| General information | |||
| TC007098 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9592264..9596961 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007098 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01123 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC007098 RA
MSVIRKIVR VKTINKKLA AAFTSRRPY SNQSSQFIL HSDLEDVEI PKVSLDQYL FEKTHNWPQ RIATECAAT GRKYTYEEI QTKSINLNR NLRKKLKLQ KGDVVALLL PNSPEFIMA TIGALKAGL VVTTLNPIY TPDEIARQL KDSSTKAII TFVDFYELA KASANLTQS QINILTIKT QQGQAIPQG ALNFDEFTE PCDYPDVPP PDTNDIAFL PYSSGTTGL PKGVQLSHR NILANLCQF NARELSVIQ DTTQEHQDV IPAVLPKFH IYGLTATTL HLFYKGCKT VAISKFSPE GYLQTLRKY KPDVIFVAP PLVLFLASH PSVTSNDLQ SIRSVVSGA APLGALDEE RFITKAQKD INILQGYGL TETSPMVAM TRAALKKLP NSSGTIGRP VSNTSVKII DPNDPNETP LGANTTGEL VVKGPQVMK GYHNRPEET RDAFTKDGW FRTGDMMYY DDNKLLFVS DRLKELIKV KGFQVPPAE LEEIIRDFP EVKDAAVIG VPHPKDGEV PRAYIVGKN VDVNKLEEF VAQKVAPYK RLRGGIEIV ESIPKNATG KILRRALKE EFEKKMK
- >TC007098 RA
atgtctgtg atacgtaaa atcgtgcga gtgaaaact ataaataaa aaactcgct gcagcattc acttctcgc cgaccgtat tcaaaccag tcgtcgcag tttatcctg cattccgac ctagaagat gtggaaata cctaaggtt tcacttgat cagtatctg ttcgaaaaa acgcacaac tggccccag cggattgcc acggaatgt gccgccact ggccggaaa tatacttac gaagagatt caaaccaaa agcatcaat ttgaatcga aatctgcgc aaaaaactc aaattgcaa aaaggggat gttgtcgcg cttttgttg cccaattcg ccagagttt atcatggcc acgattggg gcacttaag gctggtctg gttgtaacc acattgaac ccaatttac actcctgat gaaattgca cgccagttg aaagactcg tcaacaaaa gccataata acgtttgtc gatttttac gaactggcc aaagcttca gccaattta actcaatca cagatcaac attttaaca atcaagacc cagcaaggc caagccatc ccacaaggt gctttaaat tttgacgaa tttaccgaa ccttgtgat tatccggac gtgccaccg cccgatacc aatgatatt gcattttta ccatactct agtgggaca accggcctt cccaaaggc gtccaactc agccacagg aatattttg gcgaatttg tgccagttt aacgccagg gaattgtcc gttattcaa gataccact caggagcac caagacgtg atcccggcc gttttaccc aaattccac atctacggt ctgactgca acaaccctg catttattc tacaaaggg tgcaaaacc gtggccatt tcgaaattc tctccagaa ggctacttg caaactttg cgcaaatac aaacccgac gtgatattc gtggcgccc ccacttgtg ctatttctt gcatcgcac ccttcggtt acatcaaac gaccttcag tccatccga tctgtagtt tctggggcc gctcctttg ggtgccctc gatgaggaa cgtttcata acaaaagcc cagaaagac atcaacatt ttgcaaggc tatggcctt acggaaacc tccccaatg gtcgcaatg acccgtgcc gcccttaaa aaactccct aattcaagc ggtaccatt ggccggcct gtttccaac acttcggtc aaaatcatc gacccaaat gacccaaat gaaacacct ttgggagct aacacaacc ggggaactg gtggtcaaa gggccgcaa gtcatgaaa gggtaccat aaccgtccc gaggaaact cgggacgct tttacgaaa gatgggtgg ttccgtacc ggagatatg atgtattac gatgacaac aaactgttg tttgttagc gatcgattg aaagagctg attaaagtt aaagggttt caagtacca ccagccgag ttggaggag atcattagg gatttcccc gaagttaag gacgccgct gtgattggg gtgccgcat ccgaaggac ggggaagta ccgagggct tacattgtg ggcaaaaac gtggatgtg aataaattg gaggagttt gttgcacag aaagtggcg ccttataag cgcctgaga ggggggatt gaaattgtt gaaagtatc cccaaaaat gccaccggg aaaatttta agacgcgct ttgaaagaa gaattcgaa aagaagatg aagtag
- >TC007098 RA
aattagcat ttttaatat tgccagtcg actcctgga gaaggggca acgcaaacg cgctacgtt agtgcaaaa acatacagt ggcagtgtc tacatcggt tgtcatgtc tgtgatacg taaaatcgt gcgagtgaa aactataaa taaaaaact cgctgcagc attcacttc tcgccgacc gtattcaaa ccagtcgtc gcagtttat cctgcattc cgacctaga agatgtgga aatacctaa ggtttcact tgatcagta tctgttcga aaaaacgca caactggcc ccagcggat tgccacgga atgtgccgc cactggccg gaaatatac ttacgaaga gattcaaac caaaagcat caatttgaa tcgaaatct gcgcaaaaa actcaaatt gcaaaaagg ggatgttgt cgcgctttt gttgcccaa ttcgccaga gtttatcat ggccacgat tggggcact taaggctgg tctggttgt aaccacatt gaacccaat ttacactcc tgatgaaat tgcacgcca gttgaaaga ctcgtcaac aaaagccat aataacgtt tgtcgattt ttacgaact ggccaaagc ttcagccaa tttaactca atcacagat caacatttt aacaatcaa gacccagca aggccaagc catcccaca aggtgcttt aaattttga cgaatttac cgaaccttg tgattatcc ggacgtgcc accgcccga taccaatga tattgcatt tttaccata ctctagtgg gacaaccgg ccttcccaa aggcgtcca actcagcca caggaatat tttggcgaa tttgtgcca gtttaacgc cagggaatt gtccgttat tcaagatac cactcagga gcaccaaga cgtgatccc ggccgtttt acccaaatt ccacatcta cggtctgac tgcaacaac cctgcattt attctacaa agggtgcaa aaccgtggc catttcgaa attctctcc agaaggcta cttgcaaac tttgcgcaa atacaaacc cgacgtgat attcgtggc gcccccact tgtgctatt tcttgcatc gcacccttc ggttacatc aaacgacct tcagtccat ccgatctgt agtttctgg ggccgctcc tttgggtgc cctcgatga ggaacgttt cataacaaa agcccagaa agacatcaa cattttgca aggctatgg ccttacgga aacctcccc aatggtcgc aatgacccg tgccgccct taaaaaact ccctaattc aagcggtac cattggccg gcctgtttc caacacttc ggtcaaaat catcgaccc aaatgaccc aaatgaaac acctttggg agctaacac aaccgggga actggtggt caaagggcc gcaagtcat gaaagggta ccataaccg tcccgagga aactcggga cgcttttac gaaagatgg gtggttccg taccggaga tatgatgta ttacgatga caacaaact gttgtttgt tagcgatcg attgaaaga gctgattaa agttaaagg gtttcaagt accaccagc cgagttgga ggagatcat tagggattt ccccgaagt taaggacgc cgctgtgat tggggtgcc gcatccgaa ggacgggga agtaccgag ggcttacat tgtgggcaa aaacgtgga tgtgaataa attggagga gtttgttgc acagaaagt ggcgcctta taagcgcct gagaggggg gattgaaat tgttgaaag tatccccaa aaatgccac cgggaaaat tttaagacg cgctttgaa agaagaatt cgaaaagaa gatgaagta gtgttaagt caaaaaatg tttaaggaa ggcttgttt taaaaacag acaatagca aactatgtt atttgcgta acaattaaa catgtaaca agttctcaa aagtgtaaa aataaaata aattaagca cagtcacgc acaataaac atgatccct tgac
