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Gene information
General information
TC007098 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 9592264..9596961
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007098
    iBeetle screen iB_01123
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007098 RA
      MSVIRKIVR VKTINKKLA AAFTSRRPY SNQSSQFIL 
      HSDLEDVEI PKVSLDQYL FEKTHNWPQ RIATECAAT 
      GRKYTYEEI QTKSINLNR NLRKKLKLQ KGDVVALLL 
      PNSPEFIMA TIGALKAGL VVTTLNPIY TPDEIARQL 
      KDSSTKAII TFVDFYELA KASANLTQS QINILTIKT 
      QQGQAIPQG ALNFDEFTE PCDYPDVPP PDTNDIAFL 
      PYSSGTTGL PKGVQLSHR NILANLCQF NARELSVIQ 
      DTTQEHQDV IPAVLPKFH IYGLTATTL HLFYKGCKT 
      VAISKFSPE GYLQTLRKY KPDVIFVAP PLVLFLASH 
      PSVTSNDLQ SIRSVVSGA APLGALDEE RFITKAQKD 
      INILQGYGL TETSPMVAM TRAALKKLP NSSGTIGRP 
      VSNTSVKII DPNDPNETP LGANTTGEL VVKGPQVMK 
      GYHNRPEET RDAFTKDGW FRTGDMMYY DDNKLLFVS 
      DRLKELIKV KGFQVPPAE LEEIIRDFP EVKDAAVIG 
      VPHPKDGEV PRAYIVGKN VDVNKLEEF VAQKVAPYK 
      RLRGGIEIV ESIPKNATG KILRRALKE EFEKKMK
    CDS sequence
    • >TC007098 RA
      atgtctgtg atacgtaaa atcgtgcga gtgaaaact 
      ataaataaa aaactcgct gcagcattc acttctcgc 
      cgaccgtat tcaaaccag tcgtcgcag tttatcctg 
      cattccgac ctagaagat gtggaaata cctaaggtt 
      tcacttgat cagtatctg ttcgaaaaa acgcacaac 
      tggccccag cggattgcc acggaatgt gccgccact 
      ggccggaaa tatacttac gaagagatt caaaccaaa 
      agcatcaat ttgaatcga aatctgcgc aaaaaactc 
      aaattgcaa aaaggggat gttgtcgcg cttttgttg 
      cccaattcg ccagagttt atcatggcc acgattggg 
      gcacttaag gctggtctg gttgtaacc acattgaac 
      ccaatttac actcctgat gaaattgca cgccagttg 
      aaagactcg tcaacaaaa gccataata acgtttgtc 
      gatttttac gaactggcc aaagcttca gccaattta 
      actcaatca cagatcaac attttaaca atcaagacc 
      cagcaaggc caagccatc ccacaaggt gctttaaat 
      tttgacgaa tttaccgaa ccttgtgat tatccggac 
      gtgccaccg cccgatacc aatgatatt gcattttta 
      ccatactct agtgggaca accggcctt cccaaaggc 
      gtccaactc agccacagg aatattttg gcgaatttg 
      tgccagttt aacgccagg gaattgtcc gttattcaa 
      gataccact caggagcac caagacgtg atcccggcc 
      gttttaccc aaattccac atctacggt ctgactgca 
      acaaccctg catttattc tacaaaggg tgcaaaacc 
      gtggccatt tcgaaattc tctccagaa ggctacttg 
      caaactttg cgcaaatac aaacccgac gtgatattc 
      gtggcgccc ccacttgtg ctatttctt gcatcgcac 
      ccttcggtt acatcaaac gaccttcag tccatccga 
      tctgtagtt tctggggcc gctcctttg ggtgccctc 
      gatgaggaa cgtttcata acaaaagcc cagaaagac 
      atcaacatt ttgcaaggc tatggcctt acggaaacc 
      tccccaatg gtcgcaatg acccgtgcc gcccttaaa 
      aaactccct aattcaagc ggtaccatt ggccggcct 
      gtttccaac acttcggtc aaaatcatc gacccaaat 
      gacccaaat gaaacacct ttgggagct aacacaacc 
      ggggaactg gtggtcaaa gggccgcaa gtcatgaaa 
      gggtaccat aaccgtccc gaggaaact cgggacgct 
      tttacgaaa gatgggtgg ttccgtacc ggagatatg 
      atgtattac gatgacaac aaactgttg tttgttagc 
      gatcgattg aaagagctg attaaagtt aaagggttt 
      caagtacca ccagccgag ttggaggag atcattagg 
      gatttcccc gaagttaag gacgccgct gtgattggg 
      gtgccgcat ccgaaggac ggggaagta ccgagggct 
      tacattgtg ggcaaaaac gtggatgtg aataaattg 
      gaggagttt gttgcacag aaagtggcg ccttataag 
      cgcctgaga ggggggatt gaaattgtt gaaagtatc 
      cccaaaaat gccaccggg aaaatttta agacgcgct 
      ttgaaagaa gaattcgaa aagaagatg aagtag
    mRNA sequence
    • >TC007098 RA
      aattagcat ttttaatat tgccagtcg actcctgga 
      gaaggggca acgcaaacg cgctacgtt agtgcaaaa 
      acatacagt ggcagtgtc tacatcggt tgtcatgtc 
      tgtgatacg taaaatcgt gcgagtgaa aactataaa 
      taaaaaact cgctgcagc attcacttc tcgccgacc 
      gtattcaaa ccagtcgtc gcagtttat cctgcattc 
      cgacctaga agatgtgga aatacctaa ggtttcact 
      tgatcagta tctgttcga aaaaacgca caactggcc 
      ccagcggat tgccacgga atgtgccgc cactggccg 
      gaaatatac ttacgaaga gattcaaac caaaagcat 
      caatttgaa tcgaaatct gcgcaaaaa actcaaatt 
      gcaaaaagg ggatgttgt cgcgctttt gttgcccaa 
      ttcgccaga gtttatcat ggccacgat tggggcact 
      taaggctgg tctggttgt aaccacatt gaacccaat 
      ttacactcc tgatgaaat tgcacgcca gttgaaaga 
      ctcgtcaac aaaagccat aataacgtt tgtcgattt 
      ttacgaact ggccaaagc ttcagccaa tttaactca 
      atcacagat caacatttt aacaatcaa gacccagca 
      aggccaagc catcccaca aggtgcttt aaattttga 
      cgaatttac cgaaccttg tgattatcc ggacgtgcc 
      accgcccga taccaatga tattgcatt tttaccata 
      ctctagtgg gacaaccgg ccttcccaa aggcgtcca 
      actcagcca caggaatat tttggcgaa tttgtgcca 
      gtttaacgc cagggaatt gtccgttat tcaagatac 
      cactcagga gcaccaaga cgtgatccc ggccgtttt 
      acccaaatt ccacatcta cggtctgac tgcaacaac 
      cctgcattt attctacaa agggtgcaa aaccgtggc 
      catttcgaa attctctcc agaaggcta cttgcaaac 
      tttgcgcaa atacaaacc cgacgtgat attcgtggc 
      gcccccact tgtgctatt tcttgcatc gcacccttc 
      ggttacatc aaacgacct tcagtccat ccgatctgt 
      agtttctgg ggccgctcc tttgggtgc cctcgatga 
      ggaacgttt cataacaaa agcccagaa agacatcaa 
      cattttgca aggctatgg ccttacgga aacctcccc 
      aatggtcgc aatgacccg tgccgccct taaaaaact 
      ccctaattc aagcggtac cattggccg gcctgtttc 
      caacacttc ggtcaaaat catcgaccc aaatgaccc 
      aaatgaaac acctttggg agctaacac aaccgggga 
      actggtggt caaagggcc gcaagtcat gaaagggta 
      ccataaccg tcccgagga aactcggga cgcttttac 
      gaaagatgg gtggttccg taccggaga tatgatgta 
      ttacgatga caacaaact gttgtttgt tagcgatcg 
      attgaaaga gctgattaa agttaaagg gtttcaagt 
      accaccagc cgagttgga ggagatcat tagggattt 
      ccccgaagt taaggacgc cgctgtgat tggggtgcc 
      gcatccgaa ggacgggga agtaccgag ggcttacat 
      tgtgggcaa aaacgtgga tgtgaataa attggagga 
      gtttgttgc acagaaagt ggcgcctta taagcgcct 
      gagaggggg gattgaaat tgttgaaag tatccccaa 
      aaatgccac cgggaaaat tttaagacg cgctttgaa 
      agaagaatt cgaaaagaa gatgaagta gtgttaagt 
      caaaaaatg tttaaggaa ggcttgttt taaaaacag 
      acaatagca aactatgtt atttgcgta acaattaaa 
      catgtaaca agttctcaa aagtgtaaa aataaaata 
      aattaagca cagtcacgc acaataaac atgatccct 
      tgac