gaaaatgat tccaaagtt tacattcac atcactatt 
gcgagccac acgtcctca cggactgcc aacatcaat 
agaacatct gtgttattt gtctccatc acgtttatt 
gttttgatg atcatatcc ttggcgtgg ttggtgttc 
tactacgtg cagaggttt agatatatc cacgcaaaa 
gacagactg tcgcgaaga ctgggaaat gcagcaaag 
aaagcgtta tctaaaatc ccaacgaaa aacatcaaa 
tccgaggac aaagaggtt caaggcgac ggggaatgt 
tgcgctatt tgcattgaa ccttacaaa atatgtgat 
attttacgc atattacct tgcggtcat gagttccat 
aagagctgc attgatcct tggttgctg gaacatcga 
acatgtcca atgtgtaaa atggacatc ctcaagcat 
tatggattt gtgttttcc ggtagtcaa gaaagcatc 
ctacagatg gacgtggag gaaatcgta gtgttagac 
tcgacagag agtcgttca actagtcca agaaggggc 
ggcggtatc agtcctttg ccggagata cgggccgtt 
gtcatcgct gaccgtcag cgcctgttc gagagttcg 
gcggacgac gaaagcagc agatcttcc acccctgac 
gaaatgact ccgagttta tcgcaggca agacagtac 
cccgtcaga caggacctt tgcgtgagc tgtatagcg 
gcaaaggcg gccgcggcc ctgtcgagc agccaaagc 
aaggaaatg tcaacttct gacgaggaa caagactcc 
aaccggccg gtgctttca gtgaacaat caaagtgat 
gataaataa
    mRNA sequence
    • >TC007101 RA
      attcagttg tgtggaaca cttgagcga gacggttgt 
ggagcccca gccgtgcgc gagttcgtc gacttcaca 
ggaaaagtg ctgtttgaa caaagaacg tcacttttc 
agtgtggaa agtgcccct ccagcatga atgttccca 
tctaacccg cgaagatgt tccccaagt gtgttagtg 
gtgcaagca ccgtgtccc agctcccca aactgttct 
ttttgttct ccgttagcc agttggtgt cttcttcag 
cagtgcccg aggactggg tgtcgggat cccccttct 
cgcacttcg cgccggacg acagcaggg ccgacactt 
tcacgtcgg cttacctga acgtgtcca acgatggag 
tgcgttggg acaagacgg aggtgggcc gctatgggg 
gaggctacg tggggcccg ccttcgggg tgctggtgc 
acgtggcgg ccgagaaca accccgacg accacaccg 
ggtgcaagc tcccgctgc acagcacca ggtccaatc 
ggaagttgc cgccgccag gggagccct ggatcgcct 
tgatcaaga gaggccgct gcaactttg aagtgaagg 
tcgagaacg ccttcagga gcaacgctg cgggggttc 
tggtctata atgataggg actccgcga ctttggaca 
agatgaagt tgtccagtg actccggac gtaatataa 
gcgccgtgt ttacctaca aatggaagg gagaagact 
tggccaggt tggctgaaa atgattcca aagtttaca 
ttcacatca ctattgcga gccacacgt cctcacgga 
ctgccaaca tcaatagaa catctgtgt tatttgtct 
ccatcacgt ttattgttt tgatgatca tatccttgg 
cgtggttgg tgttctact acgtgcaga ggtttagat 
atatccacg caaaagaca gactgtcgc gaagactgg 
gaaatgcag caaagaaag cgttatcta aaatcccaa 
cgaaaaaca tcaaatccg aggacaaag aggttcaag 
gcgacgggg aatgttgcg ctatttgca ttgaacctt 
acaaaatat gtgatattt tacgcatat taccttgcg 
gtcatgagt tccataaga gctgcattg atccttggt 
tgctggaac atcgaacat gtccaatgt gtaaaatgg 
acatcctca agcattatg gatttgtgt tttccggta 
gtcaagaaa gcatcctac agatggacg tggaggaaa 
tcgtagtgt tagactcga cagagagtc gttcaacta 
gtccaagaa ggggcggcg gtatcagtc ctttgccgg 
agatacggg ccgttgtca tcgctgacc gtcagcgcc 
tgttcgaga gttcggcgg acgacgaaa gcagcagat 
cttccaccc ctgacgaaa tgactccga gtttatcgc 
aggcaagac agtaccccg tcagacagg acctttgcg 
tgagctgta tagcggcaa aggcggccg cggccctgt 
cgagcagcc aaagcaagg aaatgtcaa cttctgacg 
aggaacaag actccaacc ggccggtgc tttcagtga 
acaatcaaa gtgatgata aataagaca gttttctat 
tacggtggt gcagcagta gtcgttagg actgccgaa 
actttgtga tatatttct agtggttaa gtttttatt 
ttgtccaat acgaataaa gggtgcttt cgtcccaaa 
tactaacaa taatttatc gttcgctag aattcatat 
tctagtgta agtaaatag atctctcgc gacgagttg 
attccaatt ttccaagag attacatcc aacttattg 
ggcttggga actcaatca agactcctg ttttgtttg 
actatttgt tatcaagcg aacgaatga cgcgtttct 
gaaggataa caagctgtt actttaatc gaaaataaa 
ttaggagtt ttatgattt tgcttcgcc caagcgtga 
ctttagtat gaagcgacg tgcttaata tttaaacta 
ctgttcgca gtagtgctt ccaattcac attgttatt 
gtctgctgg acaatgttt gtttgtgga aaacgaatt 
ttcttctat aaacgttat aattacaat acaaatctt 
aatactgtg ccttgtaaa tatctacat ttgtgatgt 
acgtgatgc cccgcacga tgtaggtat gtgagcagt 
tttgttatt tatctgtga ttgttaacg tttgttagg 
ggcgtcgtg tatactttt tctgagact tacaaccga 
gcatgtact tcatcttat tttgcttgt aaatattat 
gctaaaatt gaaattgac tgaattaaa actctagat 
atgaatcaa tgtgtttta ttgttttga ttacaatgt 
tatgtcacg cgttaagat tgttaaata taaaaaata 
tacatcaag aaacgacta gaaggagac tagacctct 
taattaaga taatataaa aatatcaat tgcatcaca 
ttgaaaata gttaggcaa acacaaacc aaattccac 
tggcagtaa taaaataag tcagacttg ttttaaaaa 
taagttgaa aaaccgatc acttcgtga atattgaaa 
ctagaagtt atgtaaaat tttcgatgg ttttttgtc 
tctcggacc acgatgttc gtaattgga tgtttccca 
attatgact ctttttgca tattccttg attgtgaag 
tgtttccac gaggggacg tggtaaaaa tacacctaa 
aaaacaatg aaaatcgga actacggag gattaaatg 
ttaggagat acgaccccg cgtacaaat caatcaatt 
tttttttaa ttatgaaga atatttcaa aaatgtgac 
gcatggtaa taaacagca aacagaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY