Status
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Gene information
General information
TC007101 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9551712..9570201
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007101
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01124

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007101 RA
      MFPKCVSGA STVSQLPKL FFLFSVSQL VSSSAVPED 
      WVSGSPFSH FAPDDSRAD TFTSAYLNV SNDGVRWDK 
      TEVGRYGGG YVGPAFGVL VHVAAENNP DDHTGCKLP 
      LHSTRSNRK LPPPGEPWI ALIKRGRCN FEVKVENAF 
      RSNAAGVLV YNDRDSATL DKMKLSSDS GRNISAVFT 
      YKWKGEDLA RLAENDSKV YIHITIASH TSSRTANIN 
      RTSVLFVSI TFIVLMIIS LAWLVFYYV QRFRYIHAK 
      DRLSRRLGN AAKKALSKI PTKNIKSED KEVQGDGEC 
      CAICIEPYK ICDILRILP CGHEFHKSC IDPWLLEHR 
      TCPMCKMDI LKHYGFVFS GSQESILQM DVEEIVVLD 
      STESRSTSP RRGGGISPL PEIRAVVIA DRQRLFESS 
      ADDESSRSS TPDEMTPSL SQARQYPVR QDLCVSCIA 
      AKAAAALSS SQSKEMSTS DEEQDSNRP VLSVNNQSD 
      DK
    CDS sequence
    • >TC007101 RA
      atgttcccc aagtgtgtt agtggtgca agcaccgtg 
      tcccagctc cccaaactg ttctttttg ttctccgtt 
      agccagttg gtgtcttct tcagcagtg cccgaggac 
      tgggtgtcg ggatccccc ttctcgcac ttcgcgccg 
      gacgacagc agggccgac actttcacg tcggcttac 
      ctgaacgtg tccaacgat ggagtgcgt tgggacaag 
      acggaggtg ggccgctat gggggaggc tacgtgggg 
      cccgccttc ggggtgctg gtgcacgtg gcggccgag 
      aacaacccc gacgaccac accgggtgc aagctcccg 
      ctgcacagc accaggtcc aatcggaag ttgccgccg 
      ccaggggag ccctggatc gccttgatc aagagaggc 
      cgctgcaac tttgaagtg aaggtcgag aacgccttc 
      aggagcaac gctgcgggg gttctggtc tataatgat 
      agggactcc gcgactttg gacaagatg aagttgtcc 
      agtgactcc ggacgtaat ataagcgcc gtgtttacc 
      tacaaatgg aagggagaa gacttggcc aggttggct 
      gaaaatgat tccaaagtt tacattcac atcactatt 
      gcgagccac acgtcctca cggactgcc aacatcaat 
      agaacatct gtgttattt gtctccatc acgtttatt 
      gttttgatg atcatatcc ttggcgtgg ttggtgttc 
      tactacgtg cagaggttt agatatatc cacgcaaaa 
      gacagactg tcgcgaaga ctgggaaat gcagcaaag 
      aaagcgtta tctaaaatc ccaacgaaa aacatcaaa 
      tccgaggac aaagaggtt caaggcgac ggggaatgt 
      tgcgctatt tgcattgaa ccttacaaa atatgtgat 
      attttacgc atattacct tgcggtcat gagttccat 
      aagagctgc attgatcct tggttgctg gaacatcga 
      acatgtcca atgtgtaaa atggacatc ctcaagcat 
      tatggattt gtgttttcc ggtagtcaa gaaagcatc 
      ctacagatg gacgtggag gaaatcgta gtgttagac 
      tcgacagag agtcgttca actagtcca agaaggggc 
      ggcggtatc agtcctttg ccggagata cgggccgtt 
      gtcatcgct gaccgtcag cgcctgttc gagagttcg 
      gcggacgac gaaagcagc agatcttcc acccctgac 
      gaaatgact ccgagttta tcgcaggca agacagtac 
      cccgtcaga caggacctt tgcgtgagc tgtatagcg 
      gcaaaggcg gccgcggcc ctgtcgagc agccaaagc 
      aaggaaatg tcaacttct gacgaggaa caagactcc 
      aaccggccg gtgctttca gtgaacaat caaagtgat 
      gataaataa
    mRNA sequence
    • >TC007101 RA
      attcagttg tgtggaaca cttgagcga gacggttgt 
      ggagcccca gccgtgcgc gagttcgtc gacttcaca 
      ggaaaagtg ctgtttgaa caaagaacg tcacttttc 
      agtgtggaa agtgcccct ccagcatga atgttccca 
      tctaacccg cgaagatgt tccccaagt gtgttagtg 
      gtgcaagca ccgtgtccc agctcccca aactgttct 
      ttttgttct ccgttagcc agttggtgt cttcttcag 
      cagtgcccg aggactggg tgtcgggat cccccttct 
      cgcacttcg cgccggacg acagcaggg ccgacactt 
      tcacgtcgg cttacctga acgtgtcca acgatggag 
      tgcgttggg acaagacgg aggtgggcc gctatgggg 
      gaggctacg tggggcccg ccttcgggg tgctggtgc 
      acgtggcgg ccgagaaca accccgacg accacaccg 
      ggtgcaagc tcccgctgc acagcacca ggtccaatc 
      ggaagttgc cgccgccag gggagccct ggatcgcct 
      tgatcaaga gaggccgct gcaactttg aagtgaagg 
      tcgagaacg ccttcagga gcaacgctg cgggggttc 
      tggtctata atgataggg actccgcga ctttggaca 
      agatgaagt tgtccagtg actccggac gtaatataa 
      gcgccgtgt ttacctaca aatggaagg gagaagact 
      tggccaggt tggctgaaa atgattcca aagtttaca 
      ttcacatca ctattgcga gccacacgt cctcacgga 
      ctgccaaca tcaatagaa catctgtgt tatttgtct 
      ccatcacgt ttattgttt tgatgatca tatccttgg 
      cgtggttgg tgttctact acgtgcaga ggtttagat 
      atatccacg caaaagaca gactgtcgc gaagactgg 
      gaaatgcag caaagaaag cgttatcta aaatcccaa 
      cgaaaaaca tcaaatccg aggacaaag aggttcaag 
      gcgacgggg aatgttgcg ctatttgca ttgaacctt 
      acaaaatat gtgatattt tacgcatat taccttgcg 
      gtcatgagt tccataaga gctgcattg atccttggt 
      tgctggaac atcgaacat gtccaatgt gtaaaatgg 
      acatcctca agcattatg gatttgtgt tttccggta 
      gtcaagaaa gcatcctac agatggacg tggaggaaa 
      tcgtagtgt tagactcga cagagagtc gttcaacta 
      gtccaagaa ggggcggcg gtatcagtc ctttgccgg 
      agatacggg ccgttgtca tcgctgacc gtcagcgcc 
      tgttcgaga gttcggcgg acgacgaaa gcagcagat 
      cttccaccc ctgacgaaa tgactccga gtttatcgc 
      aggcaagac agtaccccg tcagacagg acctttgcg 
      tgagctgta tagcggcaa aggcggccg cggccctgt 
      cgagcagcc aaagcaagg aaatgtcaa cttctgacg 
      aggaacaag actccaacc ggccggtgc tttcagtga 
      acaatcaaa gtgatgata aataagaca gttttctat 
      tacggtggt gcagcagta gtcgttagg actgccgaa 
      actttgtga tatatttct agtggttaa gtttttatt 
      ttgtccaat acgaataaa gggtgcttt cgtcccaaa 
      tactaacaa taatttatc gttcgctag aattcatat 
      tctagtgta agtaaatag atctctcgc gacgagttg 
      attccaatt ttccaagag attacatcc aacttattg 
      ggcttggga actcaatca agactcctg ttttgtttg 
      actatttgt tatcaagcg aacgaatga cgcgtttct 
      gaaggataa caagctgtt actttaatc gaaaataaa 
      ttaggagtt ttatgattt tgcttcgcc caagcgtga 
      ctttagtat gaagcgacg tgcttaata tttaaacta 
      ctgttcgca gtagtgctt ccaattcac attgttatt 
      gtctgctgg acaatgttt gtttgtgga aaacgaatt 
      ttcttctat aaacgttat aattacaat acaaatctt 
      aatactgtg ccttgtaaa tatctacat ttgtgatgt 
      acgtgatgc cccgcacga tgtaggtat gtgagcagt 
      tttgttatt tatctgtga ttgttaacg tttgttagg 
      ggcgtcgtg tatactttt tctgagact tacaaccga 
      gcatgtact tcatcttat tttgcttgt aaatattat 
      gctaaaatt gaaattgac tgaattaaa actctagat 
      atgaatcaa tgtgtttta ttgttttga ttacaatgt 
      tatgtcacg cgttaagat tgttaaata taaaaaata 
      tacatcaag aaacgacta gaaggagac tagacctct 
      taattaaga taatataaa aatatcaat tgcatcaca 
      ttgaaaata gttaggcaa acacaaacc aaattccac 
      tggcagtaa taaaataag tcagacttg ttttaaaaa 
      taagttgaa aaaccgatc acttcgtga atattgaaa 
      ctagaagtt atgtaaaat tttcgatgg ttttttgtc 
      tctcggacc acgatgttc gtaattgga tgtttccca 
      attatgact ctttttgca tattccttg attgtgaag 
      tgtttccac gaggggacg tggtaaaaa tacacctaa 
      aaaacaatg aaaatcgga actacggag gattaaatg 
      ttaggagat acgaccccg cgtacaaat caatcaatt 
      tttttttaa ttatgaaga atatttcaa aaatgtgac 
      gcatggtaa taaacagca aacagaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY